简介：与朊酶的高产量的酵母紧张10在Qingdao附近从盐场的沉积被孤立，中国。朊酶在氢指数举办了最高的活动9.0和45℃。紧张10的最大的碱的朊酶生产的最佳的媒介是2.5g在有起始的氢指数的100mL海水的可溶的淀粉和2.0gNaNO_36.0。为最大的朊酶生产的最佳的耕作条件是温度24.5℃，通风率8.0Lmin~(-1)和焦虑速度150rmin~(-1)。在最佳的条件下面，623.1Umg~(碱的朊酶的-1)蛋白质在发酵的30h以内在文化被到达。

简介：AIM:Toinvestigatetheabilityofproteaseinhibitorstomodulatehistaminereleasefromhumancolonmastcells.METHODS:Enzymaticallydispersedcellsfromhumancolonwerechallengedwithanti-IgEorcalciumionophoreA23187intheabsenceorpresenceoftryptaseandchymaseinhibitors,andhistaminereleasewasdetermined.RESULTS:IgEdependenthistaminereleasefromcolonmastceilswasinhibitedbyuptoapproximately37%,26%and36.8%bychymaseinhibitorsZ-Ile-Glu-Pro-Phe-CO2Me(ZIGPFM),N-TosyI-L-phenylalanyl-chloromethylketone(TPCK),andC~l-antitrypsin,respectively.Similarly,inhibitorsoftryptaseleupeptin,N-tosyI-L-lysinechloromethylketone(TLCK),lactoferrinandprotaminewerealsoabletoinhibitanti-IgEinducedhistaminereleasebyamaximumofsome48%,37%,40%and34%,respectively.Preincubationoftheseinhibitorswithcellsfor20rainbeforechallengedwithanti-IgEhadsmalleffectontheinhibitoryactionsoftheseinhibitorsoncolonmastcells.Aspecificinhibitorofaminopeptidaseamastatinhadnoeffectonanti-IgEinducedhistaminerelease.Thesignificantinhibitionofcalciumionophoreinducedhistaminereleasewasalsoobservedwiththeinhibitorsoftryptaseandchymaseexamined.Apartfromleupeptinandprotamine,theinhibitorstestedbythemselvesdidnotstimulatecolonmastcells.CONCLUSION:ItwasdemonstratedthatbothtryptaseandchymaseinhibitorscouldinhibitIgEdependentandcalciumionophoreinducedhistaminereleasefromdispersedcolonmastcellsinaconcentrationdependentofmanner,whichsuggestthattheyarelikelytobedevelopedasanovelclassofanti-inflammatorydrugstotreatchronicofcolitisinman.

简介：抽象昆虫由被不变的微生物引起的表面分子的识别激活的一个有效天生的免疫系统保护自己免于微生物引起的感染。在水果苍蝇果蝇melanogaster，细菌的存在在主人与抗菌剂肽的表示被联系免疫者能干的纸巾。主人受体检测感染并且中继信号装适当有免疫力的反应。在果蝇像血球的l(2)有Pefabloc的mbn房间感染前处理，一个通常使用的丝氨酸朊酶禁止者，导致了二主要效果：它响应现场克否定的细菌和细菌的表面分子(peptidoglycans污染的粗略的lipopolysaccharide)堵住了抗菌剂肽Diptericin的表示，它导致了词法变化。

简介：Toclonethegenecodingtheimmunodominantregioninthechlamydialprotease-likeactivityfactor(CPAF)fromChlamydophilapneumoniae,toanalyzeimmunocompetenceoftheexpressedprotein,andtoevaluateitsvalueinserodiagnosis,theCPAFimmunodominantregiongenewasamplified,ligatedintoapGEX6p-2vector,andthentheexpressedrecombinantproteinwaspurifiedwithglutathioneS-transferase(GST)agarosegelFFafterrenaturation,thenidentifiedbySDS-PAGEandWesternblot.AnewindirectELISAwasdevelopedwiththepurifiedproteinascoatingantigen.TheimmunogenicityoftherecombinantproteinwasevaluatedbyimmunizationtoNewZealandrabbits,anditsimmunoreactivitywasanalyzedbyreactingwithanti-C,pneumoniaeantibody.300clinicalserasampleswererespectivelyde-tectedbymicroimmunofluorescence(MIF)asreferencemethodandtheindirectELISA,andthediffer-encebetweenthetwomethodswasanalyzed.Cross-reactivityagainstChlamydiatrachomatiswasinvesti-gatedwiththeindirectELISAtodetectanti-C,trachomatispositiveantisera.Theresultsindicatedthata51.3kDarecombinantproteinwasobtained.Westernblotassayprovedthattherecombinantproteincouldmerelyspecificallyreactwithhumananti-C.pneumoniaeantisera.ThetitersofthespecificIgGan-tibodiesintheimmunizedNewZealandrabbitswereabove1:16000.Anti-C.pneumoniaeIgGpositiveandnegativereferencesereweredetectedwiththeindirectELISA,andtheconcordancerateofnegativeandpositiveresultswereboth100%(40/40).ThesensitivityandspecificityoftheindirectELISAincomparisonwithMIFwere93.8%(45/48)and100%(252/252)separatelybydetecting300clinicalserasamples,andtheconcordanceratebetweenthetwomethodswas99.0%.NocrossreactionagainstC.trachomatiswasfoundwiththeindirectELISAtodetectanti-C,trachomatispositiveantisera.Incon-clusion,thepreparedrecombinantproteinoftheCPAFimmunodominantregionshowsexcellentimmuno-competenceandcanbeusedtodevelopanewindirect

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