Images of Azurin Protein Studied by AFM

(整期优先)网络出版时间:2004-04-14
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Azurins,awildtypeandageneticallymutantK27alteredone,wereimmobilizedonannealedgoldsurfaceandinvestigatedbymeansofatomicforcemicroscopy.Itwasfoundthatthesurfacecoverageandheightdistributionoftheadsorbedproteinmoleculesaredifferentfromeachother,whichispossiblytheresultofthedifferentorientationonthesurface.Itisbelievedthatthewildtypeazurinisconnectedtogoldsurfacebythedisulphidebridge;whilethemutant,K27C,mightbethroughthethiolgroupsofthecysteineresiduesontheirsurface.