简介：ThispaperdealswiththetemperaturecorrelationofgrayscaleofBmodeultrasoundimagefromheatedtissue.Inthisstudy,manyin-vitrofreshpigliversareheatedinatemperaturerangefrom28℃to45℃,fromwhichaseriesofB-modeultrasonicimagesofliverswereobtained.Thegray-valueisevaluatedfromtheultrasoundimagesrespectively.Acorrelationofthemeangrayvalueoftheselectedregions(12×12pixels)inB-modeultrasonicimagesofliveranditstemperaturewaspointedout.Andtheexperimentresultsagreedtheevaluationwell.Anditispossibletomonitorthetissuetemperaturechanginginhyperthermiausingthiscorrelation.

简介：为软织物重建得到一种新支架材料，我们通过混合crosslinked准备了XLHA-PNIPAAm和XLHA-MC血管注射剂hydrogels哈(XLHA)并且二温度敏感的材料poly在降级不同(N-isopropylacrylamide)(PNIPAAm)并且methylcellulose(MC)分别地。我们测试了injectablility，酶的biodegradability，温度敏感，结构cytotoxicity和二血管注射剂hydrogels的溶血。我们的研究成功地获得了XLHA-PNIPAAm血管注射剂hydrogel的准备条件，并且验证了那补充说非可能减解的材料PNIPAAm能推迟降级哈更有效地比可能减解的材料MC。与5kGy剂量放射准备的PNIPAAm，MBAAm/NIPAAm(M/M)=0.015，单体concentration=3%与最慢酶的biodegradability生产了XLHA-PNIPAAm。DSC结果证明XLHA-PNIPAAm的温度敏感比XLHA-MC的更稳定。二合成hydrogels在cytotoxicity和溶血测试被限制，XLHA-PNIPAAmhydrogel的biocompatibility比XLHA-MChydrogel更好出现了。

简介：Thispaperdevelopesatemperaturecontrolsysteminordertocarryoutarealtimeandcontinuousobservationonlivingcellsunderthemicroscope.Inthissystem,transparentindiumtinoxid(ITO)conductivefilmwasusedasaheater,temperaturewasmeasuredbythetemperaturedependenceofresistancebehavioroftheITOfilm,C8051F340single-clupwasusedasthecontrolhardwarecore,andthemodifiedPIDalgorithmandpulsewidthmodulation(PWM)wereadoptedasthekeycontrolsoftwareprograms.Thetemperaturesystemhadasimplestructurewithoutaddedtemperaturesensor,anuniformtemperaturedistributionwithin±1℃ontheITOfilmsurfaceandtemperaturecontrolprecisionof±0.2℃couldbeobtained.ThesystemcouldmaintainagoodgrowthstateforBHK-21livingcellunderthemicroscopefor48h.

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简介：Topretreatspermatvarioustemperaturesbeforeexposuretohumanpapillomavirus(HPV)16DNAfragmentsandtoassesstheefficiencyofHPVcarrierspermtotransfectcumuluscells.Methods:Cumuluscellsfromfollicularaspirateswereobtained,pooledanddividedintoculturedishescontainingSybyGold-stainedHPVDNAcarryingspernthatwereeitherpretreatedat4℃,37℃or40℃(n=5).Thecellswereincubatedin5%Co2inairmixtureat37℃for24hours.TheeffeiciencyofspermtotakeupfluorescentHPVDNAwasdeterminedathour0.Afterincubation.cumuluscellviabilitywasassessedusingtheeosinmethodandthepercentagesoffluorescentcumuluscellsdetermined.Results:Ouverhalfofallthecumuluscellsbecamefluorescentwiththehighestpercentageinthe37℃group.Spermpretreatedat4℃hadthegreatestamountofHPVDNAfragments.Totalspernmotilitywassimilarforthe3pretreatmentgroups.Therewerenodifferencesincumulusviabilityamongthegroups.Conclusion:Spermpretreatedat37℃transferredthegreatestamountoffluorescentHPVDNAfragmentstothecumuluscells.TheHPVDNAwasobservedinthenuclearandcytoplasmiccompartments.ThedatasuggestedthepossibilityofspermasavectorforthetransmissionofHPVDNAtothecumuluscellssurroundingovulatedoocytes,whichmightleadtoearlyimplantationfailures.

简介：Theroomtemperaturecuringdenturebaseresinhaslowmechanicalproperties,soitwaslimitedinclinicalapplication.Ithadpreviouslydisscusedthatreinforedmaehinicalpropertieswithmetalfiber,glassfiber,plasticfiberandbyaddingthefractureresistancesubstancesinpowderTheaimofthisstudywastodeterminedthemechanicalproperitiesoffourroomtemperaturecuringdenturebaseresinwhichhadbeenmodifiedperformancewithhighboilpointmethacrylate.

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简介：Objective:　Toexploretheregulationofearlybraintissuemetabolicchangingafterbraininjuriesandtheclinicalsignificance.　　Methods:　Therewere17patientswithbraininjuries.EarlydirectmonitoringofPtiO2,PtiCO2,pHandbraintemperature,dynamicobservationoftherelationbetweenvariousparametersandclinicsafterbraininjurieswereperformed.　　Results:　EarlychangesofPtiO2,PtiCO2andpHwerecloselycorrelatedwithoutcome.ThedeathrateobviouslyincreasedwhenPtiO2wascontinuouslylowerthan9mm?Hgwithin24hoursafterinjuries.Secondarybraininjuryprolongedandaggravatedbraintissuemetabolicdisturbance.Whenintracerebralpressurewasover30mm?HgPtiO2begantodecrease.Thebraintemperatureinbraindeathpatientswasevidentlylowerthanaxillarytemperature.　　Conclusions:　ThedirectmonitoringofPtiO2,PtiCO2,pHandbraintemperatureissafeandaccurateandcanfindearlyanoxiadamagetobraintissueandprovidereliablebasisforclinicaltherapy.Ithasaninstructivesignificanceinselectingandstudyinganewtreatmentmethodinbraininjuries.Anditcanbetakenasacriterioninclinicaljudgingbraindeaths.

简介：Objective:Tostudythechangesofpartialpressureofbraintissueoxygen(PbtO2)andbraintemperatureinacutephaseofsevereheadinjuryduringmildhypothermiatherapyandtheclinicalsignificance.Methods:Onehundredandsixteenpatientswithsevereheadinjurywereselectedanddividedintoamildhypothermiagroup(n=58),andacontrolgroup(n=58)accordingtooddandevennumbersofhospitalization.WhilemildhypothermiatherapywasperformedPbtO2andbraintemperatureweremonitoredfor1-7days(mean=86hours),simultaneously,theintracranialpressure,rectumtemperature,cerebralperfusionpressure,PaO2andPaCO2werealsomonitored.Thepatientswerefollowedupfor6monthsandtheprognosiswasevaluatedwithGOS(Glasgowoutcomescale).Results:ThemeanvalueofPbtO2within24hourmonitoringinthe116patientswas13.7mmHg±4.94mmHg,lowerthanthenormalvalue(16mmHg±40mmHg)ThetimeofPbtO2recoveringtothenormalvalueinthemildhypothermiagroupwasshortenedby10±4.15hourscomparedwiththecontrolgroup(P<0.05).Thesurvivalrateofthemildhypothermiagroupwas60.43%,higherthanthatofthecontrolgroup(46.55%).Aftertherecoveryofthebraintemperature,PbtO2increasedwiththeriseofthebraintemperature.Conclusions:Mildhypothermiacanimprovethesurvivalrateofsevereheadinjury.ThetechniqueofmonitoringPbtO2andthebraintemperatureissafeandreliable,andhasimportantclinicalsignificanceinjudgingdiseaseconditionandinstructingclinicaltherapy.

简介：AbstractBackground:Hypothermia is associated with many adverse clinical outcomes in pediatric patients, and thus, it is important to find an effective and safe method for preventing peri-operative hypothermia and its associated adverse outcomes in pediatric patients. This study aimed to investigate the effect of forced-air warming blankets with different temperatures on changes in the transforming growth factor-β (TGF-β), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-10 levels in children undergoing surgical treatment for developmental displacement of the hip (DDH).Methods:The study included 123 children undergoing surgery for DDH under general anesthesia. The patients were randomly assigned to three groups, using a random number table: the 32, 38, and 43°C groups according to the temperature setting of the forced-air warming blankets. For each patient, body temperature was recorded immediately after anesthesia induction and intubation (T0), at initial incision (T1), at 1 h after incision (T2), at 2 h after incision (T3), at the end of surgery (T4), immediately upon return to the ward after surgery (T5), and then at 12 h (T6), 24 h (T7), 36 h (T8), and 48 h (T9) after the surgery. The serum levels of TGF-β, TNF-α, IL-1β, and IL-10 were measured at T0 and T4 for all groups.Results:The number of patients with fever in the 38°C group was significantly less than those in the 32 and 43°C groups (χ2 = 6.630, P= 0.036). At T0, the body temperatures in the 38 and 43°C groups were significantly higher than that in the 32°C group (F = 17.992, P < 0.001). At T2, the body temperature was significantly higher in the 43°C group than those in the 32 and 38°C groups (F= 12.776, P < 0.001). Moreover, at T4, the serum levels of TGF-β (F = 3286.548, P < 0.001) and IL-10 (F = 4628.983, P < 0.001) were significantly increased in the 38°C group, and the serum levels of TNF-α (F= 911.415, P < 0.001) and IL-1β (F = 322.191, P < 0.001) were significantly decreased in the 38°C group, compared with the levels in the 32 and 43°C groups.Conclusion:Force-air warming blankets set at 38°C maintained stable body temperature with less adverse outcome and effectively inhibited the inflammatory response in pediatric patients undergoing surgery for DDH.Clinical trial registration:ChiCTR1800014820; http://www.chictr.org.cn/showproj.aspx?proj=25240.