ADSORPTION OF PROTEIN ON NANOPARTICLES-中国期刊网

摘要 Theadsorptionofproteinonnanoparticleswasstudiedbyusingdynamiclightscatteringtomeasurethehydrodynamicsizeofbothpureproteinandnanoparticlesadsorbedwithdifferentamountsofprotein.Thethicknessoftheadsorbedproteinlayerincreasesasproteinconcentration,butdecreasesastheinitialsizeofnanoparticles.Afterproperlyscalingthethicknesswiththeinitialdiameter,weareabletofitallexperimentaldatawithasinglemastercurve.Ourexperimentalresultssuggestthattheadsorbedproteinsformamonolayeronthenanoparticlesurfaceandtheadsorbedproteinmoleculesareattachedtotheparticlesurfaceatmanypointsthroughapossiblehydrogen-bonding.Ourresultsalsoindicatethatasproteinconcentrationincreases,theoverallshapeoftheadsorbedproteinmoleculecontinuouslychangesfromaflatlayerontheparticlesurfacetoastretchedcoilextendedintowater.Duringthechange,thehydrodynamicvolumeoftheadsorbedproteinincreaseslinearlywithproteinconcentration.

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ADSORPTION OF PROTEIN ON NANOPARTICLES

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ADSORPTION OF PROTEIN ON NANOPARTICLES

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