简介：AbstractObjective:Venlafaxine is a common antidepressant and its therapeutic effect varies among people with different genetic backgrounds. The aim of this study was to investigate whether single nucleotide polymorphisms (SNPs) in the SLC17A7 gene are associated with the treatment outcome of venlafaxine in a Chinese Han population with major depressive disorder.Methods:This prospective pharmacogenetic case-control study that involved genotyping of four SNPs of SLC17A7 was conducted on 175 major depressive disorder patients of Chinese Han origin, aged 18 to 65 years, participated in the study from April 2005 to September 2006. Comparisons of allele and genotype frequencies of all SNPs were performed between the responder/remission group and the nonresponder/nonremission group. This study was approved by the Institutional Ethics Committee of Sichuan University (approval No. 20151112-265).Results:The allele and genotype frequencies of the four candidate SNPs in SCL17A7 showed no significant difference between responders and nonresponders. Meanwhile, no significant difference was detected in the four investigated SLC17A7 SNPs between patients who did and did not exhibit remission. Although one of the investigated SLC17A7 variants (rs1578944) demonstrated a significant association (P=0.022) with a response to venlafaxine after 6 weeks of treatment in the survival analysis, the association was unclear after a Bonferroni multiple comparisons test was conducted.Conclusion:No significant association exists between the four candidate SNPs (rs1043558, rs1320301, rs1578944, and rs74174284) in SLC17A7 and venlafaxine treatment in the Chinese Han population.

简介：摘要胶质瘤是成人最常见的原发脑肿瘤，其本质是一种多基因异常疾病。溶质载体家族7成员11（SLC7A11）是编码胱氨酸/谷氨酸反向转运体的核心成分，其表达受转录和翻译层面的调控。SLC7A11通过调节氧化应激与铁死亡介导胶质瘤细胞增殖、侵袭和放化疗抵抗。深入研究SLC7A11将为胶质瘤的治疗提供新的理论依据和治疗靶标。

简介：Objective:Tostudythevaccinepotencyofgene-modifiedtumorcells.Methods:TheEL-4lymphomawastransducedwithrecombinantretroviruscontainingthemurineGM-CSFgeneorB7-1gene.Theeffectofgenetransductiononantitumorimmunitywasinvestigated.Results:Flowcytometryanalysisshowedthatexpressionoftheirsurfacemarkerbetweenwild-typeEL-4cellsandgenetransducedtumorcellswasthesameexceptforCD80positiveinB7-1genetransducedcells.GM-CSFgeneorB7-1genetransducedEL-4cellsresultedinremarkablelossoftumorigenicityinsyngeneticmice.ThesystemicprotectiveimmunitywasinducedagainstthechallengewithEL-4/wtcells.TherapeuticvaccinewithEL-4/GM-CSForEL/7-1cellscouldretardthegrowthofestablishedearly-stageEL-4/wttumorsignificantly,butnotretardthegrowthoflate-stageEL-4/wttumor.IrradiatedGM-CSFgenetransducedEL-4cellsshowedstrongvaccineeffectagainstEL-4cellchallenge,butirradiatedB7-1genetransducedEL-4cellsshowedweakvaccineeffect.RemarkablecooperativeantitumoreffectagainstEL-4cellchallengewasobservedwhenbothirradiatedEL-4/GM-CSFandEL-4/B7-1wereinoculatedtogether.Conclusion:GM-CSFgeneorB7-1genetransducedcombinationofthetwokindsofvaccinemayhavepotentialapplicationvalueinhumancancertreatment.

简介：Objective:ToobtainrecombinantTreponemapallidumsubsp,pallidum(TP17KD)lipoproteininlargequantitiesbyamplificationandtofurtherpurifyantigensforlaboratorydiagnosisofsyphilisanddevelopmentofasyphilisvaccine.Method:TheTppl7lipoproteingenewasamplifiedfromtheTP(strainNichols),andthenitwasrecombinatedintoaplasmidpMAL-2candclonedwithinE.coli12-TB1.ThehostbacteriacontainingrecombinantplasmidswereinducedwithIPTG.TheTpp17KDlipoproteingenewasamplifiedbyus-ingPCRandpositivecloneswerescreenedwithdoubledigestionandPCR.RecombinantplasmidsweretransformedintoE.coliandtheE.colicarryingrecombinantplasmidswereinduced.TheexpressionofTP17KDwasdetectedbysodiumdedecylsulfate-polyacrylamidegelelectrophoresis(SDS-PAGE)andimmunoblot.Results:GelstainingwithCoomassieblueG-250showedthattheinducedE.colicarryingrecombinantplasmidcouldproduce60KDfusionproteinathighlevels.Gelscanningshowedthat17KDproteinexpressioninE.coliaccountedfor10%oftotalcellularprotein.Therecombinantproteinantigenreactedwiththeseraofsyphilispatients.Conclusion:Ourstudylaysacornerstonefordevelopingnewtechniquesoflaboratorydiagnosisforsyphilisandnewvaccines.Preliminaryclinicalapplicationshowedthatthefusionproteincouldbeusedforthediagnosisofsyphilis.

简介：无

简介：Themajorstoragesubstanceinriceendospermisstarch,whichaccountsfor80%ofdrymatterweight.Inthisstudy,ricemutantflo7,selectedfromtheprogenyofNipponbare’stissueculture,displayedflouryandopaqueendosperm.Comparedwithitscorrespondingwildtype(WT)Nipponbare,themutantflo7producedlonger,narrower,thinnerandlightergrains.Thelevelsofglucose,fructoseandsucroseinthemutantflo7endospermwerehigherthanthoseintheWTendosperm,whereastheproteincontentwasnotaffected.Withrespecttobothamylosecontentandgelconsistency,themutantflo7waslowerthanWT,butitsalkalivaluewashigher.Scanningelectronmicroscopicexaminationsshowedthattheendospermofthemutantflo7containedirregular,looselypackedandcompoundstarchgranules.Geneticanalysisindicatedthatthemutantphenotypewasdeterminedbyasinglerecessivenucleargene.Theflo7locuswasmappedtoaregiononthelongarmofchromosome12,withina95.1kbintervaldefinedbythemarkersC2-11andC5-15.Thereare13openreadingframesinthemappinginterval.Transcriptionprofilingofthedevelopinggrainsshowedthatanumberofgenesinvolvedinstarchsynthesiswereaffecteddifferentlyinthemutantflo7.

简介：在这研究，我们描述从蚊子疟蚊属stephensi孤立的ribosomalproteinS7基因的部分genomic组织。开始558bp部分cDNAsequence作为长包含223bp的先锋信使rna顺序被放大intron。5''和3''结束序列用cDNA的特定的快速的扩大结束的结束(种族)被恢复聚合酶链反应。全身的cDNA顺序与能够长编码192氨基酸的一个开的读物框架长是914核苷酸有22174Da和pI的计算分子的质量的蛋白质点of9.94。蛋白质相同搜索揭示了>75%身份到另外的昆虫“sS7ribosomal蛋白质。顺序排列的分析揭示了几个高度保存的领域，其一个与原子本地化有关是人的rpS7的信号(NLS)区域。有趣地，有A的intron核苷酸顺序比较。gambiae作为与编码anduntranslated区域相比显示出保存的更小的度。象这一样，早在genomic组织和分析(EST)能在A的染色体注解帮助的cDNA/Expressedsequence标签上学习。stephensi，并且将可能以后被定序。

简介：Objective:TostudythesynergiceffectsofIL-12andB7-1transfectantonantitumorimmunityinvivo.Methods:TheretrovirusvectorencodingmIL-12andmB7-1genewastranfectedintoEL-4thymiclymphomacellsrespectively.Thecellswereusedastumorvaccineandthetherapeuticeffectwasobserved.Results:IncontrasttothemiceimmunizedwithEL-4/WtorEL-4/Neogroups,thetumorigenicityofEL-4/IL-12transfectantwasdecreased(P<0.001).TheEL-4/IL-12andEL-4/B7-1cellsirradiatedwith60CoshowedsignificantsystematicprotectiveeffectsagainsttherechallengeofEL-4/Wt.60CoirradiatedEL-4/IL-12cellsdelayedtheoccurrenceoftumorandprolongedthesurvivalperiodoftumorbearingmice.CombinationofthevaccinesofEL-4/IL-12andEL-4/B7-1resultedintheenhancedtherapeuticeffectcomparedwitheachsingletransfectantgroup(P<0.001).Conclusion:TheresultsshowedthatIL-12transducedcellscouldenhancetheantitumorimmunityofhostascancervaccine.CombinationoftheEL-4/IL-12andEL-4/B7-1transfectantcouldimproveimmunityofhostandisaprospectcancervaccine.

简介：~~

简介：摘要目的描述3～17岁儿童青少年体成分（脂肪组织、非脂肪组织）的发育特征。方法采用分层整群抽样方法，分别抽取南方和北方共计7个城市，包括长春、北京、天津、济南、上海、银川、重庆的3～17岁城市汉族儿童青少年10 867例。采用问卷调查收集研究对象基本信息，测量身高、体重，采用双能X线吸收法（DXA）测量身体脂肪组织质量、非脂肪组织质量等。计算BMI、体脂含量百分比（FMP）、脂肪质量指数（FMI）、非脂肪组织质量指数（FFMI）。采用SAS 9.4软件进行数据整理和逻辑核查，采用SPSS 20.0软件进行统计学分析。结果共收集10 867例3～17岁儿童青少年数据，其中男生5 512人（50.7%）。男生FMP在10～15岁组随年龄增长迅速下降[β＝-1.811（95%CI：-1.987～-1.635）]，15岁以后变化不明显；女生FMP在3～7岁组随年龄增长呈下降趋势[β＝-0.896（95%CI：-1.100～-0.691）]，7～12岁变化不明显，12～15岁随年龄增长增速明显[β＝0.989（95%CI：0.753～1.224）]，15岁以后变化不明显。除9岁组和10岁组以外，女生FMP高于男生（均P<0.05）。男生FFMI在3～17岁各个年龄段均明显高于女生（均P<0.05），11岁以后，女生和男生的FFMI差异变大。男生和女生的FMI随年龄变化的生长曲线有交叉，略有随年龄增长而增加的趋势[男生：β＝0.033（95%CI：0.018～0.048）；女生：β＝0.192（95%CI：0.181～0.204）]。脂肪重聚年龄肥胖组小于超重组和正常体重组；男生不同体重状态组BMI、FFMI随年龄变化情况类似；男生正常体重组FMI随年龄增长略有降低，一直保持在5 kg/m2以下，超重组[β＝0.114（95%CI：0.091～0.136）]和肥胖组[β＝0.211（95%CI：0.176～0.245）]的FMI有明显随年龄增长而增加的趋势；不同体重状态组男生FMP在10岁以后随年龄增长呈下降趋势[正常体重：β＝-0.836（95%CI：-0.924～-0.748）；超重：β＝-1.090（95%CI：-1.269～-0.910）；肥胖：β＝-1.144（95%CI：-1.321～-0.967）]；不同体重状态组女生的BMI、FFMI、FMI随年龄变化情况类似，在8岁以后呈现随年龄增长而上升的趋势[正常体重组：β＝0.174（95%CI：0.165～0.182）；超重组：β＝0.325（95%CI：0.304～0.346）；肥胖组：β＝0.447（95%CI：0.406～0.488）]；女生FMP的变化12岁以后随年龄增长呈明显增加的趋势[正常体重组：β＝0.963（95%CI：0.851～1.074）；超重组：β＝0.910（95%CI：0.695～1.125）；肥胖组：β＝0.895（95%CI：0.569～1.221）]。总体上来看，BMI和FMI的相关性很强（男生：r＝0.767；女生：r＝0.873），不同体重状态儿童青少年BMI和FMI的r不同。结论儿童脂肪组织和非脂肪组织发育特征不同，且存在性别差异。BMI生长曲线和体脂肪的发育特征不完全一致，且存在性别差异，有必要对生长发育中的儿童进行更精准的体成分评估。

简介：

简介：摘要目的对福建宁德地区的新生儿进行串联质谱筛查，分析17例疑似原发性肉碱缺乏症(primary carnitine deficiency，PCD)新生儿的血游离肉碱(C0)以及SLC22A5基因的变异，了解本地区的发病情况，并探讨C0水平与基因型的相关性。方法选取2016年9月至2021年6月在宁德市9个县(市、区)出生的148 043例新生儿为研究对象，进行血游离肉碱和酰基肉碱谱分析，对其中血C0 < 10 μmol/L或C0在10 ~ 15 μmol/L的新生儿进行SLC22A5基因检测，分析其游离肉碱水平与基因变异之间的相关性。结果共确诊17例PCD，折算新生儿患病率为1/8 707。共发现12种SLC22A5基因变异，其中热点变异为c.760C>T、c.1400C>G、c.51C>G。相比于其他位点，携带c.760C>T变异者的C0值显著偏低(P<0.01)。结论宁德地区新生儿PCD的患病率较高，需制定干预措施积极防控。携带c.760C>T变异者C0水平明显偏低，可为临床诊断提供一定的依据。

简介：利用常规观测资料和榆林多普勒雷达（CB）观测资料,分析2009年7月16—17日发生在陕北地区的大暴雨天气过程,结果表明：大暴雨发生时段,对应着低层偏东或东南气流风速增大后再减小的过程。当低层气流风速增大后再减小,中低层低空急流建立时出现强降水。暴雨区高空急流南侧强辐散形成的上升气流和中低空急流风速持续增加形成的上升气流的叠加耦合为大范围暴雨天气的产生创造了有利的上升运动条件。大暴雨出现在中尺度辐合线附近,中尺度辐合线为大暴雨的发生提供了充分的水汽和更多的不稳定能量,使水汽的上升和不稳定能量的释放集中在一个更小的区域,从而使得降水雨强比仅由高低空急流耦合形成的更大。上游西北气流侵入强迫抬升前方偏南暖湿气流促使中尺度辐合线产生,当高层西北气流侵入到低层海拔3km以下,暴雨天气结束。

简介：摘要根据1982、1992、2002和2010—2012年中国居民营养与健康状况调查7~17岁儿童的体格测量数据，1982—2012年7~17岁儿童身高和体重持续增长。城市男生、城市女生、农村男生、农村女生的身高分别为118.8~171.1、117.8~159.9、113.3~168.2、111.7~158.1 cm，体重分别为21.3~61.4、20.2~53.1、19.4~57.9、19.0~51.5 kg；城市儿童的身高和体重高于农村，除青春期外，男生的身高和体重均高于女生。与1982年相比，2012年城市男生、城市女生、农村男生、农村女生的身高平均增幅分别为8.8、6.2、12.9、10.8 cm，体重平均增幅分别为9.9、6.2、9.8、7.6 kg；城市儿童的身高和体重增幅小于农村，城乡差异缩小；男生的增幅大于女生，性别差异扩大。

简介：

简介：从古巴的蝎子Rhopalurusjunceus表示昆虫选择的神经毒素(RjAa17f)的recombinantHelicoverpaarmigeranucleopolyhedrovirus(HearNPV)被用红相应再结合系统代替UDP-glucosyltransferase基因(egt)构造。另一egt删除了控制HearNPV被把egfp基因插入到egt地点以一个类似的方法构造。一步舞病毒的生长曲线和病毒的DNA复制曲线分析证实再结合没在宿主细胞影响病毒的生长和DNA复制。在在RjAa17f-HearNPV，Egfp-HearNPV和HZ8-HearNPV之间的吸藏身体形态发生没有可辨别的差别，它被传播电子显微镜学分析证实。然而，RjAa17f-HearNPV的杀虫的活动对第三中间形态H被提高。armigera幼虫根据毒力比较上的生物鉴定。在中部的致命的剂量有戏剧的减小(56.9%)(LD50)并且另外在中部的幸存时间的减小(13.4%)(为与HZ8-HearNPV相比的recombinantRjAa17f-HearNPV的圣50)，但是当Egfp-HearNPV与HZ8-HearNPV相比时，仅仅在LD50的27.5%减小和在圣50的10.1%减小珍视。每日的食谱消费分析证明RjAa17f-HearNPV能禁止减HearNPV与egt相比喂的感染的幼虫。这些结果证明这新奇recombinantRjAa17f-HearNPV能对它的主人昆虫改进杀虫的效果，RjAa17f能是另外的recombinantbaculovirus构造的一个可观的候选人。

简介：ObjectiveThestudyistoidentifythecarrierrateofcommondeafnessmutationinChinesepregnantwomenviadetectingdeafnessgenemutationswithgenechip.MethodsThepregnantwomeninobstetricclinicwithouthearingimpairmentandhearingdisordersfamilyhistorywereselected.Theinformedconsentwassigned.PeripheralbloodwastakentoextractgenomicDNA.Applicationofgeneticdeafnessgenechipfordetecting9mutationalhotspotofthemostcommon4Chinesedeafnessgenes,namelyGJB2(35delG,176del16bp,235delC,299delAT),GJB3(C538T),SLC26A4(IVS72A>G,A2168G)andmitochondrialDNA12SrRNA(A1555G,C1494T).Furthergenetictestingwereprovidedtothespousesandnewbornsofthescreenedcarriers.ResultsPeripheralbloodof430pregnantwomenweredetected,detectionofdeafnessgenemutationcarriersin24cases(4.2%),including13casesoftheGJB2heterozygousmutation,3casesofSLC26A4heterozygousmutation,1casesofGJB3heterozygousmutation,and1caseofmitochondrial12SrRNAmutation.18spousesand17newbornstookfurthergenetictests,and6newbornsinheritedthemutationfromtheirmother.ConclusionThecommondeafnessgenesmutationhasahighcarrierrateinpregnantwomengroup,235delCandIVS7-2A>Gheterozygousmutationsarecommon.

简介：Genestellcellswhattodo,forexample,whentorepairDNAmistakesorwhentodie,andcanbeturnedonorofflikealightswitch.Knowingwhichgenesareswitchedon,orexpressed,isimportantforthetreatmentandmonitoringofdisease.Now,forthefirsttime,Researchersinthelaboratoryof

简介：Wehavereviewedthegenetherapyingastrointestinaldiseases^[1].GastriccanceriscommoninChina^(2-20),anditsearlydiagnosisandtreatmentarestilldifficultuptonow^(13-36).Theex-pressionofanexogenousgeneintroducedbygenetherapyintopa-tientswithgliomascanbemonitorednon-invasivelybypositron-emissiontornography^[4].

简介：Objective:TostudythechangesofthegeneexpressionpatternofspinalcordtissuesintheearlystageafterinjurybyDNAmicroarray(genechip).Methods:ThecontusionmodelofratspinalcordwasestablishedaccordingtoAllen'sfallingstrikemethodandthegeneexpressionpatternsofnormalandinjuredspinalcordtissueswerestudiedbygenechip.Results:Theexpressionof45geneswassignificantlychangedintheearlystageafterspinalcordinjury,inwhich22genesup-regulatedand23genesdown-regulated.Conclusions:Theexpressionofsomegeneschangessignificantlyintheearlystageafterspinalcordinjury,whichindicatesthecomplexityofsecondaryspinalcordinjury.