The culture and establishment of embryonic germ (EG) cell lines from Chinese mini swine

(整期优先)网络出版时间:2003-03-13
/ 1
AsapartofabasicresearchprojectonXeno-transplantion,wehavebeenengagedinthederivationofembryonicstemcelllinesfromChineseminiswine.Here,wereportedforthefirsttimetheestablishmentoftwoporcineEGcelllines(BPEG1andBPEG2)fromprimordialgermcellsofgenitalridgesofa28anda27dembryosrespectively.Theirpluripotentnaturehasbeenidentifiedbycolonymorphology,markercharacterizationaswellasbyinvitroandinvivodifferentiation.TheseporcineEGcellsarepotentiallyusefulforfurtherbasicstudies.