简介:目的:用荧光定量聚合酶链反应(FQ-PCR)法准确地定是检验血清中乙型肝炎病毒(HBV)的数量和免疫指标,以指导临床。方法:采用HBV-DVAFQ-PCR诊断试剂盒,以一种完全闭管式的PCR和荧光探针杂交技术组合所产生的实时检验定量PCR方法,检测了58例临床血清标本。结果:经FQ-PCR检测,9例HBV的HBsAg、HBeAg、HBcAg、HBcAb都阳性标本,其HBV-DNA的阳性率为100%,平均HBV-DNA拷贝数为2.0×10^8/ml;16例HBsAg、HBeAb、HBcAb都阳性的标本,其阳性率为62.5%(10例),平均拷贝数为5.2×10^5/ml,而常规PCR只有33%的阳性率;7例HBsAb、HBeAb、HBcAb都阳性的标本,其阳性率为14.3%(1例),平均拷贝数为4.7×10^3ml。结论:HBV-DNA的FQ-PCR检测较常规PCR更具有较高的灵敏度和特异性,且定量准确,结果可靠,能够避免PCR后处理导致物假阳性污染,可以真实反映HBV的感染和低复制状态,对于乙型肝炎的临床诊断,治病方案的选择和疗效观察有较大的指导意义。
简介:AdoublehelixmodelofchargetransportinDNAmoleculeisgivenandthetransmissionspectraoffourDNAsequencesareobtained.ThecalculatedresultsshowthatthetransmissioncharacteristicsofDNAarenotonlyrelatedtothelongitudinaltransportbutalsotothetransversetransportofmolecule.Theperiodicsequencewiththesamecompositionhasstrongerconductionability.Withtheincreasingofbasescomposition,theconductiveabilityreduces,buttheweightofθdirectionrisesinchargetransfer.
简介:The2004SoutheastAsiaTsunamikillednearly5,400peopleinSouthernThailand,includingforeigntouristsandlocalresidents.TorecoverDNAevidenceasmuchaspossiblefromtheseriouslydecomposedbodies,weexploredproceduresofsamplepreparationfrombothboneandtoothsamplesaswellasbothmitochondrialandnuclearmarkers.DespitehavingfailedtorecoverenoughDNAfornuclearmarkertyping,wesucceededinobtainingfullyinformativeresultsformitochondrialmarkers(HV1andHV2)from258toothsampleswithasuccessrateof51%(258/507).UsinganorganicDNAextractionmethodcoupledwithanultrafiltrationstep,weobtained16STR(including13CODISloci,onesexdiscriminationlocus,andtwoIdentifilerloci)profilesfor834sampleswithasuccessrateof79%(834/1,062).Inaddition,bycomparingtheallelicfrequenciesbetweenthetypedsamplesasagroupandotherindexpopulations,weconcludethattheThaitsunamivictimsareacombinedgroupofseveralpopulations.Ourresultsprovidevaluableevidenceandprotocolsforthefutureforensicpractice.
简介:Objective:TolookforthefurtherevidenceforHPVL1HPV16E6,HPV18E6andEBVascarcinogenicfactorsinlaryngealcarcinoma.Method:weexaminedrepresentativenumbersofspecimensfromlaryngealcancerwithhighlysensitivePCRtechniqueforthepresenceofHPVL1andhigh-risktypesHPV16E6,HPV18E6andEBVLMP1.Results:UsingPCRdetection,7.3%sampleswereHPVL1positive,52.03%wereHPV16E6positive,30.89%wereHPV18E6positiveand9.13%wereEBVLMP1positive.ThelowincidenceofHPVL1andhighincidenceofHPV-16E6andHPV18E6genessuggestthatHPVmightbeintegratedintotumorcells.OurresultssupportaroleofHPV-16andHPV-18infectioninthepathogenesisoflaryngealcarcinomainChina.Conclusion:IntegrationofE6intohostgenomeandstableexpressionofthesegenesmaybeassociatedwiththecarcinogenesisoflaryngealcarcinoma.HPV-16andHPV-18maysynergisticallyfunctiononthepathogenesisoflaryngealcarcinoma.Ourresultssuggestanassociationoflaryngealcarcinogenesisandinfectionwiththehigh-riskHPVtypes16,HPV18andEBV.
简介:摘要目的探讨用女性尿液检测沙眼衣原体(chlamydia trachomatis,CT)DNA和淋病奈瑟菌(neisseria gonorrhoeae,NG)DNA的临床意义。方法随机选取2019年1—6月性病科女性患者212例,每位患者取宫颈拭子做CT-DNA、NG-DNA、淋球菌培养,尿液做CT-DNA、NG-DNA。同时设置对照组30例。结果宫颈拭子CT-DNA阳性率为18.40%(39/212);尿液CT-DNA阳性率为19.81%(42/212),二者阳性率比较差异无统计学意义(χ2=0.14,P=0.71);宫颈拭子CT-DNA和尿液CT-DNA检测结果一致性好(Kappa=0.92,P<0.01)。淋球菌培养阳性率为5.19%(11/212),宫颈拭子NG-DNA阳性率为12.26%(26/212),二者阳性率比较差异有统计学意义(χ2=6.662,P=0.010);尿液NG-DNA阳性率为10.38%(22/212),与淋球菌培养阳性率相比,差异有统计学意义(χ2=3.976,P=0.046);宫颈拭子NG-DNA阳性率与尿液NG-DNA阳性率相比,差异无统计学意义(χ2=0.376,P=0.540)。宫颈拭子NG-DNA与尿液NG-DNA检测结果一致性好(Kappa=0.86,P<0.01)。结论用女性尿液检测CT-DNA、NG-DNA敏感性高、特异性好,取材方便,建议在临床普及。