简介：BACKGROUND:Alongwithaging,antioxidaseactivitydecreasesandoxygen-derivedfreeradicalsgreatlyaccumulate,resultingincellularsenescence,orevencelldeath.Thisismanifestedbyhypomnesiaanddis-orderedmetabolismoffreeradicals.StudieshavereportedthatLongyanshenpolysaccharideshavethefunc-tionofantioxidationandimprovedbrainmemory.OBJECTIVE:ToobservetheeffectsofLongyanshenpolysaccharidesonfreeradicalmetabolisminbraintissuetoverifytheanti-agingmechanismsinsenescenceaccelerated-prone(SAMP8)mice.DESIGN,TIMEANDSETTING:Therandomized,controlled,biochemicalexperimentwasperformedintheDepartmentofPharmacologyandScientificExperimentalCenterofGuangxiMedicalUniversity(China)fromSeptember2005toJanuary2008.MATERIALS:FortySAMP8micewererandomizedintofourgroups:SAMP8controlgroup,aswellaslow-,mid-,andhigh-dosepolysaccharide,with10miceineachgroup.Tensenescenceaccelerated-resistant-prone(SAMR1)miceservedasthenormalcontrolgroup.Longyanshenpolysaccharides,extractedfromthemedicalplantLongyanshen,weresuppliedbytheDepartmentofPharmacology,GuangxiMedicalUniversity.Superoxidedismutase(SOD),glutathioneperoxidase(GSH-Px),malonaldehyde(MDA),nitricoxide(NO),andtotalproteintestkitwerepurchasedfromNanjingJianchengBioengineeringInstitute(China).METHODS:SAMP8micewereusedtoestablishadementiaanimalmodel.SAMP8andSAMR1controlmicewereadministered30mL/kgsaline.Thelow-,middle-,andhigh-dosepolysaccharidegroupswereadministered45,90,and180mg/kgLongyanshenpolysaccharides,respectively.Eachgroupwastreatedbyin-tragastricadministration,oncedaily,for50continuousdays.MAINOUTCOMEMEASURES:Onehourafterthelastadministration,mousebraintissueswerecollected,andretroorbitalbloodsamplingwasperformed.SpectrophotometrywasusedtomeasureSODandGSH-Pxactivity,aswellasMDAandNOconcentrationinseraandbrainsofSAMP8mice.RESULTS:

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简介：一个指责的地区的形态学和结构不仅表示现在的地位而且保存早seismo构造的出现的证据。现在的纸试图在地震估计早seismo构造的出现的词法以及表面下的证据巴罗穆拉·希尔，的容易的地区Tripura。构造地这在二度的地区V以内是Tripura-Mizoram褶层带和秋天的部分地震地图。在现在的研究，巴罗穆拉·希尔的构造的貌被使用卫星数据识别。为那个目的，LandsatTM短波红外线，红外线、近红外线的midwave为准备合适的乐队联合被选择。分类的A热红外线被用于最后的操作。从遥感操作，五清楚的构造的貌被发现。在领域工作岩石期间，样品为实验室测试被收集。岩石的薄节证明那个复杂压力模式在引起了强壮的seismo构造的出现的地质的过去期间被开发。从学习，这能被估计尽管paleo地震的出现的性质是几乎一样的，但是影响的水平通过空间、时间的规模变化了到期的不同物理性质和安排。当它似乎是，它从这个地方的地质的进化是更复杂的学习也是清楚的。构造、似地球形状的过程通过第三级、第四级的时期一起工作了并且为地形进化起了同等地重要的作用。

简介：Objective:TostudyexplorestheeffectofHLEConthesecretedproteinsofepithelialovariancancer(EOC)cells(SKOV3-PM4)withdirectionalhighlylymphaticmetastasis.Methods:Supernatantsoffourgroupsofculturedcells,namely,SKOV3(A),SKOV3+HLEC(B),SKOV3-PM4(C),SKOV3-PM4+HLEC(D),werecollected,andtheirproteinsweredetectedbyantibodyarraysandiTRAQ-2D-LC-MALDITOF/TOF/MS.SignificantlydifferentialproteinswerefurtheranalyzedviabioinformaticsandvalidatedinhumanserumsandcellmediaviaELISA.Results:ResultsofantibodyarraysandmassspectrometrydemonstratedthatGRNandVEGFAwereupregulatedingroupC(comparedwithgroupA),whereasIGFBP7andSPARCweredownregulatedingroupD(comparedwithgroupC).ComprehensivebioinformaticsanalysisresultsshowedthatIGFBP7andVEGFAwerecloselylinkedtoeachother.FurthervalidationwithserumsshowedstatisticalsignificanceinVEGFAandIGFBP7levelsamonggroupsofpatientswithovariancancers,benigntumors,andcontrolgroups.Twoproteinswereupegulatedinthefirstgroup.VEGFAinthecontrolgroupwasdownregulated.ForIGFBP,upregulationinthecontrolgroupanddown-regulationinthefirstgroupwerealsoobserved.Conclusion:TheHLECmicroenvironmentiscloselyassociatedwithdirectionalmetastasistolymphnodesandwithdifferentialproteinsincludingcellstromalproteinsandadhesionfactors.TheupregulationofVEGFAandGRNandthedownregulationofSPARCandIGFBP7arecloselyassociatedwithdirectionalmetastasistolymphnodesinEOCcells.

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简介：Toinvestigatethetreatmenteffectof2-seleniumbridgedβ-cyclodextrin(2-SeCD),aGPXmimic,onthestrokeofstroke-pronespontaneouslyhypertensiverats(SHRSP),fifty-twoSHRSPof8-weekoldwererandomlydividedintofourgroupsA,B,CandcontrolgroupD.TheratsofgroupsA,B,CandDweregiven1.0%-1.5%NaClmassfractionasdrinkingfluid.Afteronsetofstroke,groupsA,BandCweregivenorally16.05,160.5and1605mg*kg-1*day-1of2-SeCD,respectively,andgroupDwasgivenwaterfor2weeks.Theclinicalscoreofstroke,systolicbloodpressure(SBP),survivaltimeofratswererecordedandthehistopathologicexaminationsoftheirbrainandcarotidarteryweremadeafterdecapitation.Theclinicalscoresofstrokeaftertreatmentwith160.5mg*kg-1*day-1(GroupB)and1605mg*kg-1*day-1(GroupC)of2-SeCDare2.55±0.98and1.98±0.79,respectively,thoseareobviouslylowerthanthatofgroupD(3.41±0.83,p<0.01).ThesurvivaldaysingroupB(10.0±8.6)andgroupC(14.4±7.9)arelongerthanthatforgroupD(4.7±2.9,p<0.01).TheelectronmicroscopestudyshowedthattheendotheliumofcarotidarterywasneartonormalingroupBandgroupC,whileitwasseriouslyinjuredincontrolgroupDandmildlyinjuredingroupA.2-SeCDmayeffectivelybeusedtotreatthestrokeforSHRSP.

简介：Basementmembranedegradationandblood-brainbarrierdamageappearaftercerebralinfarction,severelyimpactingneuronalandbrainfunctioning;however,theunderlyingpathogeneticmechanismsremainpoorlyunderstood.Inthisstudy,weinducedcerebralinfarctioninstrokepronespontaneouslyhypertensiveratsbyintragastricadministrationofhigh-sodiumwater(1.3%NaCl)for7consecutiveweeks.Immunohistochemicalandimmunofluorescenceassaysdemonstratedthat,comparedwiththenon-infarctedcontralateralhemisphere,stroke-pronespontaneouslyhypertensiveratsonnormalsodiumintakeandWistar-Kyotorats,matrixmetalloproteinase-9expression,thenumberofbloodvesselswithdiscontinuouscollagenIVexpressionandmicrovesseldensityweresignificantlyhigher,andthenumberofcontinuouscollagenIV-positivebloodvesselswaslowerintheinfarctborderzonesofstroke-pronespontaneouslyhypertensiveratsgivenhigh-sodiumwater.Linearcorrelationanalysisshowedmatrixmetalloproteinase-9expressionwaspositivelycorrelatedwiththenumberofdiscontinuouslycollagenIV-labeledbloodvesselsandmicrovesseldensityincerebralinfarctsofstroke-pronespontaneouslyhypertensiverats.Theseresultssuggestthatmatrixmetalloproteinase-9upregulationisassociatedwithincreasedregionalangiogenesisanddegradationofcollagenIV,themajorcomponentofthebasallamina,instroke-pronespontaneouslyhypertensiveratswithhigh-sodiumwater-inducedfocalcerebralinfarction.

简介：Inthepresentstudy,theeffectofblockageofthecostimulatorysignalCD86attimeofimplantationontheexpressionsofTGF-β1,MMP-9,TIMP-3andPAI-1proteinsatthematernal-fetalinterfaceandtheoutcomeofpregnancyinmurineabortion-pronemodelwasinvestigated,inwhichtheCBA/JxDBA/2matingswereusedastheabortion-pronemodelandtheCBA/J×BALB/cmatingsusedasthenormalpregnantmodel.Thestudywasperformedinfollowingthreegroups:2groupsoftheabortion-pronemodel,whichwereexperimentalgroupandcontrolexperimentalgroup,and1groupofnormalpregnantmodel,andeachgrouphad10pregnantCBA/Jmiceexclusively.FemalepregnantCBA/Jmiceintheexperimentalgroupreceivedanintraperitoneal(i.p.)injectionof100μgofantimouseCD86mAbin200μlofPBSatday4.5ofgestation,andtheirrelevant-isotopematchedratIgG2bwasadministratedinthecontrolexperimentalgroupwiththesamedosageandatsametime.Forthenormalpregnantgroup,notreatmentwasgiven.ThepregnantCBA/Jmicewerekilledonday13.5ofgestation.Then,theembryoresorptionratewascalculatedandtheexpressionsofTGF-β1,MMP-9,TIMP3andPAI-1weredetectedbyusingimmunohistochemicalmethods.Itwasdemonstratedthattheembryoresorptionrateintheexperimentalgroupwassignificantlyreducedincomparisonwiththatinthecontrolexperimentalgroup(x2=7.441,P=0.006),buttherewasnosignificantdifferencewiththatinnormalpregnantgroup(x2=0.016,P=0.898).TheexpressionsofTGF-β1andPAI-1intheexperimentalgroupweresignificantlyincreasedincomparisonwiththatinthecontrolexperimentalgroup(P=0.010,P=0.003,respectively),withnosignificantdifferencefromthatinthenormalpregnantgroup(P=0.500).However,theexpressionofMMP-9intheexperimentalgroupwassignificantlyreducedincomparisonwiththatinthecontrolexperimentalgroup(P=0.012)withnosignificantdifferencefromthatinthenormalpregnantgroup(P=0.500).Theexpression