简介:为了更好地研究牙龈卟啉菌(Prophyromonasgingivalis,Pg)表面蛋白(Surface-associatedproteins,SAP)的生物活性,实验用SAP体外刺激小鼠颅骨和人外周血单个核细胞,分别用自动生化分析仪和放免方法检测培养液中Ca2+和IL-6的含量.同时,采用ELISA和MTT法分别测定SAP免疫小鼠的抗体效价和诱导的脾细胞转化程度.结果显示:一方面SAP是体外诱导骨吸收和前炎性因子合成的介质;另一方面SAP免疫的小鼠产生了抗SAP特异性抗体和脾细胞特异增生反应.这说明具有生物活性的SAP诱导了实验小鼠产生体液免疫应答和细胞免疫应答.
简介:以恒电位法在pH=9.0碱性水溶液中碳纤维簇电极上镀单层锌,在含有组氨酸和电解氧化锌镀层下原位合成了锌-组氨酸-羟基络合物修饰碳纤维电极,模拟了生物酶识别痕量的硫氢酸根离子,优化了电极制备的条件。建立了开路电位测定硫氢酸根离子的方法,在中性水溶液中,该电极以开路电位变化响应注入溶液中的硫离子浓度,并可用能斯特方程描述开路电位变化的规律。方法的最低检出限为1.0×10μmol/L,检测范围为1.0×10~1.0×10-18mol/L,相对标准偏差为3.5%。对实际样品中硫氢酸根离子检测结果为1.12×10-13mol/L,加标回收率为109.2%。研制的电化学传感器具有响应快速,灵敏度高,检出限低,检测范围宽,仪器简单方便等特点。
简介:Thesolvent-freemechanochemicalreactionandtheliquid-phasereactionofC60withethyl2-diazopropionatepreparedinsituorpreformedfromalanineethylesterhydrochlorideandsodiumnitritehavebeeninvestigated.Methanofullerene1andfulleroids2and3fromthesereactionswereobtained,meanwhilethepyrazolineintermediatewasnotobserved.Fulleroids2and3canbeconvertedtomethanofullerene1inrefluxingtoluene.
简介:Asasuccessiveworkofourpreviouspaper,^1theelectrontransfermatrixelement(Vrp)intheoxidationofthesimplifiedmodelmoleculeofα-aminocarbon-centeredradicalbyO2hasbeeninvestigatedwithabinitiocalculationatthelevelofUHF/6-31G**.Basedontheoptimizedgeometriesofthereactgantandtheion-paircomplexobtainedpreviously,thereactionheatandtheiunerreorganizationenergyhavebeenobtainedbyconstructingthepotentialenergycurvesofreactantandproductstatesconsideringthesolventeffectwiththeconductor-likescreeningmodel(COSMO).ThesolventreorganizationenergyhasbeenestimatedusingLippert-Matagarelationship.ThecalculatedresultsshowthatthevalueofVrpisseveraltimeslargerthanthatofRT,whichmeansthatthemodelreactionisanadiabaticone.Theoreticalinvestigationindicatesthatthesolventeffectonthedirectelectrontransfer(ET)processofoxidationofα-aminocarbon-centeredradicalbyoxygenisremarkable.
简介:Aseriesofchiralandachiralbis(2-oxazolinylphenolato)nickel(Ⅱ)complexes2weresynthesizedbyreactionsofvarious2-(4,5-dihydro-2-oxazolyl)phenolderivativeswithnickelchloridehexahydrate.Themolecularstructureofcomplex2awasdeterminedbyX-raysinglecrystaldiffraction.Thecrystalofcomplex2awasmonoclinic,spacegroupP21withcelldimensionofa=1.1121(4)nm,b=0.9472(3)nm,c=1.4113(5)nm,a=γ=90°,β=91.450(6)°,V=1.4861(8)nm^3,Z=2,andμ=0.693mm^-1.Inthesolidstate,thenickel(Ⅱ)ionwasinasquare-planarcoordinationgeometry.Thecatalyticactivitiesofcomplexes2inBaeyer-Villigerreactionwithmolecularoxygenwereinvestigated.
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简介:Fe2O3solwiththeparticlediameterof3-5nmwasflocculatedbytheadditionofSDS,andtheflocculateformedwasredispersedbythefurtheradditionofthatsurfactant.ThusthesurfactantbilayerwasformedonthesurfaceofFe2O3.Theemulsionpolymerizationofstyrene(St)adsolubilizedonthesurfactantadsorbedbilayerwascarriedoutbyinitiatorpotassiumpersulfate(KPS).TheUV-Visandsurfacephotovoltagespectra(SPS)indicatethattheFe2O3particleswereencapsulatedinpolystyrene(PSt)successfully.
简介:Thispaperreportstwolanthanidecomplexesofformula(C9H7)Ln(C8H8)·(THF)2whereLnisProrNd,C9H7isindenyl,andC8H8iscyclooctatetraene(COT).ThecomplexeswerepreparedbythereactionofLnCl3withK(C9H7)andK2(C8H8)inTHF.(C9H7)Pr(C8H8)·(THF)2crystallizesinTHFat-15℃inthemonoclinicspacegroupP21:withunitcelldimensionsa=8.446(0),b=10.083(2),c=13.407(3),β=105.48(1)°,V=1100.43(35)3,Dc=1.52g/cm3andZ=2.ThefinalRvalueis0.033,Rwvalueis0.030,respectively.In(C9H7)Pr(C8H8)·(THF)2afive-memberedringcentroidofC9H7,theC8H8ringcentroidandthetwooxygenatomsfromthetwoTHFmoleculesformadistortedtetrahedralgeometryaroundthemetal.
简介:8,8′-Dibromo-6,6′,7,7′-tetramethoxy-5,5′-diisopropyl-3,3′-oxydimethylenedinaphtho[2,1-b:2′,1′-d]furan,C32H30Br2O;M.W.=670;triclinic;spacegroupP1;a=8.896(2),b=10.216(1),c=10.423(1)A,α=108.48(1),β=119.36(1),γ=99.26(1);V=723.5A3;Z=1,Dcal.=1.479g.cm-3.MoKα=28.11cm-1,F(000)=326,T=293K.Thestructurewassolvedbydirectmethodandfull-matrixleast-squaresrefinementconvergedatR=0.034for2377observedreflections.Theoxygenatominseven-memberedringexistsinadisorderedarrangement.Bothbisnaphthaleneandoxygen-containingfive-memberedringhaveplanarconformationswithdihedralangle6°,showingmarkedaromaticfeature.
简介:Thetetranuclearalkyltin(Ⅳ)compounds{[R2Sn(C9H8N3O3)O]SnR3}2[R=n-Bu(1),4-CNC6H4CH2(2),C6H5CH2(3),4-ClC6H4CH2(4)]werepreparedbythereactionofSchiffbaseligandpyruvicacidisonicotinylhydrazonewith(R3Sn)2Ointhecorrespondingmolarratioof1:1.Allcompoundshavebeencharacterizedbyelementalanalysis,IRand^1HNMRspectra.Thecrystalstructureofcompound1wasdeterminedbyX-raysinglecrystaldiffractionalanalysis.Thiscompoundexhibitsadimericstructurecontainingdistannoxaneunitswithtwotypesofthetinatoms.Forthefirsttinatom,itappearstobeseven-coordinatedwithadistortedpentagonalbipyramidgeometry,andtheotherisfive-coordinatedwithadistortedtrigonalbipyramidalgeometry.Themoleculesarepackedintheunitcellintwo-dimensionalnetworkstructurethroughaninteractionbetweentheNatomsofthepyridineandthetinatomsofanadjacentmolecule.
简介:采用PCR法获得拟南芥叶绿体核糖体psrp-3基因序列并进行生物信息学分析.根据psrp-3基因序列保守区设计2对引物,通过RT-PCR获得预期大小的基因序列,琼脂糖凝胶电泳鉴定目的片段.利用生物信息学软件对拟南芥psrp-3基因序列进行同源比对、氨基酸组成、功能域、二级结构、疏水性、蛋白质功能及系统进化分析和预测.结果表明,RT-PCR获得了一段753bp的序列,psrp-3蛋白是等电点为9.27的亲水性稳定蛋白,包含一个结构域和一个区域,α螺旋和不规则卷曲是其蛋白质二级结构的主要结构元件,β折叠和伸展链散布其中,和菠菜、葡萄、蓖麻、大豆等的psrp-3蛋白有较高的同源性.为进一步了解psrp-3基因的功能和作用机制打下基础.
简介:ThespinelferritesMnFe2O4nanowiresweresynthesizedbyhydrothermalroute,porousMnFe2O4andnanoparticlesmorphologiesweresynthesizedbysol-gelmethodwitheggwhite.Thestructures,morphologies,magneticpropertiesandadsorptionpropertiesoftheseobtainedferriteswithdifferentmorphologieswerestudiedcontrastively.Resultsshowthattheobtainedsamplesexhibitferromagneticproperties.Thisrealizesconvenientmagneticseparationfromsolutionwhentheyareusedinthetreatmentoforganicdyeswastewater.However,thecontrastivestudiesshowthatthesaturationmagnetizations(Ms)ofMnFe2O4withdifferentmorphologiesaredifferentandtheMsfollowstheorder:Ms(porous)