简介:摘要:目的 了解沈氏温胆汤的临床使用情况及疗效的体会。方法 通过临床使用情况合多方资料总结沈氏温胆汤的临床应用体会。结果 沈氏温胆汤通过舌定虚实,圆机活法,治疗各种疾病㘬取得良好的作用,结论 进一步证实沈氏温胆汤通过舌定虚实,苔腻温胆汤,苔薄调肾阴阳方,圆机活法在临床中应用意义。
简介:TostudytheexpressioncharacteristicofJapaneseencephalitisvirus(JEV)prMEandEproteinsandtheefficacyofDNAimmunizationbydifferentrecombinantplasmidscontainingJEVprME(2001bp)andE(1500bp)genes,tworecombinants(pJMEandpJE)containingJEVprMEandEgenesfusedwithFLAGwereconstructedandthentransfectedintoHepG2andCOS-1cellsbylipnsomefusion.TheexpressionfeatureofFLAG-prME(about72kDa)andFLAG-E(about54kDa)proteinsintransfectedcellswereanalyzedbyWesternblotandtwoantibodysystems(anti-FLAGandanti-E).BALB/cmicewereimmunizedwith100μgoftwokindsofrecombinantsbyintramuscularinjection,andJEVJaGAr-01strains(10^5PFU/100μl)weregiventoBALB/cmicebyintraperionealinjection3wkaftertwiceDNAimmunizationbyalethalviruschallenge.BALB/cmicewereobservedfor21daysafterchallenge.80%plaquereductionneutralizationtestwasperformedtotitrateneutralizationantibodybeforeandafterviralchallenge.ItwasfoundthattheexpressionofproteinsassociatedwithpJMEandpJEwasdeterminedintransfectedcellswithanti-FLAGandanewproteinof11kDawasdetectedinHepG2andCOS-1cellstransfectedwithpJME.OnlyE(53kDa)proteinwasidentifiedastransfectedwithpJMEusingantiE.HigherlevelofneutralizationantibodiesandtheefficacyofprotectiveimmunitywereinducedwithpJMEimmunization,andweresimilartothoseinducedbyinactivatedJapaneseencephalitisvaccine,butwerebetterthanthoseinducedwithpJE.ItconcludesthattheexpressionlevelfromprMtoEproteinsofJEVisdifferentinvitro,andtheinvitroexpressionefficiencyofpJMEwasbetterthanthatofpiE.FLAG-prMEproteinexpressedbypJMEcouldbecleavedbypeptidasefromhost.TheefficacyofDNAimmunizationiscorrelatedtotheexpressioncharacterizationofrelatedproteinsexpressedinvitro.
简介:【摘要】报告 1例室上速患者使用改良瓦氏动作的急救护理。护理要点包括:快速识别与评估、心理护理、改良瓦氏动作实施、健康教育。经过改良瓦氏动作的实施患者 2 分钟即成功复律。
简介:ToconstructanexpressionvectorcontainingtheE1glycoproteingeneofrubellavirusforthestudyontheeffectofmutationoftheE1geneglycoproteinandtheanalysisofphylogeneticdifferencesofsequences,thegeneencodingtheE1envelopeglycoproteinwasamplifiedfromrubellavirus,JinanstrainJR23,byRT-PCRandligatedintoPMD-18Tvector.TheclonesthatcarriedtheE1genewereidentifiedafteramprselectionandanalysisofrestrictionenzymedigestion.AftersequencingthisgenewasanalyzedbyDanstarandWinstarprograms,andthemapofphylogenetictreewasdrawn.ThecloneofE1glycoproteinwasthusconstructed.ItwasfoundthatthesequencedifferencesbetweenJR23strainandtheTCRBstrainfromJapanandthosebetweenJR23strainandThomasstrainofEnglandwererathersmallwithdifferencevaluesof0.9%and1.2%respectively.YetthosebetweenJR23strainandBRD2strainfromBeijingandthosebetweenJR23strainandXG379strainfromHongKongwerecomparativelylargerwithdifferencevaluesof7.6%and7.3%respectively.ThesequenceofJR23strainwithotherstrainswaslessthan3%excepttheNCstrain(3.7%).ItconcludesthattheconstructionofE1glycoproteingeneoffersanapproachtostudytherelationshipbetweenstructuresandfunctionsofE1geneanditsgeneproducts.Inthephylogenetictree,itshowsthattherearesignificantdifferencesinthesequencesofrubellavirusisolatedinChina,andthismightbehelpfultodevelopaneffectivesubunitvaccine.
简介:【摘要】目的:分析交叉克氏针及微型空心螺钉治疗末节指骨骨折的效果。方法:选择2018年7月-2019年8月接受的54例末节指骨骨折患者为对象,依据治疗差异分组,甲组的27例实施交叉克氏针治疗,乙组的27例实施微型空心螺钉治疗,治疗之后对结果对比。结果:分析和研究两组案例的围手术期指标,乙组的手术时间短于甲组,透析次数少于甲组,乙组的优势明显。分析和对比两组案例的治疗概况,以治疗优良几率作为评价指标,乙组的优良几率高于甲组,分别是92.5%和74.1%,。分析和对比两组案例的并发症情况,乙组的并发症例数2例,甲组是5例,乙组的发生几率低于甲组,分别是7.4%和18.5%。结论:针对末节指骨骨折的患者实施微型空心螺钉治疗,效果明显,优于交叉克氏针治疗效果可行性高,值得实施和借鉴。