简介：客观：当在骨头织物工程(TE)和有钛蜂窝胃的公山羊的mandible的部分缺点的修理的脚手架增强了，调查使用自然poritos的可行性。方法：有在大约50%-65%的尺寸和孔的190-230μm的一个毛孔的自然poritos被塑造进小粒的形状5公里x5公里x在尺寸的5尼姑。扩展的自体同源的公山羊的髓间充质的干细胞被recombinant导致人的形态基因的protein-2(rhBMP2)到改进osteoblastic显型。然后，髓导出造骨细胞在4x10~7/ml的密度被播种进poritos并且在培植以前在vitro孵化了48个小时。然后，osteoblasticcells/poritos建筑群被植入进mandible的缺点，缺点被钛蜂窝胃增强。poritos的培植独自扮演了著名计算机生产厂商他控制。骨头新生被估计4，8，在用roentgenographicanalysis和组织学的观察的培植以后的16个星期在16个星期以后被做。结果：新骨头能是observedhistologically在表面上并且在在在cell-seedinggroup的所有标本的自然珊瑚的毛孔，而在控制组没有在构造的中心的成骨过程的证据。结果证明新骨头接枝16个星期成功地在培植以后被恢复。结论：当与髓移植的支架材料由TE方法导出造骨细胞，这研究建议使用多孔的珊瑚的可行性。借助于钛蜂窝胃加强，mandible的缺点能成功地被恢复。它显示出在诊所为骨头缺点的重建使用这个方法的潜力。

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简介：AbstractBackground:Multiple techniques are commonly used for posterior cruciate ligament (PCL) reconstruction. However, the optimum method regarding the fixation of PCL reconstruction after PCL tears remains debatable. The purpose of this study was to compare the biomechanical properties among three different tibial fixation procedures for transtibial single-bundle PCL reconstruction.Methods:Thirty-six porcine tibias and porcine extensor tendons were randomized into three fixation study groups: the interference screw fixation (IS) group, the transtibial tubercle fixation (TTF) group, and TTF + IS group (n = 12 in each group). The structural properties of the three fixation groups were tested under cyclic loading and load-to-failure. The slippage after the cyclic loading test and the stiffness and ultimate failure load after load-to-failure testing were recorded.Results:After 1000 cycles of cyclic testing, no significant difference was observed in graft slippage among the three groups. For load-to-failure testing, the TTF + IS group showed a higher ultimate failure load than the TTF group and the IS group (876.34 ± 58.78 N vs. 660.92 ± 77.74 N [P < 0.001] vs. 556.49 ± 65.33 N [P < 0.001]). The stiffness in the TTF group was significantly lower than that in the IS group and the TTF + IS group (92.77 ± 20.16 N/mm in the TTF group vs. 120.27 ± 15.66 N/m in the IS group [P = 0.001] and 131.79 ± 17.95 N/mm in the TTF + IS group [P < 0.001]). No significant difference in the mean stiffness was found between the IS group and the TTF + IS group (P = 0.127).Conclusions:In this biomechanical study, supplementary fixation with transtibial tubercle sutures increased the ultimate failure load during load-to-failure testing for PCL reconstruction.

简介：Objective:Laryngealreconstructionisneededtopreservelaryngealfunctioninpatientswhohaveundergoneextensiveverticalorfrontalpartiallaryngectomy.However,theprocedureremainsadifficultchallenge.Severalreconstructiontechniqueshavebeendescribed,butthesetechniquesposerisksofcomplicationssuchaslaryngealstenosis.Thisstudyaimedtoevaluatethepostoperativecourseandfunctionaloutcomesofanewtechniquethatcombinedamuscle-pediclehyoidboneandathyrohyoidflapduringlaryngealreconstructionaftertumorresection.Methods:Fourpatientsunderwentextensiveverticalpartialorfrontalpartiallaryngectomyforcancer.Aftertumorresection,laryngealreconstructionwasperformedusingtheproposedtechnique.Postoperativerecoverytime,complications,andoncologicresultswereevaluated.Results:Thefourpatientsweresuccessfullytreatedwiththeproposedtechnique.Nodyspnea,dysphagia,ordeathoccurredduringthepostoperativecourse.Decannulationwasperformedafteramedianof3days.Theaveragepostoperativehospitalstaywas7days.Short-termpostoperativefunctionalrecoverywasnormal.Nolaryngealstenosisortumorrecurrencewasobservedinanyofthefourpatientsafterafollow-upperiodofmorethan24months.Conclusion:Thecombinationofthemuscle-pediclehyoidboneandthethyrohyoidflapisareliableprocedureforlaryngealreconstructionafterextensiveverticalpartialorfrontalpartiallaryngectomy.

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简介：AbstractBackground:Computed tomography (CT) and magnetic resonance imaging (MRI) data can be fused to identify the tumor boundaries. This enables surgeons to set close but tumor-free surgical margins and excise the tumor more precisely. This study aimed to report our experience in performing computer navigation-aided joint-preserving resection and custom-made endoprosthesis reconstruction to treat bone sarcoma in the diaphysis and metaphysis of the femur and tibia.Methods:Between September 2008 and December 2015, 24 patients with bone sarcomas underwent surgical resection and joint-sparing reconstruction under image-guided computer navigation. The cohort comprised 16 males and eight females with a median age of 19.5 years (range: 12-48 years). The tumor location was the femoral diaphysis in three patients, distal femur in 19, and proximal tibia in two. The tumors were osteosarcoma (n = 15), chondrosarcoma (n = 3), Ewing sarcoma (n = 3), and other sarcomas (n = 3). We created a pre-operative plan for each patient using navigation system software and performed navigation-aided resection before reconstructing the defect with a custom-made prosthesis with extracortical plate fixation.Results:Pathological examination verified that all resected specimens had appropriate surgical margins. The median distance from the tumor resection margin to the joint was 30 mm (range: 13-80 mm). The median follow-up duration was 62.5 months (range: 24-134 months). Of the 24 patients, 21 remain disease free, one is alive with disease, and two died of the disease. One patient developed local recurrence. Complications requiring additional surgical procedures occurred in six patients, including one with wound hematoma, one with delayed wound healing, one with superficial infection, one with deep infection, and two with mechanical failure of the prosthesis. The mean Musculoskeletal Tumor Society score at the final follow-up was 91% (range: 80%-100%). The 5- and 10-year implant survival rates were 91.3% and 79.9%, respectively.Conclusions:Computer navigation-aided joint-preserving resection and custom-made endoprosthesis reconstruction with extracortical plate fixation is a reliable surgical treatment option for bone sarcoma in the diaphysis and metaphysis of the femur and tibia.

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简介：这张手稿用设计策略的织物集中于骨头修理/新生，并且加亮导致新奇nanocomposite系统的nanobiotechnology开发。大约650万破裂在美国每年发生，并且大约550,000个这些单个盒子要求了骨头接枝的申请。自然发生并且allogenous骨头最广泛地为骨头接枝被使用了基于的治疗；然而，象施主缺乏和感染的风险那样有重要问题。用合成、自然的biomaterials的选择被开发了，并且一些为要求骨头接枝的临床的应用程序是商业地可得到的。然而，设计很仔细模仿骨头织物在结构上，和罐头的理想的合成接枝仍然是大挑战在造骨细胞和祖先房间人口调制需要的功能。Nanobiomaterials，明确地，nanocompositeshydroxyapatite创作了(哈)或骨胶原极其正在答应接枝代用品。biocomposites能被制作模仿本国的骨头织物的材料作文，并且另外，当使用nano时--哈(减少的谷物尺寸)，一个人模仿本国的骨头的结构的安排。骨头生物学和结构的好理解对骨头mimicking接枝代用品的开发批评。哈并且能进一步调制regenerative/healing的优秀osteoconductive性质处理跟随破裂损害的骨胶原展览。与另外的聚合biomaterials结合将增强因此做新奇nano的机械性质--哈比得上人的骨头的基于的composites。我们用nanocomposites在最近的研究上报导为部分骨头缺点的新生作为粒子和nanofibers被制作了。在nanocomposites的研究，在理想的未来发展加亮一个枢轴的角色整形外科植入设备，然而进一步的重要前进是必要的完成临床的使用。

简介：Glassbasedbonecement(GBC)wassynthesizedbymixingCaO-SiO2-P2O5basedglasspowderwithammoniumphosphateliquidmedium.Bone-likehydroxyapatite(HAP,Ca10(PO4)6(OH)2)wasfoundtoformafterGBCwasimmersedinsimulatedbodyfluid(SBF).HAPcrystalgrewwithanincreasingtimealongcaxleandreachedabout200nminlengthafter30days,however,theendplanegranularityremained30～50nm.Thechemicalcomposition,crystalstructureandmorphologyofHAPformedfromGBCwereprovedtohavegreatresemblancewithlivingHAP.ItisbelievedthatGBCwasadesirablebiomedicalmaterialwithhighbioactivity.Furthermore,thehighcompressivestrengthguaranteedthepossibilityofGBCinclinicalapplication.

简介：Objective:Toinvestigateanewmethodtoconstructtissue-engineeringbonethatwillbeapplicableclinically.　　Methods:　Thecultured5thgenerationrabbitbonemarrowstromaosteoblasts(MSO)wasdissolvedin3%sodiumalginatesolution(thefinalconcentrationofsodiumalginateinthesolutionbeing1%,andMSO,5×106/L),andtheninoculatedintopreparedtrueboneceramic(TBC)andgelatinizedthebonebydribblingwithcalciumgluconate.Thestandardbonedefectmodelsweremadein48adultNewZealandrabbitsbothradius.Amongthe48rabbits,24wereinGroupsAandB,inwhichtheleftradiuswasimplantedwithgelatinizedMSO-TBC(GroupA)andrightradiusimplantedwithautograft-bone(GroupB);andtheother24wereincontrolgroupwhoseleftradiuswasimplantedwithnon-gelatinizedMSO-TBC(GroupC)andrightradiusimplantedwithgelatinizedTBC(GroupD).Outcomesoftheimplantedboneswereassessedbyradiology,pathologicalhistology,osteogeneticquantitativeanalysis,andbiomechanicsat2,4,8,12weekspostoperatively.Results:　InGroupsAandB,asatisfactorybonereparationandbonyunionwasnotedwithin12weeks.InGroupsCandD,bonereparationwasnotsatisfiedcomparedwithGroupAintermsofostogeneticquantityandbiomechanics.　Conclusions:　GelatinizedMSO-TBCisanidealartificialactivebonethatovercomesTBCshortcomingsoffragilenessandsmoothsurfacethatisnoteligibleforseedcellsadhesion.Itispromisingtoputintoclinicaluseextensively.

简介：Anovertphenotypeofaquaporin-1knockout(AQP1ko)miceisgrowthretardation,suggestingpossibledefectsinbonedevelopmentandmetabolism.Inthepresentstudy,weanalyzedthebonemineraldensity(BMD),bonecalciumandphosphoruscontents,andbonemetabolisminanAQP1komousemodel.TheBMDoffemursinAQP1komicewassignificantlylowerthanthatoflitter-matchedwildtypemiceasmeasuredbydualenergyX-rayabsorptiometry.Consistently,thecontentsofbonetotalcalciumandphosphoruswerealsosignificantlylowerinAQP1komice.ThereducedBMDcausedbyAQP1deficiencymainlyaffectmalemice.Bonemetabolicactivity,asindicatedby99mTc-MDPabsorptionmeasurements,wasremarkablyreducedinAQP1komice.TheseresultsprovidethefirstevidencethatAQP1playanimportantroleinbonestructureandmetabolism.

简介：ToobservepotentialeffectoftheengineeredbonemarrowstromalcelllineQXMSC1secretingIL-6(QXMSCIL-6)onacceleratingimmnunereconstitutioninsyngeneicbonemarrowtransplantationinmice,QXMSC1wastransfectedwiththeeukaryocyticexpressionvectorpcDNAIL-6,whichcontainedhIL-6cDNAbyliposome-mediatedgenetransfectingtechnique.G418-resistanceclonewasselectedbylimitingdilution.ThehighestsecretingclonewasselectedbyELISAassayandusedinanimalexperiments.Therecipientmice(BALB/c)werelethallyirradiatedandcotransplantedsyngeneicbonemarrow(10^7/mice)andtheQXMSCIIL-6(5×10^5/mice).LymphocyteproliferationinducedbyConAandLPS,helperTlymphocyteprecursor(HTLp),cytotoxicTlymphocyteprecursor(CTLp),plaque-formingcell(PFC),delayedtypehypersensitivity(DTH)wereexamined30,60daysinposttransplantationrespectively.TheresultsshowedthatlymphocytesproliferationtoConAandLPS,HTLp,CTLpincreased,DTHandPFCwereimprovedbycograftedstromalcellsQXMSCIIL-6on30,60daysafterBMT.TheseresultsdemonstratedthatthebonemarrowstromalcelllineQXMSC1IL-6transfectedwithIL-6(QXMSC11L-6)acceleratedimmnunereconstitutioninsyngeneicbonemarrowtransplantation.

简介：Paget'sdiseaseofboneisafocaldisorderofboneremodellingcausedbyabnormallyincreasedosteoclast-mediatedboneresorption.qtleaffectedboneschangeinshaw,sizeanddirection,whiletherestoftheskeletonremainsnormal.TheclinicalconsequencesofthediseasewerefirstdescribedbySirJanlesPagetin1876(Figure1).

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简介：你听说过GGBONE吗？就是那动画片中的猪猪侠，但是，你知道我的GGBONE吗，那就是我的QQ宠物猪猪。

简介：BackgroundTheVisibleHumanProject(VHP)initiatedbytheU.S.NationalLibraryofMedicinehasdrawnmuchattentionandinterestsfromaroundtheworld.TheVisibleChineseHuman(VCH)projecthasstartedinChina.ThecurrentstudyaimsatacquiringafeasiblevirtualmethodologyforreconstructingthetemporalboneoftheChinesepopulation,whichmayprovideanaccurate3-Dmodelofimportanttemporalbonestructuresthatcanbeusedinteachingandpatientcareformedicalscientistsandclinicians.MethodsAseriesofsectionalimagesofthetemporalboneweregeneratedfromsectionslicesofafemalecadaverhead.Oneachsectionalimage,SOIs(structuresofinterest)weresegmentedbycarefullydefiningtheircontoursandfillingtheirareaswithcertaingrayscalevalues.Theprocessedvolumedataweretheninductedintothe3DSlicersoftware(developedbytheSurgicalPlanningLabatBrighamandWomen'sHospitalandtheMITAILab)forresegmentationandgenerationofasetoftaggedimagesoftheSOIs.3DsurfacemodelsofSOIswerethenreconstructedfromtheseimages.ResultsThetemporalboneandstructuresinthetemporalbone,includingthetympaniccavity,mastoidcells,sigmoidsinusandinternalcarotidartery,weresuccessfullyreconstructed.Theorientationofandspatialrelationshipamongthesestructureswereeasilyvisualizedinthereconstructedsurfacemodels.ConclusionThe3DSlicersoftwarecanbeusedfor3-dimensionalvisualizationofanatomicstructuresinthetemporalbone,whichwillgreatlyfacilitatetheadvanceofknowledgeandtechniquescriticalforstudyingandtreatingdisordersinvolvingthetemporalbone.

简介：ObjectiveToinvestigatetheadvantagesofcanalwallreconstruction(CWR)mastoidectomy,asingle-stagetechniqueforcholesteatomaremovalandposteriorexternalcanalwallreconstruction,overtheopenandclosedproceduresintermsofcholesteatomarecurrence.Methods:BetweenJune2002andDecember2005,38patients(40ears)withcholesteatomawereadmitedtoSunYat-SenMemorialHospitalandreceivedsurgicaltreatments.Ofthesepatients,25weremalewithagesrangingbetween11and60years(mean=31.6years)and13werefemalewithagesrangingbetween20and65years(mean=38.8years).Canalwallreconstruction(CWR)mastoidectomywasperformedin31earsandcanalwalldown(CWD)mastoidectomyin9ears.Conchacartilagewasusedforearcanalwallreconstructionin22ofthe31CWRproceduresandcorticalmastoidbonewasusedintheremaining9cases.ResultsAt0.5to4yearsfollowup,allbutonepatientsremainedfreeofsignsofcholesteatomarecurrence,i.e.,noretractionpocketorcholesteatomamatrix.Onepatient,asmoker,neededrevisionsurgeryduetocholesteatomarecurrence1.5yearaftertheinitialoperation.Therecurrenceratewastherefore3.2%(1/31).CholesteatomarecurrencewasmonitoredusingpostoperativeCTscanswheneverpossible.Inthecasethatneededarevisionprocedure,aretractionpocketwasidentifiedbyotoendoscopyintheparsflacidaareathateventuallyevolvedintoacholesteatoma.Apocketextendingtotheepitympanumfilledwithcholesteatomamatrixwasconfirmedduringtherevisionoperation,Adecisiontoperformamodifiedmastoidectomywasmadeasthepatientrefusedtoquitsmoking.Themeanair-bonegapinpuretonethresholdwas45dBbeforesurgeryand25dBafter(p<0.05).Therewasnodifferencebetweenusingconchacartilageandcorticalmastoidboneforthereconstructionregardingair-bonegapimprovement,CTfindingsandotoendoscopicresults.ConclusionCWRmastoidectomycanbeusedformostpatientswithacquiredmiddleearcholesteatoma,inc

简介：AnalgorithmbasedoneigenanalysistechniqueandWalsh-Hadamardtransform(WHT)isproposed.Thealgorithmcontainstwosteps.Firstly,thereceivedsequenceisdividedintotemporalwindows,andacovariancematrixiscomputed.Thelinearfeedbackshiftregister(LFSR)sequenceisreconstructedfromthefirsteigenvectorofthismatrix.Secondly,equationsaccordingtotherecoveredLFSRsequenceareconstructed,andtheWalshspectrumcorrespondingtotheequationsiscomputed.ThefeedbackpolynomialofLFSRisestimatedfromtheWalshspectrum.Thevalidityofthealgorithmisverifiedbythesimulationresult.Finally,casestudiesarepresentedtoillustratetheperformanceoftheblindreconstructionmethod.