Differentialproteomeprofilesofhumanlungsquamouscarcinomatissuecomparedtopairedtumor-adjacentnormalbronchialepithelialtissuewereestablishedandanalyzedbymeansofimmobilizedpHgradient-basedtwo-dimensionalpolyacrylamidegelelectrophoresis(2-DPAGE)andmatrix-assistedlaserdesorption/ionizationtimeofflightmassspectrometry(MALDI-TOF-MS).Theresultsshowedthatwell-resolved,reproducible2-DEpatternsofhumanlungsquamouscarcinomaandadjacentnormalbronchialepithelialtissueswereobtainedundertheconditionof0.75-ugprotein-load.Theaveragedeviationofspotpositionwas0.733+0.101mminIEFdirection,and0.925+0.207mminSDS-PAGEdirection.Fortumortissue,atotalof1241±88spotsweredetected,987±65spotswerematchedwithanaveragematchingrateof79.5%.Forcontrol,atotalof1190+72spotsweredetected,and875±48spotswerematchedwithanaveragematchingrateof73.5%.Atotalof864±34spotswerematchedbetweentumorsandcontrols.Forty-threedifferentialproteinswerecharacterized:someproteinswererelatedtooncogenes,andothersinvolvedintheregulationofcellcycleandsignaltransduction.Itissuggestedthatthedifferentialproteomicapproachisvaluableformassidentificationofdifferentiallyexpressedproteinsinvolvedinlungcarcinogenesis.Thesedatawillbeusedtoestablishhumanlungcancerproteomedatabasetofurtherstudyhumanlungsquamouscarcinoma.
