简介：UnderType-IIprogressivelyhybridcensoring,thispaperdiscussesstatisticalinferenceandoptimaldesignonstepstresspartiallyacceleratedlifetestforhybridsysteminpresenceofmaskeddata.ItisassumedthatthelifetimeofthecomponentinhybridsystemsfollowsindependentandidenticalmodifiedWeibulldistributions.Themaximumlikelihoodestimations(MLEs)oftheunknownparameters,accelerationfactorandreliabilityindexesarederivedbyusingtheNewton-Raphsonalgorithm.Theasymptoticvariance-covariancematrixandtheapproximateconfidenceintervalsareobtainedbasedonnormalapproximationtotheasymptoticdistributionofMLEsofmodelparameters.Moreover,twobootstrapconfidenceintervalsareconstructedbyusingtheparametricbootstrapmethod.TheoptimaltimeofchangingstresslevelsisdeterminedunderD-optimalityandA-optimalitycriteria.Finally,theMonteCarlosimulationstudyiscarriedouttoillustratetheproposedprocedures.

简介：

简介：Aco-designschemeofevent-triggeredsamplingmechanismandactivefaulttolerantcontrol(FTC)isdeveloped.Firstly,afaultdiagnosisobserverisdesignedtoestimateboththefaultandthestatesimultaneouslybyusingtheevent-triggeredsampledoutput.SomeH∞constraintsbetweentheestimationerrorsandtheevent-triggeredsamplingmechanismareestablishedtoensuretheestimationaccuracy.Then,basedontheconstraintsandtheobtainedfaultinformation,anevent-triggereddetectorandastaticfaulttolerantcontrollerareco-designedtoguaranteethestabilityofthefaultysystemandtoreducethesensorcommunicationcost.Furthermore,theproblemoftheeventdetectoranddynamicFTCco-designisalsoinvestigated.Simulationresultsofanunstablebatchreactorarefinallyprovidedtoillustratetheeffectivenessoftheproposedmethod.

简介：Fluorescentproteins(FPs)withemissionwavelengthsinthefar-redandinfraredregionsofthespectrumprovidepowerfultoolsfordeep-tissueandsuper-resolutionimaging.Thedevelopmentofred-shiftedFPshasevokedwidespreadinterestandcontinuousengineeringefforts.Inthisarticle,basedonacomputationaldesignandgeneticcodeexpansion,wereportarationalapproachtosignificantlyexpandandred-shiftthechromophoreofgreenfluorescentprotein(GFP).WeappliedcomputationalcalculationstopredicttheexcitationandemissionwavelengthsofaFPchromophoreharboringunnaturalaminoacids(UAA)andidentifyinsilicoanappropriateUAA,2-amino-3-(6-hydroxynaphthalen-2-yl)propanoicacid(naphthol-Ala).OurmethodologyallowedustoformulateaGFPvariant(cpsfGFP-66-Naphthol-Ala)withred-shiftedabsorbanceandemissionspectralmaximaexceeding60and130nm,respectively,comparedtothoseofGFP.TheGFPchromophoreisformedthroughautocatalyticpost-translationalmodificationtogenerateaplanar4-(p-hydroxybenzylidene)-5-imidazolinonechromophore.WesolvedthecrystalstructureofcpsfGFP-66-naphthol-Alaat1.3■resolutionanddemonstratedtheformationofamuchlargerconjugatedn-systemwhenthephenolgroupisreplacedbynaphthol.Theseresultsexplainthesignificantred-shiftingoftheexcitationandemissionspectraofcpsfGFP-66-naphthol-Ala.

简介：

简介：通过在艺术设计类专业学生的设计思维的教学创新实践,从课程的教学目标、教学内容、教学方法、教学成果四个方面探究创造性思维课程的教学设计,在团队协作和学科融合中培养学生的创新意识,提高学生的创造性思维应用能力。

简介：