简介：InordertoobserveseveralantibodiestoliverantigensinChinesepatientswithdifferentfiverdiseasesandtodiscussthecharacteristicsoftheautoantibodiesinautoimmuneliverdiseases,from1412patients,detectedbyindirectimmumofluoreseence(IIF)initially,230patientswithabnormalALTwerechosenanddividedinto5groups:①autoimmunediseasesgroup,42cases:18withautoimmtmehepatitis(AIH),21withprimarybiliarycirrhosis(PBC),3withprimarysclerosingcholangitis(PSC).②HAVgroup,23cases;③HBVgroup,70cases;④HCVgroup,35casesand⑤NonA-Egroup,60cases.First,ANA,AMA,SMA,liver-kidneymicrosomalantibody(LKM)andsoonweretestedby1/F.Then,LKM-1,fivercytosofic-1(LC-1),solubleliverantigen/fiverpancreas(SLA/LP)andsubtypeofAMA(M2)aswellasANAprofilesuchasSS-A,SS-BanddsDNAweretestedbyWesternblotandimmtmoblotstripsassay,respectively.Theresultswerethatamong1412cases,thosediagnosedasAIH,PBCandPSCaccotmtedfor12.7‰,14.9‰and2.1‰,respectively,ofthesamplesbeingtested.2/230withLKM-1and2/230withSLA/LPwereseeninindividualsinfectedwithAIHandHCV,respectively.AllpatientswithPBCshowedAMAandM2antibodies.NospecificANApatternwasseeninAIHby1/Fbutanti-actinwasonlyfoundinpatientswithAIH.InNonA-Egroup,fourcaseswerepositiveofAMAandM2;threehadhightiterofSMAandother4hadSS-A,SS-BordsDNAantibodies,etc.Itwasconcludedthatthedetectionofanti-fiverantigens,ANAprofileandAMAsubtypeswerehelpfulforthediagnosisofautoimmunefiverdiseasesandoverlapsyndromes.InpatientswithNonA-Ehepatitis,thediagnosisofPBCorAIHshouldbetakenintoconsideration.

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简介：Inthepresentstudy,thedrug-resistancegenesencodingβ-lactamases,aminoglycosidemodifyingenzymes,DNAtopoisomerasesandintegronaswellastheirmolecularepidemiologywereinvestigatedbymeansofanalyzingthedrug-resistanceandmolecularepidemiologyofAcinebacterbaumanniiisolatedfromtheclinicalsamplesintwohospitalsinQiangzhouandHuzhoucityofJiangsuandZhejiangprovincefromJuly2000toMarch2005.Theminimalinhibitoryconcentrations(MICs)ofthese307isolatesweredetectedbyautomaticmicrobiologicalsystem,and35strainsagainst5-fluoro-quinoloneswereperformedbyagardilutionassay.Meanwhile,theresistantgenesin80isolateswereamplifiedbyPCRwithidentificationbyDNAsequencer.Itwasfoundthatmostofthe307isolatesofA.baumanniiwereresistanttomultipleantibioticstested,inwhichtheresistanceratesoftheisolatesagainstpiperacillin,piperacillin/tazobactam,amoxacillin/clavulanicacid,cefotaxime,ceftazidime,cefepime,gentamicin,amikacin,ciprofloxacin,chloramphenicolandsulfamethoxazole/trimethoprimwereallabove35%,butthoseofimipenemandmeropenemwerequitelow,rangedonly2.6%and3.3%.Inaddition,itwasalsodemonstratedthatthepositiveratesofTEMandSHVβ-lactamasegenesaccountedfor93.8%and22.5%respectively,andthoseoftheaminoglycoside-modifyingenzymegenesincludingaacC1,aacC2,aacC3,aacC4,aacC4A,aphA6,ant(2')-Iandant(3')Iwere58.8%,8.8%,7.5%,28.8%,45.0%,2.5%,28.8%and65.0%respectively.Themutationsinthequinolone-resistantdeterminingregion(QRDR)ofgyrAandparCgenesindicatedthatsubstitutioninSer-83residueofGyrAproteinwasmostfrequentlyoccurredamongstrainswithMICforciprofloxacinofmorethan4μg/ml,whereasadoublemutationatSer-83residueofgyrAandSer-80ofparCwasfoundinstrainswithMICofciprofloxacinofmorethan8μg/ml.Astothepositiveratesofclass1integron(IntI-1)andqacE△1-sul-1,itwasfoundtobe60.0%and77.5%respectively

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简介：ToinvestigatetheeffectsofoverallalkaliofatraditionalChinesemedicine“Tongbiling”(brucineandstrychninealkaloidsinmain)onthecytokinesexpressioninTh1andTh2cellsinthesynovialfluidofpatientswithrheumatismarthritisandtheirsignalpathway,themononuclearcellsinthesynovialfluid(SFMC)ofpatientswereisolatedbyFicoll-Hypaquegradientcentrifugation,andtheCD3^+CD69^+andCD3^+HLA-DRantigenwereanalyzedbyflowcytometryincomparisonwiththoseoftheperipheralblood.TherestofcellswereculturedafterresuspensionwithRPMI1640culturemedium.Phorbol12,13-dibutyrate(PDB)andionomycinwereaddedsuccessivelyintotheculturewithvariousconcentrationofoverallalkaliTongbiling(TBL).After4hofcultivation,theexpressionofIFN-γandIL-4inCD3^+cellswereanalyzedbyflowcytometry.TheinfluenceofoverallalkaliTBL(100mg/I,)ontheintracellularcalciumwasinvestigatedafterFluo-3/AMlabelingandstimulationwithPDBandionomycinat1,2,4and10min,andtheinfluenceofTBLontheexpressionofCD3^+CD69^+cellsweredeterminedwithstimulationofPDBfor24hinthewholebloodlymphocytesculture.ItwasfoundthatthepercentageofTcellsbearingCD69wassignificantlyup-regulated(77%),whilethatofTcellsbearingHLA-DRwas44%inthesynovialmononucleatedcells.AfterPDBandionomycinstimulation,theexpressionofIFN-7inCD3~cellswereup-regulated,buttherewasnochangeontheexpressionofIL-4inCD3^+cells,indicatingthatratioofTh1/Th2wassignificantlyincreasedandThcellsdifferentiatetoThlcellsinmainly.FourconcentrationsofoverallalkaloidofTBI,(200mg/L,100mg/L,50mg/L,25mg/L)coulddown-regulatedtheexpressionofIFN-γinCD3^+cellsandtheTh1/Th2ratioobviously,butalltheconcentrationsoftheoverallalkaloidshadnoeffectontheexpressionofIL-4inCD3^+cells.100mg/Lconcentrationoftheoverallalkaloiddidnotdown-regulatetheintracellularcalciumlevel.Eachconc