简介：Topretreatspermatvarioustemperaturesbeforeexposuretohumanpapillomavirus(HPV)16DNAfragmentsandtoassesstheefficiencyofHPVcarrierspermtotransfectcumuluscells.Methods:Cumuluscellsfromfollicularaspirateswereobtained,pooledanddividedintoculturedishescontainingSybyGold-stainedHPVDNAcarryingspernthatwereeitherpretreatedat4℃,37℃or40℃(n=5).Thecellswereincubatedin5%Co2inairmixtureat37℃for24hours.TheeffeiciencyofspermtotakeupfluorescentHPVDNAwasdeterminedathour0.Afterincubation.cumuluscellviabilitywasassessedusingtheeosinmethodandthepercentagesoffluorescentcumuluscellsdetermined.Results:Ouverhalfofallthecumuluscellsbecamefluorescentwiththehighestpercentageinthe37℃group.Spermpretreatedat4℃hadthegreatestamountofHPVDNAfragments.Totalspernmotilitywassimilarforthe3pretreatmentgroups.Therewerenodifferencesincumulusviabilityamongthegroups.Conclusion:Spermpretreatedat37℃transferredthegreatestamountoffluorescentHPVDNAfragmentstothecumuluscells.TheHPVDNAwasobservedinthenuclearandcytoplasmiccompartments.ThedatasuggestedthepossibilityofspermasavectorforthetransmissionofHPVDNAtothecumuluscellssurroundingovulatedoocytes,whichmightleadtoearlyimplantationfailures.