简介:BACKGROUND:Withdevelopmentsoftissueengineeringandgeneticengineering,weaimtoculturemyoblasts,whicharecharacterizedbyhighpurity,highqualityandhighproduction,forwideapplicationinneuralregenerationresearches.OBJECTIVE:Tomodifytraditionaldissociationmethodinordertoobtainmyoblasts,whicharecharacterizedbyhighpurity,highqualityandhighproduction,andexplorethebiologicalpropertiesunderinvitroculture.DESIGN:Observationalstudy.SETTING:BasicInstituteofAcademyofMilitaryMedicalSciencesofChinesePLA.MATERIALS:FourneonatalWistarratsof5daysold,bothgendersandmeanbodymassof10gwereselectedinthisstudy.Themainreagentsanddevicesweredetailedasfollows:DMEMmedium(GibcoCompany),fetusbovineserum(FBS,HycolneCompany),collagenaseⅡ(SigmaCompany),trypsin(SigmaCompany),dispaseⅡ(SigmaCompany),desminantibody(FuzhouMaixinCompany),antibodyⅡandABCkit(WuhanBosterBiotechnologyCompany),deskcentrifuge(KUBATO,Japan),andinvertedphasecontrastmicroscope(LEICADMIRB,Germany).METHODS:TheexperimentwascarriedoutintheBasicInstituteofAcademyofMilitaryMedicalSciencesofChinesePLAfromJunetoOctober2006.Neonatalratsweresacrificedundersterileconditiontoobtainskeletalmusclesoflimbs,whichwerewashedwithcoldPBS(containingbenzylpenicillinandestreptomicina),andmusculartissuewasshearedintopieces.Then,thosemuscularpieceswereaddedwithmixeddigestiveenzyme(containing2g/LcollagenaseⅡ+5g/LdispaseⅡ+0.28g/LCaCl2)astwicevolumeaspieces,dealtwithmechanicalpipettingfor5minutesandculturedinCO2incubatorfor10minutes.Theoperationwasdoneforthreetimesandthemuscularpiecesweredigestedfor45minutesintotal.Moreover,cellsweresuspendedagaininordertoobtainmyoblastsfromskeletalmuscleofneonatalrats.Inaddition,myoblastswerepurifiedwithdifferentialattachmenttechniqueandenzymedigestionsoastoobservemorpho
简介:Effectsofhydrochlorideaciddealuminationofmordenite(MOR)catalystsforthesynthesisof1-phenyl-1-xylylethane(PXE)wereinvestigated.ThestructureandacidityofcatalystswerecharacterizedbyXRD,BET,XRF,FT-IR,27AlNMRandNH3-TPDtechniques.Thecatalyticperformanceoftheacid-treatedMORzeoliteswasstudiedthroughusingthealkylationofo-xylenewithstyrene.ThetestresultsshowedthatthestrengthofremainingBr?nstedacidsitesincreaseddespitethereductionoftotalnumberofacidsitesafterdealumination,andthemicroporesofHMORwereslightlyenlargedcoupledwiththeformationofsecondarymesopores.Additionally,themodifiedHMORzeolitesshowedlongercatalystlifewiththestyreneconversionrateretained.Amongthecatalystsemployedinthisstudy,themodifiedmordenitethatwasdealuminatedbyHCl(2mol/L)couldbeusedrepeatedlywithoutsignificantlossofactivityandselectivityduringsixcatalyticruns,whichhavebeenascribedtoitsspecificacidityandstructuralproperties.
简介:UsingthestandardtruncatedPainlevexpansion,theresidualsymmetryofthe(2+1)-dimensionalmodifieddispersivewater-wavesystemislocalizedintheproperlyprolongedsystemwiththeLiepointsymmetryvector.Somedifferenttransformationinvariancesarederivedbyutilizingtheobtainedsymmetries.ThesymmetriesofthesystemarealsoderivedthroughtheClarkson-Kruskaldirectmethod,andseveraltypesofexplicitreductionsolutionsrelatetothetrigonometricorthehyperbolicfunctionsareobtained.Finally,somespecialsolitonsaredepictedfromoneofthesolutions.
简介:Anewα-fetoprotein-MIP(AFP-MIP)immunosensorbasedonglasscarbonelectrode(GCE)modifiedwithpolythionine(PTh)andgoldnanoparticles(AuNPs)wassuccessfullyfabricatedforsensitivedetectionofα-fetoprotein(AFP).Throughcontrollingelectropolymerization,A“polydopamine(PDA)-AFP”complexwasachievedapplyingAFPastemplateanddopamine(DA)asimprintedmonomers.Afterelution,thespecificcavitiescanadsorbthetargetmolecules.Usingdifferentialpulsevoltammetry(DPV)detection,thepeakcurrentdecreasedwiththeincreaseinconcentrationofAFP,andthelinearresponserangeoftheAFP-MIPimmunosensorwasfrom0.001ng/mLto800ng/mLwiththedetectionlimitaslowas0.8138pg/mL.TheMIPimmunosensorcouldbecomeanewpromisingmethodforthedetectionofAFP.Furthermore,thisMIPsensorwasdemonstratedintestingAFPinhumanserumsampleswithsatisfactoryresults.
简介:Afewadditionaldatafromourpreviousexperimentswereplottedtoemphasizetheshear-thickeningbehaviorofdeoxysickleerythrocyte(SS)suspension.Aconstitutiveequation(namedasFXequation)wasdevelopedandappliedtoacylindricalpipeflowofashear-thickeningfluid.Abluntvelocityprofileanditsvolumeflowratewerecalculated.Theflowwasnon-viscous(potential)inthecentralpartofthepipe(i.e.thecentralcoreorthecentralplug-flow),andbecamemoreandmoreviscoustowardsthewallofthepipeafteraspecificradialdistance,whichwasdeterminedbyacriticalshearrateofγF(namedasFung'sshearrate).Furthermore,combiningtheFXequationwiththeoriginalCasson'sequation,theauthorobtainedamodifiedCasson'sequationbyintroducingγF.
简介:Modifiedsensingmembranesbasedonfluorescencequenchingwerepreparedbythesol-gelmeth-od,usingformamideasthedryingcontrolchemicaladditive,tetraethoxysilaneasthemainmaterial,Ru(phen)3Cl2astheindicator.Themembranewiththeoptimumthicknessof20-50μmisuniformandcrack-free,inwhichtheindicatorhasaverysmallleakingrate.Themembraneisimmersedinwaterfor50h,themembranesensingparameterMdecreasesbylessthan5%.Thefiberopticoxygensensorwiththesensingmembranehasadetectionlimitof5×10-6M(ppm),aresponsetimeoflessthan30s,excellentreproducibilityandstability.
简介:Differentrareearth(RE=La,Ce,Pr,Nd,Sm,Y)andCumodifiednickelcatalystsforhydrogenproductionfrommethanedecompositionweresynthesizedbyasol-gelprocessandmethod.ThecatalystswerecharacterizedoranalyzedthroughBrumauer-Emmett-Teller(BET),X-raydiffraction(XRD),scanningelectronmicroscopy(SEM),transmissionelectronmicroscopy(TEM)techniques.Andthehydrogenproductionperformancewasalsoevaluatedbyafixed-bedandmicro-reactiontechniquewithCH4→C+H2asaprobereaction.Theresultsshowedthatrareearthmodificationhadplayedagreatrolefornickelcatalysts,forexample,smallernickelparticles,goodthermalstability,highactivity,etc.Lawasthebestadditiveamongrareearthmodification.TheSEMofrareearthmodifiedcatalystsshowedorderedflower-likestructureandrareearthmodificationmadethenickelparticlesmovetothesurfaceofcatalysts.Inaddition,theSEMofnano-carbonswasalsochangedbyrareearthmodificationwithlong,narrownano-carbonfibersortubesobtained.Solidcarbonformationwaspreventedbyrareearthmodification.
简介:Anewinorganic-organichybridtetravanadate[Co(2,2′-bpy)3]2V4O12·11H2O(1)hasbeenpreparedandcharacterized.X-Raydiffractionstudyrevealsthatcompound1containsclassicalclusteranions[V4O12]4–,coordinatedcations[Co(2,2′-bpy)3]2+andelevenwatermolecules,inwhichaninterestingdecamerwaterclusterisformed.Thehybridnanoparticleswerefirstlyusedasabulk-modifiertofabricateachemicallymodifiedpasteelectrode(1-CPE).Theelectrochemicalbehaviorandelectrocatalysisof1-CPEhavebeenstudiedindetail.Theresultsindicatethat1-CPEhasagoodelectrocatalyticactivitytowardthereductionofbromateina0.5mol/LH2SO4aqueoussolution.1-CPEshowsremarkablestabilitythatbeascribedtothehydrogenbondinginteractionsbetweenV4O12clusterandwatercluster,whichareveryimportantforpracticalapplicationinelectrodemodification.
简介:StructuresofaseriesofactivatedcarbonfibersweremodifiedbyimpregnatingthemwithorganicandinorganicmaterialssuchasMethyleneblue(Mb)、p-nitrophenol(PNP)、NaClorbyoxidizingwithKMnO4orHNO3.Theinfluenceofporefillingorchemicaltreatmentontheirxenonadsorptionpropertieswasstudied.TheexperimentalresultsshowthatMbandPNPfillingofactivatedcarbonfibersresultinthedecreaseofxenonadsorptioncapacitiesofthesetreatedACFs,whichisduetothedecreaseoftheirsurfaceareaandmicro-porevolume.However,theadsorptioncapacityincreasesgreatlywithoxidizingtreatmentofactivatedcarbonfibersby7mol/LHNO3.
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简介:在量的大脑图象分析,从大脑的精确大脑织物分割磁性的回声图象(MRI)是关键步。是医药图象处理的地里的最重要、困难的问题被认为。先生图象的质量被部分卷效果,噪音,和紧张不同类影响,它使分割任务变为极其挑战性。我们在场为分割和大脑先生的偏爱地修正的一个新奇模糊c工具算法(RCLFCM)想象。我们采用一个新灰色差别的系数并且设计一个新影响因素测量邻居象素的效果,以便反噪音的坚韧性能被提高。而且,我们由增加偏爱域评价模型在图象克服紧张不同类并且同时分割大脑先生图象重新定义FCM(模糊c工具)的客观函数。我们也由把象素灰色值不同与它的会员相结合构造一个新空间函数,并且充分利用在象素之间的空格信息更新会员。与由与噪音和紧张不同类的不同层次在合成先生图象上使用类似精确性的另外的最先进的途径相比,建议算法与高精确性和坚韧性产生结果到噪音。
简介:Forcompressibletwo-phasedisplacementproblem,themodifiedupwindfinitedifferencefractionalstepsschemesareputforward.Sometechniques,suchascalculusofvariations,commutativelawofmultiplicationofdifferenceoperators,decompositionofhighorderdifferenceoperators,thetheoryofpriorestimatesandtechniquesareused.OptimalorderestimatesinL2normarederivedfortheerrorintheapproximatesolution.Thismethodhasalreadybeenappliedtothenumericalsimulationofseawaterintrusionandmigration-accumulationofoilresources.
简介:Wepresentherethedevelopmentofcholesterol(Chol)-modifieddendrimersystemfortargetedchemotherapyoffolate(FA)receptor-expressingcancercells.Inourstudy,poly(amidoamine)(PAMAM)dendrimersofgeneration5(G5)werefunctionalizedstepby-stepwithChol,fluoresceinisothiocyanate(FI),andFAviaapoly(ethyleneglycol)(PEG)spacer(PEG-FA),andthenacetamidetoshieldtheirremainingsurfaceamines.ThesynthesizedG5.NHAc-Chol-FI-PEG-FA(forshort,G5-CFPF)dendrimerswereutilizedtoencapsulate10-hydroxycamptothecin(HCP),ahydrophobicanticancerdrug.WefindthateachG5-CFPFdendrimercanencapsulate13.8HCPmolecules.ThecomplexesshowaslowerreleaseprofilesofHCPinapH-dependentmannerthanthecontrolcomplexesformedusingthesamedendrimerswithoutCholunderthesameconditions.ThankstothetargetingroleplayedbyFA,thecomplexesdisplayaspecificinhibitionefficacytoFAreceptor-expressingcervicalcancercells.ThedesignedChol-modifieddendrimersmaybeadoptedasapromisingcarrierforapplicationintargetedcancertherapy.