简介：AbstractPurpose:The incidence of acute lung injury (ALI) in severe trauma patients is 48% and the mortality rate following acute respiratory distress syndrome evolved from ALI is up to 68.5%. Alveolar epithelial type 1 cells (AEC1s) and type 2 cells (AEC2s) are the key cells in the repair of injured lungs as well as fetal lung development. Therefore, the purification and culture of AEC1s and AEC2s play an important role in the research of repair and regeneration of lung tissue.Methods:Sprague-Dawley rats (3-4 weeks, 120-150 g) were purchased for experiment. Dispase and DNase I were jointly used to digest lung tissue to obtain a single-cell suspension of whole lung cells, and then magnetic bead cell sorting was performed to isolate T1α positive cells as AEC1s from the single-cell suspension by using polyclonal rabbit anti-T1a (a specific AEC1s membrane protein) antibodies combined with anti-rabbit IgG microbeads. Afterwards, alveolar epithelial cell membrane marker protein EpCAM was designed as a key label to sort AEC2s from the remaining T1α-neg cells by another positive immunomagnetic selection using monoclonal mouse anti-EpCAM antibodies and anti-mouse IgG microbeads. Cell purity was identified by immunofluorescence staining and flow cytometry.Results:The purity of AEC1s and AEC2s was 88.3% ± 3.8% and 92.6% ± 2.7%, respectively. The cell growth was observed as follows: AEC1s stretched within the 12-16 h, but the cells proliferated slowly; while AEC2s began to stretch after 24 h and proliferated rapidly from the 2nd day and began to differentiate after 3 days.Conclusion:AEC1s and AEC2s sorted by this method have high purity and good viability. Therefore, our method provides a new approach for the isolation and culture of AEC1s and AEC2s as well as a new strategy for the research of lung repair and regeneration.

简介：XPS(X光检查光电子光谱学)分光计EscA-750被用来分析激活的碳，磁性的试剂和磁性的激活的碳的表面特性。他们的C原子价的分发和变化是坚定的。结论是磁性的试剂能化学上在激活的碳的表面上被吸附。在微观结构的磁性的试剂的吸附的机制的解释被讨论。

简介：Wedescribeanoveltechnique,lowsurfaceenergyGasExpansionMolding(GEM),tofabricatemicrobubblearraysinpolydimethylsiloxane(PDMS)whichareincorporatedintoparallelplateflowchambersandtestedincellsortingandmicrocellcultureapplications.Thisarchitectureconfersseveraloperationaladvantagesthatdistinguishthistechnologyapproachfromcurrentlyusedmethods.HereinwedescribetheGEMprocessandtheparametersthatareusedtocontrolmicrobubbleformationandaVacuum-AssistedCoating(VAC)processdevelopedtoselectivelyandspatiallyalterthePDMSsurfacechemistryinthewellsandonthemicrochannelsurface.WedescriberesultsfrommicroflowimagevisualizationstudiesconductedtoinvestigatefluidstreamsaboveandwithinmicrobubblewellsandconcludewithadiscussionofcellculturestudiesinPDMS.

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简介：BACKGROUND:Microgliaareverysensitivetoenvironmentalchanges,oftenbecomingactivatedbypathologicalconditions.Activatedmicrogliacanexertadualroleininjuryandrepairinvariousdiseasesofthecentralnervoussystem,includingcerebralischemia,Parkinson’sdisease,andAlzheimer’sdisease.OBJECTIVE:Animmortalmicroglialcellline,BV2,wastreatedwithvaryingconcentrationsoflipopolysaccharide(LPS)toinduceapathologicalsituation.Supernatantwasharvestedandincubatedwithbonemarrowmesenchymalstemcellsand,concomitantly,bonemarrowmesenchymalstemcelldifferentiationwasobserved.DESIGN:Acontrolledobservation,invitroexperiment.SETTING:DepartmentofNeurology,FirstAffiliatedHospitalofChinaMedicalUniversity.MATERIALS:Fivemale2–3-week-oldSpragueDawleyratswerepurchasedfromAnimalLaboratoryCenterofChinaMedicalUniversityandincludedinthisstudy.Theprotocolwasperformedinaccordancewithethicalguidelinesfortheuseandcareofanimals.ThemicroglialcelllineBV2wasproducedbyCellResearchInstituteofChineseAcademyofSciences.LPSwasproducedbySigmaCompany,USA.METHODS:ThisstudywasperformedintheCentralLaboratoryofChinaMedicalUniversityfromSeptember2006toMarch2007.Ratfemoralandtibialbonemarrowwascollectedforseparationandprimarycultureofbonemarrowmesenchymalstemcells.Bonemarrowmesenchymalstemcellculturesweredividedinto5groups:controlgroup,non-activatedgroup,aswellaslow-,medium-,andhigh-doseLPSgroups.Inthecontrolgroup,bonemarrowmesenchymalstemcellswereculturedwithDulbecco’smodifiedEagle’smedium(DMEM)supplementedwithfetalbovineserum(volumefraction0.1).Inthenon-activatedgroup,bonemarrowmesenchymalstemcellswereincubatedwithnon-activatedBV2supernatant.Inthelow-,medium-,andhigh-doseLPSgroups,bonemarrowmesenchymalstemcellswereincubatedwithLPS(0.01,0.1and1μg/L,respectively)-activatedBV2supernatant.MAIN

简介：Classicalsortingbyreversalsusestheunit-costmodel,thatis,eachreversalconsumesanequalcost.Thismodellimitsthebiologicalmeaningofsortingbyreversal.Benderandhiscolleaguesextendeditbyassigningacostfunctionf(l)=lαforallα≥0,wherelisthelengthofthereversedsubsequence.Inthispaper,weextendtheirresultsbyconsideringamodelinwhichlongreversalsareprohibited.Usingthesamecostfunctionaboveforpermittedreversals,wepresenttightornearlytightboundsfortheworst-casecostofsortingbyreversals.Thenwedevelopalgorithmstoapproximatetheoptimalcosttosortagiven0/1sequenceaswellasagivenpermutation.OurproposedproblemsaremorebiologicallymeaningfulandmorealgorithmicallygeneralandchallengingthantheproblemconsideredbyBenderetal.Furthermore,ourboundsaretightandnearlytight,whereasouralgorithmsprovidegoodapproximationratioscomparedtotheoptimalcosttosort0/1sequencesorpermutationsbyreversals.

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简介：Themainfunctionofelectronicsupportmeasuresystemistodetectthreatingsignalsinordertotakecountermeasuresagainstthem.Toaccomplishthisobjective,aprocessofassociatingeachinterleavedpulsewithitsemittermustbedone.Thisprocessistermedsortingorde-interleaving.Anovelpointsymmetrybasedradarsorting(PSBRS)algorithmisaddressed.Inordertodealwithallkindsofradarsignals,thesymmetrymeasuredistanceisusedtoclusterpulsesinsteadoftheconventionalEuclideandistance.Thereferencepointsofthesymmetricalclustersareinitializedbythealternativefuzzyc-means(AFCM)algorithmtoamelioratetheeffectsofnoiseandthefalsesorting.Besides,thedensityfiltering(DF)algorithmisproposedtodiscardthenoisepulsesorclutter.Theperformanceofthealgorithmisevaluatedundertheeffectsofnoiseandmissingpulses.IthasbeenobservedthatthePSBRSalgorithmcancopewithalargenumberofnoisepulsesanditiscompletelyindependentofmissingpulses.Finally,PSBRSiscomparedwithsomebenchmarkalgorithms,andthesimulationresultsrevealthefeasibilityandefficiencyofthealgorithm.

简介：Inthispaper,sixkindsofactivatedcarbonssuchasAg^+-activatedcarbon,Cu^2+activatedcarbon,Fe^3+-activatedcarbon,activatedcarbon,Ba^2+-activatedcarbonandCa^2+activatedcarbonwereprepared.Themodelforestimatingactivatedenergyofdesorptionwasestablished.Temperature-programmeddesorption(TPD)experimentswereconductedtomeasuretheTPDcurvesofn-hexanolandthenestimatetheactivationenergyfordesorptionofn-hexanolontheactivatedcarbons.Resultsshowedthattheactivationenergyforthedesorptionofn-hexanolontheAg^+-activatedcarbon,theCu^2+-activatedcarbonandtheFe^3+-activatedcarbonwerehigherthanthoseofn-hexanolontheactivatedcarbon,theCa^2+-activatedcarbonandtheBa^2+-activatedcarbon.

简介：Sarcoidosisisarareconditionofgranulomatousinfiltrationofmanytissuesofthebody,includingtheheart.Cardiacsarcoidosishasbeenchallengingtostudy,asitisoftenasymptomatic,althoughtheinitialpresentationcanbesuddencardiacdeath.Theincidenceandprevalencerateshavebeendifficulttoestablishandnoexpertagreeduponguidelinesfordiagnosisandmanagementofcardiacsarcoidosisexist,andclinicalmanifestationsarevaried.Thepathophysiologyofgranulomaformationinthemyocardiumaswellasothertissuesisgovernedbyimmuneresponsetosomeenvironmentalantigen.Geneticsisalsothoughttoplayarole,althoughgenealterationshavenotbeenextensivelystudied,andnospecificsetofgeneticmutationshasbeenidentifiedtoaidinidentificationofindividualsatriskofdevelopingdisease.Epigeneticfactorslikelyplayasignificantroleinmodulationofgeneexpressionwithrespecttoimmuneresponse.Thereisnostandardizedscreeningtoolfortheidentificationofcardiacsarcoidosis.Thepresenceofsystemicsarcoidosisandnew-onsetthird-degreeheartblockorventriculararrhythmiaswarrantsfurtherinvestigationforcardiacsarcoidosis.MRIandPETareusefulinhelpingtoidentifycardiacsarcoidosisbutarenotstand-alonetests.Endomyocardialbiopsyisthegoldstandardbuthasalowyieldowingtothepatchynatureofgranulomaformationinthemyocardium.Therapyshouldbeinstitutedearlyandinvolvesimmunosuppressivetherapywithpredominantuseofcorticosteroids.Arrhythmias,eitherventricularorhigh-gradeheartblocks,aremanagedwithdevicetherapy.Clinicalpresentationmaywarrantuseofantiarrhythmicagentsand/orcatheterablation.Survivalanddiseaseprognosisaredependentonearlydiagnosisandtreatment.Thisreviewdetailsthecurrentunderstandingofcardiacsarcoidosisandhighlightsdiagnosticstrategiesandtreatmentwiththeaimofguidingthecliniciantoearlyidentificationofpatientsandimplementationofappropriatemanagementinthisraredi

简介：Activatedcarbonfiberisonekindofimportantadsorptionmaterials.Thesenovelfibrousadsorbentshavehighspecificsurfaceareasorabundantfunctionalgroups,whichmakethemhavegreateradsorption/desorptionratesandlargeradsorptioncapacitiesthanotheradsorbents.Theycanbepreparedasbundle,paper,clothandfelttomeetvarioustechnicalrequirement.Theyalsoshowreductionproperty.Inthispaperthelatestprogressonthestudiesofthepreparationandadsorptionpropertiesofactivatedcarbonfibersisreviewed.Theapplicationofthesematerialsindrinkingwaterpurification,environmentalcontrol,resourcerecovery,chemicalindustry,andinmedicineandhealthcareisalsopresented.

简介：ThedynamiccompetititveadsorptionbehaviorsofdifferentbinaryorganicvapormixturesonACF-Psunderdifferentoperationconditonswereinvestigatedbygaschromatographyinthispaper,Thestudiedmixtruesincludedbenzene/toluene,toluene/xylene,benzene/isopropylbenzeneethlyacetate/tolueneandbenzene/ethylacetate.ExperimentalresultsshowthatvariousACF-Ps,aswithACF-W,canremovebothvaporsinbinaryvapormixtureswithover99%ofremovalefficiencybeforethebreakthroughpointofthemoreweaklyadsorbedvapor,Indynamiccompetitiveadsorption,themoreweaklyweakleadsorbedvapornoeonlypenetratesearly,butalsowillbedisplacedanddesorbedconsequentlybystrongeradsorbateandthereforeproducesarollingupinthebreakthroughcurve,TheACF-Pspreparedatdifferenttemperatuershavesomewhatdifferentadsorptionselectivity,Thefeedconcentrationratioofvapros,thelength/diameterratioandthethickofbedhaveeffectoncompetitiveadsorption.Thecompetitiveadsorptionabilityofavaporismainlyrelatedtoitsboilingpoint.Usually,thehighertheboilingpoint,thestrongerthevaporadsorbedonACF-P.

简介：StructuresofaseriesofactivatedcarbonfibersweremodifiedbyimpregnatingthemwithorganicandinorganicmaterialssuchasMethyleneblue(Mb)、p-nitrophenol(PNP)、NaClorbyoxidizingwithKMnO4orHNO3.Theinfluenceofporefillingorchemicaltreatmentontheirxenonadsorptionpropertieswasstudied.TheexperimentalresultsshowthatMbandPNPfillingofactivatedcarbonfibersresultinthedecreaseofxenonadsorptioncapacitiesofthesetreatedACFs,whichisduetothedecreaseoftheirsurfaceareaandmicro-porevolume.However,theadsorptioncapacityincreasesgreatlywithoxidizingtreatmentofactivatedcarbonfibersby7mol/LHNO3.

简介：磁性的珍珠的使液化行为上的外部磁场的影响的试验性的研究被执行。磁性的珍珠是主要由从研磨的煤燃烧从大量的发电厂苍蝇灰被恢复的Fe2O3组成的氧化铁的一张磁性的表格。由于它的丰富，低价格和物理的详细规格和化学性质，磁性的珍珠能被用作重媒介因为矿物质或稳固的废物基于密度差别弄干分离。这份报纸介绍磁性的珍珠的性质并且把磁性的珍珠fluidised病床操作的表演与或没有一个外部磁场作比较。试验性的结果证明一个外部磁场显著地改进象一致性和稳定性那样的磁性的珍珠的使液化表演。

简介：ＡＮＥＷＰＬＡＮＡＲＢＯＯＬＥＡＮＯＰＥＲＡＴＩＯＮＡＬＧＯＲＩＴＨＭＢＡＳＥＤＯＮＬＩＮＥＳＥＧＭＥＮＴＳＯＲＴＩＮＧＭａＬｉｎ；ＤｕＸｉｑｉｎｇ；ＧｕａｎＲｏｎｇｃｈａｎｇＳＭＯＯＴＨＳＵＲＦＡＣＥＩＮＴＥＲＰＯＬＡＴＩＯＮＯＶＥＲＡＲＢＩＴＲＡ...

简介：Theworldhasbecomehighlydependentonreliablerefrigerationtechnology.Withoutit,ourfoodsupplywouldbestillseasonalandlimitedtolocallyproducednonperishableitems;comfortablelivingconditionsviaclimatecontrolwouldbeimpossible;andcertainmedicaladvancements,e.g.MRIdiagnostics,organtransplantation,organandtissuecryo-storage,andcryo-surgerywouldbeimpossible.

简介：Physicalandchemicalpropertiesofthemagneticnanoparticlescoatedwitholeatehavebeeninvestigatedbymeansoftransmissionelectronmicroscopy(TEM),thermogravimetricanalysis(TGA),Mossbauerspectroscopy,superconductingquantuminterferencedevice(SQUID)magnetometry,infraredspectra(IR)andthecontactangledevice.TheresultsshowthatdopedAlionsinFe3O4nanoparticlesarelocatedontheoctahedralsitesofthespinelstructure.Oleateiscoatedonthemagneticnanoparticleswithtwolayersbychemicalabsorbing,theouterlayercanbewashedaway.Thewettingofthesurfaceofmagneticnanoparticlescoatedwithmonolayerhasbeenchangedfromhydrophilicitytohydrophobicity,andthenanoparticlescanbedispersedverywellinsomeorganicsolutions.

简介：增加的证据证明磁场和磁性的应答的脚手架能在支持骨头修理和新生起唯一的作用。这篇文章在situ在骨头新生上响应外部磁场探讨磁性的脚手架的synergistic效果。另外，使用在骨头的工具植入固定，本地药交货和干细胞区别的mimicking微型环境的磁性的脚手架的探索被介绍。我们也在骨头修理和新生讨论了磁性的应答的脚手架的可能的内在的机制和观点。

简介：Theadsorptionbehaviorofdyesonavarietyofsisalbasedactivatedcarbonfibers(SACF)hasbeenstudiedinthispaper.TheresultsshowthatthiskindofACFhasexcellentadsorptioncapacitiesforsomeorganic(dye)molecules.SACFcanremovenearlyallmethyleneblue,crystalviolet,bromophenolblueandEriochromeblueblackRfromwaterafterstaticadsorptionfor24h.at30℃.Theadsorptionamountscanreachmorethan400mg/gwhenadding50mgSACFinto50mldyesolution.Underthesameconditions,theadsorptionamountsofxylenolorangefluoresceinandEriochromeblackTwreelower.Ontheotherhand,theadsorptionamountschangealongwiththecharacteristicsofadsorbents.TheSACFsactivatedabove840℃,whichhavehigherspecificsurfaceareasandwiderporeradii,havehigheradsorptionamountsforthedyes.TheresearchingresultsalsoshowthattheadsorptionratesofdyesontoSACFsdecreasebytheorderofmethyleneblue,EriochromeblueblackRandcrystalviolet.