简介：双向聚丙烯酰胺凝胶电泳按等电点和分子量对蛋白质进行分离，具有很高的分辨率，已经成为蛋白质组研究中的核心技术之一。目前的方法还难以满足真核生物蛋白质组研究的要求。自1975年O'Farrell提出该方法以来，针对应用中常见的一些问题，如样品的增溶及加载，样品的预分级分离，阴极漂移和凝胶上蛋白质的回收等，对这种技术进行了不断的改进。

简介：目的:目前中药天龙以假充真现象较多.为了探求更便捷、精准的壁虎及其伪品蜥蜴的鉴定方法,探讨其古方中分部位用药的相关实验依据进行该试验.方法:利用十二烷基硫酸钠聚丙烯酰胺凝胶电泳技术(SDS-PAGE),观察天龙及其伪品蜥蜴的电泳迁移率,并且对比其躯干、头、足及尾部等部位蛋白电泳区带的差异.结果:经实验鉴别,天龙与蜥蜴的电泳图谱差异显著,各有其特有谱带;且同一样品的不同部位之间谱带亦有区别.结论:SDS-PAGE图谱可以作为天龙及其伪品蜥蝎的有效鉴别依据,在古方中将动物分成各部位来用药的机理有进一步探究的必要.

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简介：Inthispaper,anewresult,newformulasoftransferfunctionandinputimpedancefor2-Ddissipativereactancenetworkcascadesynthesisisgivenintermsofsimplifiedformulastorealizedoublyterminatedresistively1-Ddissipativereactancenetworks.Thenewresultincludesthemoregeneralcases,e.g.thebothcanbedissipativeoroneisdissipativeandtheanotherisalosslessreactancesubnetworkandsoon.Twocalculationexamplesaregiveninthepapertoillustratetheaboverealizations.Besides,consideringsomeproblemsofthedirectconnectionbetweentwosubnetworks,wealsointroduceone-waylinestodiscussthecascadesynthesisofnonreciprocal2-Ddissipativereactancenetworks.

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简介：基于夫妇波浪方程和有限差别的时间域(FDTD)算法，2-Dphotonic水晶couplers的强壮的夫妇特征被计算。理论分析和数字模仿的结果显示在2-Dphotonic水晶coupler的精力不能完全在二波导之间被转移。与弱联合理论的结果相比，我们的结果是更精确的。CLC数字TN252文件代码A由福建省(2001J008)的青春科技革新条款基础支持了

简介：SDS-PAGE将SDS所带的大量阴性电荷和聚丙烯酰胺凝胶的高分辨率相结合,使蛋白自身的电荷被大量的阴性电荷所淹没,这样蛋白在电泳时的迁移率仅与其分子大小有关。蛋白电泳时凝胶的孔径大小和电泳介质对不同大小分子的分辨率有不同的影响,特别对相对分子量小于10000的低分子量蛋白,常规的电泳方法完全不能分辨,国外报道的尿素、缓冲液浓度及聚丙烯酰胺中的单体和双体的比例对蛋白的分辨率有较大的影响,但将几种不同因素进行综合研究,未见报道。

简介：Superconvergenceofthemixedfiniteelementmethodsfor2-dMaxwellequationsisstudiedinthispaper.Twoorderofsuperconvergentfactorcanbeobtainedforthek-thNedelecelementsontherectangularmeshes.

简介：Astochastictwo-dimensionalFornasini-Marchesini'sModelⅡ(2-DFMMⅡ)withmultiplicativenoiseisgiven,andafilteringalgorithmforthismodel,whichisoptimalinthesenseoflinearminimum-variance,isdeveloped.Thestochastic2-DFMMⅡwithmultiplicativenoisecanbereducedtoa1-Dmodel,andtheproposedoptimalfilteringalgorithmforthestochastic2-DFMMⅡwithmultiplicativenoiseisobtainedbyusingthestateestimationtheoryof1-Dsystems.Anexampleisgiventoillustratethevalidityofthisalgorithm.

简介：Theboundaryelementmethodisusedforthemodalanalysisoffreevibrationof2-Dcompositestructuresinthispaper.Sincetheparticularsolutionmethodisusedtotreatthetermsofbodyforces(inertialforces)intheequationofmotion,onlystaticfundamentalsolutionsareneededinsolvingtheproblem.Foranisotropiccantileverbeam,thenumericalresultsobtainedbyusingtheBEMpresentedinthispaperareingoodagreement,withthoseofusingFEMorotherBEM,butthisBEMcanalsobeusedtoanalyzeproblemsforanisotropicmaterials.Forsimplysupportedcompositelaminatedbeams,thecomparisonsofthenumericalresultsobtainedbythismethodwiththeanalyticalresultsobtainedby1-Dlaminatedbeamtheoryindicatethatiftheratiooflength/thicknessisgreaterthan20,theresultsofthetwomethodsareingoodagreement,butiftheratiooflength/thicknessislessthan20,bigerrorswilloccurfor1-Dlaminatedbeamtheory.

简介：AfiniteelementmethodforthesolutionofOseenequationinexteriordomainisproposed.Inthismethod,acircularartificialboundaryisintroducedtomakethecomputationaldomainfinite.Then,theexactrelationbetweenthenormalstressandtheprescribedvelocityfieldontheartificialboundarycanbeobtainedanalytically.Thisrelationcanserveasanboundaryconditionfortheboundaryvalueproblemdefinedonthefinitedomainboundedbytheartificialboundary.Numericalexperimentispresentedtodemonstratetheperformanceofthemethod.

简介：Inthispaper,ahorizontal2-Dnumericalmodelhasbeendevelopedtosimulateflowprocessesindikeburst.Thefinitedifferencemethodisusedincomputation.Themodelemploys2-Dflowequationsandcansimulatecomplexflowswhensupercriticalflowandsub-criticalflowexistsimultaneouslysuchashydraulicjumps.Severalsimulatedresultsareworkedouttodemonstratetheapplicabilityofthenumericalmodel,suchasfloodpropagationonadrybedofacomplexterrain.

简介：TheproblemsofcharacteristicpolynomialassignmentinFomasini-Marchesini(F-M)modelⅡof2-Dsystemsareinvestigated.Thecorrespondingclosed-loopsystemsdescribedbyF-MmodelIIareobtainedviathestatefeedback.Usingthealgebraicgeometrymethod,thecharacteristicpolynomialassignmentinthedosed-loopsystemsisdiscussed.Intermsofthetheoryofalgebraicgeometry,theproblemofcharacteristicpolynomialassignmentistransferredtotheonewhetherarationalmappingisonto.SufficientconditionsforalmostarbitraryassignmentcoefficientsofcharacteristicpolynomialinF-MmodelⅡof2-Dsystemsviastatefeedbackarederived,andtheyareavailableformulti-inputcases.Italsohasbeenshownthatthismethodcanbeappliedtoassignthecharacteristicpolynomialwithoutputfeedback.Thesufficientconditionsforalmostarbitraryassignmentcoefficientsofcharacteristicpolynomialofmulti-input2-DsystemsdescribedbyF-MmodelⅡwithoutputfeedbackareestablished.

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简介：Theauthorsprovetheglobalexistenceofweaksolutionsto2-DincompressibleNavier-Stokesequations(invorticity-streamformulation)withiaitialvorticityinL^4/5.ItmaybethebestresultthatcanbeobtainedforinitialvorticityinL^pform.Moreover,theuniqueneasistobeprovedhere.

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简介：Itisrevealedthatthedynamicstabilityof2-Drecursivecontinuous-discretesystemswithintervalparametersinvolvestheproblemofrobustHurwitz-Schurstabilityofbivariatepolynomialsfamily.ItisprovedthattheHurwitz-Schurstabilityofthedenominatorpolynomialsofthesystemsisnecessaryandsufficientfortheasymptoticstabilityofthe2-Dhybridsystems.The2-Dhybridtransformation,i.e.2-DLaplace-Ztransformation,hasbeenproposedtosolvethestabilityanalysisofthe2-Dcontinuous-discretesystems,togetthe2-Dhybridtransferfunctionsofthesystems.TheedgetestfortheHurwitz-Schurstabilityofintervalbivariatepolynomialsisintroduced.TheHurwitz-Schurstabilityoftheintervalfamilyof2-Dpolynomialscanbeguaranteedbythestabilityofitsfiniteedgepolynomialsofthefamily.Analgorithmaboutthestabilitytestofedgepolynomialsisgiven.

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简介：ＴＨＥ２－ＤＮＵＭＥＲＩＣＡＬＳＴＵＤＹＯＮＴＨＥＰＲＩＮＣＩＰＬＥＳＯＦＲＡＩＮ－ＥＮＨＡＮＣＥＭＥＮＴＡＮＤＨＡＩＬ－ＳＵＰＰＲＥＳＳＩＯＮＩＮＣＯＮＶＥＣＴＩＶＥＣＬＯＵＤＳＭａｏＹｕｈｕａ（毛玉华）ａｎｄＨｕＺｈｉｊｉｎ（胡志晋）ＴＨＥ２－Ｄ...