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简介：Physiognomyisamethodofobservingpeople.Accordingtoone'sappearance,ancientChineseTaoistpriestcanpredicthispersonality,physicalcondition,lifeexperience,andfatebyphysiognomy.AncientChinesephysiognomycanbetracedbacktothe7thcenturyBC.Accordingtothehistoricalrecords,aministerwasabletojudgeprince'sabilitiesanddestiniesbyhisfacialfeaturesintheSpringandAutumnperiod.

简介：Chinesenongovernmentalorganizations(NGOs)haveplayedasignificantroleinthebattleagainstAIDSinthePeople'sRepublicofChina.Thisarticleprovidesabriefoverviewofthestructureoftheseorganizations,aswellasananalysisoftheirprincipleaccomplishments.OfgreatsignificanceinthisanalysisisthefactthatChineseNGOshaveeffectivelydealtwithmanysensitivehealtheducationareasthatgovernmentauthoritieshavefeltreluctanttohandledirectly.Assuch,theyhaveprovidedanindispensablecomponentintheHIV/AIDSpreventionandcontrolcalculusonthemainland.

简介：Wolbachia是感染大量节肢动物和丝虫的线虫的细胞内部的共生者。蚜虫从事与他们的endosymbionts的多样、复杂的关系。Wolbachia的四超群(A，B，M和N)以前在蚜虫和超群M被检测，N仅仅在蚜虫被发现。在这研究，我们在中国在蚜虫的自然人口检测了并且描述Wolbachia感染。三超群(A，B和M)在检验蚜虫种类被发现。超群M是优势的，而超群A和B仅仅在某些种类被检测。超群N没在这研究被发现。也就是，有在蚜虫的Wolbachia的四个感染模式有超群M的感染独自一个，有超群A和M，有超群B和M的合作感染，和有超群A，B和M的合作感染的合作感染。仅仅有超群M的感染的模式是通用的并且在所有评估亚科被发现。仅仅二个亚科，Aphidinae和Lachninae，表明了介绍所有四个感染模式。三个模式在Calaphidinae被观察(M，A&M，B&M)并且Eriosomatinae(M，B&M，A&B&M)。二个模式在Anoeciinae被观察(M，A&M)并且Greenideinae(M，B&M)，并且一仅仅模式(M)在Aphidoidea的留下的家庭或亚科被观察。这些结果显示在中国蚜虫的Wolbachia感染是普遍的。种系发生的分析建议超群M在中国在蚜虫的所有主人种类之中很快并且最近散布了的那Wolbachia。为这传播和它的机制的原因在他们的蚜虫主人上与Wolbachia的可能的效果一起被讨论。

简介：Nineshorttandemrepeat(STR)markers(D3S1358,VWA,FGA,THO1,TPOX,CSFIPO,D5S818,D13S317,andD7S820)andasex-identificationmarker(Amel-ogeninlocus)wereamplifiedwithmultiplexPCRandweregenotypedwithafour-colorfluorescencemethodinsamplesfrom174unrelatedHanindividualsinNorthChina.Theallelefrequencies,genotypefrequencies,heterozygosity,prob-abilityofdiscriminationpowers,probabilityofpaternityexclusionandHardy-Weinbergequilibriumexpectationsweredetermined.TheresultsdemonstratedthatthegenotypesatalltheseSTRlociinHanpopulationconformtoHardy-Weinbergequilibriumexpectations.Thecombineddiscriminationpower(DP)was1.05×10-10withinnineSTRlocianalyzedandtheprobabilityofpaternityexclusion(EPP)was0.9998.TheresultsindicatethatthesenineSTRlociandtheAmelo-geninlocusareusefulmarkersforhumanidentification,paternityandmaternitytestingandsexdeterminationinforensicsciences.

简介：Allelefrequenciesfor15shorttandemrepeat(STR)loci(D8S1179,D21S11,D7S820,CSF1PO,D3S1358,TH01,D13S317,D16S539,D2S1338,D19S433,vWA,TPOX,D18S51,D5S818,andFGA)wereobtainedfrom7,636unrelatedindivid-ualsofChineseHanpopulationlivinginQinghaiandChongqing,China.Totally206alleleswereobserved,withthecorrespondingallelefrequenciesrangingfrom0.0001–0.4982.Chi-squaretestshowedthatalloftheSTRlociagreedwiththeHardy-Weinbergequilibrium.Wealsocomparedourdatawithpreviouslypub-lishedpopulationdataofotherethnicsorareas.TheresultsarevaluableforhumanidentificationandpaternitytestinginChineseHanpopulation.

简介：TheexperimentwasperformedtoevaluatetheprogeniesofplantlinestransgenicforauxinsynthesisgenesderivedfromRiT-DNA.Fourlinesofthetransgenicplantswereself-crossedandtheforeignauxingenesinplantsofT5generationwereconfirmedbySouthernhybridization.Twolines,D1232andD1653,showedearlierfoldingofexpandingleavesthanuntransformedlineandthereforehadearlyinitiationofleafyhead.LeafcuttingsderivedfromplantoftransgeniclineD1653producedmoreadventitiousrootsthanthecontrolwhereasthecuttingsfromfoldingleaveshadmuchmorerootsthanrosetteleavesatfoldingstage,andthecuttingsfromheadleaveshadmorerootsthanrosetteleavesatheadingstage.Itisdemonstratedthatearlyfoldingoftransgenicleafmaybecausedbytherelativelyhigherconcentrationofauxin.Theseplantlineswithauxintransgenescanbeusedforthestudyofhormonalregulationindifferentiationanddevelopmentofplantorgensandforthebreedingofnewvarietywithrapidgrowthtrait.

简介：AsapartofabasicresearchprojectonXeno-transplantion,wehavebeenengagedinthederivationofembryonicstemcelllinesfromChineseminiswine.Here,wereportedforthefirsttimetheestablishmentoftwoporcineEGcelllines(BPEG1andBPEG2)fromprimordialgermcellsofgenitalridgesofa28anda27dembryosrespectively.Theirpluripotentnaturehasbeenidentifiedbycolonymorphology,markercharacterizationaswellasbyinvitroandinvivodifferentiation.TheseporcineEGcellsarepotentiallyusefulforfurtherbasicstudies.

简介：塔兰图拉毒蛛毒液为学习毒素选择，结构活动关系和肽毒素的分子的进化提供一个模型系统。以前的研究鉴别了中国鸟的毒液地的很多肽毒素搜寻Haplopelmahainanum，通常认为是一只高度有毒的蜘蛛。然而，可得到的RNA-seqtranscriptomic的缺乏和genomic数据是到在分子的水平理解它的毒液的一个障碍。在这研究，我们调查了H的毒液腺transcriptome。由RNA-seq的hainanum，当一个可得到的genomic序列不在时。我们鉴别在57181denovo之中的201潜在的毒素装配了抄本，包括knottins，Kunitz类型毒素，酶和另外的蛋白质。我们系统地识别了大多数knottins和Kunitz类型毒素，其中一些强烈出现了，在毒液腺偏导表示包括huwentoxin-1的成员，huwentoxin-2和magi-1家庭。我们也发现了几新奇潜在的毒素。这些数据在H的毒液毒素表明高分子、结构的差异。hainanum。这研究为探索蜘蛛毒液的复杂部件提供有用策略。

简介：中国白蜡规模昆虫，Ericeruspela，能在-30°C下面在温度在极其低的温度，和某overwintering个人展览supercooling幸存。调查E的深supercooling能力。pela，transcriptomic和proteomic分析被执行描出为overwintering女性的深supercooling能力负责的主要基因和蛋白质家庭。基因本体论(去)基因在表明小径和小径的激活mitogen的蛋白质kinase，钙，和PI3K-Akt包含了，这与可溶的糖，糖白酒和免费氨基酸的生合成联系的分析显示了的基因和染色体(KEGG)的分类和京都百科全书是主导的。为低温度的应力负责的蛋白质例如冷环境适应蛋白质，甘油生合成相关的酶和热吃惊蛋白质(HSP)被识别。然而，防冻剂蛋白质(法新社)都没通过顺序类似搜索方法被识别。一条随机的森林途径在proteome识别了388个通常认为的法新社。法新社基因ep-afp在Escherichiacoli被表示，并且表示蛋白质展出了0.97°C的一项热磁滞现象活动，建议它处于E的深supercooling能力的潜在的角色。pela。

简介：Humanleukocyteantigen(HLA)systemisthemostpolymorphicregionknowninthehumangenome.Inthepresentstudy,weanalyzedforthefirsttimetheHLA-Agenepolymorphismsdefinedbythehigh-resolutiontypingmethods-sequence-basedtyping(SBT)in161NorthernChineseHanpeople.Atotalof74differentHLA-Agenetypesand36allelesweredetected.ThemostfrequentalleleswereA110101(GF=0.2360)，A24020101（GF=0.1646），andA020101(GF=0.1553)；followedbyA3303(GF=0.1180)，A3001（GF=0.0590)，andA310102(GF=0.0404).Thefrequenciesoffollowingalleles,A0203,A0205,A0206,A0207,A030101,A2423,A2601,A3201,andA3301,areallhigherthan0.0093.ThehomozygousallelesincludeA020101,A110101,A24020101andA310102.Heterozygosity(H),polymorphisminformationcontent(PIC),discriminationpower(DP)andprobabilityofpaternityexclusion(PPE)ofHLA-Ainthesampleswerecalculatedandtheirvalueswere0.8705，0.8491，0.6014，and0.9475，respectively.Hanpopulation，especiallytheallelesubtypescharacter,willbeofgreatinterestforclinicaltransplantation,disease-associatedstudyandforensicidentification.Implementationofhigh-resolutiontypingmethodsallowsasignificantlywiderspectrumofHLAvariationincludingrarealleles.Thisrpectrumwillfurtherbeextensivelyutilizedinmanyfields.