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43 个结果
  • 简介:AbstractBrucellosis is an important zoonotic infectious disease and is an important public health problem that causes serious economic consequences to the livestock industry. Brucella spp. comprise one of the most common pathogens causing laboratory-acquired infections (LAIs) and are becoming an increasingly important biosafety issue. To understand the significance of Brucella LAIs in China, related papers were search based on three Chinese databases (CNKI, Wanfang, and VIP), as well as PubMed. After assessment, 37 total cases were evaluated, including 27 students, seven laboratory technicians (one pregnant), two housekeeping staff, and one instructor. The age, sex, incubation period, pathogen detection results, and potential routes of infections were collected and analyzed. All LAIs occurred due to improper operations, inadequate biosafety training, and substandard laboratory safety conditions. Therefore, it is urgent to establish a comprehensive and systematic biosafety prevention/control system in laboratories to protect staff members from accidental exposures and LAIs; further, possible risks and control measures for the management of such infections were proposed.

  • 标签: Brucella Laboratory-acquired infection China
  • 简介:SmallRNA(sRNA)-mediatedpost-transcriptionalregulationdiffersfromprotein-mediatedregulation.Throughbasepairing,sRNAcanregulatethetargetmRNAinacatalyticorstoichiometricmanner.Sometheoreticalmodelswerebuiltforcomparisonoftheprotein-mediatedandsRNA-mediatedmodesinthesteady-statebehaviorsandnoiseproperties.ManyexperimentsdemonstratedthatasinglesRNAcanregulateseveralmRNAs,whichcausescrosstalkbetweenthetargets.Here,wefocusonsomemodelsinwhichtwotargetmRNAsaresilencedbythesamesRNAtodiscusstheircrosstalkfeatures.Additionally,thesequence-functionrelationshipofsRNAanditsroleinthekineticprocessofbase-pairinghavebeenhighlightedinmodelbuilding.

  • 标签: 调控理论 RNA介导 细菌 小RNA 碱基配对 转录后调控
  • 简介:Awidevarietyofdifferenttypesofmicroorganismsareknowntoproduceintracellularenergyandcarbonstorageproducts,whichhavebeengenerallydescribedasbeingpoly(β-hydroxybutyrate),PHB,butwhichare,moreoftenthannot,copolymerscontainingdifferentalkylgroupsattheβ-position.Hence,PHBbelongstothefamilyofpoly(β-hydroxyalkanoastes),PHA,allofwhichareusuallyformedasintracellularinclusionsinbacteriaunderunbalancedgrowthconditions.Recently,itbecameofindustrialinteresttoevaluatethesePHApolyestersasnaturalbiodegradableandbiocompatibleplasticsforawiderangeofpossibleapplications,suchassurgicalsuturesorpackagingcontainers.Forindustrialapplications,thecontrolledincorporationofrepeatingunitswithdifferentchainlengthsintoaseriesofcopolymersisdesirableinordertoproducepolyesterswitharangeofmaterialpropertiesbecausephysicalandchemicalcharacteristicsdependstronglyonthepolymercomposition.Such"tailor-made"copolymerscanbeproducedundercontrolledgrowthconditionsinthat,ifadefinedmixtureofsubstratesforacertaintypeofmicroorganismsissupplied,awelldefinedandreproduciblecopolymerisformed.

  • 标签: COPOLYMER BIODEGRADABLE belongs ALKYL incorporation MONOMER
  • 简介:Severalbacterialstrainswereisolatedfromdifferentrhizospheres.Amongthese,strainPDY7exhibitedstrongantibacterialactivityagainstthericebacterialblight(BB)pathogenXanthomonasoryzaepv.oryzae(Xoo)bythelaboratorydualplateassays.TheantibacterialpropertyofthestrainPDY7wasfurtherinvestigatedfortheproductionof2,4-diacetylphloroglucinol(DAPG),whichamplifiedacharacteristicof629-bpDNAfragmentbyPCR-basedscreeningmethodusingphlDprimers.TheapplicationofphlDpositivestrainswascarefullyevaluatedfordiseasecontrolandgrowthpromotionofriceplantsunderfieldconditions.TheselectedstrainPDY7suppressedthericeBBby58.83%and51.88%underglasshouseandfieldconditions,respectively.Inaddition,thestrainPDY7showedsignificanttwo-foldincreaseinrootlength(18.08cm),shootlength(29.81cm),andgrainyield(96.07g).StrainPDY7promotedthegrowthofriceplantsbyproductionofindole-3-aceticacid(IAA),whichwasdeterminedbyhighperformanceliquidchromatography(HPLC)analysis.OurfindingssuggestthatPDY7belongstotheP.fluorescensgroupandcanserveaspotentialbiocontrolofBBaswellasbiofertilizeragentforgrowthpromotionofrice.

  • 标签: 水稻白叶枯病 白叶枯病菌 根际细菌 生物防治 促生长 高效液相色谱
  • 简介:Polyethyleneimine(PEI)functionalizedFe3O4MNPsweresynthesizedbyamodifiedhypothermaloxidativehydrolysismethod.Themagneticnanoparticlesshowedpositivelychargedsurface,strongmagneticresponsivityanduniformparticlesizedistributionat56.1±0.6nm.Aggregationofthesemagneticnanoparticleswereobservedonthesurfaceofdifferenttypeofbacteria.Magneticcapturingofbacteriawerefacilitatedbythesemagneticnanoparticles.Thecapturingefficiencycouldreach90%aftertworoundsofinteractionsof5minutes.ThemechanismandprocessofinteractionsbetweenbacteriaandpolyethyleneiminefunctionalizedFe3O4magneticnanoparticleswereexploredanddiscussed.ThepresentstudynotonlyprovidesinsightintointeractionsbetweenFe3O4@PEIMNPsandbacterialcells,butalsoopensanewavenuefordesigningandapplyingFe3O4@PEIMNPsasbiosensorsinmicrobiology,medicine,andenvironmentalscience.

  • 标签: MAGNETIC nanoparticles(MNPs) polyethyleneimine(PEI) MAGNETIC separation BACTERIAL
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  • 简介:现在的学习瞄准了估计与海洋的海绵联系的细菌的antifouling活动。八细菌的紧张的一个总数从海绵Sigmadociasp的表面被孤立,他们,SS02,SS05和SS06对形成biofilm细菌显示出禁止的活动。这3紧张的摘录更加影响了生产形成biofilm的能力和粘附的细胞外的聚合物质细菌的紧张。除了圆盘散开试金,microalgal解决试金被执行,摘录与聚氨酯木头光泽混合了并且到不锈钢赠券上涂。紧张SS05的摘录显示出强壮的microalgal解决禁止的活动。紧张SS05基于它的16SrRNA基因作为杆菌仙影拳被识别。与海洋的无脊椎动物联系的细菌的紧张的代谢物答应被发展进环境友好的antifouling代理人。

  • 标签: 海洋细菌 防污剂 海绵 SP 海洋无脊椎动物 抑制活性
  • 简介:以便学习TGF-beta1的结构功能细节,重组体老鼠TGF-beta1的成熟形式在细菌被表示。TGF-beta1(氨基酸279-390)的112氨基酸的carboxyl终端部分的合成被一个可诱导的基因表示系统基于抗菌素T7RNA控制。这个系统允许重组体TGF-beta1的活跃、选择的合成。在减少条件下面在SDS-polyacrylamide胶化上决定的表示TGF-alpha1单体的分子量是大约13kD。连续净化洗与纯化步是足够的净化表示产品到同质的单个胶化过滤结合了。氨基终端的定序表明重组体蛋白质的N终端与出版数据相同。在西方的污点分析,重组体多肽对polyclonalTGF-beta1抗体显示出优秀antigenicity。在这研究表示的成熟重组体老鼠TGF-beta1提供一个有用工具因为未来详细说明了结构、功能的研究。

  • 标签: 转化生长因子-β1 重组表达 包涵体 提纯 抗原性
  • 简介:INTRODUCTIONTheinsolublephosphateswhichcannotbedirectlyabsorbedbyplantsarethemaiformsofinorganicphosphateinsoil.Thesekindsofphosphatescanbesolubilizedbyseveralspeciesofbacteriawhicharewidelyspreadinsoilespeciallyinrhizospherewhere70%ofthebacteriaarecapableofsolubilizinginorganicphosphate.Manyresearchersre-

  • 标签: 红壤 有机酸 水溶性磷肥 无机肥 磷细菌 种类
  • 简介:Afterthesingle-strainabilitiesoforganic-pollutant-degradingbacteriainbioremediationofsedimentenvironmentofshrimpculturearedetermined,themultistraindegradingeffectofthecompositionsofdifferentstrainsismeasured.Theresultsindicatethatthemulti-strainsgroupshavehigherdegradingabilitythanthesingle-straingroups.Three-straingroupsarebetterthantwo-straingroups,andfourstraingroupsarebetterthanthree-straingroupsandfive-straingroups,thegroupscomposedofstrainsLt7222,Lt7511,Fc6308andGy7018hasthebestdegradingeffect,theCODMnremovalrateis73.2%in66h,andgetsto82.7%in114h,30%higherthanthatofthebestsingle-straingroup;GroupsofLt7222,Lt7511,Lt7451andGy7018arethesecond,whoseCODMnremovalrateis82.1%in114h,Itissuggestedthatmulti-speciesbacteriabeusedasfunctionalbacteriainbiorernediationofmaricultureenvironment.

  • 标签: 细菌 污染物 沉积物 环境评价
  • 简介:Thekillinglogarithmsindexinkillingavegetativeforminanexplosureofabout90sandasporeinanexplosureofabout120s,byusingalow-temperatureplasmaproducedbydielectricbarrierdischarge(DBD),reached5.Thespeedinkillingthestrainstested,byusingalow-temperatureplasma,wasthehighestwithE.Coli,thenS.AureusandB.Subtilisvarnigerspore.Theresultsofthescanningelectronmicroscopeshowedthatthelow-temperatureplasmadestroyedtheouterstructureofthebacteriaandthatthevegetativeformwasmoresusceptibletotheinactivationeffectofthelow-temperatureplasmathanwasthespore.Thisindicatedthattheeffectsofthehighvoltageandhighvelocityparticleflow,inplasma,penetratingthroughtheouterstructureofthebacteriamightplayadominantroleduringtheinactivationofthebacteria.

  • 标签: 低温等离子体 细菌 生物失活效应 放电效应 营养体型 孢子
  • 简介:Toexploretheprevalenceoftheplasmid-mediatedquinoloneresistancegeneqnrAinGram-negativebacteriaandtoinvestigateitsmoleculargeneticbackgroundandresistanceprofileinisolatesharboringthisgene,atotalof629nalidixicacid-resistantisolatesofnon-repetitiveGram-negativebac-teriawerecollectedfromclinicalspecimensbetweenApril2004andApril2006andtheseisolateswerescreenedforqnrAgenebyPCRusingspecificprimerscombinedwithDNAsequencing.Theextendedspectrumβ-lactamase(ESBL)orAmpC-producingisolatesweredistinguishedbythephenotypicconfir-matorytestcombinedwithDNAsequencing,andtheantibioticssusceptibilitytestforqnrA-positiveiso-lateswascarriedoutbyKirby-BauerandE-testmethod.TodetectthelocationoftheqnrAgene,plas-midconjugationandSouthernhybridizationwereperformedandtheintegronstructurecontainingtheqnrAgenewasclonedbyPCRstrategyandsequencedbyprimerwalking.ItwasdemonstratedthattheincidenceoftheqnrA-positivestrainsinnalidixicacid-resistantbacteriawas1.9%(12/629),inwhichthedetectionratesforKlebiesiellapneumoniae.Enterobactercloacae,Enterobacteraerogenes,CitrobacterfreundiiandSalmonellachoeraesuiswere2.2%(3/138),17.1%(6/35),9.1%(1/11),12.5%(1/8),and14.3%(1/7),respectively.TheqnrAgenewasfoundtobeembeddedinthecomplexsu/1-typeintegronlocatedonplasmidswithvariedsize(80-180kb).Amongthem,4qnrA-positiveisolatescarriedintegronIn37and8isolatescarriedanovelintegron,temporarilydesig-natedasInX.AlltheqnrA-positiveisolateswereESBL-producingandtransferableforthemuhi-drugresistance.Itisconcludedthattheplasmid-mediateddrug-resistancemechanismexistsinthequinoloneresistantstrainsofisolatesfromhospitalsinGuangdongarea,buttheincidencewasratherlow.Never-theless,itisstillpossiblethatthehorizontaltransferoftheresistantqnrAgenemightleadtothespreadingofdrug-resistance.

  • 标签: 药品抗药性 细菌 序列分析 DNA克隆
  • 简介:在蚂蚁和蚜虫种类之间的Mutualistic相互作用是可观的历史、当代的调查的题目,主要好处正在清洗并且为蚜虫的保护和为蚂蚁的充满糖类的蜜露。然而,问题至于影响这种关系的不稳定的semiochemical因素留下了。最近的研究加亮细菌的蜜露的角色在蚂蚁吸引力的不稳定的混合物。这里,远地区别2蚜虫种类的蚂蚁能力用双向olfactometer基于细菌的蜜露semiochemicals排出物被调查。两mutualistic蚜虫蚜虫属fabaeL。并且nonmyrmecophilous蚜虫Acyrthosiphonpisum哈里斯被发现为蚂蚁Lasius尼日尔漂亮L。吸引力的水平在两试金是类似的(控制对蚜虫种类之一)。不管多么当给在这之间的一种选择时2蚜虫种类,蚂蚁为蚜虫属fabae显示出重要偏爱。主要从细菌的起源,蜜露volatiles被知道是在蚂蚁吸引力的一个关键元素。用一样的olfactometry协议,蜜露射出的volatiles的相对优美从每蚜虫种收集了,由从各个孤立的细菌,蜜露被调查。再次,蚂蚁显著地比较喜欢蚜虫属fabae蜜露和细菌释放的volatiles。这个信息建议微生物引起的蜜露volatiles使蚂蚁能远地区别蚜虫种类。这些结果加强在昆虫symbioses学习出现和微生物的潜在的影响的兴趣。

  • 标签: 共生关系 蚜虫 蚂蚁 细菌 挥发性化合物 碳水化合物
  • 简介:AbstractColistin is an old antimicrobial that has been revitalized as last-resort treatment against multidrug-resistant (MDR) gram-negative bacterial infections. However, colistin has been widely used in agricultural production and veterinary medicine for decades, and the recent global dissemination of mobilized colistin resistance (mcr) genes from animals to humans seriously threats the clinical use of colistin. Most of the mcr-harboring isolates have been Enterobacteriaceae, such as Escherichia coli and Salmonella enterica which are common to animals and humans. An understanding of the origin, dissemination and transmission of mcr genes in bacteria common to animals and humans will facilitate the management of colistin use and relevant interventions to prevent further spread of resistance. This review aims to provide a comprehensive assessment of the global prevalence and transmission of mcr genes of animal and human commensal/pathogenic bacteria.

  • 标签: Colistin Antibiotics resistance mcr genes Transmission Animals and humans
  • 简介:Tosupportthescientificbasisforrapididentificationofpathogenicbacteriaandotherstud-ies,thesequencesofhsp60geneinmajor34speciesof16genusofpathogenicbacteriaweresearchoutinGenBankandaproperpairofuniversaldegenerateprimerwasdesignedbymeansofthemolecu-larbiologicalsoftwawePrimer5.0andOligo6.0.ThisprimerwasthenusedinthePCRamplification,andthehsp60genefragmentsoftheselectedpathogenicbacteriacouldbeamplifiedusingthisdegener-ateprimer.Bywayofbioinformationalanalysis,theconservation,variationandtheinterspeciesphylo-geneticrelationsofthehsp60genesequencewereanalysed.Fromtheresultsofthecomparativestudyonsequences,itwasdemonstratedthatthehsp60genewascharacterizedbyconservationandvaria-tion,inwhichtheconservedandmutantregionsco-existedandseparatelydistributedwithmanysmallmutantregionsdistributedamongtheconservedregions,justlikethemosaic.Thephylogenetictreeamongdifferentpathogenicbacteriadrawnfromthehsp60geneanalysiswasprovedtobeconsistentwiththosefrom16SrRNAand23SrRNA.Itisconcludedthatthesequencedistributionofhsp60genewouldprovideasolidbasisfortherapididentificationofpathogenicbacteriaandthedevelopmentofadiagnosticmicroarray.

  • 标签: 种系发生 致病基因 遗传基因 层次分析
  • 简介:AgreatamountoffoodbornepathogenswereGram-positive(G?)bacteria,athreattopublichealth.Inthisstudy,consideringthebindingabilityofnisintowardsG-bacteriaandthestablefluorescentabilityofEGFPprotein,afluorescentnisin–EGFPproteinprobewasconstructedbyageneengineeringmethod.NisinandEGFPwereusedasthereceptorandfluorophore,respectively,todetectG?bacteria.ThenisinandegfpgenewereamplifiedseparatelyaccordingtothesequencepublishedinGenBankusinguniqueprimers.ThetwogeneswereclonedintoapET-28b(?)vectorresultinginapET-28b(?)–nisin–egfpvector.ThevectorwastransferredintoEscherichiacoli(E.coli)BL21(DE3)forexpression.Theexpressedproteinwasextracted,purifiedbyaNi–NTAcolumn,andthentestedbytheSDS-PAGEmethodtoconfirmitsmolecularweight.Listeriamonocytogenes(L.monocytogenes),Staphylococcusaureus(S.aureus),andMicrococcusluteus(M.luteus)wereusedastherepresentationsofG?bacteria.E.coliO157,representingthegram-negative(G-)bacteria,wasusedasanegativecontrol.Thebindingspecificityoftherecombinantproteinwasperformedontwotypesofbacteriaandthendetectedthroughfluorescentmicroscopy.Theresultsindicatedthatthenisin–EGFPprobecoulddetectG?bacteriaat108CFU/mL.

  • 标签: Nisin–EGFP GRAM-POSITIVE bacteria Fluorescent DETECTION PROBE