简介:Objective:AllelicpolymorphismsofCCR5△32、CCR2b-64I,CX3CR1-249I280MandSDF1-3’AassociatedwithHIV-1infectionanddiseaseprogressionwereinvestigatedinindigenousUygurpopulationsfromtheXinjiangUygurAutonomousRegionofChina.Mithods:Thestudypopulationcomprised316healthyUygursubjectswithanagerangeof1-80yearsold,fromwhomwholeperipheralbloodsampleswerecollectedandnonewereHIV-1seropositive.GenomicDNAsampleswerepurifiedusingaQiagenBloodKit.GenotypingoftheaforementionedfouralleleswasperformedusingPCRorPCR/RFLPassay,andfurtherconfirmedbydirectDNAsequencing.Results:TheallelicfrequenciesinChineseUygurpopulationwereasfollows:3.48%forCCR5△32;19.45%forCCR2b-64I;13.8%forCX3CRI-249I280Mhaplotype,and20.41%forSDFI-3’A.MutantalleledistributionsamongUygurpopulationswereinaccordancewiththeHardy-Weinbergequilibrium.NostatisticaldifferencewasfoundbetweenthefrequencyofthethreeHIVcoreceptorsandtheirrespectiveligandgenes.Conclusion:ThefrequencyofSDF1-3’AandCX3CR1-249I280MhaplotypemorecloselymatchedthehanChinese.ThefequencyofCCR5△32inUygurpopulationswasbetweenCaucasianandHanfrequencies,themorecloselymatchingthefrequencyinMedi-Asiapeople.NogeneticlinkagebetweenanytwoofthethreeHIVcoreceptorgeneswasfound,butobviousgeneticlinkagesexistedbetweenCX3CR1-249IandCX3CR1-280M,withevenhigherlinkagedegreesthanCaucasianpeople.
简介:Objective:Theaiminthisstudywastoidentifytheallelicfrequenciesofthechemokine(SDF1-3'A)andchemokinereceptor(CCR5△32,CCR5m303andCCR2-64I)genesresistanttoHIV-1infectionand/ordiseaseprogressioninindigenousChinesepopulations.Methods:ByusingQIAampDNABloodMiniKit,thegenomicDNAsampleswerepurifiedfromwholeperipheralbloodofhealthyindividuals(n=2067)fromHan,Uygur,MongolianandTibetanethnicgroups,aswellasHanpatientsincludingHIV-1carriers(n=330),patientswithothersexuallytransmitteddiseases(STDs,n=259)andintravenousdrugusers(IVDUs,n=125).TheallelicpolymorphismswereidentifiedbymeansofPCRorPCR-RFLPanalyses.ThesequencesofrandomlyselectedamplifiedPCRproductswerefurtherconfirmedbydirectDNAsequencing.Results:Themutantfrequencieswereidentifiedtobe0%-3.48%forCCR5△32,0%forCCR5m303,19.15%-28.79%forCCR2-64and19.10%-28.73%forSDF1-3'Aalleles,respectively,inChinesehealthyindividualsfromfourethnicgroups.Ourfindingsindicatedtheallelicfrequenciesvaryamongthedifferentethnicgroups.Furthermore,theHIV-1carriers,STDcasesandIVDUs(allofHanethnicity)werefoundtohavetheallelicfrequenciesof0%-0.19%(CCR5A32),0%(CCR5m303),19.31%-20.45%(CCR2-64)and25.61%-26.83%(SDF1-3'A)withminorvariationsintheirfrequenciesbetweenthepatientsandhealthyHangroups.TherewasnoCCR5-m303mutationfoundinanysubjectinthisstudy.Conclusion:TheexaminedsubjectsoffourChineseethnicoriginsshowedlowerfrequenciesofCCR5A32andCCR5m303alleles,buthigherfrequenciesofmutantCCR264IandSDF1-3'Aallelescomparedtothoseidentifiedinnorthern-EuropeanandAmericanCaucasians.ThesignificanceofthedifferentfrequenciesandpolymorphismsoftheaboveallelesinChinesepopulationsneedstobefurtherexaminedinHIV-1/AIDSdiseases.
简介:目的探讨应用水射流辅助吸脂术行全身大容量吸脂的临床效果和安全性。方法对我科32例患者、共139个部位行水射流辅助吸脂,记录手术时间、注入肿胀液量、冲洗液量、吸出总液体量、吸出脂肪量、术后疼痛时间,观察吸出物颜色、术中术后患者生命体征、皮肤瘀斑和水肿的程度、皮肤平整度以及利多卡因毒性反应等并发症出现情况。结果本组患者平均手术时间4.5h,平均吸脂4.8个部位,平均注入肿胀液量1850ml,平均注入冲洗液量15760ml,平均吸出总液体量11480ml,平均吸脂量4720ml,平均疼痛恢复时间5天。术后6个月恢复良好,无并发症发生。结论水射流辅助吸脂对神经和血管的损伤小,组织肿胀轻,手术效果较好。
简介:目的:探讨耻骨下区V—Y成形阴茎延长术的临床效果。方法:采取耻骨下区V—Y成形阴茎延长术治疗阴茎短小症85例。结果:术后阴茎自然显露长度可延长3~6cm,随访2~3个月,阴茎无明显回缩,患者对手术效果均表满意;术后阴茎根部稍下移,自觉勃起角度降低,但仍〉90度;2例出现皮下脂肪液化,阴茎出现部分回缩;3例出现阴茎根部切口裂开;部分患者术后包皮水肿1~2个月后逐渐消褪。结论:耻骨下区V—Y成形阴茎延长术治疗阴茎短小症临床效果满意。
简介:目的探讨雷公藤多甙片或阿维A胶囊联合静脉滴注复方甘草酸单铵S氯化钠注射液治疗皮肤扁平苔藓的疗效比较。方法根据不同治疗方法将患者分3组。A组:复方甘草酸单铵S氯化钠注射液200mL/d静脉滴注及皮损处中药封包,10~20d为1个疗程。B组:阿维A胶囊20~40mg/d[相当于0.5mg/(kg·d)]口服,余治疗同A组。C组:雷公藤多甙片40~60mg/d[相当于0.8mg/(kg·d)]口服,余治疗同A组。结果A、B、C组治疗10d后,有效率分别为40.00%、63.33%和75.00%。C组有效率高于A组和B组,A组和C组间差异有统计学意义(P〈0.05)。治疗20d后,有效率分别为70.00%、93.33%和93.75%。C组有效率高于A组和B组,B组和C组间差异无统计学意义(P〉0.05)。结论3种治疗方案治疗皮肤扁平苔藓均有效。但B组、C组疗程短,疗效佳。C组起效快,B组和C组差异无统计学意义。
简介:寻常狼疮是皮肤结核中最常见的类型,约占所有皮肤结核患者的半数。本病易侵犯儿童和青少年,好发于面部,影响容貌。我们用异烟肼、乙胺丁醇和利福平联合治愈寻常狼疮2例,报告如下。
简介:目的研究抗FcεRⅠα自身抗体在慢性自发性荨麻疹(Chronicspontaneousurticaria,CSU)中的表达及临床意义。方法采用酶联免疫吸附法(ELISA)检测CSU患者中抗FcεRⅠα自身抗体的表达,并分析抗FcεRⅠα自身抗体与CSU患者的病程、荨麻疹活动性评分(UAS)评分、风团数目、瘙痒程度、生活质量评分、单一抗组胺药疗效的临床相关性。结果CSU患者组抗FcεRⅠα抗体水平较正常对照组明显升高(P〈0.05),抗FcεRⅠα抗体水平与病程、荨麻疹活动性评分(Urticariaactivityscore,UAS)、风团数目评分、瘙痒程度评分、生活质量评分无明显相关性(P〉0.05),单一抗组胺药使用有效者其血清抗FcεRⅠα抗体滴度低于单一抗组胺药治疗无效者(P〈0.05)。结论抗FcεRⅠα抗体在CSU发病中有重要的作用,抗FcεRⅠα抗体滴度较高的CSU患者可能对常规抗组胺药治疗抵抗。
简介:Inordertoacknowledgethemulti-infectiondataofSTDpatientsandimprovetheprophylaxisandtherapyforSTDs,297patientsand30healthypeoplewereexaminedusingtheHSV-2plasmaantibody.TheresultsshowedthatallkindsofSTDpatientswereinfectedbyHSV-2indifierentratios.thehighestratiooccurringinsyphilispatients.
简介:Objective:TounderstandthechangeofTh1/Th2andTc1/Tc2balanceinpatientswithsecondaryearlysyphilis.Methods:PeripheralbloodThandTccellsubpopulationswereexaminedbythree-colorflowcytometryin30patientswithsecondaryearlysyphilisand20healthysubjects.Results:PercentagesofCD3^+,CD4^+TcellsandtheratioofCD4^+/CD8^+andNKcellsinsyphiliticpatientsweresignificantlylowerthaninthecontrolgroup(P<0.001),butnostatisticaldifferencewasfoundinpercentagesofCD8^+andCD19^+cellsbetweenthetwogroups(P>0.05).ThepercentageofTh1cellsandratiosofThl/Th2andTcl/Tc2insyphiliticpatientswereclearlydecreasedascomparedtothoseincontrois(P<0.001-0.01),whereaspercentagesofTc0andTc2cellsinsyphiliticswashigherthanthoseincontrols(P<0.001).Conclusion:Therecouldbeadisturbanceofcellularimmunityandtherelativelyintactfunctionofhumoralimmunityinsecondarysyphilis.ItispostulatedthatthepolarizationtowarddevelopmentofTh2andTc2cellsandtheirassociatedcytokinesmightbeinvolvedintheincompleteclearanceoftreponemesandthedevelopmentofchronicinfection.
简介:Objective:Toconstructarecombinantplasmidcontainingtheoutermembraneprotein2(Omp2)geneofChlamydiatrachomatisandexpressOmp2inE.coli.Methods:Theomp2geneofC.trachomatisserovarDwasclonedintopQE30vectorfollowingPCRamplificationfromgenomicDNA.E.coliM15transformantswereinducedtoexpressthefusionproteinbyIPTGandtheproductwasidentifiedbySDS-PAGEandWesternblot.Results:ConfirmedbyenzymecleavageanalysisandDNAsequencing,acorrectrecombinantplasmidpQE30/omp2wasconstructed.Thefusionproteinfromthetransformantswasapproximately60kDainsizeinSDS-PAGEanalysis,whichcouldspeciallyreactwithanti-6×HismousemonoclonalIgGantibodies.Conclusion:WesuccessfullyexpressedOmp2inE.coilM15,providinganefficientandsimplesystemforassayingtheimmunologicalpropertiesofOmp2.
简介:Objective:Tostudythecellularimmunitystatusofpatientswithrecurrentgenitalherpes.Methods:Serumlevelsofinterlukin-2anditssolublereceptorandinterlukin-6weremeasuredbyELISAin34patientswithrecurrentgenitalherpes.Results:SerumlevelsofIL-2andIL-6weresignificantlydecreasedinpatientscomparedtohealthycontrols(P<0.01),andthelevelofsIL-2Rwassignificantlyincreasedinpatientswithrecurrentgenitalherpes(P<0.01).Therewerenosignificantdifferencesinallvariablesamongstpatientsregardingrelapsestageandremissionstage(P>0.05).Conclusion:Therewasacellularimmunedeficiencyinpatientswithrecurrentgenitalherpes.
简介:ObjectivesTocomparetheefficiencyoffourcommercialELISAkitsindetectingtype-specificHSV-2IgGantibodies.MaterialsandMethods:Atotalof125subjects,including105withgenitalulcers,and20controlswithoutanyhistoryofSTDswererecruitedfromtheSTDclinicfordetectionoftype-specificHSV-2IgGantibodywithdifferentkits.FourkindsofcommerciallyavailableELISAkits,includingQuidaHSV2IgGELISA(AifulangBiochemCo.Ltd.,Hangzhou),TORCH-HSV2IgG(JingmeiBiotechCo.Ltd.,Shanghai),Captia^TMHSV2IgG(Trinitybiotech,USA)andHerpeSelectT^TM2ELISAlgG(Focustechnologies,USA)wereusedforevaluation.WesternBlotassaywasperformedasagoldstandard.Results:ComparedtoWesternBlotresults,thesensitivityandspecificityofthekits(QuidaHSV2,TORCHHSV2,Captia^TMHSV2andHerpeSelect^TM2)were13.1%and98.4%,7.5%and100%,100%and11.1%,87.7%and96.7%,respectively.Thepositivepredictivevalue(PV)andnegativePVofthefourkitswere88.9%and54.3%,100%and55.5%,55.6%and100%,96.2%and89.2%,respectively.TheareasundertheROCcurveofthreekits(QuidaHSV2,Captia^TMHSV2andHerpeSelect^TM2)were0.885(0.822-0.948),0.825(0.747-0.902),0.974(0.950--0.998),respectivelyConclusion:TheperformanceofHerpeSelect^TM2isthebestamongthefourkits.Theresultsalsoindicatethatthecommerciallyavailablekitsfordetectionoftype-specificHSV2antibodyshouldbere-evaluatedintermsoftheirvalidity.
简介:Objective:Toinvestigatetherolesofinterleukin-2(IL-2)andinterleukin-10(IL-10)inpathogenesisofearlysyphilis.Methods:TheserumlevelsofIL-2andIL-10in48patientswithearlysyphilisweredetectedbyABC-ELISA.Results:(1)ThelevelofIL-2inthepatientswithearlysyphiliswassignificantlyhigherthanthatinhealthycontrols,whilethatofIL-10waslower(P<0.001andP<0.001).(2)ThelevelsofIL-2andIL-10werealmostidenticalinpatientswithprimaryandsecondarysyphilis(P>0.05),aswellasbetweendif-ferentRPRtiters(P>0.05).(3)Aftertherapy,thelevelofIL-2decreasedmarkedly(P<0.05),whilethatofIL-10increase(p>0.05).(4)AsignificantcorrelationwasfoundbetweentheserumlevelsofIL-2andIL-10(r=0.5385P<0.05).Conclusions:Th1up-regulationoccursinpatientswithearlysyphilis,andplaysanactiveroleinfightingagainstTPinfection.