简介：Theadsorptionofproteinonnanoparticleswasstudiedbyusingdynamiclightscatteringtomeasurethehydrodynamicsizeofbothpureproteinandnanoparticlesadsorbedwithdifferentamountsofprotein.Thethicknessoftheadsorbedproteinlayerincreasesasproteinconcentration,butdecreasesastheinitialsizeofnanoparticles.Afterproperlyscalingthethicknesswiththeinitialdiameter,weareabletofitallexperimentaldatawithasinglemastercurve.Ourexperimentalresultssuggestthattheadsorbedproteinsformamonolayeronthenanoparticlesurfaceandtheadsorbedproteinmoleculesareattachedtotheparticlesurfaceatmanypointsthroughapossiblehydrogen-bonding.Ourresultsalsoindicatethatasproteinconcentrationincreases,theoverallshapeoftheadsorbedproteinmoleculecontinuouslychangesfromaflatlayerontheparticlesurfacetoastretchedcoilextendedintowater.Duringthechange,thehydrodynamicvolumeoftheadsorbedproteinincreaseslinearlywithproteinconcentration.

简介：Inthisarticle,asphericalchitosangelcrosslinkedbyepichlorohydrinwasprepared.Itwasthenloadedwithcopperionstoproduceametalchelateaffinityadsorbentforprotein.Theuptakeofbovineserumalbumin(BSA)bytheaffinityadsorbentwasinvestigated,andtheadsorptioncapacityforBSAashighas40mg/g-wetbeadswasobserved.TheadsorptionequilibriumdatawaswellcorrelatedbytheLangmuirequation.TheadsorptionwasconsiderablyaffectedbypH.Inaddition,theamountofBSAadsorbedontothebeadsdecreasedwiththeincreasingofaqueousphaseionicstrength,soadsorbedBSAcanbedesorbedbyadjustingpHorionicstrengthofthesolution.

简介：Thehydrophilicityofsiliconehydrogelsusedassoftcornealcontactlensplaysanimportantroleinwearingcomfort.Inordertoenhancehydrophilicityandproteinresistance,siliconehydrogelmembranesweremodifiedbyatmosphericpressureglowdischargeplasma(APGDP)inducedsurfacegraftpolymerizationofN-vinylpyrrolidone(NVP)andpoly(oligoethyleneglycolmethylethermethacrylate)(PEGMA)inthispaper.XPSanalysisdemonstratedthesuccessofgraftpolymerizationofNVPandPEGMAontothesurfaceofsiliconehydrogelmembranes.Thehydrophilicityofsiliconehydrogelswascharacterizedbythemeasurementofwatercontactangle(WCA).TheresultshowedthatNVPgraftedsiliconehydrogelhastheWCAofabout68°andPEGMAgraftedsiliconehydrogelhasthelowestWCAofabout62°,whilethepristinesiliconehydrogelishydrophobicwiththeWCAofabout103°.Proteinresistanceofsiliconehydrogelswasinvestigatedbythemethodofbicinchoninicacidassayusingbovineserumalbumin(BSA)asamodel.It'sfoundthatthegraftedsiliconehydrogelhasasignificantimprovementofproteinresistance,andPEGMAgraftingismoreefficientforthereductionofproteinadsorptionthanNVPgrafting.ThesiliconehydrogelmembranesgraftedwithNVPandPEGMAaregoodcandidatesofsoftcornealcontactlenses.

简介：新pH应答的糖类hydrogels被设计并且准备了使用curdlan衍生物(curdlan-Boc-histidine，杂种狗--他的)。杂种狗--他的hydrogels拥有了高度多孔的结构。杂种狗的胀大的比率--他与Boc-histidine的替换的度增加的hydrogels组织。并且0.5mol/LNaCl的增加挑起了杂种狗的胀大的比率的锋利的减小--他的hydrogels。牛的浆液白朊(BSA)能高效地被包含进杂种狗--他的hydrogels。而且，在从杂种狗的不同pH价值的BSA的版本侧面--他的hydrogels是显著地不同的。这些hydrogels在cytotoxicity试金显示出好biocompatibility。杂种狗--他的hydrogels具有在象蛋白质交货系统那样的生物医学的应用的大潜力。

简介：一系列-cyclodextrin-conjugated4手臂poly(乙烯乙二醇)-poly(lactide-co-glycolide)(4手臂PEG-PLGA)共聚物被D，L减水乳酸和glycolide的戒指洞聚合作为开始者用4手臂木钉综合，然后结合了与单音(6-ethylenediamine-6-deoxy)--cyclodextrin(CDen)或衔接ethylenediamino的二度--CD(BCDen)。共聚物的化学结构被1H-NMR和FTIR光谱学。-CD-conjugatedPEG-PLGA由于CD和牛的浆液白朊(BSA)包括complexation的形成形成了稳定的反向的微粒，它能在器官的溶剂答应BSA改进封装效率。而且，我们表明了一条一步舞途径构造用这些的包含蛋白质的电影颠倒的macroporous微粒。有订的毛孔数组的电影直接从反向的微粒被准备。有趣地，蛋白质完全除了毛孔位于整个矩阵。