简介：

简介：AstatisticalthermodynamictheoryoflinearproteinsolutionswasproposedwiththeaidofalatticemodelandappliedtotypeⅠantifreezeprotein(AFPI)solutions.ThenumericalresultsforseveralAFPIsolutionsshowthattheGibbsfunctionofthesolutionhasaminimumatacertainproteinconcentration,buttheproteinchemicalpotentialincreaseswithincreasingtheconcentration.TheinfluencesoftemperatureandproteinchainlengthontheAFPIchemicalpotentialwerealsodiscussed.Theevaluationforthecolligativedepressionofthefreezingpointconfirmsthattheantifreezeactionshouldberecognizedasnon-colligative.Thetheoreticaldeductionfortheconcentrationdependenceofthethermalhysteresisactivitycoincidesqualitativelywiththepreviousexperimentalandtheoreticalresults.

简介：Azurins,awildtypeandageneticallymutantK27alteredone,wereimmobilizedonannealedgoldsurfaceandinvestigatedbymeansofatomicforcemicroscopy.Itwasfoundthatthesurfacecoverageandheightdistributionoftheadsorbedproteinmoleculesaredifferentfromeachother,whichispossiblytheresultofthedifferentorientationonthesurface.Itisbelievedthatthewildtypeazurinisconnectedtogoldsurfacebythedisulphidebridge;whilethemutant,K27C,mightbethroughthethiolgroupsofthecysteineresiduesontheirsurface.

简介：Thispresentstudyinvestigatedtheabilityofvarioussoyproteinhydrolysates(SPHs)inbindingcalcium.ItwasdemonstratedthattheamountofCa-bounddependedgreatlyontheSPHsobtainedusingdifferentproteases,whichincluded:neutrase,flavourzyme,proteaseMandpepsin.ThemaximumlevelofCa-bound(66.9mg/g)occurredwhenproteaseMwasusedtohydrolyzesoyprotein.PeptidefragmentsexhibitinghighCa-bindingcapacityhadmolecularweightsofeither14.4or8-9kDa.ThelevelofCa-boundincreasedlinearlywiththeincrementofcarboxylcontentinSPHs,andfurtherdeamidationonSPHsfromproteaseMimprovedCa-bindingofthehydrolysate.

简介：Theinteractionofbromothymolblue(BB)withhumanserumalbumin(HSA)wasstudiedbyelectrochemicaltechniquesandasensitivemethodforproteinsassaywasdeveloped.WhenBBinteractedwithHSA,thevoltammetricpeakcurrentvalueofBBdecreasedlinearlywiththeconcentrationofHSAinarangeof1.0―40.0mg/L,andthepeakpotentialshiftednegatively.Basedontheresults,asensitiveassaymethodforproteins,suchasHSA,bovineserumalbumin(BSA),andeggalbuminetc.wasestablished.ThismethodwasfurtherappliedtodeterminingtheHSAinhealthyhumanbloodsamples,andtheresultsarenotsignificantlydifferentfromthoseobtainedbytheclassicCoomassieBrilliantBlueG-250spectrophotometicmethod.Thedetectingconditionsofthismethodwereoptimizedandtheinteractionmechanismwasdiscussed.Theresultsshowthattheelectrochemicalparameters(formalpotentialE0,standardrateconstantoftheelectrodereactionks,parameterofkineticnα)ofBBhavenoobviouschangesbeforeandaftertheinteraction,whichindicatethatBBcaninteractwithHSA,forminganelectrochemicalnon-activecomplex.Theequilibriumconstant(βs)andthebindingratio(m)forthiscomplexwerecalculated.Themis4andβsis1.41×1019.Thismethodisfast,simple,highlysensitive,andhasgoodselectivity,whichcanbeusedinclinicalmeasurements.

简介：Sumoylationisanimportantproteinmodificationdiscoveredrecently.SUMO(smallubiquitin-relatedmodifier)pathwayregulatestheproteinstabilityandtranscriptionalactivitywitha12-kDasmallmolecularprotein,SUMO,ligatedtothetargetprotein.ThepurificationofSUMOproteinsisakeysteptorevealtheirfunction.ThepurposeofthisstudywastoconstructtherecombinantSUMO1geneclonedtoapGEX-4T-1vectortoexpressandpurifytheSUMO1-GSTfusionproteininEscherichiacoli.First,thefulllengthDNAsequenceofSUMO1genewasamplifiedbyPCRandwasligatedtopMD18-Tvector.ThentheSUMO1genewassubclonedtopGEX-4T-1prokaryoticexpressionvectorbetweenBamHIandXhoIsites,andtransformedinEscherichiacoliDH5αcells.Therightcolonieswereidentifiedbyrestrictiveenzymedigestionandsequencing.ThecorrectrebombinantplasmidofpGEX-4T-1-SUMO1wastransformedinEscherichiacoliBL21cellsandtheninducedbyIPTG(isopropyl-β-D-1-thiogalacto-pyranoside)toexpresstheSUMO1-GSTfusionprotein.ThehighlypurifiedSUMO1-GST(glutathioneS-transferase)fusionproteinwasobtainedbyaffinitychromatography.Finally,thepropertiesofSUMO1-GSTfusionproteinwereconfirmedbyCoomassiebrilliantbluestrainandWesternblotanalysis.TherecombinantplasmidofpGEX-4T-1-SUMO1wassuccessfullyconstructed,andSUMO1-GSTfusionproteinsweresuccessfullyexpressed.

简介：Preparationandcharacterizationofthehapten-proteinconjugatesarefundamentaltodevelopingenvironmentalimmunoassays.Asahapten,1-pyrenebutyricacid(PBA)wasconjugatedtothecarrierproteinofbovineserumalbumin(BSA)orovalbumin(OVA)byactiveestermethod.Infraredspectra(IR)showedthatPBA-BSAandPBA-OVAconjugatesweresuccessfullyprepared.Thenumberofthehaptensconjugatedtothecarrierproteinwasdeterminedbyultravioletspectra(UV)andmatrix-assistedlaserdesorptionionizationtime-of-flightmassspectrometry(MALDI-TOF-MS).ThecalculatedaveragebindingratiosofPBA/BSAandPBA/OVAwere18:1and10:1byUV,and31:1and22:1byMALDI-TOF-MS,respectively.Althoughtherewasadiscrepancybetweentheresultsdeterminedbythetwomethods,bothofthemwereusefulforthecharacterizationofthehapten-proteinconjugates.TheantibodywasproducedagainsttheantigenofPBA-BSA,andtheaffinitywastestedbythedoubleagardiffusionmethod.Theconjugatesandtheantibodycouldbeusedfordevelopingasensitiveandselectiveimmunoassayofpolycyclicaromatichydrocarbons(PAHs).

简介：新奇tetra-carboxylphenyl杯的相互作用[4]有牛的心细胞色素c(Cc)的arene(TCPC)被荧光光谱学和分子的建模方法首先调查。稳定的1:1建筑群的形成被荧光滴定监视，并且它的有约束力的常数是1.916脳107L摩尔?1。分子的建模揭示TCPC的识别机制到Cc表面，也就是说，静电的相互作用驾驶TCPC到Cc表面，并且货车derWaals相互作用面向TCPC平行到Cc劈开。

简介：Fromthepress-residueofthefreshroottuberofTrichosantheskirilowiiMaxim(Cu-curbitaceae),anewribosome-inactivatingprotein(RIP),trichobitacin,wasisolated.IthastheactivityofRNAN-glycosidaseandcaninhibitthegrowthofhumanplacentaltrophoblasticcells.Itsmolecularweightis27,228Da(ES-MS)andpI9.6.ItisasinglechainbasicRIP.Itsaminoacidcompositionwasdetermined.ItisanewRIP.Itconsistsof0.7~0.9%galactoseandmaybeaglycoprotein.ItsA’-andC-terminalaminoacidisAspandAla,respectively.ItsN-terminalpreliminaryaminoacidsequencehasbeendetermined.

简介：在这个工作，静止阶段与phosphorylcholine(PC)修改了的两性离子的离子层析(ZIC)的一种准备方法被hydrolyzing在结合的phosphorylcholine二氯化物以后获得到diol硅石更好在酸的蛋白质和基本蛋白质的同时的分离作为ZIC静止阶段ligand探索PC组的特征。结果证明同时，二种酸的蛋白质和三种基本蛋白质能完全被分开母鸡鸡蛋白色被分开并且净化并且三种鸡蛋白人部件ovalbumin，G2ovoglobulin和ovotransfemin蛋白质被一个单身者完全分开PC-ZIC上的步列，所有蛋白质的纯净上面到达了95%。PC-ZIC静止阶段成功地比以前在这份报纸报导与更好的分离能力和选择被改进。

简介：一综合(dimethylsiloxane)(PDMS)poly，有二的微芯片削尖为方便样品注射，跑的缓冲区更新和清洗的隧道拉长建议被介绍了。没有交换供应和注射十字隧道的复杂电源，样品直接通过拉长的入口尖端被介绍进分离隧道。运用缓冲区更新或隧道清洗的操作被vacuuming简化尖端的结束并且把另外的尖端放进答案小瓶。因此，没有软平版印刷术和血浆结合设备，这个制造方法罐头容易经济地被用于很分析的实验室。新奇PDMS微芯片的吸引人的表演被为蛋白质的分离使用导致激光的荧光察觉表明了。在0.04mol/L磷酸盐缓冲区的0.04%Brij35的增加(pH7.0)能在multienzyme药片减少蛋白质的粘附并且更容易做分离。electroosmotic流动(文件结束)在动态修改microchannel在311的范围展出pH独立。

简介：Thecompleteaminoacidsequenceofβ-momorcharin,aribosome-inactivatingproteinfromtheseedsofMomordicacharantiaLinn(Cucurbitaceae)hasbeendetermined.Thishasbeendonebythesequenceanalysisofpeptidesobtainedbyenzymaticdigestionwithtrypsin,chymotrypsinandS.aureusV8protease,aswellasbychemicalcleavagewithBNPS-skatole.Theproteinconsistsof249aminoacidresiduescontainingoneasparagine-linkedsugargroupattachedtothesiteofAsn51andhasacalculatedrelativemolecularmassof28,452Dawithoutadditionofthecarbohydrate.Comparisonofthissequencewiththoseoftrichosanthinandotherribosome-inactivatingproteinsfromdifferentspeciesofplantsshowsasignificanthomologywitheachother.Regardingthesimilarityoftheirbiologicalproperties,anactivedomainoftheseproteinshasbeenpredictedhere.

简介：Thelackofefficientandnon-toxicgenedelivery,preferablywithnon-viralDNAvectors,isgenerallyregardedasamajorlimitationforgenetherapy.Inthisstudy,awheathistoneH4genewasclonedfromTriticumaestivum,sequenced,modifiedandexpressedinE.coli.ThewheathistoneH4geneandreconstructedH4TLgeneencodedwheathistoneH4andarecombinantproteinof141aminoacidswithanapproximatemolecularweightof15500.GelelectrophoresismobilityshiftassaysdemonstratedthatthepurifiedproteinhadhighaffinityforDNA.Mostsignificantly,thecomplexofplasmidpEGFP/C1withH4TLwastransfectedwithincreasedefficiencyintoMCF-7,HO8910,LNCap,A549andHeLacellsinvitro.Theseresultsdemonstratethatthetargetingofnon-viralvectorstotumor-specificreceptorsprovidesacheap,simpleandhighlyefficienttoolforgenedelivery.

简介：Thepurposeofthisstudyistoexploretheexpressionofgrowth-associatedprotein(GAP-43)inspinalcordsegmentsconnectedwithinjuredsciaticnervebythetreatmentwithbrazileininmice.Unilateralsciaticnerveinterruptionandanastomosiswereperformed.Physiologicalsaline(blankgroup),highdose,middledoseandlowdoseofbrazileinwereadministratedintragastricallytohealthyadultBALB/cmiceinseparategroups.L4―6spinalsegmentsconnectedwiththesciaticnervewereharvested.Real-timePCR(Polymerasechainreaction)andWesternblotanalysiswereperformedtodetecttheexpressionofGAP-43inspinalsegments.Histologicalstainingonmyelinandtheelectrophysiologywereperformedtoexaminethesciaticnerverecovery.GAP-43wasactivatedinspinalcordL4―6connectedwithinjuredsciaticnerve.Inthesurvivaltimeof12h,24h,3d,5d,7dand14d,GAP-43expressioninthemotorneuronsofspinalcordofthehighdosegroupandthatinthemiddledosegroupweresignificantlyhigherthanthoseonthelowdoseandblankgroups.MyelininthehighdosegroupandthatinthemiddledosegroupweremorematureandthepotentialamplitudeandMNCV(motornerveconductionvelocity)inthehighandmiddledosegroupswereobviouslyhigherthanthoseinthelowdosegroupandblankgroup.BrazileinfacilitatestheexpressionofGAP-43inneuronsinspinalcordL4―6segmentsconnectedwithinjuredsciaticnerve,whichpromotesnerveregeneration.