简介：ThepresentstudywasdesignedtoinvestigatethechemicalconstituentsoftherootsofCudraniafruticosaWight.Compoundswereisolatedbyvariouscolumnchromatographicmethodsincludingsilicagel,polyamide,sephadexLH-20,andsemi-preparativeHPLC.Theirstructureswereelucidatedbyacombinationof1Dand2DNMRtechniques,massspectrometry,andchemicalmethods.Twonewxanthones,CudraxanthoneTandU(1-2),alongwithfourknowncompounds(3-6)wereisolatedfromtherootsofCudraniafruticosaWight.

简介：Twonewcompounds,(22E)-25-carboxy-8β,14β-epoxy-4α,5α-dihydroxyergosta-2,22-dien-7-one(1)andfusidione(3),alongwithtwoknowncompounds,5α,8α-epidioxyergosta-6,22-diene-3β-ol(2)andmicroperfuranone(4),wereisolatedfromthefermentationproductsofthemarine-sourcedfungusAcremoniumfusidioidesRZ01.Thestructuresofcompounds1and3wereelucidatedbyextensivespectroscopicmethods,especially2DNMR,andtheirabsoluteconfigurationsweresuggestedonthebasisofthecirculardichroismspectralanalysisandtheNOESYdata.BothnewcompoundsshowedinhibitoryactivityagainstHL-60cellswithIC50valuesbeing16.6and44.9μmol·L-1,respectively.

简介：Maca(Lepidiummeyenii)是在高高原成长的草本的植物并且因为它人的健康的好处，几个世纪被用作食物和民间药。在现在的学习，它的(内部抄录分隔符)43件maca样品定序，从不同区域或供应商镇定，被放大并且分析。它maca的十九潜在的掺杂物的序列也镇定、分析。结果显示maca的ITS顺序在所有样品是一致的并且唯一什么时候与它的掺杂物相比。因此，这条DNA-barcoding途径基于它的顺序能被用于maca和它的掺杂物的分子的鉴定。

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简介：Sesquiterpene内酯在他们的特殊结构和活动的优点在Kudiezi注射被看作主要活跃混合物。此处，一个分析方法为用结合的高效的液体层析的在Kudiezi注射的快速的屏蔽和sesquiterpene内酯的鉴定被开发在否定离子模式的线性离子trap-orbitrap团spectrometry(HPLC-LTQ-Orbitrap)。首先，二个sesquiterpene内酯参考标准被分析获得他们的典型ESI-MS/MS破碎模式。第二，基于提取离子层析(EIC)数据挖掘方法和典型破碎小径分析，在Kudiezi注射的sesquiterpene内酯很快被屏蔽并且识别。最后，一个重要参数堵塞P被采用区别sesquiterpene内酯的异构体。作为结果，50sesquiterpene内酯被描绘，包括9sesquiterpene内酯aglycones，39sesquiterpene内酯glycosides，和2氨基的acid-sesquiterpene内酯结合。在他们之中，13混合物尝试性地作为新混合物被识别。结果证明确定的方法将是为在自然的药的复杂系统屏蔽和sesquiterpene内酯的鉴定的一个快速的、有效分析工具。

简介：Si-Miao-Wan(SMW),atradiationalChinesemedicinalformulaconsistingofAtractylodisRhizoma,PhellodendriChinensisCortex,CoicisSemen,andAchyranthisBidentataeRadix,hasbeenusedforthetreatmentofgoutandgoutyarthritisformanyyears.Inthepresentstudy,aliquidchromatographyquadrupole-time-of-flightmassspectrometry(LC-Q-TOF/MS)methodwasestablishedtoidentifythemultipleconstituentsofSMWanditsmetabolitesinratbiologicalsamplesafteroraladministration.Atotalof48compoundsinSMW,including21alkaloids,12organicacids,2terpenes,3lactones,2phytosterols,and8othercompounds,weretentativelycharacterizedwiththediagnostic-ionfilteringstrategy.BasedonthediagnosticionsappliedtoidentifycompoundsinSMW,28prototypecompoundsand10metaboliccompoundsweredetectedinthebiologicalsamples.ThiswasthefirstcomprehensivedrugmetabolisminvestigationofSMWinrats.Thedevelopedmethodcouldbeausefulmeansforidentifyingthemulti-componentsinSMWandthemetaboliccomponents.TheresultsmayhelpexplorethepossiblemetabolicprocessesandmechanismofactionforSMWinvivo.

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简介：TocharacterizeandidentifymultipleconstituentsinDanhonginjection(DHI),afastultra-highperformanceliquidchromatographycoupledtoelectrosprayionizationquadrupoletime-of-flighttandemmassspectrometry(UHPLC-ESI-QTOF/MS)methodwasestablishedandvalidatedinthepresentstudy.Atotalof63compounds,including33phenolicacids,2C-glycosylquinochalcones,6flavonoidO-glycosides,4iridoidglycosides,6organicacids,5aminoacids,and3nucleosides,wereidentifiedortentativelycharacterized.Inconclusion,theUHPLC-ESI-QTOF/MSmethodisusefulandefficientforin-depthstructuralelucidationofchemicalcompoundsincomplexmatricesofherbalmedicinessuchasDHI.

简介：ThefruitsofPaulowniacatalpifoliaGongTongareusedasaChinesefolkherbalmedicineforthetreatmentofenteritis,tonsillitis,bronchitis,anddysentery,etc.OurpreviousstudyhasidentifiednewC-geranylatedflavanoneswithobviousanti-proliferativeeffectsinlungcancerA549cells.Inthepresentstudy,anewC-geranylatedflavone,paucatalinoneC(1)andfiveknownC-geranylatedflavanones(2-6)wereisolated.Inaddition,atotalof34C-geranylatedflavonoidsweredetectedbyHPLC-DAD-ESI-MS/MScouplingtechniquesfromtheCH_2Cl_2extractofP.catalpifolia.Futhermore,anti-agingeffectsofisolatedcompoundswereevaluatedinvitrowithprematuresenescent2BScellsinducedbyH_2O_2.PhytochemicalresultsindicatedthatP.catalpifoliawasanaturalresourceofabundantC-geranylatedflavonoids.Diplacone(3)andpaucatalinoneA(5)werethepotentanti-agingagentsintheprematuresenescent2BScellsinducedbyH_2O_2andtheC-geranylsubstituentmaybeanimportantfactorbecauseofitslipophiliccharacter.