简介:NuclearfactorkappaB(NF-κB)isoneofthebest-characterizedtranscriptionfactorsplayingimportantrolesinmanycellularresponsestoalargevarietyofstimuli,includinginflammatorycytokines,phorbolesters,growthfactors,andbacterialandviralproducts.TheaimofthisstudyistodemonstrateNF-κBexpressioninthemousecochleaanditsenhancementinresponsetolipopolysaccharides(LPS)andkanamycin(KA)treatment.MethodsKAtreatmentconsistedofsubcutaneousKAinjectionsat700mg/kgtwiceadaywithaneight-hourintervalbetweenthetwoinjectionsfor3or7days.ForanimalsintheLPStreatmentgroup,asingledoseof0.3mgLPSdissolvedin0.2mlsterilesalinewereinjectedintobothbullaethroughthetympanicmembraneandkepttherefor3hours.Animalsinthecontrolgroupreceivedsubcutaneoussalineinjectionfor7days.Followingimmmunohistochemichalprocessingwithrabbitpolyclonalanti-NF-κBp65antibodies,cryosectionsofthecochleawereexaminedforexpressionofNF-κBp65invariousstructuresinthecochlea.ResultsNF-κBp65expression,identifiedbypresenceofbrownreactionproductscharacteristicofDABimmunohistochemistry,wasvisibleinthespiralligament,spiralprominence,tectorialmembrane(TM),spiralganglionandnervefibers.RelativelyweakNF-κBp65expressionwasalsovisualizedintheorganofCorti.WithintheorganofCorti,theinnerhaircells(IHC),outerhaircells(OHC),innerpillarcells(IP),outerpillarcells(OP),Deiter'scells(DC),andBoettcher'scellsexhibitedstrongerstainingthantheinnersulcuscells,Hensen'scells(HC)andClaudius'cells.NoNF-κBp65expressionwasseeninthenucleusoftheIHCandOHC.NF-κBp65expressionwasincreasedinanimalsexposedtoLPSorKA,demonstratingsignificantdifferencesinthestainingbetweencontrolanimalsandLPS/KA-treatedanimals.NF-κBp65expressionwasnotsignificantlydifferentbetweenLPStreatedandKAtreatedanimalsorbetween3and7daysinKA-treatedanimals.Conclusio
简介:摘要目的:研究近视患者双眼暗视至明视状态下Kappa角水平偏移量及垂直偏移量的变化规律。方法:横断面研究。选取2020年11月至2021年12月在宁夏医科大学总医院门诊近视中心明确诊断为近视的患者,并采用单纯随机抽样的方法抽取120例(240眼),采用博士伦Orbscan II角膜地形图仪分别于暗视及明视条件下进行检查,记录明暗视下瞳孔直径大小、Kappa角的大小及水平偏移量和垂直偏移量。采用配对t检验比较左右眼在暗视和明视下的瞳孔直径和Kappa角大小;采用散点分布图分析双眼在明暗视下Kappa角的象限分布。结果:左右眼在暗视状态下的瞳孔直径均明显大于明视状态下的瞳孔直径,差异有统计学意义(t=13.67,P<0.001;t=13.48,P<0.001)。暗视与明视状态下左眼的Kappa角均大于右眼的Kappa角,差异有统计学意义(t=4.15,P=0.021;t=5.27,P=0.008)。由暗视转向明视状态时,双眼Kappa角的分布呈现镜像对称,水平方向主要向鼻侧位移,垂直方向主要向上方位移。右眼矢量位移(0.168±0.100)mm,左眼矢量位移(0.171±0.069)mm;87.5%右眼矢量位移小于0.3 mm,92.5%左眼矢量位移小于0.3 mm,绝大多数近视患者的矢量位移在0.3 mm以内。结论:从暗视至明视状态下,随着瞳孔缩小,近视患者的Kappa角分布主要向鼻上方位移。