简介：

简介：Inthepresentstudy,aseriesofnovelnitricoxide-hydrogensulfidereleasingderivativesof(S)-3-n-butylphthalide((S)-NBP)weredesigned,synthesized,andevaluatedaspotentialantiplateletagents.CompoundNOSH-NBP-5displayedthestrongestactivityininhibitingthearachidonicacid(AA)-andadenosinediphosphate(ADP)-inducedplateletaggregationinvitro,with3.8-and7.0-foldmoreeffectivenessthan(S)-NBP,respectively.Furthermore,NOSH-NBP-5couldreleasemoderatelevelsofNOandH2S,whichwouldbebeneficialinimprovingcardiovascularandcerebralcirculation.Moreover,NOSH-NBP-5couldrelease(S)-NBPwhenincubatedwithratbrainhomogenate.Inconclusion,thesefindingsmayprovidenewinsightsintothedevelopmentofnovelantiplateletagentsforthetreatmentofthrombosis-relatedischemicstroke.

简介：摘要目的RNA结合基序蛋白10(RBM10)突变体(N392S)的鉴定及探究其对肺腺癌细胞功能的影响。方法收集天津医科大学肿瘤医院生物样本库108例肺腺癌(LUAD)组织样本，进行RBM10外显子的sanger测序。将野生型RBM10及RBM10突变体(N392S)通过慢病毒感染的方法分别转入肺癌细胞，转入空载体的细胞作为对照。采用细胞增殖实验(MTT)、平板克隆实验、划痕实验和侵袭实验分析RBM10突变体对肺癌细胞增殖和迁移能力的影响，通过实时荧光定量聚合酶链反应(qPCR)检测含有和不含第9外显子的NUMB转录本核糖核酸(RNA)水平的变化分析RBM10突变体对NUMB第9外显子的剪切调控作用。组间数据比较采用t检验。结果在LUAD组织样本中检测到一种新的RBM10错义突变(N392S)；与空载体对照细胞比较，表达野生型RBM10的A549-RBM10-Wild和H358-RBM10-Wild细胞增殖明显变慢(1.28±0.01比1.43±0.03，t＝7.866，P<0.01；1.27±0.03比1.70±0.05，t＝11.840，P<0.01)，而表达突变体RBM10(N392S)的A549-RBM10-Mut和H358-RBM10-Mut细胞增殖能力高于表达野生型RBM10细胞系(1.49±0.13比1.28±0.01，t＝2.851，P<0.05；1.62±0.10比1.27±0.03，t＝5.823，P<0.01)；在平板克隆实验中，表达RBM10突变体的A549和H358细胞克隆形成率高于表达野生型RBM10的A549和H358细胞克隆形成率(53.00±3.61比28.60±4.34，t＝9.675，P<0.01；42.40±3.98比18.60±4.34，t＝9.047，P<0.01)；细胞划痕实验显示，野生型RBM10较对照组抑制肺腺癌细胞的迁移(56.28±13.49比100.00±10.27，t＝4.465，P<0.01)，而突变体较野生型则失去抑制细胞迁移的能力(138.94±20.18比56.28±13.49，t＝5.898，P<0.01)；Transwell实验证实，野生型RBM10较对照组抑制肺腺癌细胞的迁移(158.00±36.81比497.25±38.20，t＝15.093，P<0.01)，突变体较野生型RBM10失去抑制肺腺癌细胞迁移的能力(474.00±48.63比158.00±36.81，t＝12.424，P<0.01)；分析RBM10对NUMB第9外显子的剪切调控作用，随着野生型RBM10质粒浓度的递增，野生型RBM10促进NUMB第9外显子(NUMB-EXON 9)的跳读使得NUMB短剪接转录本水平的增加，而随着RBM10(N392S)质粒浓度的递增，NUMB短剪接转录本水平没有变化。结论RBM10突变体(N392S)丧失了野生型RBM10抑制LUAD细胞增殖与迁移的功能，并可能通过调控NUMB蛋白剪切影响LUAD细胞功能。

简介：摘要目的比较S-ReSC和S.T.O.N.E.评分系统对经皮肾镜取石术后结石清除率的预测价值。方法收集2016年9月至2019年4月在本院接受F24标准通道经皮肾镜取石术治疗的113例结石患者的临床资料，根据术后结石是否清除分为结石清除组82例和结石残留组31例。根据S-ReSC和S.T.O.N.E.评分系统分别对患者进行S-ReSC和S.T.O.N.E.评分，采用受试者工作特征(ROC)曲线分析S-ReSC和S.T.O.N.E.评分系统对结石清除的预测价值，采用多因素logistic回归模型分析影响结石清除的因素。结果结石清除组手术时间、术中出血量和住院时间均低于结石残留组(t＝2.325、2.726、2.301，P<0.05)；结石清除组S-ReSC评分和S.T.O.N.E.总评分低于结石残留组(t＝10.673、5.147，P<0.05)，两组S.T.O.N.E.评分中结石最大截面积、皮肾通道距离、肾积水程度、累及肾盏个数四项评分分布比较，差异有统计学意义(χ2＝9.296、4.543、4.292、8.533，P<0.05)，两组结石密度评分比较，差异无统计学意义(χ2＝2.105，P>0.05)；S-ReSC评分、S.T.O.N.E.总评分、结石最大截面积、肾积水程度、累及肾盏个数与结石清除状态有相关性(P<0.05)，而皮肾通道距离、结石密度与结石清除状态无相关性(P>0.05)；S-ReSC评分、S.T.O.N.E.评分预测结石清除的ROC曲线下面积(AUC)分别为0.875(95%CI：0.796～0.953)、0.751(95%CI：0.655～0.847)，S-ReSC评分预测结石清除的AUC大于S.T.O.N.E.评分(Z＝2.117，P＝0.034)；多因素logistic回归分析显示，手术时间、S-ReSC评分和S.T.O.N.E.评分是结石清除的影响因素。结论S-ReSC和S.T.O.N.E.评分系统均是预测经皮肾镜取石术后结石清除率的有效方法，S-ReSC评分的预测准确性更好。

简介：

简介：目的探讨特勤人员S_ⅠS_ⅡS_Ⅲ综合征特点。方法选取Ⅰ、Ⅱ、Ⅲ标准导联同时存在S波的患者心电图,分析Ⅰ、Ⅱ、Ⅲ、aVR、V1、V5导联R波与S波比值、心电轴及年龄、体型以及病史等相关参数。结果（1）S_ⅠS_ⅡS_Ⅲ综合征发生率低,仅0.9%（51/5639人）;（2）51例S_ⅠS_ⅡS_Ⅲ综合征患者心电轴偏移范围宽,在-110°～＋178°,平均（27.49±79.74）°,其中心电轴正常19例（37.3%）、右偏15例（29.4%）、左偏13例（25.5%）、心电轴不确定4例（7.8%）;（3）S_ⅠS_ⅡS_Ⅲ综合征多见于体质量指数偏低即呈瘦长体型年轻人,体质量指数〈24.0者占58.8%（30/51）;（4）疗养体检无其他异常发现。结论健康特勤人员可出现S_ⅠS_ⅡS_Ⅲ综合征,多见于瘦长体型年轻人,可能系正常心电图变异,但诊断要慎重,须经心脏超声及X射线等检查排除器质性疾病。

简介：希望大家能够抽出宝贵的时间，填写本刊中随附的《读者调查表》，并将之按照表中地址寄回我社，我们将从中抽取50名幸运读者赠送礼品（礼品详见读者俱乐部）。请大家准确填写您的联系方式和地址，以便我们为您发送精美礼品。美丽源自健康，感谢您关注《健康与美容》!

简介：目的:首先,鉴于目前用于预测经皮肾镜碎石术相关结果的评分系统使用繁琐、未被证实并且临床应用有限,本研究旨在提出一种新型的肾结石手术分级系统——S.T.O.N.E经皮肾镜碎石术评分系统,以使经皮肾镜碎石术的相关研究标准化。其次,应用本系统预测经皮肾镜碎石术的结石清除率和围手术期相关指标。方法:共有5个通过CT平扫获得的可重复参数被纳入本研究,分别是:结石大小(S)、穿刺通道长度(T)、梗阻情况(O)、受累肾盏数目(N)和结石

简介：在床上辗转反侧,却总是难以入睡?好不容易睡着了半夜总是频频醒来?怀孕之后,随着激素水平的变化,以及肚子越来越大,使得准妈妈多年来养成的睡眠姿势和习惯变得不再舒适。有好的睡眠才有健康。在怀孕初期,本来就容易有疲倦的问题,比孕前需要更多的睡眠与休息,许多医生会建议准妈妈每天晚上10点之前就寝,睡足8至9个小时。但是,许多准妈妈却仍然苦于无法安然入眠。其实,造成失眠的原因可能有很多,包括心理上对于怀孕的焦虑以及生理上的尿频、抽筋等,都是失眠可能的原因。

简介：当宝宝被娩出那一刻,新妈妈真是费尽了九牛二虎之力,身体筋疲力尽。产后好好地补一补,多吃一些高营养的食物就成为了惯例。要注意的是,进补是个技术活儿,别只顾埋头把自己吃成个大胖子。误区1产后马上大量喝汤当不少新妈妈给被推出产房的时候,家人早已提着保温桶备好了催乳汤。传统习俗认为,产后马上喝汤有利于身体恢复,也有利于通乳。但实际上,产后新妈妈喝汤急不得。尤其是剖宫产新妈妈,一定要要在身体排气之后才能喝汤,否则容易发生肠胀气。

简介：AIM:Toinvestigatetheeffectsofhydrogen-richsaline(HRS)onmicrogliaactivationandSirtuintype1(Sirt1)inratswithN-methyl-N-nitrosourea(MNU)-inducedretinitispigmentosa(RP).METHODS:Ratsweredividedintonorm(N)group,model(M)groupandHRS(H)group.RatsinMandHgroupsweregivensalineandHRSrespectivelypriortoandafteradministrationofMNU.Atoneday(d1)andd3afterwards,electroretinogramandhistologicalexaminationwereperformedtoconfirmtheeffectsofHRSonretinalfunctionandstructureofMNU-inducedRP.Immunofluorescencestainingofanti-ionizedcalcium-bindingadaptermolecule1(Iba1),amakerofmicrogliacells,wasperformed,withquantitativereal-timepolymerasechainreaction(qRT-PCR)foritsmRNAquantification.Moreover,Sirt1mRNAandproteinexpressionintheretinasweredetectedbyWesternblotandqRT-PCR.RESULTS:HRSpreservedtheretinalfunctionandmitigatedthereductionofphotoreceptordegenerationinMNU-treatedretinas.ThepresenceofmicrogliacellswassomewhatmoreobviousinHgroupthanthatinMgroupatd1.HRSsuppressedthefurtheractivationofmicrogliacells,withthenumberofmicrogliacellslessthanthatofMgroupatd3.ResultsofqRT-PCRofIba1wereconsistentwiththoseofimmunofluorescencestaining,withthemRNAexpressionofIba1inHgroupmoreintensivethanthatofMgroupatd1(P<0.05),whilelessthanthatofMgroupatd3(P<0.05).Furthermore,theSirt1mRNAandproteinexpressiondecreasedafterMNUadministration,whileHRSmitigatedtheMNU-induceddownregulationofSirt1.CONCLUSION:HRScaneffectivelykeepmicrogliaactivationinducedbyMNUtoanappropriateextent,whileupregulateSirt1inMNU-inducedRP.

简介：Thequestforneuroprotectivedrugstoslowtheprogressionofneurodegenerativediseases(NDDs),includingAlzheimer'sdisease(AD),Parkinson'sdisease(PD),andHuntington'sdisease(HD),hasbeenlargelyunrewarding.Preclinicalevidencesuggeststhatrepurposingquetiapine,lithium,valproate,fluoxetine,donepezil,andmemantineforearlyandpre-symptomaticdisease-modificationinNDDsmaybepromisingandcanspareregulatorybarriers.Theliteratureofthesepsychotropicsinearlystageandpre-symptomaticAD,PD,andHDisreviewedandpropitiousfindingsfollow.Mildcognitiveimpairment(MCI)phaseofAD:salutaryhumanrandomizedcontrolledtrialfindingsforlow-doselithiumand,inselectedpatients,donepezilawaitreplication.Pre-symptomaticAD:humanepidemiologicaldataindicatethatlithiumreducesADrisk.Animalmodelstudies(AMS)revealencouragingresultsforquetiapine,lithium,donepezil,andmemantine.EarlyPD:valproateAMSfindingsshowpromise.Pre-symptomaticPD:lithiumandvalproateAMSfindingsareencouraging.EarlyHD:uncontrolledclinicaldataindicatenon-progressionwithlithium,fluoxetine,donepezil,andmemantine.Pre-symptomaticHD:lithiumandvalproateareauspiciousinAMS.Manyotherpromisingfindingsawaitingreplication(valproateinMCI;lithium,valproate,fluoxetineinpre-symptomaticAD;lithiuminearlyPD;lithium,valproate,fluoxetineinpre-symptomaticPD;donepezilinearlyHD;lithium,fluoxetine,memantineinpre-symptomaticHD)arereviewed.Dose-andstage-dependenteffectsareconsidered.Suggestionsforsignal-enhancementinhumantrialsareprovidedforeachNDDstage.

简介：

简介：是不是觉得老在减肥,腿却一直没有瘦下来?只想瘦腿却先减了胸围?茨慕别人笔直修长的美腿!看归看,想归想,关键还是要行动起来,只要你能坚持,你也能拥有一双性感修长的"纤"腿!

简介：花了大把金钱购买了各种健康卡、游泳卡，一阵新鲜劲过后就都扔到一旁了,这样的你有木有？下决心每天做面部按摩、每天做仰卧起坐，但总会因各种缘由最后还是放弃了＇这样的你有木有？本期我们的总力特辑《按摩＆瑜伽零压力方程式》就是为这样的你量身定制的专属健康内容!

简介：ＱＵＡＬＩＴＡＴＩＶＥＳＴＵＤＹＯＦＳＩＡＬＯＭＵＣＩＮＳＣＨＡＮＧＥＳＤＵＲＩＮＧＮ－ＭＥＴＨＹＬ－Ｎ－ＮＩＴＲＯＳＯＵＲＥＡ－ＩＮＤＵＣＥＤＣＯＬＯＮＩＣＣＡＲＣＩＮＯＧＥＮＥＳＩＳＩＮＭＩＣＥＷａｎｇＱｉａｎｇ王强；ＷａｎｇＹｕａｎｈｅ王元和；...

简介：本文研究了地表水源水一年中NH3－N，NO2－N含量与季节变化的关系，总结出一定的规律，从而对水质处理提供可靠的数据。

简介：Neurotrophicfactorscompriseessentialsecretedproteinsthathaveseveralfunctionsinneuralandnon-neuraltissues,mediatingthedevelopment,survivalandmaintenanceofperipheralandcentralnervoussystem.Therefore,neurotrophicfactorissuehasbeenextensivelyinvestigatedintothecontextofneurodegenerativediseases.Alzheimer'sdiseaseandParkinson'sdiseaseshowchangesintheregulationofspecificneurotrophicfactorsandtheirreceptors,whichappeartobecriticalforneuronaldegeneration.Indeed,neurotrophicfactorspreventcelldeathindegenerativeprocessesandcanenhancethegrowthandfunctionofaffectedneuronsinthesedisorders.Basedonrecentreports,thisreviewdiscussesthemainfindingsrelatedtotheneurotrophicfactorsupport–mainlybrain-derivedneurotrophicfactorandglialcellline-derivedneurotrophicfactor–inthesurvival,proliferationandmaturationofaffectedneuronsinAlzheimer'sdiseaseandParkinson'sdiseaseaswellastheirputativeapplicationasnewtherapeuticapproachforthesediseasesmanagement.

简介：AbstractObjective:The prevalence of midline birth defects, such as gastroschisis, has increased worldwide, over the last few decades. This study aims to explore the prevalence, maternal epidemiological characteristics, and natural history of neonates affected by gastroschisis at the University Hospital of León city, Nicaragua.Methods:Data were collected from the birth defect surveillance system of the Hospital Oscar Danilo Rosales (HEODRA). The analysis included all pregnancies that had gastroschisis complications between January 1 and December 31, 2020. The prevalence of gastroschisis was calculated according to maternal age. The mothers were interviewed, and the clinical records of the newborns were reviewed.Results:Among the 4,460 deliveries included in this study, four cases of gastroschisis were identified, including three live births and one stillbirth. The gastroschisis rate was 8.9 per 10,000 live births (95% confidence interval [CI]: 0.18-17.8). The prevalence among mothers younger than 20 years and those older than 20 years was 26.4 (95% CI: -3.43 to 56.25) and 3.01 (95% CI: 2.89-8.90)/10,000 births, respectively. Mothers of gastroschisis-affected fetuses were of rural origin (n = 3), had normal body mass indexes (n = 3), were exposed to tobacco and wood smoke (n = 2), and one was exposed to pesticides during the periconceptional period. Primary closure of the gastroschisis was performed on one patient, and complex gastroschisis for intestinal perforation was observed in another patient. The mean hospitalization duration was 33 days, and two patients were discharged alive.Conclusions:Gastroschisis was a significant birth defect among children delivered at HEODRA in 2020. Its prevalence in Nicaragua was higher than that in other countries in the region. All complicated pregnancies were young women with unplanned pregnancies, from rural areas, with exposure to secondhand smoke, and without vitamin supplements before or during the first trimester of pregnancy. Only 67% of infants survived after hospital discharge.

简介：2013年全国两会期间，政协委员们谈论最多的话题之一便是北京的雾霾。全国政协委员、京剧表演艺术家、梅兰芳之子梅葆玖表示，因为北京的雾霾天气，他的很多演员同事演唱都有了问题，嗓子不舒服，有痰，甚至声音都发生了变化。究竟什么是嗓音疾病呢？