简介：EighteenisolatesofRhizoctoniasolanicollectedfrominfectedriceplantsinfourdifferentlocationsofBangladeshwerestudiedbyusingmorphologicalcharactersandmolecularmarkers.AnastomosisstudywithareferenceisolateconfirmedthatalltheisolatesbelongedtoR.solani.Significantvariationwasobservedinsclerotialsize,shapeanddistribution.Un-weightedpairgroupmethodwitharithmeticmeandendrogramconstructedbasedontheGower’sgeneralsimilaritycoefficientshowedthattheseisolatesweregroupedintofourclustersatthe0.68similaritycoefficentaccordingtomorphologicalcharacters.ClusterIwasamajorclusterconsistingof13isolates,whileclustersⅡtoⅣconsistedof1or2isolates.Analysesbyvariablenumberoftandemrepeatandamplifiedfragmentlengthpolymorphismmarkersshowedthattheisolatesweregroupedintofiveandthreeclustersatasimilaritycoefficientof0.64and0.69,respectively.Althoughmostofthevariabilitywasfoundbetweenisolatesfromdifferentregionsasexpected,significantvariationwasobservedwithintheisolatescollectedfromsimilaragro-ecologicalregions.OurresultssuggestthepresenceofdifferentracesofR.solaniwithinthesamelocalgeographicregions.

简介：solani孤立的Rhizoctonia的55件代表性的样品，从在四川省的五个不同生态的区域镇定、孤立，中国，为致病力和分子的基因变化被净化并且分析。菌丝的熔化测试表明除了isolateD42，几乎所有isolates属于AG-IIA组。另外，一些isolates正在衔接孤立，它能同时与几熔化组。致病力分析在上在vitro，叶子证实了一个重要致病力变化在测试孤立。55孤立然后被进一步的RAPD(随机放大的多态的DNA)分类进8个组在0.941的类似系数的簇分析。结果在四川省在某些生态的条件下面建议那，中国，大多数R。solani紧张是遗传上稳定的，但是一些急速地变化了。

简介：Ricesheathblight,causedbyRhizoctoniasolani(Kühn),isanotorioussoil-bornediseaseprevalentinmanyrice-growingregions.AlthoughseveralsporadicstudiesofmycovirusesinR.solaniAG-1IAhavebeenreportedforsinglestrainofR.solaniAG-1IA,therehavebeennoreportsdescribingthedistributionanddiversityofmycovirusesinnaturalpopulations.Inthisstudy,43R.solaniAG-1IAstrainscollectedfromdifferentlocationsinChinawereexaminedforthepresenceofdsRNAelementstoconfirmthepresenceofviralinfections.Electrophoretypesshowedthat16ofthe43fungalstrains(37.2%)containeddsRNAsthatcanbecharacterizedasviruses.Furthermore,thespecies-specificreversetranscriptionPCR(RT-PCR)showeddsRNAbandswithsimilarsizesdonotalwayscontainthesamevirusbutexistasmixedmycoviralinfections.Thus,ourfindingsindicatemycovirusesinfectingR.solaniAG-1IAinChinaarediverse,widespreadanduniversal.

简介：瑞斯鞘老家疾病(ShB)，由Rhizoctoniasolani引起了，产生重要谷物收益损失。现在的学习评估了积分的功效?，杆菌subtilis紧张MBI600的商业液体明确的表达，对米饭ShB并且为植物生长提升。在温室研究，积分的四木头集中(从2.2??????????????？？

简介：ToconstructtheT-DNAinsertionalmutagenesistransformationsystemforricesheathblightpathogenRhizoctoniasolaniAG-1IA,thevirulentisolateGD118ofthispathogenwasselectedasaninitialisolatefortransformation.TheconditionsfortransformationofisolateGD118wereoptimizedinfiveaspects,i.e.pre-inductiontime,co-culturetime,acetosyringone(AS)concentrationattheco-culturephase,co-culturetemperatureandpHvalueofinductionsolidmedium(ISM)attheco-culturephase.Finally,asystem...

简介：ThepresentstudywasdesignedtoisolateandcharacterizeapurifiedextractfromFusariumsolaniFG319,termedMFS(MetaboliteofFusariumsolaniFG319)thatshowedanti-atherosclerosisactivitybyinhibiting3-hydroxy-3-methylglutarylcoenzymeA(HMG-CoA)reductase.Responsesurfacemethodology(RSM)wasemployedtoachieveanimprovedyieldfromthefermentationmedium.Theinhibitingeffectoftheisolate,MFS,onHMG-CoAreductasewasgreaterthanthatofthepositivecontrol,lovastatin.TheaveragerecoveryofMFSandtherelativestandarddeviation(RSD)rangedbetween99.75%to101.18%,and0.31%to0.74%,respectively.TheRSDsintra-andinter-assayofthethreesamplesrangedfrom0.288%to2.438%,andfrom0.934%to2.383%,respectively.FromtheRSM,theconcentrationofinducer,cultivationtime,andculturetemperatureshadsignificanteffectsontheMFSproduction,withtheeffectofinducerconcentrationbeingmorepronouncedthatotherfactors.Inconclusion,theoptimalconditionsfortheMFSproductionwereachievedusingRSMandthatMFScouldbeexploredasananti-atherosclerosisagentbasedonitsabilitytoinhibitHMG-CoAreductase.