简介：Inordertocreateanewmimicofglutathioneperoxidase(GPx),bioimprintingwasusedtogenerategluta-thione(GSH)bindingsiteandchemicalmodificationwasusedtoincorporatecatalyticgroupselenocystine(Sec).Humanserumalbumin(HSA)andS-substituteddinitrophenylglutathione(GSH-S-DNP)werechosenastheimprintedmatrixandimprintingtemplate,respectively,togenerateaGSH-imprintedprotein(GSH-HSA)bybioimprinting.SecwasincorporatedintotheGSH-HSAbychemicalmodificationtogiveanewGPxmimic(Se-GSH-HSA).Se-GSH-HSAdisplayedconsiderablyhigherGPxactivitythannon-printedHSA(Se-HSA).TheenzymicpropertiesandkineticsofSe-GSH-HSAwerestudied.Moreover,Se-GSH-HSAwasconfirmedtohavestrongerantioxidantabilitytoprotectmitochondriaagainstoxidativedamagewithferroussulfate/ascorbate-inducedmitochondriadamagemodel,indicatingthatSe-GSH-HSAhaspotentialapplicationinmedicine.

简介：有在15和37污慫楬敮猠汯瑵潩的牛的浆液白朊(BSA)的gatifloxacin(HGA)的相互作用？

简介：在这个工作，我们通过腐蚀测试在纯镁的腐蚀上在氯化物离子和白朊的synergistic效果上报导。我们证明白朊的吸附主要在NaCl答案影响纯镁的阳极的极化行为。白朊的低集中在起始的沉浸时间提高纯镁的反应反应和氢的起始的发展。1g/L白朊的增加在NaCl答案为纯镁提供有限腐蚀控制。与低集中白朊比较，10g/L白朊的增加能有效地与0.20.8wt.%的NaCl集中在测试答案禁止纯镁的进一步的溶解，但是这效果与增加氯化物离子的集中逐渐地降低。

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简介：ObjectivesTodetectwhetherpersistingortransientglucosemetabolismdisorderisresponsibleforadmissionhyperglycemiainpatientswithacutemyocardicinfarction(AMI).MethodsTwogroupsofpatientswereenrolled:AMIgroupandcontrolgroup.Fastingplasmaglucose,2hoursplasmaglucose,glycatedalbumin(GA)andglycatedhaemoglobin(HbA1c)weremeasuredatbaselineinbothgroupsand30daysafterAMIattackinAMIgroup.Results(1)Therewerenosignificantdifferencesinbaselinecharacteristicsbetweenbothgroups;(2)Comparedwiththecontrolgroup,thelevelsofGAandHbAlcinAMIgroupatbaselineweresignificantlyhigher.(3)At30dayfollow-upinAMIgroup,bothFBGand2hPGdecreasedtonormalvalues,HbAlcdidnotchange,butonlyGAkeptonincreasing.ConclusionsHyperglycemiaonadmissioninpatientswithAMIresultedfrombothpreexistingmetabolicdisorderandstressreactionaswell.GAistheonlyindicatorthatcouldrecalltheexaggerationofglucosemetabolicdisorderduringAMIattackat30dayfollow-up.

简介：按照一定的比例，在小牛血清的水溶液中加入丙酮和乙醇，然后在加热的情况下可形成稳定的小于５００ｎｍ的微球胶体溶液。这种方法产生的微球可在较低温度（７０～７５℃）和较短时间（２０分钟）内达到稳定，比较传统的高温（１００℃以上）和长时间（３０分钟以上）的加热制备法，这种丙酮乙醇加热变性技术有许多优点。这篇文章描述了制备过程的工艺条件，如丙酮、乙醇和小牛血清比例；加热时间和加热温度对微球形成以及对四氯四碘荧光素包裹率的影响。由本方法产生的微球可包裹４０％左右的四氯四碘荧光素。在３７℃和ＰＢＳ溶液中，上述微胶囊在１５天时间里释放了起始四氯四碘荧光素包含量的２５～６０％。

简介：UV-inducedgraftpolymerizationofacrylicacid(AA)onpoly(etheretherketone)(PEEK)filmswascarriedouttointroduce―COOHforthesubsequentimmobilizationofbovineserumalbumin(BSA).BSAwasintroducedonPEEKsurfacebasedonthecondensationreactionbetween―NH2and―COOH.Themodifiedsurface(PEEK-BSA)wascharacterizedbyenergy-dispersespectrometry(EDS),X-rayphotoelectronspectroscopy(XPS),watercontactanglemeasurementandUVspectrumanalysis.Thecontactanglewasfoundtodecreasefrom104°forthevirginPEEKfilmsto63°fortheBSA-immobilizedPEEKfilms,demonstratingasignificantimprovementofsurfacehydrophilicity.Moreover,theappearanceofnitrogenonPEEKfilmconfirmedbyXPSandEDSindicatestheimmobilizationofBSAonPEEKsurface.

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简介：Theadaptivetreatmenttolerance(ATT)ofcancercellsisthemainencumbrancetocancerchemotherapy.Apotentialsolutiontothisproblemistotreatcancercellswithmultipledrugsusingnanoparticles(NPs).Inthisstudy,wetestedtheco-administrationofcurcumin(Cur)anddoxorubicin(Dox)toMCF-7resistantbreastcancercellstoblocktheATTandelicitefficientcellkilling.Drugswereco-administeredtocellsbothsequentiallyandsimultaneously.Sequentialdrugco-administrationwascarriedoutbypre-treatingthecellswithalbuminnanoparticles(ANPs)loadedw让hCur(Cur@ANPs)followedbytreatmentwithDox-loadedANPs(Dox@ANPs).Simultaneousdrugco-administrationwascarriedoutbytreatingthecellswithANPsloadedwithboththedrugs(Cur/Dox@ANPs).Wefoundthatthesimultaneousdrugco-administrationledtoagreaterintra-cellularaccumulationofDoxandcellkillingwithrespecttothesequentialdrugco-administration.However;thesimultaneousdrugco-administrationledtoalowerintracellularaccumulationofCurwithrespecttothesequentialdrugco-administration.WeshowedthatthisresultwasduetotheaggregationandentrapmentofCurinthelysosomesassoonasitwasreleasedfromCur@ANPs,aphenomenoncalledlysosomotropism.Incontrast,thesimultaneousreleaseofDoxandCurfromCur/Dox@ANPsintothelysosomesledtolysosomalpHelevation,which,inturn,avoidedCuraggregation,ledtolysosomeswellinganddrugreleaseinthecytosol,andfinallyprovokedefficientcellkilling.Ourstudyshedthelightonthemolecularprocessesdrivingthetherapeuticeffectsofanti-cancerdrugsco-administeredtocancercellsindifferentmanners.

简介：BiochaninA(BCA),themostabundantisoflavoneinchickpeas,presentsawiderangeofbiologicalactivities,suchashypolipidaemic,anti-oxidative,antiproliferative,andestrogen-likeeffects.WeinvestigatedtheinteractionbetweenBCAandhumanserumalbumin(HSA)viaseveraltechniques.UV–VisabsorptionspectroscopyverifiedtheconformationalvariationofHSAafterBCAaddition,andfluorescencespectroscopyrevealedtherelevantbindingparameters.CirculardichroismspectroscopywasusedtoestimatethesecondarystructuralchangesofHSAwithandwithoutBCA.MoleculardockinganddynamicssimulationswerethenappliedtostudythecharacteristicsofHSAwithBCA.EnergydecompositionanalysiswasusedtoprovethatTrp214insubdomainIIAofHSAisthemostlikelybindingsiteofBCA.VanderWaalsforcesandhydrophobicinteractionsmayplayimportantrolesduringthebindingprocess.AllofourresultsshowedthatBCApresentssignificantbindingaffinitytoHSA,thusconfirmingthattheroleofHSAhasasanefficienttransporterofbiomolecules.

简介：AbstractAlbumin solutions derived from human plasma have demonstrated clinical benefits as intravenous fluid therapy in clinical settings such as liver disease, sepsis, intensive care, and surgery. For all plasma-derived medicinal products, there is a potential risk from pathogens, including relevant blood-borne viruses, emerging viruses, and prion proteins. To minimize the risk of transmissible infections, the production of human albumin solutions includes rigorous donor selection and plasma testing, and effective pathogen removal and inactivation methods such as fractionation and pasteurization. Compliance with international pharmacopeial standards for purity and prekallikrein activator and aluminum content is crucial, as is post-marketing pharmacovigilance for the continuous monitoring of adverse events. This review focuses on the effectiveness of manufacturing methods in the production of plasma-derived albumin, to ensure the safety of hyperoncotic solutions for volume expansion. We evaluated evidence identified through online database (PubMed) searches and from unpublished sources, on the manufacturing and pathogen safety of plasma-derived albumin solutions. The results confirmed the already established and evolving pathogen reduction capacity of the reviewed manufacturing methods. Up-to-date post-marketing pharmacovigilance data and log10 reduction factors for known and emerging pathogens during albumin production are included. Towards the goal of ever-increasing clinical safety, potential areas of improvement, such as compliance rates for the completion of donor health questionnaires, are also discussed. Taken together, the current manufacturing and pathogen reduction steps result in albumin products of greater purity than previous-generation products, with a high margin of pathogen safety against known and emerging pathogens, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

简介：ThispaperreportstheadsorptionofBovineSerumAlbumin(BSA)ontoDielectricBarrierDischarge(DBD)processedPoly(methylmethacrylate)(PMMA)surfacesbyaQuartzCrystalMicrobalancewithDissipationmonitoring(QCM-D)tech-nique.ThepurposeistostudytheinfluenceofDBDprocessingonthenatureandscaleofBSAadsorptiononPMMAsurfaceinvitro.ItwasobservedthatDBDprocessingimprovesthesurfacewettabilityofPMMAfilm,afactattributabletothechangesinsurfacechemistryandtopography.ExposureofthePMMAtoPhosphateBuffedSaline(PBS)solutionintheQCM-Dsystemresultedinsurfaceadsorptionwhichreachesanequilibriumafterabout30minutesforpristinePMMA,and90minutesforprocessedPMMAsurface.SubsequentinjectionofBSAinPBSindicatedthattheproteinisimmediatelyadsorbedontothePMMAsurface.ItwasrevealedthatadsorptionbehaviourofBSAonpristinePMMAdiffersfromthatonprocessedPMMAsurface.AsloweradsorptionkineticswasobservedforpristinePMMAsurface,whilstaquickadsorptionkineticsforprocessedPMMA.Moreover,thedissipationshiftofproteinadsorptionsuggestedthatBSAformsamorerigidstructureonpristinePMMAsurfacethatonprocessedsurface.ThesedatasuggestthatchangesinwettabilityandattendantchemicalpropertiesandsurfacetextureofthePMMAsurfacemayplayasignificantroleinBSAadsorptionprocess.

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简介：AbstractBackground:Time in range (TIR) refers to the time an individual spends within their target glucose range, which now has been popularized as an important metric to classify glycemic management and also recognized as an important outcome of current diabetes therapies. This study aimed to investigate the association between TIR and the severity of the urinary albumin excretion rate (UAER) in patients with type 2 diabetes mellitus (T2DM).Methods:We retrospectively analyzed the data of 1014 inpatients with T2DM at the Department of Endocrinology and Metabolism of Peking University International Hospital, China. TIR was defined as the percentage of blood glucose within the target range of 3.90-10.00 mmol/L. Urine samples for assessment of UAER were collected for 3 consecutive days from the start of hospitalization.Results:The TIR values for patients with normal urine levels of albumin, microalbuminuria, and macroalbuminuria were 70% ± 20%, 50% ± 20%, and 30% ± 20%, respectively (all P < 0.001). The patients were stratified according to quartiles of TIR as follows: quartile (Q) 1, <55%; Q2, 55%-72%; Q3, 73%-83%; and Q4, >83%. The incidences of microalbuminuria in Q1, Q2, Q3, and Q4 were 41.1%, 21.6%, 7.1%, and 5.5% (all P < 0.001), respectively. The respective incidences of macroalbuminuria were 24.2%, 1.1%, 1.4%, and 0% (all P < 0.001). In multinomial logistic regression analyses, TIR was significantly correlated with microalbuminuria (odds ratio [OR] 0.58, 95% confidence interval [CI]: 0.52-0.65, P < 0.001) and macroalbuminuria (OR 0.26, 95% CI: 0.18-0.38, P < 0.001) after adjusting for age, sex, body mass index, diabetes duration, systolic blood pressure, and levels of triglycerides, glycosylated hemoglobin A1c, and creatinine.Conclusion:The proportion of blood glucose in TIR is closely related to the severity of UAER in patients with T2DM.

简介：AbstractObjective:Glioma is the most common and aggressive primary brain tumor. Here, we aimed to establish a nano-drug carrier system to traverse the blood-brain barrier for the treatment and inhibition of glioma migration.Methods:The synthesis of bovine serum albumin protected-silver nanoclusters (BSA-AgNCs) was performed using chemical reduction. The drug paclitaxel (PTX) can be loaded into BSA-AgNCs through electrostatic and hydrophobic interactions to formulate spherical BSA-AgNC-PTX nanoparticles (BSA-AgNC-PTX NPs). A glioma mouse model was established by injecting U251-GFP-Luc cells into the mouse striatum, and all surgical procedures were approved by the Animal Ethics Committee of Nanchang University (SYXK2019-0003) on December 29, 2019.Results:The BSA-AgNC-PTX NPs were able to efficiently pass through the blood-brain barrier, both in vitro and in vivo, to deliver the drug to the tumor site. The in vivo assessment of BSA-AgNC-PTX NPs in glioblastoma multiforme-bearing mice revealed the significant inhibition of tumor growth and migration, prolonging the survival of the mice.Conclusion:These results indicated that BSA-AgNCs might represent an ideal nanocarrier for the treatment of glioma and has the potential to be used in the treatment of a variety of central nervous system diseases.