简介:目的分析和比较应用射频消融房室结慢径治疗房室结折返性心动过速对老年人和青年人房室结电生理功能的影响.方法76例仅患有慢-快型房室结折返性心动过速的患者分为两组,老年组(≥60岁)36例和青年组(14~45岁)40例,均为行慢径消融术成功病例,对比消融前后和两组间的房室结功能参数,分析和比较这两组患者在射频消融前后房室结电生理特点的异同.结果所有病例均消融成功.两组病例消融后较消融前房室结前传文氏周期及最长A2H2间期均缩短,而老年组的心动过速周长、消融前后窦性心率周长及消融后房室结前传文氏周期均较青年组延长.结论老年人的房室结前传电生理特性较青年人为差,而对房室结折返性心动过速患者的慢径有效消融后,房室结电生理特性的变化规律不受年龄因素影响.
简介:BackgroundTheprognosticvalueofserumC-reactiveprotein(CRP)inpatientswithinfectiveendocarditis(IE)isnotwellelucidated.ThisstudyaimedtoevaluatetheusefulnessofCRPinpredictingtheoutcomeofIE.MethodsTwohundredninty-sixpatientsfrom2009to2012intheDepartmentofCardiologyatGuangdongGeneralHospitalwerescreenedanddividedintosurgicalandconventionaltreatmentgroups.CRP,whitebloodcell(WBC),erythrocytesedimentationrate(ESR)andotherclinicaldatawereobtainedwithfollow-upfor12months.ResultsTwohundredthirty-sixpatientswereassignedtoreceivesurgerytreatmentwhile60patientsreceivedconventionaltreatment.Inthesurgerygroup,thelevelofCRPinthedeathpatientswassignificantlyhigherthanthatinthesurvivalpatients(P<0.001).TheareaunderthecurveofROCwasabout0.749(SE0.064,P=0.005,95%CI,0.624-0.874)andthecut-offpointofCRPwas23.8mg/L.Inconventionalgroup,therewassignificantdifferencebetweendeathandsurvival(P<0.001).TheareaunderthecurveofROCwasabout0.701(SE0.095,P=0.032,95%CI,0.515-0.888)andthecut-offpointsofCRPwas65.6mg/L.TherewerenosignificantdifferencesinWBCandESRbetweensurgeryandconventionalgroups.ConclusionAmoreaggressivesurgicalinterventionresultsinabetteroutcomeoverconventionaltreatmentandCRPcouldbeservedasapredictivemarkerforadverseoutcomeinIEpatients.
简介:目的评估低密度脂蛋白胆固醇(LDL—C)/高密度脂蛋白胆固醇(HDL—C)比值对经皮冠脉介入(PcI)术后患者心血管事件的预测价值。方法选择急性冠脉综合征(ACS)并予前降支置入支架的患者119例,依据血浆LDL—C/HDL—C比值将患者分为3组,随访1年,评估三组患者心血管事件发生率,以及各危险因素与心血管事件发生率的关系。结果①与LDL—C/HDL—C比值较低的两组相比,比值较高组患者体重指数、女性患者百分率、吸烟人数及糖化血红蛋白、高敏C反应蛋(hs—CRP)、总胆固醇和LDL—C水平均明显升高,而HDL—C水平和他汀类药物使用率则较低(P〈0.05)。②第1组风险(HR)1.04,95%可信区间(c,)0.98-1.08,第2组HR1.16,95%C11.08-1.20,第3组HR1.27,95%C11.19~1.36(P〈0.05)。随着LDL—C/HDL—C比值的升高,PCI术后1年患者心血管事件发生率也逐渐升高(P〈0.05)。③Cox比例风险回归模型提示,LDL—C/HDL—C比值对PCI术后心血管事件风险的预测价值优于其他危险因素。结论LDL—C/HDL—C比值对PCI术后患者1年内心血管事件再发具有一定的预测价值。
简介:ObjectivesTostudythedepressiveeffectoftheantisenseoligonuceotides(ASODN)ofc-mycandproliferatingcellnuclearantigen(PCNA)ontheproliferationofVSMC.MethodsTakingtheVSMCobtainedfromrataortathoracaliscultured4~8generationasresearchobject.Theobjectsweredividedintothreegroupstocarryoutcontrolstudy:controlgroup,PCNAASODNgroupandc-mycASODNgroup.TheASODNs'workingconcentrationallwere1:50.ThedepressiveeffectofASODNonVSMCproliferationwasinvestigatedbycellcounting,MTTand3H-TdRincorporationassay;PCNAandc-mycexpressionweredetectedbyimmunohistochemicalmethodaftertransferringPCNAandc-mycASODNintoVSMC.ResultsPCNAandc-mycASODNcouldinhibittheproliferationofVSMCsignificantly,comparedwithcontrolgroup(P<0.05).②TransferringPCNAandc-mycASODNintoVSMCobtainedsuccessfully;thecorrespondinggenewasinhibitedobviously;comparedwithcontrolgroup(P<0.05).ConclusionsPCNAandc-mycmightplayaconsiderableroleintheVSMCproliferationprocess.ThecorrespondinggenecouldbedepressedsuccessfullyaftertransferringPCNAandc-mycASODNintoVSMC,andthentheproliferationofVSMCwassloweddown.Thisstudypresentedabeneficialproposalandtheoreticalfundamentforatherosclerotictreatment.
简介:Bothendotheliallipasegene(LIPG)584C>T(rs2000813)polymorphismandalcoholconsumptionmodulateserumlipidlevels.Buttheirinteractionsonserumlipidprofilesarenotwellknown.ThepresentstudywasundertakentodetecttheinteractionsofLIPG584C>Tpolymorphismandalcoholconsumptiononserumlipidlevels.GenotypingoftheLIPG584C>Twasperformedin763nondrinkersand520drinkersaged15-85.Interactionsbetweenthegenotypesandalcoholconsumptionwereassessedbyusingacross-productterm.Thelevelsofserumtotalcholesterol(TC),triglyceride(TG),high-densitylipoproteincholesterol(HDL-C),apolipoprotein(Apo)AI,andtheratioofApoAItoApoBwerehigherindrinkersthaninnondrinkers(P<0.01forall).Therewasnosignificantdifferenceinthegenotypicandallelicfrequenciesbetweennondrinkersanddrinkers.ThelevelsofserumTC,HDL-CandApoAIinnondrinkersweredifferentamongthethreegenotypes(P<.05-.01).ThesubjectswithCTgenotypehadhigherserumTC,HDL-CandApoAIlevelsthanthesubjectswithCCgenotype.ThelevelsofserumHDL-CandApoAIindrinkersweredifferentamongthethreegenotypes(P<.001andP<.05;respectively).TheindividualswithTTgenotypehadhigherserumHDL-CandApoAIlevelsthantheindividualswithCCandCTgenotypes.ThelevelsofTCinnondrinkerswerecorrelatedwithLIPG584C>Tallele(P<.05),whereasthelevelsofTGandHDL-CwereassociatedwithLIPG584C>Talleles(P<.05)andgenotypes(P<.05);respectively.ThepresentstudysuggeststhatthesubjectswithTTgenotypebenefitedmorefromalcoholconsumptionthanthesubjectswithCTandTTgenotypesinincreasingserumHDL-CandApoAIlevels.
简介:backgroundBonemarrowmesenchymalstemcells(BMSCs)canbeisolatedandculturedtomanypassages.However,StemcellsincludingBMSCsquicklyundergosenescenceinculture.Thecellsenescenceandmulti-directionaldifferentiationhavehamperedproducingBMSCsinquantitywiththeirundifferentiatedstate.Inthisstudywereportanaturalcompound,vitaminC(Vc),maintainsBMSCsstemproperty.MethodsHumanBMSCswereisolatedfrombonemarrowandpurifiedby1.073g/mLdensitygradientcentrifugation.50ng/mLVcwereaddedtoBMSCsfordifferenttimepoint.FlowcytometrywasusedtodetectcellsurfacemarkersofBMSCswithorwithoutVctreatment.BMSCsproliferationwasanalyzedbyMTTassay.PCR(polymerasechainreaction)andreal-timePCRwereusedfordetectingc-kit,nanog,andOct-4genesexpressionlevels.DNAmethyltransferase(Dnmt)1andDnmt3blevelswerealsodetectedbyreal-timePCR.ResultsFlowcytometryshowedthatafterVctreatmentfor6h,thesurfacemarkersofBMSCswerealmostunchanged.VcincreasedtheproliferationactivityofBMSCsfrom6hto24h.PCRshowedtheexpressionofc-kit,nanog,andoct-4geneswereobviouslyincreasedinVctreatedgroupthancontrolgroupat12h.Real-timePCRshowedthatthelevelofc-kit,nanog,andoct-4geneswereunregulatedfrom6hto12hcomparedwithcontrolgroup.VcalsoincreasedDnmt3bbutnotDnmt1geneexpression.ConclusionsOurresultsshowedVcactsatleastacceleratesBMSCsproliferationandmaintainsstemcellproperty.Inourstudy,wehighlightedamethodofimprovingthespeedofBMSCsgenerationandprovidedadditionalinsightsintothemechanisticbasisofpreventingBMSCssenescence.