简介：Severalbacterialstrainswereisolatedfromdifferentrhizospheres.Amongthese,strainPDY7exhibitedstrongantibacterialactivityagainstthericebacterialblight(BB)pathogenXanthomonasoryzaepv.oryzae(Xoo)bythelaboratorydualplateassays.TheantibacterialpropertyofthestrainPDY7wasfurtherinvestigatedfortheproductionof2,4-diacetylphloroglucinol(DAPG),whichamplifiedacharacteristicof629-bpDNAfragmentbyPCR-basedscreeningmethodusingphlDprimers.TheapplicationofphlDpositivestrainswascarefullyevaluatedfordiseasecontrolandgrowthpromotionofriceplantsunderfieldconditions.TheselectedstrainPDY7suppressedthericeBBby58.83%and51.88%underglasshouseandfieldconditions,respectively.Inaddition,thestrainPDY7showedsignificanttwo-foldincreaseinrootlength(18.08cm),shootlength(29.81cm),andgrainyield(96.07g).StrainPDY7promotedthegrowthofriceplantsbyproductionofindole-3-aceticacid(IAA),whichwasdeterminedbyhighperformanceliquidchromatography(HPLC)analysis.OurfindingssuggestthatPDY7belongstotheP.fluorescensgroupandcanserveaspotentialbiocontrolofBBaswellasbiofertilizeragentforgrowthpromotionofrice.

简介：自从1980年代，为抵抗繁殖到细菌的老家的米饭很快在中国一直在进行。基因Xa4主要在三线的indica混血儿和较普通活字大一倍的混合米饭被使用。疾病是‘quiet'在中国的20年了，然而，在最近的年里，它逐渐地出现了并且在在Changjiang河山谷与最新释放的米饭变化种的地里是流行的。在这种情况下，科学家们不可避免地提出了几个问题：什么并且什么引起苏醒我们应该下次做？或抵抗仍然在米饭正在引起主要目的之一改进？我们在抵抗上采取哪个途径繁殖以便抵抗将是更持久的，并且抵抗基因将更高效地被使用？包含传统的方法，分子的帮助标记的选择，和转基因的技术的联合策略应该把一个新时代带到引起节目的细菌的老家抵抗混血儿米饭。这评论简短也在与拓宽抵抗基因的细菌的老家抵抗，和合适的利用有关的问题上讨论并且商讨，可得到的抵抗基因的交替的种；并且甚至在亚洲在中国理解细菌的病原体的剧毒的人口。

简介：抗菌剂肽是有抗菌剂活动的多肽。从中国虾(FenneropenaeusChinensis)的抗菌剂肽基因Np3和Np5集成于OryzasativaL。subsp。装饰用的梨树cv。由Agrobacterium的Aichiashahi调停了转变系统。PCR分析证明Np3和Np5的积极比率分别地在T0产生是36%和45%。RT-PCR分析证明抗菌剂肽基因在T1产生被表示，并且在转基因的植物和非转基因的植物之间的农学的特点地没有明显的差别。四Np3和Np5在T1产生的转基因的线与Xanthomonasoryzaepv被接种。oryzae紧张CR4，和所有四根转基因的线显著地提高了抵抗到紧张CR4引起的细菌的老家。Np5转基因的线也显示出更高的抵抗到紧张JS97-2，Zhe173和OS-225引起的细菌的老家。有Np5基因力量的转基因的线拥有宽广光谱抵抗到米饭，这被建议细菌的老家。

简介：Throughrecurrentbackcrossingincombinationwithmolecularmarker-assistedselection(MAS),restorerlinesR8006andRl176carryingXa-21,agenehavingbroad-spectrumresistancetoricebacterialleafblight,wereselected.BycrossingthetwolinestoCMSlineZhong9A,twonewhybridricecombinations,Zhongyou6andZhongyou1176weredeveloped.Thehybridsshowedhighresistancetodiseases,goodgrainqualityandhighyieldingpotentialinnationalandprovincialadaptabilityandyieldtrials.

简介：到鞘老家(SB)的瑞斯抵抗被多基因或量的特点loci(QTL)控制并且由植物影响了词法特点。qSB-9TQ和TAC1分别地，控制SB抵抗和tiller角度(TA)的TQ,被采用测试二基因的联合是否将进一步改进米饭SB抵抗和还原剂收益损失而非仅仅他们或两个都不之一。用二根近的isogenic线(无)，TAC1TQ被证实贡献SB抵抗。然而，它的效果不比qSB-9TQ。随后，二基因被介绍进二个商业米饭变化开发一系列无。带两TAC1TQ和qSB-9TQ比仅仅包含他们之一的无显示出更多的抵抗。每植物比较谷物收益(欺骗)在三个不同SB疾病条件下面，也就是细微、中等、严重的条件，带显然失去的两基因的无降低没有带仅仅TAC1TQ。在细微疾病状况下面，没有重要差别在形态学上被发现，开发并且欺骗除了在带他们的基因和任何一个的无之间的TA联系了特点，显示二基因没在米饭上有劣等的效果农学的特点。结果显示了那TAC1TQ和qSB-9TQ有高繁殖的潜力，和节节上升的SB抵抗QTL和词法特点QTL是在改进米饭SB抵抗的一条潜在的途径。

简介：TheinteractionbetweenricehostanditspathogenXanthomonasoryzaepv.oryzae(Xoo)atcellularlevelwasstudiedbyusingaresistantsomaclonalmutantHX-3anditssusceptabledonorMinghui63.AfterinoculationwithXoostrainZhe173(Chinesepathotype|\).theactivityofsuperoxidedismutase(SOD)andperoxidase(POD)inthecallusofMinghui63wasincreaseddramatically,andtheactiveoxygen(O2^-)wasproducedatahigherrate;Meanwhile,thecallusgrewslowlywiththereductionofproteincontent.ComparedtotheactivityofSODandPOD.theproductionrateofO2^-andthefreshweightinHX-3callusvariedlittleaftertheinoculation.ItcouldbeproposedthatthereweregreatdifferencesbetweentheresistanceofHX-3andMighui63atcellularlevel.TherewasnodifferencedetectedconcerningresistancetobacterialleafblightinHX-3betweentheplantandthecallus.

简介：EighteenisolatesofRhizoctoniasolanicollectedfrominfectedriceplantsinfourdifferentlocationsofBangladeshwerestudiedbyusingmorphologicalcharactersandmolecularmarkers.AnastomosisstudywithareferenceisolateconfirmedthatalltheisolatesbelongedtoR.solani.Significantvariationwasobservedinsclerotialsize,shapeanddistribution.Un-weightedpairgroupmethodwitharithmeticmeandendrogramconstructedbasedontheGower’sgeneralsimilaritycoefficientshowedthattheseisolatesweregroupedintofourclustersatthe0.68similaritycoefficentaccordingtomorphologicalcharacters.ClusterIwasamajorclusterconsistingof13isolates,whileclustersⅡtoⅣconsistedof1or2isolates.Analysesbyvariablenumberoftandemrepeatandamplifiedfragmentlengthpolymorphismmarkersshowedthattheisolatesweregroupedintofiveandthreeclustersatasimilaritycoefficientof0.64and0.69,respectively.Althoughmostofthevariabilitywasfoundbetweenisolatesfromdifferentregionsasexpected,significantvariationwasobservedwithintheisolatescollectedfromsimilaragro-ecologicalregions.OurresultssuggestthepresenceofdifferentracesofR.solaniwithinthesamelocalgeographicregions.

简介：基于模拟FZ14从Zizanialatifolia(Griseb)导出的抵抗基因的顺序，一双特定的PCR教材FZ14P1/FZ14P2被设计孤立候选人疾病抵抗基因。pooled-PCR途径用教材对被采用屏蔽转变能干的人工的染色体(TAC)图书馆从Z导出的genomic。latifolia。积极TAC克隆(ZR1)被顺序分析获得并且证实。结果显示ZR1由类似的保存主题组成了，建议它能是kinase3a和GLPL(Gly-Leu-Pro-Leu)抵抗打的NBS-LRR的部分P环(kinase1a)，kinase2，基因。用Nipponbare成熟胚胎的调停Agrobacterium的转变，48独立转基因的T0植物的一个总数被获得。在他们之中，36植物对剧毒的细菌的老家紧张PXO71高度抵抗。结果显示ZR1包含至少一功能的细菌的老家抵抗基因。

简介：Ricesheathblight,causedbyRhizoctoniasolani(Kühn),isanotorioussoil-bornediseaseprevalentinmanyrice-growingregions.AlthoughseveralsporadicstudiesofmycovirusesinR.solaniAG-1IAhavebeenreportedforsinglestrainofR.solaniAG-1IA,therehavebeennoreportsdescribingthedistributionanddiversityofmycovirusesinnaturalpopulations.Inthisstudy,43R.solaniAG-1IAstrainscollectedfromdifferentlocationsinChinawereexaminedforthepresenceofdsRNAelementstoconfirmthepresenceofviralinfections.Electrophoretypesshowedthat16ofthe43fungalstrains(37.2%)containeddsRNAsthatcanbecharacterizedasviruses.Furthermore,thespecies-specificreversetranscriptionPCR(RT-PCR)showeddsRNAbandswithsimilarsizesdonotalwayscontainthesamevirusbutexistasmixedmycoviralinfections.Thus,ourfindingsindicatemycovirusesinfectingR.solaniAG-1IAinChinaarediverse,widespreadanduniversal.

简介：瑞斯鞘老家疾病(ShB)，由Rhizoctoniasolani引起了，产生重要谷物收益损失。现在的学习评估了积分的功效?，杆菌subtilis紧张MBI600的商业液体明确的表达，对米饭ShB并且为植物生长提升。在温室研究，积分的四木头集中(从2.2??????????????？？

简介：二细菌的老家(BB)抵抗基因，Xa21和Xa4，从IRBB24被介绍进杂交稻restorer线Mianhui725，它高度产生BB，由使用杂交和分子的帮助标记的选择技术。四根相应恢复系线通过与分子的标记测试R目标基因并且分析父母遗传背景被获得。有特定的紧张ofXanthomonasoryzaepv的四根线和他们的混血儿的接种。oryzae，P1，P6和七个代表汉语拉紧病理型，CⅠ-CⅦ，证明所有四根线和他们的混血儿是到BB的高度抵抗、介绍的宽广抵抗光谱。G46A/R207-2的混血儿显示了好农学的人物和高产潜力，并且R207-2被称为Shuhui207。

简介：Itisnecessarytounderstandthebacterialpopulationsassociatedwithricesoastoprovidemoreinformationandnaturalresourcesforeffectivemanagementofmajordiseasesinrice.Asurveyonscreeningandidentificationofgram-positivebacteriawasconductedduring1998-2004.Sevenhundredandfifty-sixricesampleswerecollectedfromZhejiang,Jiangsu,FujianandYunnanProvinces,China.Over1000bacterialisolateswereisolatedandtestedforcolonymorphology,pathogenicity,andsomecharacteristicsofbacteriologyincludingGramstaining,fluorescentpigmentonKingsmediumBandmicroscopicobservationforendospore.Togetherwithfivestandardreferencestrains,74representativegram-positivebacterialisolateswereconfirmedbyBiologandgaschromatographicanalysisoffattyacidmethylesters.FivebacterialspeciesofBacillusandotherthreegenerawereidentifiedandisolatesfromBacillussublitisandBacillusmegaterium,exhibitedthemosteffectiveinhibitionagainstthepathogensofsheathblightandbakanaediseaseofrice.AfewisolatesfromBacilluspumilusandBacillusmegateriumshowedweakvirulentonricetogetherwithsomevirulentisolates,dskshouldbeconsideredwhenisolatesfromthesespecieswerescreenedforbiocontrolagents.

简介：Thetransgenicrice,Zhongda2,whichwasgeneticallymodifiedfromanindicaricelineZhuxianBbyricechitinasegene(RC24),hadhighresistancetoricesheathblight(Rhizoctoniasolam)inlaboratoryandatwo-yearfieldexperiment.ThepathogencouldinvadesheathofZhongda2andinducesymptomsofthedisease.NodifferencewasnotedintimeofpenetrationorincubationperiodbetweenZhongda2andnon-transgenicricecontrol,ZhuxianB,butthehyphaelysatecouldbeobservedeadierthancontrol.Itsresistanceexpressedastoinhibitthegrowthofmyceliuminhosttissue.F1sfromZhongda2(♂)crossedwithotherfivenon-transgenicricelinesshowedhigherresistancethandonornon-transgenicparents,buttheresistancewasdifferentalongwiththedifferentmaternalparents.

简介：Twomajorbacterialblight(BB)resistancegenes(Xa21andxa13)andamajorgeneforblastresistance(Pi54)wereintrogressedintoanIndianricevarietyMTU1010throughmarker-assistedbackcrossbreeding.ImprovedSambaMahsuri(possessingXa21andxa13)andNLR145(possessingPi54)wereusedasdonorparents.Marker-assistedbackcrossingwascontinuedtillBC2generationwhereinPCRbasedfunctionalmarkersspecificfortheresistancegeneswereusedforforegroundselectionandasetofparentalpolymorphicmicrosatellitemarkerswereusedforbackgroundselectionateachstageofbackcrossing.SelectedBC2F1plantsfrombothcrosses,havingthehighestrecoveriesofMTU1010genome(90%and92%,respectively),wereintercrossedtoobtainintercrossF1(ICF1)plants,whichwerethenselfedtogenerate880ICF2plantspossessingdifferentcombinationsoftheBBandblastresistancegenes.AmongtheICF2plants,seventriplehomozygousplants(xa13xa13Xa21Xa21Pi54Pi54)withrecurrentparentgenomerecoveryrangingfrom82%to92%wereidentified.AllthesevenICF2plantsshowedhighresistanceagainstthebacterialblightdiseasewithalesionlengthsofonly0.53–2.28cm,1%–5%diseaseleafareasanddiseasescoringvaluesof‘1'or‘3'.ThesevenICF2plantswereselfedtogenerateICF3,whichwerethenscreenedforblastresistance,andallwereobservedtobehighlyresistanttothediseases.SeveralICF3linespossessinghighlevelofresistanceagainstBBandblast,coupledwithyield,grainqualityandplanttypeonparwithMTU1010wereidentifiedandadvancedforfurtherselectionandevaluation.

简介：ToconstructtheT-DNAinsertionalmutagenesistransformationsystemforricesheathblightpathogenRhizoctoniasolaniAG-1IA,thevirulentisolateGD118ofthispathogenwasselectedasaninitialisolatefortransformation.TheconditionsfortransformationofisolateGD118wereoptimizedinfiveaspects,i.e.pre-inductiontime,co-culturetime,acetosyringone(AS)concentrationattheco-culturephase,co-culturetemperatureandpHvalueofinductionsolidmedium(ISM)attheco-culturephase.Finally,asystem...

简介：Bacterialleafblightofrice(BLB),causedbyXanthomonasoryzaepv.oryzae,isoneofthemostdestructivediseasesinAsianricefields.Ahigh-qualityricevariety,LT2,wasusedastherecipientparent.IRBB21,whichcarriestheXa21gene,wasusedasthedonorparent.TheresistancegeneXa21wasintroducedintoLT2bymarker-assistedbackcrossing.ThreeXooraceswereusedtoinoculatetheimprovedlinesfollowingtheclippingmethod.ElevenBC3F3linescarryingXa21wereobtainedbasedonmolecularmarkersandagronomicperformance.The11linesweretheninoculatedwiththethreeXooraces.Allthe11improvedlinesshowedbetterresistancetoBLBthantherecipientparentLT2.BasedonthelevelofresistancetoBLBandtheiragronomicperformance,fivelines(BC3F35.1.5.1,BC3F35.1.5.12,BC3F38.5.6.44,BC3F39.5.4.1andBC3F39.5.4.23)wereselectedasthemostpromisingforcommercialrelease.Theseimprovedlinescouldcontributetoriceproductionintermsoffoodsecurity.