简介：核是最突出的subnuclear结构，它在真核细胞的细胞的进程执行许多函数。以便在牛的房间理解nucleoli的结构、功能的角色，我们分析了牛的nucleoli的proteomic作文。nucleoli从Madin钞票牛的肾房间被孤立并且由SDS页和强壮的阳离子交换层析在分别以后由LC-MS/MS使分析遭到了到proteomic。用搜索和GPM数据库寻找的吉祥物数据库的数据的分析在牛的nucleoli识别了311蛋白质，它以前包含了22蛋白质没在人的nucleoli的proteomic分析识别。用GoMiner软件的识别蛋白质的分析建议牛的nucleoli包含了涉及ribosomal生物的续生说的蛋白质，房间周期控制，transcriptional，翻译并且translational以后规定，运输，和结构的组织。

简介：这研究的目的是评估crosslinking效果一自然地bioprosthetic心阀门的材料上的crosslinkingreagent-procyanidins(PA)。在由procyanidins修理牛的心囊的纸巾以后，crosslikng特征，机械性质，在vitro，酶的降级抵抗，hydrophilicity和溶血测试被检验。结果证明有glutaraldehyde(GA)或procyanidins的生物织物的固定象在vitro一样增加了它的变性作用温度，表面hydrophilicity和机械性质酶的降级抵抗。在变性作用温度没有重要差别，机械性质，hydrophilicity并且在里面在glutaraldehyde和procyanidins之间的酶的降级修理了的vitro纸巾。然而，procyanidins的最终的张力的力量修理了纸巾比glutaraldehyde显著地优异固定纸巾。溶血测试证明procyanidins的那溶血率修理了纸巾比glutaraldehyde的修理纸巾的低。这研究显示出那个procyanidins罐头交叉连接它牛的pericardiaa有效地没有毒性。我们的结果建议这个方法可能是为bioprosthetic心阀门的准备的一条有用途径。

简介：

简介：客观：到为在非细胞组成的牛的心包的准备的最好的过程的搜索，为构造织物工程方法提供脚手架给那么:牛的心包与5个方法被对待，它被划分成6groups.GroupA:新鲜bovinepericardium；GroupB:Trypsin-detergentgroup；GroupC:Freeze-thaw-detergent24h组；组D:.Freeze-thaw-detergent48h组；组E:Freeze-thaw-nuclease组；组F:净化核酸酶的group.Then，由他观察th的染色并且扫描的电子显微镜

简介：Themonoclonalantibodyagainstbovinerotavirus(BRV)receptor(BRV-R-mAb)wasusedtoexplorethesimilaritybetweenthereceptorsofBRVandhumanrotavirus(HRV).ELISA,dotimmunobindingassay,cellprotectionassay,solid-phaseassayandimmunohistochemistrymethodwereapplied.BRV-R-mAbboundbothanti-BRVIgGandanti-HRVIgGrespectivelyandcouldprotectMA104cellsagainstBRVandHRVchallenges.Immunohistochemistrytestshowedthattherewererotavirusreceptorsonthesurfacesoffoetalintestinal,trachealmucosaandMA104cellsmembrane.WepurifiedtherotavirusreceptorsonMA104ceils,whichcouldbindbothBRVandHRVinvitro.ItisconcludedthatBRVreceptorandHRVreceptorarehomogenousproteinsandcanberecognizedbybothBRVandHRV.

简介：学习使用了自然地发生的crosslinkingreagent-genipin化学上修改非细胞组成的牛的心包，准备设计支架材料，和评估genipincrosslinked的心脏的阀门织物由调查纸巾的物理、化学的性质的牛的心包的非细胞组成的矩阵例如表面性质，crosslinking特征，机械性质，抵抗到在vitro的酶的能力，和溶血测试。结果证明有genipin的非细胞组成的牛的心包矩阵crosslinked是强壮的hydrophilicity，高crosslinking索引，和稳定的结构，它能维持好机械性质。作为为阀门织物工程的一种支架材料，它有宽申请前景。

简介：Toinvestigatetheeffectofphotodynamictherapy(PDT)withhematoporphrinmonomethylether(HMME)onbovineimmunodeficiencyvirus(BIV)canprovidethebasistheoryforphotoinactivationofhumanimmunodeficiencyvirus(HIV).ToassesstheprotectionofHMME-PDTonthecelllineCf2ThinfectedwithBIVR29by3-(4,5)-dimethylthiahiazol-2-yl-3,5-di-phenytetrazoliumbromide(MTT)withpowerdensityof5and25mW/cm~2andenergydensityfrom0.6to3J/cm~2.ToobservetheinhibitionofmembranefusionusinganewreportercelllineBIVEbyfluorescencemicroscope.HMME-PDThassignificantprotectanteffectsonCf2Th-BIVR29withbothpowerdensities,especiallyinthegroupofhighpowerdensity.FluorescentmicroscopeshowsthatthereisnosignificantdifferencebetweenthegroupofPDTandcontrol,whichmeansPDTcouldnotinhibittheBIV-mediatedmembranefusion.

简介：有在15和37污慫楬敮猠汯瑵潩的牛的浆液白朊(BSA)的gatifloxacin(HGA)的相互作用？

简介：Anewthermokineticreducedextentmethodforstudyingofthereversiblecompetitiveinhibitionofsinglesubstrateenzyme-catalyzedreactionswasproposedinthispaper.Thereactionthatarginase-catalyzedhydrolysisofL-argininetoL-ornithineandureaandtheinhibitionofthisreactionbytheproduct,L-ornithine,andexogenousL-lysinewerestudiedat37℃in40mmol·L^-1sodiumbarbiturate-HC1buffersolution(pH=9.4).MichealisconstantKmforarginineandmaximumvelocityVmofthereactionweredeterminedtobe5.14mmol·L^-1and1.13×10^-2mmol·L^+1·s^-1,respectively.TheproductinhibitionconstantKpandinhibitoryconstantK1ofL-lysineweredeterminedtobe1.18and5.6mmol.L-l,respectively.Alltheresultshavebetterrepeatabilityandself-consistencyandareinagreementwithliteraturevalues.Thisnewmethodusingmoredirectthermalinformationfromtheprocesswouldgivemorereliablekineticinformationthanthetraditionalinitialratemethod.

简介：

简介：

简介：Thebovinehydroxyapatite(BHA)wasappliedtopreparebiologicaltissueengineeringscaffoldsbythemethodofextrusionfreeforming.Toachievethisgoal,BHAwereaddedtosodiumalginate(SA)solutiontoformaslurrysysteminappropriateproportion.Theresultingmixtureswerefabricatedtobeakindofcontrollableandporousscaffoldsfollowedwithcross-linkingin5%calciumchloride(CaCl2)solutionfor24h.Afterthat,thescaffoldsweresinteredinairat1000,1100,1200and1300℃for5h.Scanningelectronmicroscopy(SEM)andX-raydiffraction(XRD)studieswereperformedonthescaffoldstoanalyzeitsmicrostructureandconstituent.Toexploretheeffectofsinteringtemperatureonscaffolds,thecompressivestrength,volumeshrinkageandwaterabsorptivityofBHA-SAcompositescaffoldsaftersinteringwereinvestigated.TheresearchtestsindicatedthefeasibilityofapplyingBHApowderto3Dprinting.Besides,thescaffoldssinteredinarespectivelylowertemperaturepossessmuchmoreporesandperformedhigherwaterabsorptivity,whichmeansbettercellularaffinity.Andscaffoldssinteredbetween1100and1200℃presentshighercompressivestrength.

简介：

简介：在这研究，数字图象关联(DIC)被采用评估牛的蹄角的机械性质。用特定的过程，张力的标本地区包括了4脚底和5从前面的爪和一头成熟黑安格斯奶牛的后面的蹄被切除。在抽取以后，标本被调节与任何一个0%的相对潮湿内容获得角(干燥)或100%(湿)然后使遭到了到单轴的紧张到失败。所有“干燥”、“湿”的蹄角的平均有弹性的模量(E)是2870瑵漠?潦吗？

简介：

简介：客观：在牛的心包的非细胞组成的新鲜标本在endothelial细胞(Ecs)的粘附和增长上调查脉管的endothelial生长因素(VEGF)和它的效果的绑定和版本，它被heparinization.Methods修改:牛的心包的Cross-linked非细胞组成的新鲜标本是由三个方法的heparinized:(1)heparinizedN-(3-dimethylaminopropyl)-N'-ethylcarbodiimidehydrochloride(EDC)对待非细胞组成的织物样品；(2)(ethyleneimine)(PEI)heparinizedpoly对待

简介：Thisstudyexaminestheeffectsofxenograftsofmicroencapsulatedbovinechromaffincells(BCCs)ontherotationalbehaviorofhemiparkinsonianmonkeyrecipients.Inaddition,itdeterminesthecontentofmonoamineneurotransmittersandtheirmajormetabolitesintheneostriatuminhemiparkinsonianmonkeys.Thehemiparkinsonianmodelinmonkeyswasinducedbyaunilateralintracarotidinjectionofmethyl-phenyl-tetrahydropyridine(MPTP).UnencapsulatedBCCs(n=2),BCCsmicroencapsulated(n=6)inalginate-polylysine-alginate(APA)membranesaswellasemptymicroencapsules(n=1)weregraftedintotheneostriatumofthehemiparkinsonianmonkeys.

简介：按照一定的比例，在小牛血清的水溶液中加入丙酮和乙醇，然后在加热的情况下可形成稳定的小于５００ｎｍ的微球胶体溶液。这种方法产生的微球可在较低温度（７０～７５℃）和较短时间（２０分钟）内达到稳定，比较传统的高温（１００℃以上）和长时间（３０分钟以上）的加热制备法，这种丙酮乙醇加热变性技术有许多优点。这篇文章描述了制备过程的工艺条件，如丙酮、乙醇和小牛血清比例；加热时间和加热温度对微球形成以及对四氯四碘荧光素包裹率的影响。由本方法产生的微球可包裹４０％左右的四氯四碘荧光素。在３７℃和ＰＢＳ溶液中，上述微胶囊在１５天时间里释放了起始四氯四碘荧光素包含量的２５～６０％。

简介：UV-inducedgraftpolymerizationofacrylicacid(AA)onpoly(etheretherketone)(PEEK)filmswascarriedouttointroduce―COOHforthesubsequentimmobilizationofbovineserumalbumin(BSA).BSAwasintroducedonPEEKsurfacebasedonthecondensationreactionbetween―NH2and―COOH.Themodifiedsurface(PEEK-BSA)wascharacterizedbyenergy-dispersespectrometry(EDS),X-rayphotoelectronspectroscopy(XPS),watercontactanglemeasurementandUVspectrumanalysis.Thecontactanglewasfoundtodecreasefrom104°forthevirginPEEKfilmsto63°fortheBSA-immobilizedPEEKfilms,demonstratingasignificantimprovementofsurfacehydrophilicity.Moreover,theappearanceofnitrogenonPEEKfilmconfirmedbyXPSandEDSindicatestheimmobilizationofBSAonPEEKsurface.

简介：