简介：Nineshorttandemrepeat(STR)markers(D3S1358,VWA,FGA,THO1,TPOX,CSFIPO,D5S818,D13S317,andD7S820)andasex-identificationmarker(Amel-ogeninlocus)wereamplifiedwithmultiplexPCRandweregenotypedwithafour-colorfluorescencemethodinsamplesfrom174unrelatedHanindividualsinNorthChina.Theallelefrequencies,genotypefrequencies,heterozygosity,prob-abilityofdiscriminationpowers,probabilityofpaternityexclusionandHardy-Weinbergequilibriumexpectationsweredetermined.TheresultsdemonstratedthatthegenotypesatalltheseSTRlociinHanpopulationconformtoHardy-Weinbergequilibriumexpectations.Thecombineddiscriminationpower(DP)was1.05×10-10withinnineSTRlocianalyzedandtheprobabilityofpaternityexclusion(EPP)was0.9998.TheresultsindicatethatthesenineSTRlociandtheAmelo-geninlocusareusefulmarkersforhumanidentification,paternityandmaternitytestingandsexdeterminationinforensicsciences.

简介：Allelefrequenciesfor15shorttandemrepeat(STR)loci(D8S1179,D21S11,D7S820,CSF1PO,D3S1358,TH01,D13S317,D16S539,D2S1338,D19S433,vWA,TPOX,D18S51,D5S818,andFGA)wereobtainedfrom7,636unrelatedindivid-ualsofChineseHanpopulationlivinginQinghaiandChongqing,China.Totally206alleleswereobserved,withthecorrespondingallelefrequenciesrangingfrom0.0001–0.4982.Chi-squaretestshowedthatalloftheSTRlociagreedwiththeHardy-Weinbergequilibrium.Wealsocomparedourdatawithpreviouslypub-lishedpopulationdataofotherethnicsorareas.TheresultsarevaluableforhumanidentificationandpaternitytestinginChineseHanpopulation.

简介：Humanleukocyteantigen(HLA)systemisthemostpolymorphicregionknowninthehumangenome.Inthepresentstudy,weanalyzedforthefirsttimetheHLA-Agenepolymorphismsdefinedbythehigh-resolutiontypingmethods-sequence-basedtyping(SBT)in161NorthernChineseHanpeople.Atotalof74differentHLA-Agenetypesand36allelesweredetected.ThemostfrequentalleleswereA110101(GF=0.2360)，A24020101（GF=0.1646），andA020101(GF=0.1553)；followedbyA3303(GF=0.1180)，A3001（GF=0.0590)，andA310102(GF=0.0404).Thefrequenciesoffollowingalleles,A0203,A0205,A0206,A0207,A030101,A2423,A2601,A3201,andA3301,areallhigherthan0.0093.ThehomozygousallelesincludeA020101,A110101,A24020101andA310102.Heterozygosity(H),polymorphisminformationcontent(PIC),discriminationpower(DP)andprobabilityofpaternityexclusion(PPE)ofHLA-Ainthesampleswerecalculatedandtheirvalueswere0.8705，0.8491，0.6014，and0.9475，respectively.Hanpopulation，especiallytheallelesubtypescharacter,willbeofgreatinterestforclinicaltransplantation,disease-associatedstudyandforensicidentification.Implementationofhigh-resolutiontypingmethodsallowsasignificantlywiderspectrumofHLAvariationincludingrarealleles.Thisrpectrumwillfurtherbeextensivelyutilizedinmanyfields.