HPLC and Pattern Recognition for Identification of Four Species of Hongmu

(整期优先)网络出版时间:2012-03-13
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FurnitureofHongmu,creamofChineseculture,hasalonghistoryandenjoysgreatpopularityforitsclassicappearanceandelegantdisposition.Thetraditionalmethodoftheidentificationistoevaluatethemacroformandanatomicalfeatures.Inthispaper,anewmethodbasedonthedifferencesamongthechemicalcharacteristicsofbiologicalmetabolitesofsamplesisdeveloped.Intheresearch,highperformanceliquidchromatography(HPLC)fingerprintswereemployedtodiscriminatefourspeciesofHongmu,includingPterocarpusindicus,Pterocarpusmacarocarpus,Dalbergiamelanoxylon,andDalbergialouvelii.Theresultsshowedthat,accordingtotheretentiontimeandrelativepeakareaofdifferentcomponentsinwoodextracts,thedistinctioncanbeeasilymadebetweendifferentspecies.BasedontheHPLCfingerprints,similaritiesbetweensampleswereevaluatedbycorrelationcoefficients.Theresultsweregreaterthan0.81betweenthesamespecies,andlessthan0.21betweendifferentspecies,whichshowedweakcorrelationbetweendifferentspecies.Inordertoestimatetheoverallfeaturesofthefingerprints,hierarchicalclusteringandprincipalcomponentanalysis(PCA)wereusedandtheresultsshowedpronouncedclusteringeffectamongthesamespecies.TheinvestigationindicatedthatpatternrecognitioncouldaccuratelyreflectthesignificantdifferencesbetweendifferentspeciesofHongmu,andcanbeusedasareferenceforqualitycontrol.