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简介：Objective:Toclonemultidrugresistance(MDR)relatedgenesinlungadenocarcinomacelllines.Methods:ThedifferentiallyexpressedcDNAfragmentsbetweenA549andA549DDPcellswereanalyzedbymRNAdifferentialdisplayPCR(DDRT-PCR).ThefragmentsthusobtainedwerefurtheranalyzedbyDNAsequencingandNorthernblotting.Results:ThreedifferentiallyexpressedcDNAfragmentswereobtainedandconfirmedbyNorthernblot.Sequenceanalysisrevealedthattwoofthemwerenovelandonewas100%identicalwithICEgene.Conclusion:AnalyzingdifferentiallyexpressedfragmentbetweenA549andA549DDPcellsmaybehelpfulforfindingnewMDRrelatedgenes.ThedrugresistanceofA549DDPcellsmayberelatedtotheinhibitionordown-regulationofICEgene.

简介：Rat-1cellsweretransfectedwithDNAfromhumanesophagealcancer2K,4K,6K,7K.8K.Thetransformingfociwereobtainedandthetransformingcelllineswereestablished.Thecelllinescanformlargercolonyinsoftagar.Thosenudemiceinjectedsubcutaneouslywiththecellssufferedfromlargerfibroussarcoma.Thisindicatesthatthecelllineshavecarcinogenicity.TheexperimentalresultssuggestthathumanDNAsequenceandhumanHa-rasspecial616Kb(BamHI)bandarepresentintheDNAofthetransformingcells.Theover-expressionofrasgeneproductsP21werefoundinthetissuesofexophagealcancer,thetissuesadjacenttotumorandthetransformingcells.

简介：Objective:Livermetastasis,whichcontributessubstantiallytohighmortality,isthemostcommonrecurrentmodeofcoloncarcinoma.Thus,itisnecessarytoidentifygenesimplicatedinmetastaticcolonizationoftheliverincoloncarcinoma.Methods:WecomparedmRNAprofilingin18normalcolonmucosa(N),20primarytumors(T)and19livermetastases(M)samplesfromthedatasetGSE49355andGSE62321ofGeneExpressionOmnibus(GEO)database.Geneontology(GO)andpathwaysoftheidentifiedgeneswereanalyzed.Co-expressionnetworkandproteinproteininteraction(PPI)networkwereemployedtoidentifytheinteractionrelationship.SurvivalanalysesbasedonTheCancerGenomeAtlas(TCGA)databasewereusedtofurtherscreening.Then,thecandidategeneswerevalidatedbyourdata.Results:Weidentified22specificgenesrelatedtolivermetastasisandtheywerestronglyassociatedwithcellmigration,adhesion,proliferationandimmuneresponse.Simultaneously,theresultsshowedthatC-X-Cmotifchemokineligand14(CXCL14)mightbeafavorablepredictionfactorforsurvivalofpatientswithcoloncarcinoma.Importantly,ourvalidateddatafurthersuggestedthatlowerCXCL14representedpooreroutcomeandcontributedtometastasis.Genesetenrichmentanalysis(GSEA)showedthatCXCL14wasnegativelyrelatedtotheregulationofstemcellproliferationandepithelialtomesenchymaltransition(EMT).Conclusions:CXCL14wasidentifiedasacrucialanti-metastasisregulatorofcoloncarcinomaforthefirsttime,andmightprovidenoveltherapeuticstrategiesforcoloncarcinomapatientstoimproveprognosisandpreventmetastasis.

简介：Objective:Toidentifydifferentiallyexpressedlongnon-codingRNAs(lncRNAs)involvedinthemetastasisofepithelialovariancancer.Methods:AninvitroinvasionassaywasperformedtovalidatetheinvasivecapabilityofSKOV3andSKOV3.ip1celllines.TotalRNAwasthenextracted,andmicroarrayanalysiswasperformed.Moreover,ninelncRNAswereselectedforvalidationusingRT-qPCR.Results:ComparedwiththeSKOV3cells,theSKOV3.ip1cellssignificantlyimprovedintheinvitroinvasiveactivity.Ofthe4,956lncRNAsdetectedinthemicroarray,583and578lncRNAswereupregulatedanddownregulated,respectively,inSKOV3.ip1cells,comparedwiththeparentalSKOV3cells.SevenoftheanalyzedlncRNAs(MALAT1,H19,UCA1,CCAT1,LOC645249,LOC100128881,andLOC100292680)confirmedthederegulationfoundbymicroarrayanalysis.Conclusion:LncRNAsclustersweredifferentiallyexpressedinovariancancercellswithvaryingmetastaticpotentials.ThisresultindicatesthatsomelncRNAsmightexertapartialorkeyroleinepithelialovariancancermetastasis.FurtherstudiesshouldbeconductedtodeterminetherolesoftheselncRNAsinovariancancermetastasis.

简介：Objective:Multiplemechanismsunderlyingthedevelopmentofportalveintumorthrombus(PVTT)inhepatocellularcarcinoma(HCC)havebeenreportedrecently.However,theoriginsofPVTTremainunknown.Increasingmulti-omicsdataonPVTTsinHCCshavemadeitpossibletoinvestigatewhetherPVTTsoriginatefromthecorrespondingprimarytumors(Ts).Methods:TheclonalrelationshipbetweenPVTTsandtheircorrespondingprimaryTswasinvestigatedusingdatasetsdepositedinpublicdatabases.OneDNAcopynumbervariationsdatasetandthreegeneexpressiondatasetsweredownloadedfortheanalyses.ClonalityanalysiswasperformedtoinvestigatetheclonalrelationshipbetweenPVTTsandTsfromanindividualpatient.DifferentialgeneexpressionanalysiswasappliedtoinvestigatethegeneexpressionprofilesofPVTTsandTs.Results:Oneoutof19PVTTshadnoclonalrelationshipwithitscorrespondingT,whereastheothersdid.ThePVTTswithindependentclonaloriginshoweddifferentgeneexpressionandenrichmentinbiologicalprocessesfromtheprimaryTs.Basedontheuniquegeneexpressionprofiles,agenesignatureincluding24geneswasusedtoidentifypairsofPVTTsandprimaryTswithoutanyclonalrelationship.ValidationinthreedatasetsshowedthatthesetypesofpairsofPVTTsandTscanbeidentifiedbythe24-genesignature.Conclusions:OurfindingsshowadirectevidenceforPVTToriginandconsolidatetheheterogeneityofPVTTsobservedinclinic.TheresultssuggestthatPVTTinvestigationatamolecularlevelisclinicallynecessaryfordiagnosisandtreatment.

简介：Monoclonalantibody(MAb)toratlivercyto-chromeP-450jisozyme,anactivatingenzymespecifictonitrosaminemetabolism,wasusedcoupledwithimmunoblotting,densitometerscanningofSDS-PAGEgelsandimmunohistochemicaltechnique.ThetraceP-450HSjisozyme(Mr.51.5Kd)wasfoundinhumangastricmucosa.ItwassimilartoP-450jinmolecularweight,catalyticandimmunochemicalproperties.TheconcentrationsofP-450HSjinmucosaoflessercurvaturewerehigherthanthoseingreatercurvature.Thismightbeoneoftheimportantreasonsthatlessercurvatureisthecommonestareaforgastriccarcinoma.ButtherewaspossiblylessP-450HSjingastricmucosawithcancer.Im-munohistochemically,P-450HSjwasdiscoveredinthecytoplasmofsomeglandularepithelialcells,especiallyintheglandswithhyperplasticandintestinalmetaplasticchangesadjacenttocarcinoma.Itwasalsofoundinsomenormalglandsandintumorcellsofhigh-differentiatedadenocarcinoma,butnotinthoseoflow-differentia

简介：AJNR,2008Jun26.BACKGROUNDANDPURPOSE:InclusionofoligodendroglialtumorsmayconfoundtheutilityofMRbasedgliomagrad-ing.Ouraimwas,first,toassessretrospectivelywhetherahistogram-analysismethodofMRperfusionimagesmaybothgrade

简介：Objective:ToevaluatethefeasibilityofDNAimagecytometry(DNA-ICM)asaprimaryscreeningmethodforesophagealsquamouscellcancer(ESCC).Methods:Atotalof5,382localresidentsaged40–69yearsfromthreehigh-riskareasinChina(LinzhouinHenanprovince,FeichenginShandongprovinceandCixianinHebeiprovince)from2008to2011wererecruitedinthispopulation-basedscreeningstudy.And2,526subjectsdeclinedtoreceiveendoscopicbiopsyexaminationwithLugol'siodinestaining,while9and815subjectswereexcludedfromliquid-basedcytologyandDNA-ICMtestrespectivelyduetoslidequality.Finally,2,856,5,373and4,567subjectswereenrolledintheanalysisforendoscopicbiopsyexamination,liquid-basedcytologyandDNA-ICMtest,respectively.Sensitivity(SE),specificity(SP),negativepredictivevalues(NPV)andpositivepredictivevalues(PPV)aswellastheir95%confidenceintervals(95%CI)forDNA-ICM,liquid-basedcytologyandthecombinationofthetwomethodswerecalculated.Receiveroperatingcharacteristic(ROC)curveswereappliedtodeterminethecutoffpointofDNA-ICMforesophagealcancer.Results:DNA-ICMresultsweresignificantlycorrelativewithesophagealcancerandprecancerlesions(χ~2=18.016,P<0.001).Thecutoffpointswere5,802,5,803and8,002basedondissimilarpathologicaltypesoflowgradeintraepithelialneoplasia(LGIN),highgradeintraepithelialneoplasia(HGIN),andESCC,respectively,and5,803waschoseninthisstudyconsideringtheSEandSP.TheSE,SP,PPV,NPVofDNA-ICMtest(cutoffpoint5,803)combinedwithliquid-basedcytology[thresholdatypicalsquamouscellsofundeterminedsignificance(ASCUS)]wereseparately72.1%(95%CI:70.3%-73.9%),43.3%(95%CI:41.3%-45.3%),22.8%(95%CI:21.1%-24.5%)and87.0%(95%CI:85.7%-88.3%)forLGIN,85.7%(95%CI:84.3%-87.1%),41.3%(95%CI:39.3%-43.3%),4.6%(95%CI:3.8%-5.4%)and98.9%(95%CI:98.5%-99.3%)forHGIN,and96.0%(95%CI:95.2%-96.8%),40.8%(95%CI:38.8%-42.8%),1.7%(95%CI:1.2%-2.2%)and99.9%