简介：Diversityofbacteriawasstudiedindeep-seasedimentsfromtheShikokuBasinintheNorthwestPacificOceanbyPCR,RFLPandsequenceanalysisof16SrDNAandcomparingwithGenbankdatabase.BasedontheRFLPprofilegenerated,77clonesfromthe16SrDNAlibraryweredividedinto27types.Phylogeneticanalysisshowedthatthe27independentclonesfellintofourgroups:Proteobacteria(62.96%),Chloroflexi(14.81%),Planctomycetes(14.81%)andAcidobacteria(7.41%).Amongallsequencedclones,6wererelatedtothesulfurorsulfatemetabolismbacteriaandtheresultsalsodemonstratedthatsomebacteriaindeep-seasedimentshadrelationtomatter-energycirculation.

简介：能够lysing的所有以前报导的细菌的种类有害水藻从有害水藻花蕾在发生了的沿海的环境被孤立。由于在一个海藻的花蕾的水藻细胞溶解细菌的低集中，由存在方法孤立水藻细胞溶解细菌是困难的。在这篇论文，二水藻细胞溶解细菌的紧张，P01；P03，从在在移开水藻是高度有效的海绵上使不能调动的一个简历系统被孤立；microcystins。他们的lysing模式；Microcystisaeruginosa上的效果被学习了。降级为M这二紧张处理的结果表演。当时，与一阶的反应给予的aeruginosa当模特儿集中在从0～1000μgL的范围的叶绿素[1]。降级率常数是0.1067，0.1274；0.2792为P01；0.0683，0.0744；0.02897为P03当细菌的密度是8.6×1时0[5]，8.6×10[6]；8.6×10[7]房间mL[1]分别地。而且，二细菌的紧张在M上有赞成lysin的效果不仅。aeruginosa，而且上小球藻；Scene-desmus。他们M上的lysin的效果。aeruginosa没要求物理房间到房间接触，但是由一个细胞外的产品的生产继续。细菌的紧张被16SrRNA基因的PCR扩大作为杆菌种类识别，强风分析，；有在GenBank核苷酸数据库的序列的比较。

简介：Thisstudyinvestigatedthebacterialdiversityofgutcontentofseacucumber(Apostichopusjaponicus)anditshabitatsurfacesedimentinabottomenhancementareausingPCR-baseddenaturinggradientgelelectrophoresis(DGGE)technique.BacterialdiversityevaluationshowedthatthevalueoftheShannon-WienerindexofgutcontentindifferentintestinalsegmentsofA.japonicusvariedbetween2.88and3.00,lowerthanthatofthesurroundingsediment(3.23).PhylogeneticanalysisshowedthatbacterialphylotypesingutcontentandthesurroundingsedimentofA.japonicuswerecloselyrelatedtoProteobacteriaincludingγ-,α-,δ-andε-proteobacteria,Bacteroidetes,Firmicute,andActinobacteria,ofwhichγ-proteobacteriawerepredominant.TheseresultssuggestedthattheseacucumberA.japonicuswascapableoffeedingselectively,andPCR-DGGEwasapplicableforcharacterizingthebacterialcommunitycompositioningutcontentandthesurroundingsedimentofseacucumber.Furtherinvestigationtargetinglonger16SrDNAgenefragmentsand/orfunctionalgeneswasrecommendedforobtainingmoreinformationofthediversityandfunctionofbacterialcommunityinthegutcontentofseacucumber.