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简介：Onceuponatime,amothermouseandheryoungchildrenwentforawalkinthegarden.Theywerelookingaroundforsomethingtoeatwhentheysuddenlyheardaloudnoise.“Hiss,Hiss,Meow!”Itwasthevoiceof,thecat.

简介：一、故事内容Thereisafamilyofmiceinmyhouse.Theyarefathermouse,mothermouseandbabymouse.Babymouselikesdancing.Heisverycute.FathermouselikeswatchingTV.HelikesthesportsonTVbest.Thesethreemiceareclever.

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简介：Aprimaryhumanrenalcellcarcinomawasdevelopedasaxenograft(NT-25)andmaintainedbyserialtransplantationinnudemice.TheeffectofUFTonthisneogrowthwastestedandevaluatedaswellitsdistributionintheanimaltissues.TheconcentrationofUFTwashigherintumortissuesthanthatinothertissuesandintheanimalexperimentationUFTwasfoundtobeeffectiveonhumanrenalcellcarcinoma.

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简介：Apoptosisorprogrammedcelldeath(PCD)isanevolutionarilyconservedcellularprocessthatisessentialfornormaldevelopmentandhomeostasisofmulticellularorganisms.Defectsintheapoptosissignalingresultinmanydiseasesincludingautoimmunediseasesandcancer.TheapoptosissignalingpathwaywasfirstdescribedgeneticallyinthenematodeCaenorhabditiseleganswhichservesasaframeworkforthemorecomplexapoptoticpathwaysthatexistinmammals.Inthisreview,wewilldiscusstheapoptoticpathwaysthatareemerginginmammalsaselucidatedbystudiesofgene-targetedmutantmice.

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简介：AbstractBackground:Acinetobacter baumannii (A. baumannii) has become one of the most important opportunistic pathogens inducing nosocomial pneumonia and increasing mortality in critically ill patients recently. The interaction between A. baumannii infection and immune response can influence the prognosis of A. baumannii related pneumonia. The target of the present study was to investigate the role of immunodeficiency in A. baumannii induced pneumonia.Methods:Male BALB/c mice were randomly divided into the normal immunity control (NIC) group, normal immunity infection (NIA) group, immune compromised control (CIC) group, and immune compromised infection (CIA) group (n = 15 for each group). Intraperitoneal injection of cyclophosphamide and intranasal instillation of A. baumannii solution were used to induce compromised immunity and murine pneumonia, respectively. The mice were sacrificed at 6 and 24 h later and the specimens were collected for further tests. Seven-day mortality of mice was also assessed.Results:After A. baumannii stimulation, the recruitment of neutrophils in mice with normal immunity increased sharply (P = 0.030 at 6 h), while there was no significant raise of neutrophil counts in mice with compromised immune condition (P = 0.092 at 6 h, P = 0.772 at 24 h). The Th cell polarization presented with pulmonary interleukin (IL)-4 and interferon (IFN)-γ level in response to the A. baumannii in CIA group were significantly depressed in comparison with in NIA group (IFN-γ: P = 0.003 at 6 h; P = 0.001 at 24 h; IL-4: P < 0.001 at 6 h; P < 0.001 at 24 h). The pulmonary conventional dendritic cell accumulation was even found to be inhibited after A. baumannii infection in immunocompromised mice (P= 0.033). Correspondingly, A. baumannii associated pneumonia in mice with compromised immunity caused more early stage death, more severe histopathological impairment in lung.Conclusion:A. baumannii could frustrate the immune response in immunocompromised conditions, and this reduced immune response is related to more severe lung injury and worse outcome in A. baumannii induced pneumonia.

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简介：AbstractSevere fever with thrombocytopenia syndrome (SFTS), caused by a novel identified bunyavirus SFTS virus (SFTSV), was an emerging viral infectious disease that was firstly reported in China. There are no licensed vaccines and therapeutics against SFTSV currently. B-Propiolactone (BPL) inactivated whole virions of SFTSV strain AH12 were prepared as experimental vaccine in different antigen dose with or without Al(OH)3 adjuvant. The experimental SFTS vaccine was a satisfying immunogen, which could efficiently trigger the development of high levels of SFTSV NP-specific IgG antibodies and neutralizing antibodies against SFTSV Strain HB29 in BALB/c and C57/BL6 mice, and could induce SFTS virus-specific cellular immune responses to a certain extent. A single dose of vaccine was immunogenically insufficient in BALB/c mice; the second and third dose resulted in significant boost in antibody response. The use of Al(OH)3 adjuvant resulted in higher antibody titers. The mediate-dose of vaccine could induce as high and equivalent level of antibody titer as that of high-dose. The experimental SFTS vaccine in mediate-and high antigen dose with adjuvant resulted in solid protection of C57/BL6 mice against wild-type SFTSV challenge with markedly accelerated virus clearance from blood and spleen compared with controls. The experimental SFTS vaccine prepared in this study could efficiently elicit virus specific humoral and cellular immune responses in both BALB/c and C57/BL6 mice, and could protect C57/BL6 mice against SFTS virus challenge. These results supplied evidence that inactivated vaccine was a promising vaccine candidate for the prevention of SFTSV infection.

简介：Objective:Tounderstandthecrucialroleoftheklothogeneinhearingdevelopmentinmousemodels.Methods:PCRwasusedtoidentifyCBAmicewithdifferentgenotypes,i.e.WT,heterozygous(klotho+/-)orhomozygous(klotho-/-).Micephenotypeandweightwererecordedpostnatal25days(P-25)andauditorybrainstemresponses(ABR)wereusedtodetermineauditoryfunctionatP-60.Results:klotho-/-micetendedtohavesmallersize,lighterweightandhigherABRthresholdsatP-60,showingearlyonsetage-relatedhearingloss(ARHL).Conclusion:Heterozygousandhomozygousklothodeficientmiceexhibitdifferentdegreesofhearinglossatyoungage,withhomozygousmice(klotho-/-)showingmoreseverehearingloss.OurresultsindicatethatpersistedexpressionofklothoproteinintheinnerearmaypotentiallydelaytheonsetofARHLandplayanimportantroleintheprotectionofauditoryfunction.

简介：DistributionandaccumulationofNd,anditseffectonsecretionofprogesteroneinmicewerestudiedusingradioisotopetracer(147Nd)technique.Followingsingleintraperitonealadministrationofneodymiumtracedwith147Ndatadoseof200mg*kg-1,unevendistributionoftheradioactiveNdoccurredinvarioustissuesandorgans.Muchamountof147Ndaccumulatesinthebone,andtheresidueincreaseswiththelapseoftime.Someamountofradioactivitywasalsodetectedineyes,bloodandbrain,buttheaccumulationdecreasedwiththetimeduetoexcretionandre-distributioninmice.Incomparisonwithcontrols,concentrationofprogesteroneisfoundtobesignificantlylowerintheserumofadministeredmice,indicatingasignificantlyinhibitoryeffectofNdonsecretionofprogesterone.

简介：Objective:Todescribeaprotocolforrecordingelectricalpotentialsfromthescalamedia,saccule,andutricleinmice.Method:CBA/Jmicewereusedandpotentialswererecordedwithglasselectrodesinsertedthroughthebasilarmembraneusingapatchclampsystem.Results:Restingpotentialsweresuccessfullyrecordedfromthescalamedia,sacculeandutricleusingdescribedprotocols.Conclusions:Withthemethoddescribed,onecanmeasurerestingpotentialsfromthescalamedia,sacculeandutricle,aswellascochlearmicrophonics(CM)andevenauditorynervecompoundactionpotentials(CAP),inasinglemouse.

简介：肺结核仍然是全球传染的疾病。识别M的治疗学的潜力。在对待肺结核的vaccae，M。vaccae被注入Mycobacterium肺结核(M。肺结核)感染的老鼠。M的最佳的剂量。对待的老鼠显示出的vaccae(22.5g/mouse)降低病理学的变化索引，怒气重量索引，肺重量索引和重要M。肺结核未经治疗的组比那些数。有M的治疗。vaccae提高了CD3+和CD4+T房间，IFN-+CD4+T房间，包括NK房间的天生的有免疫力的房间，NK1.1+T房间和T房间的百分比，并且减少了IL-4+CD4+T房间的百分比。因此，M。vaccae能保护老鼠免受M的伤害。肺结核感染和天生的改进老鼠和适应装壁板圩调停免疫，建议那M。vaccae是在肺的肺结核的一个潜在的immunotherapeutic代理人。

简介：Anoveltuberculosis(TB)genevaccinecontainingmousegranulocytemacrophage-colonystimulatingfactor(mGM-CSF)andaTBantigen(Ag85A)wasdevelopedinthisstudy.ThegenesencodingAg85AandmGM-CSFwereamplifiedbyPCRrespectivelyfromtheAg85A-containingpBSby5andpC-mGM-CSF.ThegeneswerethenclonedintotwodifferentpolylinkersitesofplasmidpIRES,forminganovelTBgenevaccineconstructpI85AGM.FollowingtransfectionofpI85AGMplasmidinto7721celllinebyLipofectamineTM,theexpressionofAg85AandGM-CSFproteinswasidentifiedbyWesternblottingorRT-PCR.ThenBalb/cmicewereinoculatedwiththerecombinantpI85AGM,pI85A,pIGMorplasmidalone,respectively.TheactivitiesofCTL,NKcellsandtheAg85A-stimulatedproliferationofspleencellsweremeasuredbyMTTmethod.TheserumantibodyagainstAg85AwasdetectedbyELISA.TheresultsshowedthattheAg85AandGM-CSFproteinscouldbeexpressedin7721celllineandtheactivityofCTLsandtheproliferationofspleencellsweresignificantlyincreasedinthepI85AGM-immunizedmice,indicatingthatthepI85AGM-immunizedmicecouldgeneratespecificimmuneresponsestoAg85A.Thisstudymightprovidepossibilityfordevelopingnovelanti-TBgenevaccine.

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