简介:[摘要 ]目的:为了提高气阴两虚型 2型糖尿病治疗效果,观察采用益气养阴方治疗的价值。方法:通过随机数字表法从 2017年 3月至 2018年 3月来我院治疗的气阴两虚型 2型糖尿病患者中选取 60例作为研究对象并通过抽签方式将其平均分组,其中单纯予以 2型糖尿病常规方案治疗的 30例患者设为对照组,在对照组治疗方案基础上予以益气养阴方治疗的 30例患者设为观察组,对比两组间疗效的差异。结果:研究数据显示,治疗后观察组和对照组的症状积分分别为( 5.38±2.18)和( 8.72±2.45),和对照组相比观察组患者的症状积分明显降低( P<0.05);观察组和对照组在改善胰岛素抵抗方面的总有效率分别为 18( 60.00%)和 10( 33.33%),和对照组相比观察组的总有效率明显增加( P<0.05)。结论:气阴两虚型 2型糖尿病患者接受益气养阴方治疗,既改善了患者机体的胰岛素抵抗,又减轻了患者的临床症状,效果显著,值得推广。
简介:Tosupportthescientificbasisforrapididentificationofpathogenicbacteriaandotherstud-ies,thesequencesofhsp60geneinmajor34speciesof16genusofpathogenicbacteriaweresearchoutinGenBankandaproperpairofuniversaldegenerateprimerwasdesignedbymeansofthemolecu-larbiologicalsoftwawePrimer5.0andOligo6.0.ThisprimerwasthenusedinthePCRamplification,andthehsp60genefragmentsoftheselectedpathogenicbacteriacouldbeamplifiedusingthisdegener-ateprimer.Bywayofbioinformationalanalysis,theconservation,variationandtheinterspeciesphylo-geneticrelationsofthehsp60genesequencewereanalysed.Fromtheresultsofthecomparativestudyonsequences,itwasdemonstratedthatthehsp60genewascharacterizedbyconservationandvaria-tion,inwhichtheconservedandmutantregionsco-existedandseparatelydistributedwithmanysmallmutantregionsdistributedamongtheconservedregions,justlikethemosaic.Thephylogenetictreeamongdifferentpathogenicbacteriadrawnfromthehsp60geneanalysiswasprovedtobeconsistentwiththosefrom16SrRNAand23SrRNA.Itisconcludedthatthesequencedistributionofhsp60genewouldprovideasolidbasisfortherapididentificationofpathogenicbacteriaandthedevelopmentofadiagnosticmicroarray.
简介:【摘要】 目的:探究手术治疗老年胆囊炎胆石症的临床治疗效果。 方法:选取 2016 年 1月 -2019 年 1月我院收治的 120例老年胆囊炎胆结石患者作为此次的研究对象,随机分为两组,每组患者 60例,分为实验组和对照组。对照组患者采用抗生素保守疗法,实验组患者采用手术治疗,对比两组患者的临床治疗结果。 结果:实验组总有效 58例,无效 2例,再次住院患者 1例,患者治疗过程中无 并发症,治疗总有效率为 96.66%;对照组中总有效 26例,无效 34例,再次住院患者 14例,并发症患者 2例,复发 3例,治疗总有效率为 43.33%。 结论:研究证明,外科治疗老年胆囊炎胆石症患者效果好,并发症少,复发概率低,安全有效。
简介:InordertorevealvariationandrevolutionofNPgenesofhumanavianH_5N_1influenzavirusstrains,theNPgeneofahumanavianH_5N_1influenzavirusstraininGuangdongwassequencedandtheglobalNPgenesofstrainswereretrieved.ThesequenceswereanalyzedbyDNAStar5.0,andtheevolu-tionaryspeedwasstudiedwithreferencetotheepidemiologicaldata.ItwasfoundthatNPgenesof45strainsduring1997-2006werehomologicallyclassifiedintothreegroups:strainsin1997-1998,strainsin2004-2005andstrainsfrom2003to2006.Therewere35substitutionsinNPsinallstrainsaccountingforaratioof7.03%(35/498).Anadditionalglycoproteindomain(NGT_(430-432))wasfoundinNPgenesinthestrainsof2003-2006,themutationofN_(370)SinGD-01-06resultedinoccurrenceofonemoreglyco-proteindomain(NES_(368-370)).Inthesynonymousvariation,K_svaluesinNPwere2.03×10~(-5)-2.55×10~(-5)Nt/dandK_avaluesinNPwere1.58×10(-6)-3.10×10~(-6)Nt/d.Theredidn′texistobviouslyselectivepres-sure.Anadditionalglycoproteindomainineverystrainof2003-2006andonemoreinstrainGD-01-06mightchangetheantigenicityofhumanavianH_6N_1influenzavirus.ThevariationonhumanavianH_5N_1influenzastrainsoccurredfrequentlyinthenaturalworld,whichwouldresultinhighprobabilityofhu-man-humantransmissionalongwiththenaturalevolutionofthevirus.
简介:【摘要】 目的 总结 60例省属离休干部送医送药居家康复护理的护理过程,方法和疗效。 探讨 送医送药居家康复护理符合我国居家养老的模式, 适合我国的老年人养老 。 2018年 6 月 -2019年 10月我院社区康复门诊收治了 60例省属离休干部,在药物治疗的基础上予以专科护理,康复护理及心理护理 , 结合营养指导,辅助器具的使用等方面的护理措施。结果 经过系统的上门治疗及精心的护理后, 44例老人无住院治疗, 10例老人需中途转院治疗,病情稳定再次转入我科; 6例老人转外院住院,因年龄大,器官衰竭而离世。讨论 送医送药居家康复护理适合我国的老年人养老,专业性, 高效率,低成本,能 提高晚年的生活幸福指数。
简介:摘要:目前,肥胖型2型糖尿病患者日益增多,本文将从病因病机、医案举偶、药物分析、经验总结四方面进行论述,为中医药治疗糖尿病合并肥胖提供临床经验。
简介:ToconstructandexpressthefusionproteinStx2B-IntiminC300ofEHEC0157:H7,andtofurtherinvestigateitsimmunoprophyiacticpotential,thegeneofStx2B(stx2b)fromEHEC0157:H7chromosomewasclonedintopMD18-Tvector.Thereafter,theamplifiedgenewasclonedintoprokaryoticexpressionplasmidpET-28a(+)-eaeC300,whichwasconstructedpreviously.TherecombinantpasmidpET-28a(+)-stx2b-eaeC300wastransformedintoE.coliBL21(DE3).Afterinducement,theproteinStx2B-IntiminC300wassuccessfullyexpressedandanalyzedwithsodiumdodecylsulfatepolyacrylamidegelelectrophoresis(SDS-PAGE),WesternblottingandN-terminalaminoacidresidualsequencing.Toevaluateitsimmunoprophyiacticpotential,itwasprimarilypurifiedbyion-exchangechromatographyandinjectedinto30BALB/cmicewithAl(OH)3inthesubscapularregion.Tendaysafterthelastboostervaccination,20micewereattackedwithEHEC0157:H7lysateandtheprotectiveefficacywasobserved.Inthepresentstudy,thegeneofStx2B-IntiminC300wassuccessfullyclonedintopET-28a(+)vector.TheresultsofSDS-PAGEandWesternblottingassayshowedthatthefusionproteinwassuccessfullyexpressedintheinclusionbodyform,accountingfor25%oftotalexpressionproducts,anditsmolecularweightwasabout43kDa.TheresultoftheN-terminalaminoacidresidualsequencingshowedthatitwasidenticaltothatofthemoleculardesigned.Thepuritywasabout75%afterprimarypurification.AnimaltestsrevealedthatthefusionproteinStx2B-IntiminC300haselicitedhightiterofprotectiveantibodyrelatively.TheseresultsdemonstratethatthefusionproteinStx2B-IntiminC300issuccessfullyexpressedinprokaryoticexpressionsystemandshowscertainimmunoprophyiacticpotential.