简介：目的探讨康柏西普联合激光光凝治疗视网膜分支静脉阻塞继发黄斑水肿的效果。方法选择本院2016年1月至2017年6月收治的90例（98眼）视网膜分支静脉阻塞继发黄斑水肿患者,根据随机数字表法将其分为两组,各45例（49眼）。对照组采用激光光凝治疗,观察组采用康柏西普联合激光光凝治疗,比较两组最佳矫正视力、黄斑中心视网膜厚度。结果观察组治疗1周后、治疗3个月后BCVA水平较对照组高,CMI水平均较对照组低,差异具有统计学意义（P〈0.05）。结论康柏西普联合激光光凝治疗视网膜分支静脉阻塞继发黄斑水肿效果确切,可明显改善黄斑中心视网膜厚度,促进患者视力恢复。

简介：目的：探讨老年翼状胬肉切除与自体角膜缘干细胞移植术后硬性角膜接触镜的干预效果。方法：选取2015-03/2016-03经我院诊断为单侧翼胬肉老年患者90例90眼进行分组对比研究。观察组45例45眼采用自体角膜缘干细跑移植术后硬性角膜接触镜,对照组45例45眼行单纯自体角膜缘干细胞移植术。两组患者观察指标包括：角膜愈合时间和术后1、3、5、7d时两组患者疼痛程度（VAS评分）、胬肉复发几率。结果：观察组和对照组的角膜刺激症状评分存在统计学差异,观察组评分显著低于对照组患者（P〈0.05）。对照组患者角膜愈合时间高于观察组患者,差异具有统计学意义（P〈0.05）;VAS评分显示,术后1、2d时对照组患者高于观察组,两组比较差异具有统计学意义（P〈0.05）;两组患者胬肉愈合复发几率对比,差异无统计学意义（P〉0.05）。结论：自体角膜干细胞移植术后结合硬性角膜接触镜对老年翼状胬肉效果明显,患者愈合较快,还可减轻患者术后疼痛感,同时不会增加胬肉复发率。

简介：AIM:Todeterminethehistopathologicalchangesofrifampicinappliedintravitreallyonretinalganglioncellsbymeansofstereologicalandhistopathologicalmethods.METHODS:Forthisstudytwenty-fourNewZealandadultrabbitsweredividedintofourgroups(n=6foreachgroup).50μg/0.1mL(group1),100μg/0.1mL(group2),150μg/0.1mL(group3)and200μg/0.1mL(group4),rifampicinwereinjectedintothevitreousoftherighteyesofanimals,theirlefteyeswereusedascontrol(group5).Afterthe28thdayofapplication,animalswereanesthetisedwithxylazine(8mg/kg,IM)andthentheireyeswereenucleatedimmediately.Patternsweretakenawayandeyeswerepreparedforbothstereologicalandelectromicroscopicobservation.RESULTS:Dependingonthehighdoseofrifampicin,somehistopathologicalchangessuchascytoplasmicdilatationanddamagedmembranewereobservedontheelectromicroscopiclevel.Usingquantitativeexamination,whichwasdoneatthelightmicroscopiclevel,itwasshownthatthenumberofneuronsdecreasedlinearlyasrifampicindoseincreasedwhencomparedwiththecontrolgroup.CONCLUSION:Basedonthesefindings,low-doserifampicin(50μg/0.1mL)maybeusefulfortreatmentoftheoculardiseases.

简介：AIM:Toevaluatetheeffectofbrinzolamide-timololfixedcombinationonintraocularpressure(IOP)aftercataractsurgery.METHODS:Thestudyincluded92eyesof87patientswhounderwentcataractsurgeryandintraocularlensimplantation.Patientsscheduledforphacoemulsificationwereassignedto1of2groups.Thetreatmentgroupreceived1dropofbrinzolamide-timololfixedcombinationimmediatelyaftersurgery,andthecontrolgroupreceivednotreatment.TheIOPwasmeasuredpreoperativelyandat2hand24hpostoperatively.RESULTS:ThemeanIOPchangewaslowerinthetreatmentgroupthaninthecontrolgroupat2hpostoperatively.ThedifferencebetweenthemeanIOPvaluesofthetwogroupsat2hpostoperativelywasfoundtobestatisticallysignificant.Twenty-fourhoursafterthesurgery,themeanIOPchangewasstillhigherinthecontrolgroupwhencomparedtothetreatmentgroup.CONCLUSION:ThefixedcombinationbrinzolamidetimololcaneffectivelyreduceIOPaftercataractsurgery.

简介：AIM:ToinvestigatetheeffectofintravitrealinjectionofDL-alpha-aminoadipicacid(DL-α-AAA)onocularrefractivestateandretinaldopamine,transforminggrowthfactor-β2(TGFβ2),vasoactiveintestinalpolypeptide(VIP)inguineapigform-deprivedmyopia.METHODS:Four-week-oldpigmentedguineapigswererandomlyassignedto4groups:normalcontrol,deprivation,deprivationplusDL-α-AAA,deprivationplussaline.Formdeprivationwasinducedwiththeself-madetranslucenteyeshields,andlastedfor14days.8μgDL-α-AAAwasinjectedintothevitreouschamberofdeprivedeyes.Thecornealradiusofcurvature,refractionandaxiallengthweremeasured.Retinaldopaminecontentwasevaluatedbythehigh-performanceliquidchromatographywithelectrochemicaldetection,andTGFβ2andVIPproteinweredetectedbyWesternblotting.RESULTS:Fourteendaysofeyeocclusioncausedtheaxiallengthtoelongateandbecomemyopicintheform-deprivedeyes,withthedecreaseofretinaldopamineandtheincreaseofTGFβ2andvasoactiveintestinalpolypeptide(VIP)protein.IntravitrealinjectionofDL-α-AAAcouldinhibitthemyopicshiftfrom(-3.65±1.06)Dto(-1.48±0.63)D,P<0.01duetogogglesoccludingandcausethedecreaseofretinalTGFβ2proteininthedeprivedeyes.However,intravitrealinjectionofDL-α-AAAhadnosignificanteffectonretinaldopamineandVIPproteinindeprivedeyes.RetinalTGFβ2proteincorrelatedhighlywiththeocularrefraction(y=-3.34+0.31/x,F=74.75,P<0.001)andaxiallength(y=8.39-0.02/x,F=48.32,P<0.001)indifferenttreatmentgroups.·CONCLUSION:IntravitrealinjectionofDL-α-AAAiseffectivelyabletosuppressthedevelopmentofformdeprivationmyopia,whichmaybeassociatedwithretinalTGFβ2proteininguineapigs.

简介：AIM:Toassesstheeffectivenessofimmunosuppressantsintheprophylaxisofcornealallograftrejectionafterhigh-riskkeratoplastyandnormal-riskkeratoplasty.METHODS:WesearchedtheCochraneCentralRegisterofControlledTrials(CENTRAL),MEDLINE,EMBASE,CNKI,VIPandreferencelistsofarticles.Dateofmostrecentsearch:18June,2011.Allrandomisedcontrolledtrials(RCTs)assessingtheuseofimmunosupressantsinthepreventionofgraftrejection,irrespectiveofpublicationlanguage.Twoauthorsassessedtrialqualityandextracteddataindependently.Onlydichotomousoutcomes(cleargraftsurvival,ratioofimmunereactionsandsideeffects)wereavailableandwereexpressedasrelativerisk(RR)and95%confidenceintervals(CI).RESULTS:Sevenstudieswereincludedinthisreview.Inthecomparingofmycophenolatemofetil(MMF)withplacebo,theresultsshowedMMFcouldsignificantlyreduceimmunereactionscomparedwithplacebo(RR1.0895%Cl0.95to1.21),butnoeffectoncleargraftsurvival(RR1.1195%Cl0.90to1.35).Incleargraftsurvivalandimmunereactions,MMFandcyclosporineA(CsA)showedsimilareffect(RR1.1195%Cl0.90to1.35,andRR1.48,95%Cl0.56to3.93,respectively).Tacrolimus(FK506)andsteroidshowedsimilareffectsoncleargraftsurvivalandimmunereactions(RR0.32,95%CI0.02to6.21,andRR1.00,95%CI0.88to1.14,respectively).Nodrugrelativesideeffecthasbeenfound.CONCLUSION:MMFmayreduceimmunereactionsinbothnormal-riskandhigh-riskrejectionofpenetratingkeratoplasty.CsAandFK506showedsimilareffectsasMMF.However,duetothelackoflargeclinicaltrials,theevidenceremainweak,thequalityofevidenceswereratedasverylowtomoderate.Large,properlyrandomised,placebo-controlled,doublemaskedtrialsareneededtoevaluatetheeffectofimmunosuppressants.

简介：AIM:Tocomparetheeffectoftopicallyadministeredandsubconjunctivallyinjectedbevacizumabonexperimentalcornealneovascularizationinratsfortwoweeksaftertreatment.METHODS:Twenty-eightSprague-Dawleyratsweredividedintofourgroupsof7animals.Eachcornealcenterofrighteyewascauterizedwithsilver/potassiumnitratefor8s.Aftercornealburning,bevacizumab(12.5mg/mL)wastopicallyadministeredthreetimesperday(TBgroup)fortwoweeksorsubconjunctivallyinjectedondays2and4aftercauterization(0.02mL;SBgroup).Asnegativecontrols,ratsreceived0.9%salinetopicallythreetimesperday(TSgroup)orsubconjunctivallyondays2and4(0.02mL;SSgroup).Digitalphotographsofthecorneaweretaken1and2weeksaftertreatmentandanalyzedtodeterminetheareaofcorneacoveredbyneovascularizationasthepercentageofcornealneovascularization.RESULTS:Oneweekaftertreatment,thepercentageofcornealneovascularizationwassignificantlylowerintheTBandSBgroupsthanintheTSandSSgroups(allP<0.05).Twoweeksaftertreatment,thepercentageofcornealneovascularizationwassignificantlylowerintheTBgroupthanintheTSgroup(P<0.05).Inallgroups,thepercentageofneovascularizationwasdecreasingastimepassed(allP<0.05)CONCLUSION:Topicallyadministeredbevacizumabhaslongerstandinganti-angiogeniceffectthansubconjunctivallyinjectedbevacizumabincornealneovascularizationfollowingchemicalinjuryinrats.

简介：目的：分析抗血管内皮生长因子（VEGF）药物加Ahmed青光眼引流阀（AGV）对新生血管性青光眼（NVG）的疗效和安全性。方法：选取2020.01-2020.11本院收治的NVG患者10例为研究对象，均通过抗VEGF药物加AGV开展治疗，观察术后不同时间的手术成功率；术前及术后不同时间的眼压水平、视力改变、视功能受损眼病患者生活质量评定量表（QOL）评分和并发症情况。结果：术后1d及1周，手术成功率达到100.00％，后伴随时间延长有轻微下降，但术后不同时间的手术成功率相比无显著差异（P＞0.05）。术后1d及1周，眼压水平较术前显著下降（P＜0.05），至术后1个月眼压有轻度升高，但仍低于术前（P＜0.05），术前及术后眼压水平相比差异显著（P＜0.05）。术后1d及1周，视力提升比例达到100.00％，术后1个月开始有轻微下降，但术后不同时间的视力水平相比无显著差异（P＞0.05）。术后，患者QOL评分术前显著升高（P＜0.05），至术后1个月QOL评分有轻度下降，但仍高于术前（P＜0.05）。10例患者中1例出现前房少量出血，1例出现轻度角膜水肿，均未经特殊处理后自行消失，无1例出现严重并发症。结论：抗VEGF加AGV对NVG患者疗效确切，能改善其视力，降低其眼压水平，且安全性较高，值得采用。

简介：目的探讨前列腺素类药结合玻璃体腔注射雷珠单抗治疗新生血管性青光眼的效果。方法伴发高眼压的新生血管性青光眼患者160例根据随机数字表法分为治疗组80例与对照组80例,两组都给予复合式小梁切除术,对照组选择拉坦前列腺素辅助治疗,治疗组选择拉坦前列腺素联合玻璃体腔注射雷珠单抗辅助治疗。结果治疗后治疗组与对照组的治疗有效率分别为97.5%和88.8%,治疗组的治疗有效率明显高于对照组（P〈0.05）。两组治疗后最佳矫正视力都明显提高,而眼压都明显下降,与治疗前对比差异明显（P〈0.05）;同时治疗后治疗组的最佳矫正视力与眼压也都明显好于对照组（P〈0.05）。治疗期间治疗组的眼结膜充血、前房炎症反应、角膜水肿、一过性视觉模糊等并发症发生率都明显低于对照组（P〈0.05）。所有患者治疗后随访调查6个月,治疗组的治疗后3个月与6个月的复发率分别为1.3%和5.0%,而对照组分别为7.5%和13.8%,治疗组治疗后3个月与6个月的复发率明显少于对照组（P〈0.05）。结论前列腺素类药结合玻璃体腔注射雷珠单抗治疗新生血管性青光眼能促进眼压的降低,改善视力水平,安全性好,降低复发,从而有利于近远期疗效的提高。

简介：随着互联网技术的发展及其与教育的有机结合，眼科教学面临新的机遇和挑战。应完善基于医疗“大数据”技术、虚拟现实技术和移动互联技术为主要内容的眼科教学方法，提升“互联网＋”背景下眼科教师的教学素养，从而促进学生自主学习、个性化学习，达到提高学习效率、改善学习效果的目的。

简介：目的研制一种实用型显微手术教学培训眼球固定台.方法利用有机玻璃、注射器、压力表等制作一种用于显微手术教学培训的眼球固定台.结果使用方便、灵活,压力稳定性强,有利于清洁,取材方便,制作简单,成本低廉.结论该固定台是一种值得推广应用的显微手术教学与培训的眼球固定台.

简介：上课是常规教学中一个很重要的环节,课堂教学的效率高低直接影响到教学质量的好坏。多媒体课件质量、多媒体与传统板书教学的融合以及多媒体教学方式好坏直接决定教学质量,笔者在对这一问题探讨和分析后,试图寻找出一套较为完整的适合于该课程的教学方式,提高学生学习兴趣,使学生掌握教学重点和难点,取得更好效果。

简介：在瞬息万变的信息化社会里,加强医学生创新能力的培养是适应医学发展的必要条件。本文结合眼科学的特点,初步探讨在眼科教学活动中医学生创新能力培养所存在的问题和解决方案,为提高医学生的创新能力,更好的服务临床提供帮助。

简介：目的分析初发翼状胬肉术后患者应用于退翳明目汤治疗后的疗效。方法采用随机数字表法将我院例初103发翼状胬肉术后患者分组,对照组51例应用典必殊滴眼液治疗,观察组52例给予退翳明目汤治疗,对比两组患者治疗后临床疗效、泪膜稳定性及术后复发率。结果观察组水肿消退、角膜创面上皮修复时间及干眼症状计分均低于对照组,SchirmerBUT水平均高于对照组,复发率0%低于对照组13.89P〈0.05）-Ⅰ及%（。结论退翳明目汤能够有效提升初发翼状胬肉术后患者泪液分泌量,提升泪膜稳定性,消除干眼症状,促进创面上皮修复,降低复发率。

简介：AIM:ToinvestigatetheexpressionsoftypeIcollagen,α2integrinandβ1integrinintheposteriorscleraofguineapigswithdefocusmyopiaandwhetherbasicfibroblastgrowthfactor(bFGF)injectioninhibitstheformationanddevelopmentofmyopiabyupregulatingtheexpressionoftypeIcollagen,α2integrinandβ1integrin.METHODS:After14daysoftreatment,therefractivestateandaxiallengthweremeasuredandthelevelsoftypeIcollagen,α2integrinandβ1integrinwereassayedintheposteriorscleraeofgroupsofguineapigsthatworeamonocular-7Dpolymethylmethacrylate(PMMA)lensorhad-7DlenswearfollowedbytheperibulbarinjectionofPhosphateBufferSolution(PBS)orbFGF.Theuntreatedfelloweyeservedasacontrol.Guineapigswithnotreatmentservedasnormalgroup.·RESULTS:Theresultsshowedthat14daysofmonoculardefocusincreasedaxialeyelengthandrefraction,whilebFGFdeliveryinhibitedthemmarkedly.Further,itwasalsofoundthatthemonocular-7DlenscoulddecreasethelevelsoftypeIcollagen,α2integrinandβ1integrinexpressions,while,unlikePBS,bFGFincreasedthemsignificantlyincomparisontocontralateralcontroleyesandnormaleyes.CONCLUSION:bFGFcanpreventtheformationanddevelopmentofdefocusmyopiabyupregulatingtheexpressionsoftypeIcollagen,α2integrinandβ1integrin.Takentogether,ourresultsdemonstratethatbFGFpromotesscleraremodelingtopreventmyopiainguineapigs.

简介：AIM:ToInvestigatetheeffectsoftransforminggrowthfactorβ2(TGF-β2)andconnectivetissuegrowthfactor(CTGF)ontransdifferentiationofhumanlensepithelialcells(HLECs)culturedinvitroandsynthesisofextracellularmatrix(ECM).METHODS:HLECsweretreatedwithTGF-β2(0,0.5,1.0,5,10μg/L)andCTGF(0,15,30,60,100μg/L)fordifferenttimes(0,24,48,72h)invitroandtheexpressionofα-smoothmuscleactin(α-SMA),themaincomponentoftheextracellularmatrixtypeⅠcollagen(Col-1)andfibronectin(Fn)weremeasuredbyusingreal-timepolymerasechainreaction(PCR)andwestern-blot.RESULTS:TGF-β2andCTGFsignificantlyincreasedexpressionofα-SMAmRNAandprotein(P<0.05,P<0.001),FnmRNAandprotein(P<0.001),Col-1mRNAandprotein(P<0.001).TGF-β2couldinduceHLECsexpressionofCTGFmRNAandproteinindosedependentmanner(P<0.05,P<0.001).TGF-β2andCTGFcouldinduceHLECstoexpressα-SMA,FnandCol-1intime-dependentmanner.EachtimeofTGF-β2andCTGFinducedHELCsexpressionofα-SMA,Fn,Col-1mRNAandproteinwassignificantincreasecomparedwithcontrol(P<0.05,P<0.001).CONCLUSION:TGF-β2andCTGFcouldinduceHLECsepithelialmesenchymaltransitionandECMsynthesis.