简介：增加的证据证明丝氨酸，threonine和酷氨酸残余上的蛋白质phosphorylation是在细菌的主要规章的translational以后修正。这评论在细菌的致病力集中于细菌的phosphoproteome的含意并且在phosphoproteomics和phosphorylation网络的连接加亮方法的最近的开发。在高精确性团spectrometry的最近的技术开发戏剧性地转变了proteomics并且使它可能一些彻底的地点特定的细菌的phosphoproteomes的描述。在一些细菌的phosphoproteomes的高许多酷氨酸phosphorylations在致病力建议他们的角色，特别在病原体主人相互作用的情况中；在细菌的邻蛋白质的高许多multi-phosphorylation地点是相对小的phosphorylation尺寸和蛋白质功能的精细的规定的指示物的赔偿。

简介：ThebacteriausedintheexperimentareThiobacillusFerrooxidansseparatedfromacidicminewaterinsulphidedeposits.Thechemoautotrophicbacteriacanactdirectlyonsulphidesandacceleratetheoxidationofsulphides.Theexperimentshowsthatthebacteria,asanimportantmicrobialfactorofgold’ssupergenousenrichmentwithintheoxidizedzonesofsulphidedeposits,arehelpfultodissolvegoldandsilverinferricsulphate.Inthebacterialoxidationprocess,theprecipitationofgoethiteisconcernedbothwiththeloweractivityofferricionsandwiththeexistenceofcarbonatesinsolution.Meanwhile,theacid-resistingandoxidizingabilityofthebacteriawillcertainlyleaduptoamicrobialwayoftreatingtheacidicminewater.

简介：Therapidincreasingnumberofcompletedbacterialgenomesprovidesagoodopportunitytocomparetheirproteomes.Thisstudywasundertakentospecificallycompareandcontrasttheirsecretomes—thefractionoftheproteomewithpredictedN-terminalsignalsequences,bothtypeIandtypeII.Atotalof176theoreticalbacterialproteomeswereexaminedusingtheExProtprogram.ComparedwiththeGram-positives,theGram-negativebacteriawerefound,onaverage,tocontainalargernumberofpotentialSec-dependentsequences.IntheGram-negativebacteriabutnotintheothers,therewasapositivecorrelationbetweenproteomesizeandsecretomesize,whiletherewasnocorrelationbetweensecretomesizeandpathogenicity.WithintheGram-negativebacteria,intracellularpathogenswerefoundtohavethesmallestsecretomes.However,thesecretomesofcertainbacteriadidnotfitintotheobservedpattern.Specifically,thesecretomeofBorreliaburgdoferihasanunusuallylargenumberofputativelipoproteins,andthesignalpeptidesofmycoplasmasshowclosersequencesimilaritytothoseoftheGramnegativebacteria.Ouranalysisalsosuggeststhatevenforatheoreticalminimalgenomeof300openreadingframes,afractionofthisgenepool(uptoamaximumof20%)maycodeforproteinswithSec-dependentsignalsequences.

简介：在过去几十年，从在整个世界的科学家以象小规章的RNA那样的新奇功能的单位拿了热切的兴趣，小开的读物框架，pseudogenes，transposons，integrase绑定attB/attP地点，为genomic复杂性在细菌的intergenic区域(IGR)以内并且在那些垃圾区域的分析重复元素。这里，我们开发了一个网服务者，命名Junker，详细说明为检验他们的长度分发，四象限的阴谋，GC百分比和重复便于IGR的深入的分析。在一个特别细菌的染色体的选择之上，物理染色体地图与选择作为多重loci被显示详细看兴趣的任何loci。另外，一个IGR统计模块被创造了并且在网服务者实现了分析IGR的长度分发并且理解由产生四象限的阴谋的越过染色体的IGR的混乱组织。建议的网服务者在URLhttp://pranag.physics.iisc.ernet.in/junker/是自由地可得到的。

简介：BacterialwiltcausedbyPseudomonassolanacearum,isaveryseriousdiseaseofEucalyptusinsouthernChina,mainlyinGuangdong,HainanandGuangxiprovinces.Itcausesthedeathofseedlingsandyoungtreesandthemortalitymayexceed90percentinsomesites.SusceptiblespeciesincludeE.grandis,E.urophylla,hybridsbetweenthetwoandE.citriodora.InnurseryandfieldtrialswithE.urophyllaandfieldtrialwithE.grandisxE.urophyllainoculationofectomycorrhizalfungiwasfoundtored...

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简介：自己准备的细菌的纤维素在Cr(VI)的吸附被使用。象吸附时间那样的性能参数的效果，pH，Cr(VI)上的吸附物剂量被调查。结果证明pH是吸附的效率的一个很重要的参数。Cr(VI)的移动率在pH的条件下面来临了到15%1.5，吸附物剂量1.0g獥Δ

简介：Diversityofbacteriawasstudiedindeep-seasedimentsfromtheShikokuBasinintheNorthwestPacificOceanbyPCR,RFLPandsequenceanalysisof16SrDNAandcomparingwithGenbankdatabase.BasedontheRFLPprofilegenerated,77clonesfromthe16SrDNAlibraryweredividedinto27types.Phylogeneticanalysisshowedthatthe27independentclonesfellintofourgroups:Proteobacteria(62.96%),Chloroflexi(14.81%),Planctomycetes(14.81%)andAcidobacteria(7.41%).Amongallsequencedclones,6wererelatedtothesulfurorsulfatemetabolismbacteriaandtheresultsalsodemonstratedthatsomebacteriaindeep-seasedimentshadrelationtomatter-energycirculation.

简介：Severalbacterialstrainswereisolatedfromdifferentrhizospheres.Amongthese,strainPDY7exhibitedstrongantibacterialactivityagainstthericebacterialblight(BB)pathogenXanthomonasoryzaepv.oryzae(Xoo)bythelaboratorydualplateassays.TheantibacterialpropertyofthestrainPDY7wasfurtherinvestigatedfortheproductionof2,4-diacetylphloroglucinol(DAPG),whichamplifiedacharacteristicof629-bpDNAfragmentbyPCR-basedscreeningmethodusingphlDprimers.TheapplicationofphlDpositivestrainswascarefullyevaluatedfordiseasecontrolandgrowthpromotionofriceplantsunderfieldconditions.TheselectedstrainPDY7suppressedthericeBBby58.83%and51.88%underglasshouseandfieldconditions,respectively.Inaddition,thestrainPDY7showedsignificanttwo-foldincreaseinrootlength(18.08cm),shootlength(29.81cm),andgrainyield(96.07g).StrainPDY7promotedthegrowthofriceplantsbyproductionofindole-3-aceticacid(IAA),whichwasdeterminedbyhighperformanceliquidchromatography(HPLC)analysis.OurfindingssuggestthatPDY7belongstotheP.fluorescensgroupandcanserveaspotentialbiocontrolofBBaswellasbiofertilizeragentforgrowthpromotionofrice.

简介：AbstractAccumulating evidence suggests that intestinal bacteria play an important role in the pathogenesis of colorectal cancer (CRC). Due to the complexity of the intestinal microbiome, identification of the specific causative microbial agents in CRC remains challenging, and the search for the causative microbial agents is intense. However, whether bacteria or their products can induce inflammation that results in tumorigenesis or directly causes CRC in humans is still not clear. This review will mainly focus on the progress of bacterial infection and CRC, and introduce the microbial contribution to the hallmarks of cancer. This article uses Salmonella and its chronic infection as an example to investigate a single pathogen and its role in the development of CRC, based on laboratory and epidemiological evidence. The bacterial infection leads to an altered intestinal microbiome. The review also discusses the dysfunction of the microbiome and the mechanism of host-microbial interactions, for example, bacterial virulence factors, key signaling pathways in the host, and microbial post-translational modifications in the tumorigenesis. Colonic carcinogenesis involves a progressive accumulation of mutations in a genetically susceptible host leading to cellular autonomy. Moving forward, more human data are needed to confirm the direct roles of bacterial infection in CRC development. Insights into the inhibiting infection will help to prevent cancer and develop strategies to restore the balance between host and microorganisms.

简介：Inthisstudy,athermophilicoil-degradingbacterialconsortiumKO8-2growingwithinthetemperaturerangeof45-65℃(with55℃beingtheoptimumtemperature)wasisolatedfromoil-contaminatedsoilofKaramayinXinjiang,China.Denaturinggradientgelelectrophoresis(DGGE)showedthattherewereninestrainsincludedinKO8-2,whichoriginatedfromthegeneraofBacillus,GeobacillusandClostridium.Theyallbelongedtothermophilicbacteria,andhadbeenpreviouslyprovedasdegradersofatleastonepetroleumfraction.ThecrudeoildegradedbyKO8-2wasanalyzedbyinfraredspectrophotometry,hydrocarbongrouptypeanalysisandgaschromatography.TheresultsindicatedthatthebacterialconsortiumKO8-2wasabletoutilize64.33%ofsaturates,27.06%ofaromatics,13.24%ofresinsandtheoilremovalefficiencyreachedupto58.73%at55℃whentheoilconcentrationwas10g/L.DetailedanalysisshowedthatKO8-2wasabletoutilizethehydrocarboncomponentsbeforeC19,andthen-alkanesrangingfromC20-C33weresignifi-cantlydegraded.TheratiosofnC17/PrandnC18/Phwere3.12and3.87,respectively,beforedegradation,whereasafterdegradationtheratiosreducedto0.21and0.38,respectively.Comparedwiththecontrolsample,theoilremovalefficiencyinKO8-2compostingreactorreached50.12%afteradegradationdurationof60days.

简介：自从1980年代，为抵抗繁殖到细菌的老家的米饭很快在中国一直在进行。基因Xa4主要在三线的indica混血儿和较普通活字大一倍的混合米饭被使用。疾病是‘quiet'在中国的20年了，然而，在最近的年里，它逐渐地出现了并且在在Changjiang河山谷与最新释放的米饭变化种的地里是流行的。在这种情况下，科学家们不可避免地提出了几个问题：什么并且什么引起苏醒我们应该下次做？或抵抗仍然在米饭正在引起主要目的之一改进？我们在抵抗上采取哪个途径繁殖以便抵抗将是更持久的，并且抵抗基因将更高效地被使用？包含传统的方法，分子的帮助标记的选择，和转基因的技术的联合策略应该把一个新时代带到引起节目的细菌的老家抵抗混血儿米饭。这评论简短也在与拓宽抵抗基因的细菌的老家抵抗，和合适的利用有关的问题上讨论并且商讨，可得到的抵抗基因的交替的种；并且甚至在亚洲在中国理解细菌的病原体的剧毒的人口。

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简介：DNAmismatchrepairguardstheintegrityofthegenomeofalmostallorganismsbycorrectingDNAbiosyntheticerrorsandbyensuringthefidelityofhomologousgeneticrecombination.MutLisoneoftheimportantproteinsinvolvedinmismatchrepairsystem.IthasbeensuggestedtofunctionasamastercoordinatorormolecularmatchmakerbecauseitinteractsphysicallywithMutS,theendonucleaseMutH,andDNAhelicaseUvrD.ItalsobindstoDNAandhasanATPaseactivity.MutLdefectivebacteriastrainshaveelevatedmutationratesandithasbeenreportedrecentlythatMutLdefectmayhaveanimportantimpactonbacterialevolution.

简介：抗菌剂肽是有抗菌剂活动的多肽。从中国虾(FenneropenaeusChinensis)的抗菌剂肽基因Np3和Np5集成于OryzasativaL。subsp。装饰用的梨树cv。由Agrobacterium的Aichiashahi调停了转变系统。PCR分析证明Np3和Np5的积极比率分别地在T0产生是36%和45%。RT-PCR分析证明抗菌剂肽基因在T1产生被表示，并且在转基因的植物和非转基因的植物之间的农学的特点地没有明显的差别。四Np3和Np5在T1产生的转基因的线与Xanthomonasoryzaepv被接种。oryzae紧张CR4，和所有四根转基因的线显著地提高了抵抗到紧张CR4引起的细菌的老家。Np5转基因的线也显示出更高的抵抗到紧张JS97-2，Zhe173和OS-225引起的细菌的老家。有Np5基因力量的转基因的线拥有宽广光谱抵抗到米饭，这被建议细菌的老家。

简介：AIM:ToanalyzetheassociationbetweenHelicobacterspp.andsomecommongutbacteriainpatientswithcholecystitis.METHODS:Anested-polymerasechainreaction(PCR),specificto16SrRNAofHelicobacterspp.wasperformedonparaffin-embeddedgallbladdersamplesof100cholecystitisand102controlcases.ThesampleswerealsoanalyzedforsomecommongutbacteriabyPCR.Positivesamplesweresequencedforspeciesidentification.RESULTS:HelicobacterDNAwasfoundinsevenoutof100casesofacuteandchroniccholecystitis.SequenceanalysisdisplayedHelicobacterpullorum(H.pullorum)insixcasesandHelicobacterpyloriinone;H.pullorumwasonlyfoundincaseswithmetaplasia.Controlsam-pleswerenegativeforHelicobacterspp.andsomecommongutbacteria.Therewasasignificantdifference(P=0.007)betweencholecystitisandcontrolsamplesforHelicobacterDNA.CONCLUSION:ApossiblerelationshipwasdetectedbetweenHelicobacterDNAandcholecystitis.Furtherserologicalandimmunohistochemicalstudiesareneededtosupportthesedata.

简介：AbstractAim:Toassesstheefficacyandsafetyofanalsubmucosalinjection(ASI)ofamikacininchronicbacterialprostatitis(CBP).Methods:FiftymaleoutpatientswithCBPwererandomlydiidedintotwogroups.ThirtycasesofASIgroupweregivenamikacin400mgdailybyASIfortentimesandtheothertwentycasesofintramuscularinjection(IM)groupweregiventhesamedrugdailybyIM,AllpatientswereevaluatedwithNIH-Chronicprostatitissymptomindex(NIH-CPSI),thebacteriacultureoftheexpressedprostatesecretion(EPS),proctoscopicexamination,rectalbiopsyandtheclinicalmanifestationwerecheckedatpretreatmentandonday7and90aftercessationoftherapy,Results:Thecurerate,apparenteffectiverateandeffectiverateofASIgroupandIMgroupwere33.3%vs5%(P<0.050,43.3%vs10%(P<0.05)and16.7%vs20%(P>0.05).respectively.ThescoreofNIH-CPSIinbothofASIgroupandIMgroupdecreasedsignificantly7daysaftercessationoftherapy,bothASIandIMofamikacincouldrelievesymptomswithinashorttime,However,3monthsaftercessationoftherapythescoreofNIH-CPSIinASIgroupcontinuedowninspiteofnosignificantdifferencescomparedwith7daysaftercessationoftheragy,butthescoreofIMgroupwasreboundnearlycolsedtolevelofpretreatmentat23.8±8.5andsignificantlyhigherthanthatofASIgroup.Theamountofwhitebloodcell(WBC)ofEPSinASIgroupincreasedslightlyat7daysaftercessationoftherapywithoutsignificantdifferencewithpretreatment(P>0.050,butitsignificantlydecreasedat3monthsaftercessationoftherapy,theamountofWBCofEPSinASIgroupwaslowerthanthatofIMgroupat3monthsaftercessationoftherapy(P<0.05).ProctoscopicexaminationofanalcanalwerenormalafterASItherapyandtherectumbiopsyshowednoobvioushistopathologicabnormalityatthesiteofinjectionexceptmildfocalsubmucosalinfiltrationoflymphocytesandplasmacellsat7daysaftercessationoftherapywhichdisappearedon3monthsaftercessationoftherapy.Allpatientshadnoevidentcomplicat

简介：Akindofluminousbacterialsensorsthatcanquicklydetecttheacutetoxicityofenvironmentalpollutantsweredeveloped.Themethodisbasedonthedetectionofthecellularlightofbrightluminousbacillusbymeansoffixingcellssoastodetectacutetoxicityofluminousbacillus.Thebacterialsensoriscomposedofimmobilizedfilmofphotobacteriumphosphoreum.Thesebacterialfilmsaresensitivetodetectingthetoxicoids,whicharedifficultorevenimpossibletobemeasuredbytraditionalanalyticalchemistrymethods.Thefilmsshouldbestoredat4℃andthestabilityofthesensorsexceeds1monthwithnomeasurabledeteriorationofthesignal.TheseresultsdemonstratethattheimmobilizedfilmofP.phosphreumcanbeusedtodeveloptheon-lineenvironmentalcontaminationmonitor.

简介：能够lysing的所有以前报导的细菌的种类有害水藻从有害水藻花蕾在发生了的沿海的环境被孤立。由于在一个海藻的花蕾的水藻细胞溶解细菌的低集中，由存在方法孤立水藻细胞溶解细菌是困难的。在这篇论文，二水藻细胞溶解细菌的紧张，P01；P03，从在在移开水藻是高度有效的海绵上使不能调动的一个简历系统被孤立；microcystins。他们的lysing模式；Microcystisaeruginosa上的效果被学习了。降级为M这二紧张处理的结果表演。当时，与一阶的反应给予的aeruginosa当模特儿集中在从0～1000μgL的范围的叶绿素[1]。降级率常数是0.1067，0.1274；0.2792为P01；0.0683，0.0744；0.02897为P03当细菌的密度是8.6×1时0[5]，8.6×10[6]；8.6×10[7]房间mL[1]分别地。而且，二细菌的紧张在M上有赞成lysin的效果不仅。aeruginosa，而且上小球藻；Scene-desmus。他们M上的lysin的效果。aeruginosa没要求物理房间到房间接触，但是由一个细胞外的产品的生产继续。细菌的紧张被16SrRNA基因的PCR扩大作为杆菌种类识别，强风分析，；有在GenBank核苷酸数据库的序列的比较。

简介：Throughrecurrentbackcrossingincombinationwithmolecularmarker-assistedselection(MAS),restorerlinesR8006andRl176carryingXa-21,agenehavingbroad-spectrumresistancetoricebacterialleafblight,wereselected.BycrossingthetwolinestoCMSlineZhong9A,twonewhybridricecombinations,Zhongyou6andZhongyou1176weredeveloped.Thehybridsshowedhighresistancetodiseases,goodgrainqualityandhighyieldingpotentialinnationalandprovincialadaptabilityandyieldtrials.