简介：Objective:Todetecttheconcentrationofmonocytechemoattractantprotein-1(MCP-1)intheserumofpatientswithincompletespinalcordinjuryandevaluateitsrelationwiththepathologicclassificationofthespinalcordinjury.Methods:MCP-1concentrationintheserumofpatientswithincompletespinalcordinjury(iSCI),singlespinecompressionandhealthysubjectsweredetectedbyELISA,respectivelyinthepresentstudyandthemagneticresonanceimagingdataofthesepatientswerestudiedatthesametimeonablindbase.Results:SerumlevelofMCP-1iniSCIpatientswas428pg/ml±11pg/mlbyELISA,whichwashigherthanboththatofthepatientswithsinglespinecompressionandofcontrols,withtheconcentrationof184pg/ml±21pg/mland124pg/ml±15pg/ml,respectively.Therewassignificantdifferencebetweenanytwogroups(P<0.01).iSCIpatientswithnormalMRIshowedalowerserumlevelofMCP-1as312pg/ml±30pg/ml.Pathologicalclassificationofspinalcordedemaandhematomacorrespondedto390pg/ml±16pg/mland508pg/ml±24pg/mlintheconcentrationofMCP-1.Conclusions:MCP-1mayinducesecondaryinflammatoryresponsebyrecruitinginflammatorycellstotheinjurysiteandthusaffecttheprognosisofspinalcordinjury.

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简介：Brassinosteroid(BR)andgibberellin(GA)aretwogroupsofplantgrowthregulatorsessentialfornormalplantgrowthanddevelopment.TogaininsightintothemolecularmechanismbywhichBRandGAregulatethegrowthanddevelopmentofplants,especiallythemonocotplantrice,itisnecessarytoidentifyandanalyzemoregenesandproteinsthatareregulatedbythem.Withtheavailabilityofdraftsequencesoftwomajortypes,japonicaandindicarice,ithasbecomepossibletoanalyzeexpressionchangesofgenesandproteinsatgenomescale.Inthisreview,wesummarizericefunctionalgenomicresearchbyusingmicroarrayandproteomicapproachesandourrecentresearchresultsfocusingonthecomparisonofcDNAmicroarrayandproteomicanalysesofBR-andGA-regulatedgeneandproteinexpressioninrice.WebelieveourfindingshaveimportantimplicationsforunderstandingthemechanismbywhichBRandGAregulatethegrowthanddevelopmentofrice.

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简介：Whitespotsyndromevirus(WSSV)isoneofthemajorshrimppathogenscausinglargeeconomiclossestoshrimpfarming.Inanattempttoidentifytheenvelopeproteinsinvolvedinthevirusinfection,purifiedWSSVvirionsweremixedwiththreeantiseraagainstWSSVenvelopeproteins(VP39,VP124andVP187),individually.Andthentheywereinjectedintramuscularlyintocrayfish(Procambarusclarkii)toconductinvivoneutralizationassays.TheresultsshowedthatforgroupsinjectedwithvirionsonlyandgroupsinjectedwiththemixtureofvirionsandantiserumagainstVP124,thecrayfishmortalitieswere100%and60%onthe8thdaypostinfection,individually.ThevirusinfectioncouldbedelayedorneutralizedbyantibodyagainsttheenvelopeproteinVP124.QuantitativePCRwasusedtofurtherinvestigatetheinfluenceofthreeantiseradescribedaboveonthevirusinfection.TheresultsshowedthattheantiserumagainstVP124couldrestrainthepropagationofWSSVincrayfish.AlloftheresultssuggestedthattheviralenvelopeproteinVP124playedaroleinWSSVinfection.

简介：Thereversetranscriptase(RT)proteinofhepatitisBvirus(HBV)hasbeensuccessfullyexpressedbyrecombinanttechnologyinEschericahiacoli(E.coli).Inthisstudyweaimedtodevelopasemi-quantitativeassayforthestudyofHBVRTproteinusingthissystem.CompleteHBVpolymerasegenefromawildtypevirus(rt306P)andthepolymerasegenefromamutant,withrt306Psubstitutedbyserine(rtP306S)wereseparatelyfusedtothemaltosebindingprotein(MBP)geneandexpressedinE.colirespectively.TheexpressionlevelsofHBVpolymerasegenesfromthewildtypevirusanditscounterpartmutantatrt306werecompared.Whentheseproteinsweresemi-quantifiedbyWesternblottingusingrabbitanti-TPserum,thertP306SmutantshoweddecreasedexpressionofMBP-HBVpolymerase.Bythismethod,wehaveshownthattheexpressionlevelofHBVRTcouldbeaffectedbysubstitutionsinitsaminoacidsequences,andthismethodcouldbeusedtostudythecharacteristicsofHBVRTprotein.

简介：象bystin一样(BYSL)基因以前被描绘为参予早胚胎培植的房间粘附编码附件蛋白质。它也涉及40Sribosomal子单元生物的续生说并且被发现在很快种胚胎和癌症房间线被表示。以便在癌症房间增长和生长探索BYSL的角色，我们把hepatocellular癌(HCC)用作一个模型。这里，我们报导BYSL在vitro并且在vivo为HCC房间生长是关键的。在人的HCC标本的BYSLmRNA和蛋白质的表示层次显著地与在邻近的非癌的织物看见的那些相比被增加。在vitro，由短发卡RNA的BYSL的抑制减少了HCC房间增长，导致了apoptosis并且部分在G2/M阶段逮捕了房间周期。在vivo，与BYSLsiRNA对待的HCC房间没能在下的培植以后在裸体老鼠形成肿瘤。为了为BYSL导致RNAi的细胞生长决定细胞的基础，逮捕，BYSLsubcellular本地化在有丝分裂并且分裂期间HepG2细胞被检验。BYSL在nucleologenesis期间在多重阶段是在场的，在导出核的foci(NDF)包括，perichromosomal区域和prenucleolar身体(PNB)在有丝分裂期间。BYSL弄空显著地压制了NDF和PNB形成，并且在有丝分裂以后破坏了nucleoli汇编，导致增加的apoptosis和到浆液饥饿的HCC房间的减少的忍耐。一起拿，我们的研究显示那upregulatedBYSL表情在hepatocarcinogenesis起一个作用。

简介：AIMTo与类型2糖尿病mellitus(T2DM)在中国病人的一个队调查在C反应的蛋白质(CRP)和糖尿病的retinopathy(医生)之间的关系基于.METHODSCommunity的观察的队学习。有1131个参加者，在城市的北京在Desheng社区从2009年11月招募到2011年9月。病人们诊断了T2DM被招募并且经历由一张问询表，眼睛、人体测量的检查和实验室调查组成的标准化评估。医生的存在和严厉由七个领域被估计30°；渲染宫底相片。题目然后没有医生，任何医生，或威胁视觉的克尔普博士被分类进组与T2DM从1007个病人全部的学习subjects.RESULTSA的浆液被分析为分析被包括，包括408(40.5%)男人并且599(59.5%)女人。中部的CRP水平为男人是为女人和1.1mg/L的1.5mg/L(P=0.004，或0.37，95%CI0.18-0.74)。在为可能的covariates调整以后，CRP的高水平与任何医生的更低的流行被联系(P=0.02，或0.55，95%CI0.35-0.89)，然而并非与威胁视觉的医生联系了(P=0.62，或0.78，95%CI0.28-2.14)。在由性的层化以后，在CRP和医生之间的反的协会被发现在男人统计上重要(P=0.006，或0.35，95%CI0.16-0.73)，然而并非在女人(P=0.58，或0.88，95%CI0.29-1.16)与T2DM从一张中国人口拉的.CONCLUSIONThe数据建议那增加的CRP层次可以相反地与医生的开发被联系。

简介：Thethree-dimensional(3D)structurepredictionofproteinsisanimportanttaskinbioinformatics.Findingenergyfunctionsthatcanbetterrepresentresidue-residueandresidue-solventinteractionsisacrucialwaytoimprovethepredictionaccuracy.Thewidelyusedcontactenergyfunctionsmostlyonlyconsiderthecontactfrequencybetweendifferenttypesofresidues;however,wefindthatthecontactfrequencyalsorelatestotheresiduehydrophobicenvironment.Accordingly,wepresentanimprovedcontactenergyfunctiontointegratethetwofactors,whichcanreflecttheinfluenceofhydrophobicinteractiononthestabilizationofprotein3Dstructuremoreeffectively.Furthermore,afoldrecognition(threading)approachbasedonthisenergyfunctionisdeveloped.Thetestingresultsobtainedwith20randomlyselectedproteinsdemonstratethat,comparedwithcommoncontactenergyfunctions,theproposedenergyfunctioncanimprovetheaccuracyofthefoldtemplatepredictionfrom20%to50%,andcanalsoimprovetheaccuracyofthesequence-templatealignmentfrom35%to65%.

简介：真核细胞的房间的最惹人注目的形态特征是各种各样的围住膜的分隔空间的存在。包括细胞器和短暂运输中介，这些分隔空间不是静态的。更确切地说，蛋白质和膜的动态交换被需要维持细胞的动态平衡。膜动员的最戏剧的例子之一在macroautophagy的过程期间被看见。Macroautophagy是为长寿蛋白质和细胞器的降级的主要细胞的小径。响应环境暗示，例如饥饿或应力的另外的类型，房间生产唯一的膜结构，phagophore.Thephagophore扣押它形成一个双膜cytosolic泡的细胞质，anautophagosome。在结束之后，autophagosome与溶酶体熔化或在酵母的一个液泡，它交付水疗院放射激光与它的货物，和产生大分子一起毁坏内部autophagosome膜回来被释放进cytosol因为reuse.Autophagy因此是正在骑车过程，允许房间熬过滋养的限制的时期；然而，它有一个更宽的生理的角色，参予开发和老化，并且另外在对病原体侵略，癌症和某些neurodegenerative的保护疾病。在许多情况中，autophagy的角色通过autophagy相关的蛋白质的研究被识别，Atg6/Beclin1。这蛋白质是类脂化合物kinase建筑群的部分，并且最近的研究建议它在协调autophagy并且在反对apoptosis的细胞的死亡过程的thecytoprotective功能起一个中央作用。这里，我们总结我们Atg6/Beclin的当前的知识1在在细胞的不同模型有机体和它的唯一的功能。

简介：Objective:ToinvestigatethemembranelocalizationfunctionoftheCX26proteinwhenits86thaminoacidisThr,SerorArg,anditsrelationstodeafness.Methods:CX26-GFPproteinwitheitherThr,SerorArgasthe86thaminoacidwasexpressedinmouseSGNcellsviatheGFPfusiontypelentivirusexpressionsystem.Themembranelocalizationofthefusionproteinwasobservedunderafluorescencemicroscope.Results:ThemutatedproteinofCX26T86Swaslocalizedtocellmembraneandformgapconjunctionstructures,showingnodifferencetothewildtypeCX26protein(withThrasthe86thaminoacid).However,thegapconjunctionstructuredisappearedwhenthemutationwasCX26T86A.Conclusion:TheseresultsindicatethattheCX26T86Rmutationmaybeacauseofhearingloss,butCX26T86Sasanon-pathogenicpolymorphismmutationdoesnotaffectfunctionsoftheCX26protein.Theresultsareinaccordancewiththeresultsofclinicalscreening.

简介：UnderstandingthecouplingspecificitybetweenGprotein-coupledreceptors(GPCRs)andspecificclassesofGproteinsisimportantforfurtherelucidationofreceptorfunctionswithinacell.IncreasinginformationonGPCRsequencesandtheGproteinfamilywouldfacilitatepredictionofthecouplingpropertiesofGPCRs.Inthisstudy,wedescribeanovelapproachforpredictingthecouplingspecificitybetweenGPCRsandGproteins.ThismethodusesnotonlyGPCRsequencesbutalsothefunctionalknowledgegeneratedbynaturallanguagepro-cessing,andcanachieve92.2%predictionaccuracybyusingtheC4.5algorithm.Furthermore,rulesrelatedtoGPCR-Gproteincouplingaregenerated.Thecom-binationofsequenceanalysisandtextminingimprovesthepredictionaccuracyforGPCR-Gproteincouplingspecificity,andalsoprovidescluesforunderstandingGPCRsignaling.

简介：预言潜水艇的能力从它的顺序的蛋白质的细胞的本地化是很重要的，它关于蛋白质的功能提供信息。我们在场一个计算工具，PredSL，它利用神经网络，Markov链，隐藏的Markov建模的侧面，和得分为潜水艇的预言的矩阵在从N终端氨基酸顺序的真核细胞的房间的蛋白质的细胞的本地化。它试图分类蛋白质进五个组：叶绿体，thylakoid，线粒体，能分泌的小径，和“其它”。当在一个五倍的交叉验证过程测试了时，PredSL分别地为植物和非植物数据集表明86.7%和87.1%全面精确性。与TargetP，迄今为止是最广泛地使用的方法，和LumenP相比，PredSL的结果在大多数情况中是可比较的。当在Saccharomycescerevisiae染色体的试验性地验证的蛋白质上测试了时，PredSL可比较地表现如果比为一样的任务的任何可得到的算法不好。而且，PredSL是为这些的预言有能力的唯一的方法代替作为通过URL:http://bioinformatics.biol.uoa.gr/PredSL/的独立应用可得到的细胞的本地化。

简介：AcomputationalsystemforthepredictionandclassificationofhumanG-proteincoupledreceptors(GPCRs)hasbeendevelopedbasedonthesupportvectormachine(SVM)methodandproteinsequenceinformation.ThefeaturevectorsusedtodeveloptheSVMpredictionmodelsconsistofstatisticallysignificantfeaturesselectedfromsingleaminoacid,dipeptide,andtripeptidecompositionsofproteinsequences.Furthermore,thelengthdistributiondifferencebetweenGPCRsandnon-GPCRshasalsobeenexploitedtoimprovethepredictionperformance.ThetestingresultswithannotatedhumanproteinsequencesdemonstratethatthissystemcangetgoodperformanceforbothpredictionandclassificationofhumanGPCRs.

简介：translational以后修正对蛋白质稳定性并且到蛋白质活动的调整中央。蛋白质修正的各种各样的类型例如phosphorylation，methylation，acetylation，myristoylation，glycosylation，和ubiquitination，被报导了。在他们之中，ubiquitination把自己与其它区分开来因为大多数ubiquitinated蛋白质为降级被指向到26Sproteasome。ubiquitin/26Sproteasome系统在房间组成主要蛋白质降级小径。在最近的年里，在众多的真核细胞的细胞的函数的控制的ubiquitination机械的重要性逐渐地被欣赏了。增加E3ubiquitinligases和他们的底层的数字，包括许多必要细胞的管理者被识别了。过去的几年里的研究表明了ubiquitination系统为大量植物是重要的发展过程和回答到不能生活、关於生命的压力。这评论从在植物微生物相互作用起重要作用的植物和病原体在联系ubiquitination的蛋白质的功能的分析讨论最近的进展。

简介：ThisstudysoughttoidentifydifferentiallyexpressedproteinsinSH-SY5Ycellstreatedwithvalproicacid,usingtwo-dimensionaldifferencegelelectrophoresisanalysis.Threeproteinswereunambi-guouslyidentified:theeukaryotictranslationinitiationfactor4Aisoform1andATP6V1B2proteinweredownregulated,whiletheheterogeneousnuclearribonucleoproteinKwasupregulated.Moreover,allthreeproteinsareassociatedwithalteredexpressionduetooxidativestress.Ma-trix-assistedlaserdesorption/ionization-timeofflightmassspectrometryandproteinimmunoblottingassayconfirmedthedifferentialexpressionofeukaryotictranslationinitiationfactor4Aisoform1.Theresultsindicatethatvalproicacidexertsanantioxidationeffectbyregulatingtheexpressionofeukaryotictranslationinitiationfactor4Aisoform1.

简介：CREB有约束力的蛋白质(CBP)和它的相当或相同事物p300是涉及细胞的活动的一个宽数组的各种各样的顺序特定的抄写因素的transcriptionalco使活跃之物，例如脱氧核糖核酸修理，房间生长，区别和apoptosis。Severalstudies建议了CBP和p300可能被看作瘤压制ors，与他们是的突出的角色响应各种各样的刺激的不同基因表示模式的跨coupling。他们主要经由乙酰化施加行动嘘和另外的规章的蛋白质(例如p53)。在CBP/p300功能的主要悖论是他们似乎能够贡献各种各样的反对细胞的进程。呼吸上皮tumorigenesis代表一个范围的多步累积的一个复杂过程基因并且epigenetic错误。通过激活和压抑的建筑群的交替的形成的Transcriptionmodulation是这些精神错乱的最终的收敛点，并且CBP/p300在这相互影响代表关键参加者。因此，他们的分子的行动和相互作用的照明能在呼吸上皮carcinogenesis为药理学干预揭示新潜在的目标。

简介：Objective:Toexplorethecontentchangeofneurofilament(NF)proteinsubunitsintheexperimentalbraindiffuseaxonalinjury(DAI)bylateralheadrotation.Methods:Twenty-fourSpragueDawley(SD)ratswereequallydividedintothreeinjurygroups(2h,12h,and24hpostinjury)andonecontrolgroup.ThemodelsofDAIweremadeintheinjurygroupsbylateralheadrotation.westernblottingtechniquewasusedtomeasuretheconteneofNF68(akindofNFproteinsubunit)inthebrainstemtissuesamongalltheinjuredandcontrolrats.TheNF68immunohistochemicalstainingwasusedinanothersixSDratsinordertoobservethemorphologicalchangesinDAI.Results:TheNF68contentinthebrainstemtendedtodecreaseat2hpostinjury,decreasedsignificantlyat12handcontinueditsdecreaseat24h.NF56andNF52,asthebreakdownproductsofNF68,hadatendencytoincreaseat2-12haftertheinjury,andamountedtoasignificantlyhigherlevelat24h.Microscopically,therewerealotofswellingneuronalaxonsintheventralpartofthemedullaroblongateat2haftertheinjury.someaxonsweredisconnected,andaxonalretractionballsformedontheirproximalend.Conclusions:ThereisandoccurrenceofphosphorolysiswithinthebrainsteminDAIbylateralheadrotation.ThesereactionscausethebreakdownofNF68,whichresultsinthedecreaseofNF68incontent.ItsuggeststhatthebreakdownofneurofilamentproteinsubunitsisanimportantreasonforstructuraldestroyofneurofilamentsinDAI.

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