简介：

简介：

简介：Byscanningandtransmissionelectronmicroscopy,leukemlccelbwereobwrvedafterCFU-Mixculture.Eventhoughgranulocytlcgrowthfactor,erythropoietinandlymphocytlcgrowthfactorwereaddedatvitor,acuteleukemlccelbstillshoweddefectsIndifferentiationandmaturation.Thesewerecharacterizedbyabnormalcolonywhichconsistedofsmoothcells,bizarreshape,nuclear-cytoplasmicasynchronyIndevelopment,andappearanceofnuclearbleb.However,chronicmyelogenousleukemlccelbweremorenaturethantheacuteones,manifestinginnormalcolonywithfinger-likeprojectionsandruffledmembrane.Macrophagesandeosinophilscouldbeobserved.ItbsuggestedthattherebadifferenceIndifferentiationbetweenacuteandchronicleukemiacells.

简介：Objective:Todetectsuperoxideanion(O-2)signalsintheheart,liver,lungandkidneytissuesafterburns.Methods:Twenty-fourmaleratswererandomizedinto4groups.Theratsofexperimentalgroupswereimmersedin100℃waterfor15secondsand25%third-degreeburnwascreated.Thoracotomyorlaparotomywasperformedat5,10,15,20and30minutesafterburn,andspecimensoftheheart,lung,liverkidneywereobtainedforburnedrats.Thespecimenswerethenpreservedinliquidnitrogenforcryo-preservationanddetectedimmediatelyusingEPR.Results:ThesignalsofsuperoxideOradicalappearedintheheart,lung,liverandkidneyspecimens10-15minutesafterburn.Conclusions:Thereisadirectevidenceofoxygenfreeradicals(OFRs)injurytovisceraofburnedratsbetween10-15minutesafterburn.

简介：Forelectronicmicroscopicobservation,wefoundSSV-transformedNIH3T3cellsweredifferentfromnon-transformedcells.InSSV-transformedNIH3T3cellsnucleicytoplasmaratiowasincreasedandincytoplasmatheribosomes(polyribosomeswereattachedtotheswollenroughendoplasmicreticulum.Itwaslikelythatribosomeswerelinedtogetherfunctionallyandstructionallytoproducespecificprotein(PDGF-likeprotein).

简介：ObjectivesTocomparethedifferentpatternsofcoronaryarterycalcification(CAC)inacuteandchroniccoronarysyndromesutilizingelectronbeamcomputedtomography.BackgroundPathologic,angiographic,andintracoronaryultrasoundstudiesrevealedsharpdifferencesintheplaquemorphologybetweenpatientswithacuteversuschroniccoronarysyndromes.Whethertherearedifferentpatternsofcoronaryarterycalcificationinpatientswithstableanginapectoris(SAP)andinthosewithunstableanginapectoris(UAP)oracutemyocardialinfarction(AMI),however,remainsunclear.MethodsTwohundredandsixty-fourpatients,including67withSAP,94withUAPand103withAMIwerestudiedwithelectronbeamcomputedtomographyforanalysisofcoronaryarterycalcificationandwithselectivecoronaryangiographyfordeterminationofatheroscleroticlesions.CACprevalenceandcalciumscorewerecomparedamongthreegroups,andrelationofCACtotheseverityofatheroscleroticdiseaseintheSAPandUAP

简介：Objective:Tofurtherinvestigatetheosteogenicpotentialofrabbitmarrowstromalstemcellsculturedinvitro.Methods:Rabbitmarrowstromalstemcellswereisolatedbydensitygradientcentrifugationmethodandamplifiedintheflasks,usingtheosteogenicinducingconditions(OGC)astheculturemedia.Theosteogenicpotentialofmarrowstromalstemcellswereinvestigatedbymeansofbone-seekingfluorescenc(tetracycline)labeling,AlizarinredS(ARS)staining,Alcianblue-Siriusred(AS)staining,andscanningelectronmicroscope.Results:Afterbeingpassaged,themarrowstromalstemcellsincreasedinnumber,becameconfluentandformedmulti-layerstructure.Thestromalstemcellsexcretedinnumerabletinygranules,heapinguponthecellbodyandmerginggraduallyintofoggysubstances.Thesefoggysubstanceskeptonenlargingandformedround,oval,orflake-likenodules.Thesenodulesrevealedbrightgoldenyellowfluorescenceunderfluorescencemicroscopewhenlabeledwithtetracycline.HistochemicalstudywithspecificnewbonestainingwithARSrevealedpositivecalciumreaction,bothdenotingthattheywerenewlyformedbonetissues.AftertheywerestainedwithAS,collagenandacidmucopolysaccharidewereshown.Underscanningelectronmicroscope,threetypesofcellswithdifferentconfigurationswerefound.Theywereglobularcells,spindle-shapedcellsandpolygonalorpolygonalcells.Granuleswereexcretedfromthecellsandheapeduponthecellbody.Needle-shapedandirregularlyrectangularcrystalsalsoappearedandagglomeratedwiththegranulestoformnodulesandtrabecula-likeorflake-likestructures.Conclusions:Sequenceofeventsofboneformationbyrabbitmarrowstromalstemcellsculturedinvitroisfullydepictedandconfirmed,whichprovidesthefoundationforfurtherinvestigatingthemechanismsofosteoblastdifferentiationfrommarrowstromalstemcellsandthepossibleapplicationinorthopaedics.

简介：客观:在这研究，我们试着由把蒙特卡罗模拟方法与粒子相结合建立一个起始的电子横梁模型为BJ-6的单个6MVX光检查加速波导的动态计算(TRSV)医药线性加速器。方法和Materials:1。我们改编了北京医药设备研究所(BMEI)为这研究作为放射系统做的BJ-6医药线性加速器的处理头配置。2。使用粒子动力学计算代码叫了TRSV逐出精力光谱，空间紧张分发，和横梁发生角度的起始的电子横梁参数。3。分析PDDc的6MVX光检查横梁特征，在由使用蒙特卡罗模拟的一个水幽灵的OARc(BEAMnrc，DOSXYZnrc)为被TRSV决定了的起始的电子横梁参数的预设，做PDDm的测量结果的比较，在一个真实的水幽灵的OARm，然后使用计算、测量的结果的偏差遇见错误稍微来回修改起始的电子横梁模型直到偏差不到2%。结果:在在PDDc和离开轴比率OARc的百分比深度剂量的蒙特卡罗模拟结果和在一个水幽灵的PDDm和OARm的测量结果之间的偏差在2%以内。结论:当做蒙特卡罗模拟为一台特别医药线性加速器决定一根起始的电子横梁的参数时，喜欢BJ-6，基于粒子动力学计算代码修改一些参数将给一些更多合理、更可接受的结果。