简介：Astructuralgene(750bp),whichcodesforatypeIribosomeinactivatingprotein,trichosanthin,hasbeendesignedaccordingtothecodonusageofhighlyexpressedgeneinE.coliandchemicallysynthesized.Inthesynthesizedgene,twenty-sevenuniquerestrictionsiteswereevenlydispersedwithanaveragedistancebetweentwoadjacentsiteslessthan50bptofacilitateasystematicinvestigationonstructure-functionalrelationshipofthisproteinbysite-directedmuta-genesis.Tosynthesizeit,thewholegenewasdividedintothreelargefragments(EP,PNandNH)whichwereassembledfromseveralchemicalsyntheticoligonucleotidesbyenzymaticmethod.TheassemblyofboththefragmentEPfromsixoligonucleotides(A-F)andthefragmentPNfromfouroligomers(G-J)wascatalyzedbyT4DNAligaseinusingthesinglestrandedDNAmethod[Chen,H.-B.etal,Nucl.AcidsRes.,18,871(1990)].AndfragmentNHwasformedfromthreeduplexesK,LandMbytheclassicaldoublestrandedDNAmethod.Finally,

简介：Withtheexpansionofthegeneexpressionprofiledatabase,inthecaseofaslittleaspossibletoloseinformationortoretainthemostcriticalinformation,geneextractionhasbecomeamaindirectionforthescholars.Thispaperexcludes1561irrelevantgenesthroughthedefinitionofweighteddistancefirstly,andthenremoves252redundantgenesbyPearson'scorrelationcoefficient.Finallybycomparingthetwomethods,stepwiseregressionafterclusteringandonlystepwiseanalysis,weobtainthebestcombinationof8genes.

简介：Cationic聚合物作为非病毒的基因向量一直在收到许多注意。水的活动阶段为尺寸排除与ShodexOHpakSB列在联合被优化层析(秒)分析包含二硫化物(乙烯imine)(PEI)poly，衍生物在基因交货使用了。在活动阶段的acetonitrile的增加被显示能压制在聚合物analytes和静止阶段之间的恐水病的相互作用。cationic聚合物的绝对分子的重量和分布从联机SEC-MALS(散布的多角度光)直接被决定/RI(折射索引察觉者)。结果证明在基因交货使用，小乐队拓宽的包含二硫化物的PEI衍生物的好秒分离被完成。

简介：为增加的目的在里面polycation基因搬运人的vivo稳定性，我们准备了一种pH敏感poly(乙烯乙二醇)-poly(-benzyl-L-glutamate-co-glutamic酸)(木钉针网阵列(65)，65表示glutamic酸的臼齿的比率在poly(-benzyl-L-glutamate-co-glutamic酸))。木钉针网阵列(65)显示出低cytotoxicity并且能高效地防护DNA/PEI(1:1)polyplexes的正电荷。transfection被提高由于部分收费在7.4的pH在HeLa房间线防护。有几乎没有transfection效率当第三的粒子的表面费用转到时在7.4的pH否定。然而，transfection效率在transfection的开始在6.0的pH由culturing恢复了很多。共焦的显微镜的观察和流动cytometry结果证明DNA/PEIpolyplexes应该高效地被释放并且在pH的endocytosized6.0，因为pH木钉针网阵列(65)的触发的deshielding行动。由于好biocompatibility和合适的pH触发了shielding/deshielding性质，木钉针网阵列(65)能是为在vivo使用的polycationic基因搬运人的一个潜在的防护系统。

简介：SmallRNAhasrecentlydrawnmoreandmoreattention.Inthispaper,weconcentrateontheinfluenceofnoisesongenenetworkregulatedbysmallRNAusingchemicalLangevinequation.Itshowsthatthenoisecancauseoscillationwhentheoscillatedoesnotoccurinthecorrespondingdeterministicsystem.Thecoherenceofthenoiseinducedoscillationreachesamaximumforanoptimalintensityofnoise,andthecoherenceresonanceappearsaccordingly.Thefindingsimplyprobablyomnipresentimportanceofnoiseinthefunctioningprocessoflivingorganism.

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简介：Inrecentyears,heavyionbeamshavebeenrecognizedasaneffectiveandefficientphysicalmutagenduetotheircapacitytoinducemutationswithhighfrequencyandbroadspectrum.Nowadays,itisnotsodifficulttoproducevariousmutantsinplants.However,tominetheresponsiblemutatedgeneshasbeenanimportantchallenge.Formutationisolation,map-basedcloningisoneofthemajortraditionalwaystoisolatethemutantgenesthatcontroltraitsofinterestinforwardgeneticsstudies.However,theprocessofmap-basedcloningisusuallycomplicatedandtime-consuming.