简介：Chlamydia,ahumanpathogenthatmediateschronic,persistentandrepeatedinfectionprogress,iscapableofinflictingpermanentscarformation.Atypicalreticularbodyisfoundinpersistentinfections.ChlamydialHsp60,interferon(IFN)-gammaandothercytokinesfunctioninthecourseofrepeatedinfection.Immunopathologicalfactorsmediatechronicinfection.

简介：Objective:Forthepurposeofunderstandingthechangingprocessofsyphilishistomorphologyanditsinjurymechanism,theultrastructureofdermatictissuesofsecondarysyphiliswasstudied.Methods:Differentskininjurytissuesofsecondarysyphilispatients,whoseserumRPRandTPHAtestsinthelabbothappearedpositivereaction,wereobservedthroughtransmissionelectronmicroscope(TEM).Results:Inflammationsappearedonepidermidesandcoria,agreatdealofneutrocytes,lymphocytesandasmallamountofplasmainfiltratedthem.Karyopyknosis,karyorrhexis,epicytelysisandmitochondrionvaculardegenerationoccurred.Spirochetapallidawasdistributedonintercellularsubstances,epicytesandcollagenousfibers.Theepicyteswerepressedtofoveation.ConclusionThepathologicalchangeofcharacteristictissueultrastructurereportedhereisahistomorphologicalfoundationtostudytheorganisminjurymechanismcausedbvsvnhilis.

简介：Objective:ToinvestigatetheserotypesandauxotypesdistributionofNeisseriagonorrhoeaeinGuangzhou.Method:131strainsofNeisseriagonorrhoeaewereserotypedbyco-agglutinationtestand108strainswereauxotypedbyLaScolea'smethod.Results:Outof131strainsofNeisseriagonorrhoeae,87.8%(115/131)wereWⅡ/WⅢ,while9.9%(131131)wereWI.ThemostimportantauxotypeswereProto,ProandILe,42.6%(46/108),21.3%(23/108)and12.0%,respectively.WII/WIIIwasdistributedamongtheallauxotypesaboveandWⅠfoundonlyinbothProtoandPro.Conclusion:Thestudyillustratedtheprevailingserotype,WⅡ/WⅢ，andhigherprevalenceofIle-inGuangzhou.

简介：Abstract:ChlamydiaTrachomatis(C.T.)isoneofthemostcommonpathogensofhumansexuallytransmitteddiseases.TreatmentofC.T.infectionprimarilydependsonTetracyclines,MacrolidesandQuinolones,butwiththewideuseofantibioticsanincreasingnumberofdrug-resistantChlamydiatrachomatiscaseshavebeenreported.ThisreviewsummarizestheresistantconditionsandthepossibleresistancemechanismsofC.T..

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简介：Object:Toinvestigatetherelationshipbetweenchlamydiatrachomatis(CT)andurogenitalinfection.Method:PositiverateofCTinpatientswithinflammationofurogenitaltractwassignificantlyhigherthanthosewithoutinflammation(P<0.05).Result:Therewasstatisticaldifferenceinthemalesnomattertheywerepatientswithinflammationofurogenitaltractornot(P>0.05),whiletherewasnostatisticaldifferenceinfemales(P>0.05).Theincidenceoftheinfectionwashighamongthoseagingfrom21-50yearsold.Conclusion:TheclinicalmanifestationsofCTinfectionwereobscure,soweshouldexamineCTinpatientswhohavenosymptoms,especiallyinfemalesandthoseofhigh-riskpopulation.

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简介：Objective:ToincorporatestudentsandotherlocalcommunitypartnersincreatingacollaborativeNanjingWorldAIDSDayexhibition.Background:StigmaanddiscriminationcontinuetocomplicateeffectiveWorldAIDSDayactivitiesinChina.IncludingforeignstudentsinWorldAIDSDaycampaignsbroadensthepotentialChineseaudienceaswellasmakingthegovernment'sAIDSpoliciesmoretransparent.Methods:Inordertobroadenthepotentialaudience,relevantmedia(television,radio,newspaper)werenotifiedofthe2003NanjingWorldAIDSDayExhibition.TheexhibitionrepresentedauniquecollaborationbetweenNanjingUniversityandtheChineseNationalCenterforSTDandLeprosyControl,dependingonforeignandChinesestudentsatseverallevels.Inaddition,closecontactsattheJiangsuProvincialHealthBureauhelpedtocoordinatelocalexpertsanddistributionofcondoms.Results:Thismulti-disciplinarycooperativeexhibitionwassuccessfullycompleted.Severalmajormediasourcescoveredtheevent.Conclusions:ThesuccessofthiscampaignsuggeststhatotherurbancitieswithsignificantforeignstudentpopulationscouldbenefitfromincludingforeignandChinesestudentsinfutureWorldAIDSDaycampaignactivities.

简介：Objective:ToexploretherelationshipbetweenquantitativeTreponemapallidumDNA(TP-DNA)PCRtestingandtheToludineRedUnheatedSerumTest(TRUST)inpatientswithsyphilisbeforeandaftertreatment,andevaluatetheclinicalvalueofquantitativeTP-DNAtestinginthediagnosisandtreatmentevaluationofsyphilis.Methods:29patientswithprimary(12cases)orsecondary(17cases)syphilis,whometthecriteriasetforthisstudywererecruitedassubjects.Allpatientsweretreatedwith2.4millionunitsbenzathinepenicillinIMweeklyfor3weeks.QuantitativetestsofTP-DNAinthepatients'plasmawereperformedusingFQ-PCRbeforeandafterthetreatment.SerologictestsincludingTRUSTandTPPAwerealsoperformed.Results:Beforethetreatment,9outof12primarysyphilispatients(75%)andallsecondarysyphilispatients(17/17)testedpositiveforTreponemapallidum(TP)byTP-DNAtesting.TheaveragequantitativetestvaluesofTP-DNAinprimaryandsecondarysyphilispatientswere(3.38±2.34)×10^4and(5.73±1.33)×10^6copies/ml,respectively.Afterthreemonthsoftreatment,1ofthe9primaryand5outof17secondarysyphilispatientswerepositiveuponTP-DNAtesting,respectively.TheaveragequantitiesofTP-DNAwere2.01×10^2copies/mlinprimaryand5.87×10^2copies/mlinsecondarysyphilispatientswithpositiveTRUSTandTP-DNAtests,and3.09×10^2copies/mlforthosewithnegativeTRUST,respectively.Afterninemonthsoftreatment,alltheprimaryandsecondarysyphilispatientswerenegativeuponTP-DNAtesting,whileallprimaryand14of17(82.35%)secondarysyphilispatientsshowednegativeTRUSTresults.Conclusion:ThattheresultsofTP-DNAtestsarenotconsistentwiththoseofTRUSTbeforeandaftertreatmentindicatesthatquantitativeTP-DNAtestingmayhavevaluableclinicalsignificanceintheearlydiagnosisandevaluationoftreatmentregimensforsyphilis.

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简介：Objectives:Toevaluatetheefficacyofnestedpolymerasechainreaction(PCR)withfirstvoidurine(FVU)forthediagnosisofMycoplasmahominisinmalepatients.Methods:MatchedFVUspecimensandurethralswabswerecollectedfrom194malepatientswithNongonococcalUrethritisandtestedbynestedPCRandcellculture.Cellculturewasusedasagoldstandardforevaluatingotherassaytechniques.Results:ForFVUnestedPCRassayandFVUcellculture,ourresultsshowedthatthesensitivitywas100%and93.3%;specificitywas97.0%and98.2%;positivepredictivevalue(PPV)was85.7%and90.3%,negativepredictivevalue(NPV)was100%and98.8%,respectively.Thetotalconsistencybetweenthetwotechniqueswas97.4%.Conclusions:ForthediagnosisofMycoplasmahominisinmen,nestedPCRdetectingFVUisahighlysensitiveandspecificmethod.FirstvoidurinecanreplaceswabcultureorPCRintermsofacceptabilityandfeasibility.

简介：Objective:Tostudytheroleofmonocytesinthepathogenesisofgenitalherpes.Methods:TNF-aandIL-6levelsin27casesofgenitalherpesweredetectedbyenzymelinkedimmunosorbantassay(ELISA).HLAclassⅡantigenexpressiononmonocytesweredetectedbyanalkalinephosphataseanti-alkalinephosphatasemethod.Results:Comparedwithnormalcontrols,levelsofTNF-aandIL-6secretedbymonocytesrespondingtoLPSmitogeninvitroweresignificantlydecreased[(3.13±0.44ng/ml)vs(4.68±0.54ng/ml),P<0.05and(3.32±1.06ng/ml)vs(6.46±1.94ng/ml),P<0.05,respectively].HLAclassⅡantigenexpressiononmonocytesinthegenitalherpesgroupwasalsosignificantlydecreased[HLA-DR(67.48%±1.51%)vs(81.03%±1.32%),P<0.01andHLA-DQ(29.54%±1.15%)vs(37.63%±1.79%),P<0.01respectively].Conclusion:Thesefindingssuggestthatthedecreasedmonocytefunctionmaycontributetothepathogenesisofgenitalherpes.Augmentingorinducingmonocytefunctionmaybeimportantintheprevention,treatment,andreductionofgenitalherpescases.