简介:Microbialcommunityfunctionaldiversityisasensitiveindicatorofsoil.Forestfirescanchangemicrobialcommunityfunctionaldiversity.Inthisresearch,thefiredsoilsampleswerecollectedfromHuzhongofDaxing’anlinginHeilongjiangProvince.ThefunctionaldiversityofsoilmicrobialcommunitywasdetectedbyBIOLOGsystem.Theaveragewellcolordevelopment(AWCD)inBIOLOGplatesindicatedtheabilityofcarbonsubstrateutilizationofmicrobialcommunity.TheindicesofShannon,SimpsonandMcintoshwerecalculatedtoshowtherichness,dominanceandevennessofthefunctionaldiversity,andtheprincipalcomponentanalysisofsubstratereactionsreflectedthemaincarbonsourcesutilizedbymicrobialcommunity.TheresultsshowedthatallthesamplesexhibitedthereductionofAWCDduringthefirst144hoursofincubation,buttherewereobviousdifferencesinthereductiondegreeamongthesamples.Allkindsoffireintensitiescouldinfluencemicrobialcommunityfunctionaldiversity.Thelowfireintensityincreasedtherichness(Shannon),dominance(Simpson)andevenness(Mcintosh)ofmicrobialcommunity.However,theintermediateandhighfireintensityreducedtheseparameters,suggestingthattheburnedsoilemittedgaseousnitrogen,anumberoforganiccarbonandavailablephosphorus,whichcausedthereductionofthemicroorganisminquantityandtype,andchangeinsoilingredients.Theorganicmaterialreducedintheburnedsoil,inthesametime,thepHvaluesrose,whichchangedenvironmentinwhichmicroorganismlives.Thechangedenvironmentmightnotbeconducivetoactivitiesofthemicroorganism.Theprincipalcomponentanalysisshowedthatthemaincarbonsourcesforsoilmicrobeswerecarbohydratesandaminoacidsandthecarbonsubstrateutilizationpatternsindifferentsamplesweresignificantdifferent.Theresultsindicatedthatfunctionaldiversityofsoilmicrobeswerealteredbyforestfires,andtheabilityofmicrobestoutilizecarbonsourceandthetypeofcarbonsourceswereaffectedbydif