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399 个结果
  • 简介:ObjectiveThestudyistoidentifythecarrierrateofcommondeafnessmutationinChinesepregnantwomenviadetectingdeafnessgenemutationswithgenechip.MethodsThepregnantwomeninobstetricclinicwithouthearingimpairmentandhearingdisordersfamilyhistorywereselected.Theinformedconsentwassigned.PeripheralbloodwastakentoextractgenomicDNA.Applicationofgeneticdeafnessgenechipfordetecting9mutationalhotspotofthemostcommon4Chinesedeafnessgenes,namelyGJB2(35delG,176del16bp,235delC,299delAT),GJB3(C538T),SLC26A4(IVS72A>G,A2168G)andmitochondrialDNA12SrRNA(A1555G,C1494T).Furthergenetictestingwereprovidedtothespousesandnewbornsofthescreenedcarriers.ResultsPeripheralbloodof430pregnantwomenweredetected,detectionofdeafnessgenemutationcarriersin24cases(4.2%),including13casesoftheGJB2heterozygousmutation,3casesofSLC26A4heterozygousmutation,1casesofGJB3heterozygousmutation,and1caseofmitochondrial12SrRNAmutation.18spousesand17newbornstookfurthergenetictests,and6newbornsinheritedthemutationfromtheirmother.ConclusionThecommondeafnessgenesmutationhasahighcarrierrateinpregnantwomengroup,235delCandIVS7-2A>Gheterozygousmutationsarecommon.

  • 标签: 基因突变检测 基因芯片 遗传性 耳聋 孕妇 应用
  • 简介:Genestellcellswhattodo,forexample,whentorepairDNAmistakesorwhentodie,andcanbeturnedonorofflikealightswitch.Knowingwhichgenesareswitchedon,orexpressed,isimportantforthetreatmentandmonitoringofdisease.Now,forthefirsttime,Researchersinthelaboratoryof

  • 标签: 基因表达 可视化 MRI DNA 光开关 细胞
  • 简介:Wehavereviewedthegenetherapyingastrointestinaldiseases^[1].GastriccanceriscommoninChina^(2-20),anditsearlydiagnosisandtreatmentarestilldifficultuptonow^(13-36).Theex-pressionofanexogenousgeneintroducedbygenetherapyintopa-tientswithgliomascanbemonitorednon-invasivelybypositron-emissiontornography^[4].

  • 标签: 胃癌 胃肠疾病 肿瘤 治疗方法 氮化酶
  • 简介:Objective:TostudythechangesofthegeneexpressionpatternofspinalcordtissuesintheearlystageafterinjurybyDNAmicroarray(genechip).Methods:ThecontusionmodelofratspinalcordwasestablishedaccordingtoAllen'sfallingstrikemethodandthegeneexpressionpatternsofnormalandinjuredspinalcordtissueswerestudiedbygenechip.Results:Theexpressionof45geneswassignificantlychangedintheearlystageafterspinalcordinjury,inwhich22genesup-regulatedand23genesdown-regulated.Conclusions:Theexpressionofsomegeneschangessignificantlyintheearlystageafterspinalcordinjury,whichindicatesthecomplexityofsecondaryspinalcordinjury.

  • 标签: 脊髓损伤 早期阶段 基因表达类型 基因芯片检测
  • 简介:客观;在忍受肝癌症的老鼠调查鼠科的IL-12基因和HSV-TK基因治疗的synergistic反肿瘤效果。方法:老鼠肝癌症MM45T李(H-2d)房间是有包含IL-12基因或HSV-TK基因的retroviral向量的transfected插入修改基因的肝癌症房间,MM45TLi/IL-12和MM45TLi/TK,与IL-12和TK的稳定的表示被获得。Balb/c老鼠与2X105MM45T李房间皮下地被接种。当肿瘤到达了0.5鈥?.0厘米的一种尺寸时,MM45T.Li/TK房间的混合物照耀and60Co的MM45TLi/IL-12房间intratumoraly被注射。Ganciclovir(GCV)是注射ip(40mg.kg?1.d?1)为10天。Intratumoral注射照耀of60Co的MM45TLi/IL-12房间分开在一个星期内被重复两次。有远肿瘤的老鼠根据一样的协议被对待。怒气房间的CTL活动是测量by51Cr版本试金和由染色的immunohistochemical渗入淋巴细胞的肿瘤的显型。结果:在与MM45T对待的老鼠,Li/IL-12或MM45TLi/TK+GCV个别地在肿瘤生长,而是两个都不导致了中等减小能根除肿瘤完全,当时在加GCV与MM45TLi/IL-12和MM45TLi/TK细胞的混合物对待的60%老鼠,完全的肿瘤回归被观察,没有为二个月的肿瘤复发。远肿瘤的生长也在同样对待的老鼠显著地被禁止。大多数收到的老鼠加GCV联合了基因治疗有的丰富的CD4+,CD8+T淋巴细胞渗入。他们的CTL活动比在老鼠显著地高收到的单个基因治疗。有加GCV的IL-12基因和HSV-TK基因的结论联合治疗为老鼠肝癌症是有效的。

  • 标签: Liver cancer INTERLEUKIN-12 HSV-TK GENE therapy
  • 简介:MicroRNAs(miRNAs)是他们在植物和动物的目标基因的重要post-transcriptional管理者。miRNAs通常长是20-24核苷酸。尽管有他们的不平常地小的尺寸,miRNA基因家庭的进化历史似乎类似于编码theirprotein对应物。与在动物染色体的小却丰富的miRNA家庭相对照,植物有少数但是更大的miRNA基因家庭。植物miRNA基因家庭的成员经常是高度类似的,建议经由双人脚踏车基因复制和部分复制事件的最近的扩大。尽管许多miRNA基因越过植物种类被保存,一样的基因家庭在不同种类在尺寸和genomic组织显著地变化,它可以在目标基因规定引起剂量效果和空间、时间的差别。在这评论,我们在理解植物miRNA基因家庭的进化总结当前的进步。

  • 标签: 植物 miRNA基因家族 演化 转录后调节 串联复制 片段复制
  • 简介:ELEMENTALSTUDYOFGENETHERAPYWITHTHROMBOPOIETINLuChengrong陆承荣ZhaoJianzeng赵建增WangXiaoping王小平Liuli刘丽ResearchCenterofMolecularBiol...

  • 标签: 小平
  • 简介:Duetoconcernsregardingtheoverlappingfiguresinthisreviewthatareidenticaltothosecontainedinareviewarticlethatwehaveco-authoredandpublishedearlier,weretracttheabovepaperwepublishedinCellResearch.Weapologizeforanyconfusionthatmaybecausedbythismatter,althoughwestandbythescientificcontentscontainedintheCellResearchpaper.

  • 标签: 肿瘤 基因治疗 病理机制 临床
  • 简介:Asearlyas2000yearsago,ancientChinesemedicalrecordshaddescribedtherelationshipbetweendiseasesandappearanceindetail.Moreover,modernmedicinehasalsoconstantlystudiedtherelationshipbetweenfacialfeaturesandhealthinevolutionaryterms.Itiswellknownthatmanyhereditarydiseasesinvolvecertainabnormalfacialfeaturesandgenemutations.Thetumorisalsoconsideredasgeneticdisordertosomeextent,sowhatistherelationshipbetweencancergeneticsandcongenitaldevelopmentoffacialfeatures?Here,wereviewedsomecluestotheappearance-gene-tumorrelation,whichmightbecomethetargetsintheearlypreventionorgenetherapyofcancerinthefuture.Thissummaryprovidedusanewstrategyforthecancergeneticscreeningandanewresearchdirectionforgeneticdiagnosisofthepotentialdisease.

  • 标签: Chinese medical RECORDS appearance-gene-tumor GENETIC diagnosis
  • 简介:Directgenetransferintosomatictissueinvivoisadevelopingtechnologywithpotentialapplicationforcancergenetherapy.Retrovirusvector,whichwasaneffectivevehicle,stillhassomedisadvantagesingeneratinghightiterrecombinantvectorsandmanipulatingtomediateinvirogenetransfer.Inthispaper,recombinantvacciniavirusvectorencodinghuman

  • 标签: MELANOMA ENCODING disadvantages SOMATIC MURINE TITER
  • 简介:Thehomeobox(Hox)genesformanevolutionarilyconservedfamilyencodingtranscriptionfactorsthatplaymajorrolesinsegmentalidentityandorganspecificationacrossspecies.ThecanonicalgroupingofHoxgenespresentintheHOM-CclusterofDrosophilaorrelatedclustersinotherorganismsincludeseight"typical"genes,whicharelocalizedintheorderlabial(lab),proboscipedia(pb),Deformed(Dfd),Sexcombsreduced(Scr),Antennapedia(Antp),Ultrabithorax(Ubx),abdominalA(abdA),andAbdominalB(AbdB).ThemembersofHoxclusterareexpressedinadistinctanteriortoposteriororderintheembryo.AnalysisoftherelatednessofdifferentmembersoftheHoxgeneclustertoeachotherinfourevolutionarilydiverseinsecttaxarevealedthatthelocipb/DfdandAbdB,whicharefarthestapartinlinkage,hadahighdegreeofevolutionaryrelatedness,indicatingthatpb/DfdtypeanteriorgenesandAbdBareclosesttotheancestralanteriorandposteriorHoxgenes,respectively.ThegreaterrelatednessofotherposteriorgenesUbxandabdAtothemoreanteriorgenessuchasAntpandScrsuggestedthattheyarosebygeneduplicationsinthemoreanteriormembersratherthantheposteriorAbdB.

  • 标签: 昆虫 同源基因 基因复制 果蝇 胚胎发育
  • 简介:<正>Acutepromyelocyticleukaemia(APL)hasbeenattractingawideinterestfarbeyondhematologicalfieldinthelastdozenyearsduetothepresenceofspecificchromosometranlocationsandclinicalresponsibilitytoall-transretinoicacid(ATRA)bydifferentiationinductionaswellastoarsenictrioxide(ATO).Most(>95%)APLpatientscarryspecificchromosometranslocationt(15;17),whichleadstodiscoveryofPMLgeneonchromosome15.SuchatranslocationcausesthefusionofPMLtoretinoicacidreceptor-alpha

  • 标签: 白血病细胞 细胞凋亡 PML基因 三氧化二砷
  • 简介:Astructuralgene(750bp),whichcodesforatypeIribosomeinactivatingprotein,trichosanthin,hasbeendesignedaccordingtothecodonusageofhighlyexpressedgeneinE.coliandchemicallysynthesized.Inthesynthesizedgene,twenty-sevenuniquerestrictionsiteswereevenlydispersedwithanaveragedistancebetweentwoadjacentsiteslessthan50bptofacilitateasystematicinvestigationonstructure-functionalrelationshipofthisproteinbysite-directedmuta-genesis.Tosynthesizeit,thewholegenewasdividedintothreelargefragments(EP,PNandNH)whichwereassembledfromseveralchemicalsyntheticoligonucleotidesbyenzymaticmethod.TheassemblyofboththefragmentEPfromsixoligonucleotides(A-F)andthefragmentPNfromfouroligomers(G-J)wascatalyzedbyT4DNAligaseinusingthesinglestrandedDNAmethod[Chen,H.-B.etal,Nucl.AcidsRes.,18,871(1990)].AndfragmentNHwasformedfromthreeduplexesK,LandMbytheclassicaldoublestrandedDNAmethod.Finally,

  • 标签: RIBOSOME inactivating PROTEIN TRICHOSANTHIN GENE design
  • 简介:Withtheexpansionofthegeneexpressionprofiledatabase,inthecaseofaslittleaspossibletoloseinformationortoretainthemostcriticalinformation,geneextractionhasbecomeamaindirectionforthescholars.Thispaperexcludes1561irrelevantgenesthroughthedefinitionofweighteddistancefirstly,andthenremoves252redundantgenesbyPearson'scorrelationcoefficient.Finallybycomparingthetwomethods,stepwiseregressionafterclusteringandonlystepwiseanalysis,weobtainthebestcombinationof8genes.

  • 标签: STEPWISE regression CLUSTER ANALYSIS GENE EXTRACTION
  • 简介:Nanogproteinisexpressedintheinteriorcellsofcompactedmorulaeandmaintainedtillepiblastsbutdownregulatedbyimplantationstage.Itisalsoexpressedinembryonicstemcells,embryoniccarcinomacellsandembryonicgermcellsbutdisappearedindifferentiatedEScells.Inthisstudy,wehaveisolated,sequenced,andperformedthefirstcharacterizationoftheNanogpromoter.Thetranscriptionstartsitesweremappedbyprimerextensionanalysis.TwopromoterregionswerefoundupstreamthetranscriptionstartsitesandtheexpressionofmajorNanogpromoter/reportergeneconstructisabolishedindifferentiatedF9ECcellsascomparedtotheundifferentiatedcounterpart.Wealsoshowedthataputativeoctamermotif(ATGCAAAA)isnecessaryforthemajorpromoteractivity.GelshiftandsupershiftassaysshowedthatOct-1,Oct-4andOct-6proteinselectivelybindtotheoctamermotif.

  • 标签: 鼠科动物 NANOG基因 启动子 分离 F9 EC细胞
  • 简介:Withthedevelopmentofgenomesequencingformanyorganisms,moreandmorerawsequencesneedtobeannotated.Genepredictionbycomputationalmethodsforfindingthelocationofproteincodingregionsisoneoftheessentialissuesinbioinformatics.Twoclassesofmethodsaregenerallyadopted:similaritybasedsearchesandabinitioprediction.Here,wereviewthedevelopmentofgenepredictionmethods,summarizethemeasuresforevaluatingpredictorquality,highlightopenproblemsinthisarea,anddiscussfutureresearchdirections.

  • 标签: 基因 预测方法 相似性 马尔可夫模型 序列分析 人类基因组计划
  • 简介:obtainaninitialoverviewofgenediversityandexpressionpatterninporcinethymus,11,712ESTs(ExpressedSequenceTags)from100-day-oldporcinethymus(FTY)weresequencedand7,071cleanedESTswereusedforgeneexpressionanalysis.ClusteredbythePHRAPprogram,959contigsand3,074singletswereobtained.Blastsearchshowedthat806contigsand1,669singlets(totally5,442ESTs)hadhomologuesinGenBankand1,629ESTswerenovel.AccordingtotheGeneOntologyclassification,36.99%ESTswerecatalogedintothegeneexpressiongroup,indicatingthatalthoughthefunctionalgene(18.78%indefensegroup)ofthymusisexpressedinacertaindegree,the100-day-oldporcinethymusstillexistsinadevelopmentalstage.Comparativeanalysisshowedthatthegeneexpressionpatternofthe100-day-oldporcinethymusissimilartothatofthehumaninfantthymus.

  • 标签: 胎儿 胸腺 基因表达 基因差异
  • 简介:Objective:Tostudythevaccinepotencyofgene-modifiedtumorcells.Methods:TheEL-4lymphomawastransducedwithrecombinantretroviruscontainingthemurineGM-CSFgeneorB7-1gene.Theeffectofgenetransductiononantitumorimmunitywasinvestigated.Results:Flowcytometryanalysisshowedthatexpressionoftheirsurfacemarkerbetweenwild-typeEL-4cellsandgenetransducedtumorcellswasthesameexceptforCD80positiveinB7-1genetransducedcells.GM-CSFgeneorB7-1genetransducedEL-4cellsresultedinremarkablelossoftumorigenicityinsyngeneticmice.ThesystemicprotectiveimmunitywasinducedagainstthechallengewithEL-4/wtcells.TherapeuticvaccinewithEL-4/GM-CSForEL/7-1cellscouldretardthegrowthofestablishedearly-stageEL-4/wttumorsignificantly,butnotretardthegrowthoflate-stageEL-4/wttumor.IrradiatedGM-CSFgenetransducedEL-4cellsshowedstrongvaccineeffectagainstEL-4cellchallenge,butirradiatedB7-1genetransducedEL-4cellsshowedweakvaccineeffect.RemarkablecooperativeantitumoreffectagainstEL-4cellchallengewasobservedwhenbothirradiatedEL-4/GM-CSFandEL-4/B7-1wereinoculatedtogether.Conclusion:GM-CSFgeneorB7-1genetransducedcombinationofthetwokindsofvaccinemayhavepotentialapplicationvalueinhumancancertreatment.

  • 标签: GM-CSF B7-1 (CD80 ) 肿瘤疫苗 基因治疗