简介:Thenonchromatinproteinousresidueofthecellnucleuswasrevealedinourlaboratoryasearlyasin1948andthenidentifiedbylightandelectronmicroscopyasresidualnucleoli,intranuclearnetworkandnuclearenvelopebefore1960,Thisstructuretermedafterwardsas'nuclearresidue','nuclearskeleton','nuclearcage','nuclearcarcass'etc.,wasmuchlater(in1974)isolated,studiedandentitledas'nuclearmatrix'byBerezneyandCoffey,towhomthediscoveryofthisresidualstructureisoftenwronlyascribed.Therealhistoryofnuclearmatrixmanifestationisreportedinthispaper.
简介:China’snuclearindustryisgoingtomakegreateruseofitsREmetalstogivefullplaytoitsenergy-producingtechnology.TheChinaNationalNuclearCorporation(CNNC)willconcentrateonthedevelop-mentofREpermanentmagnets,REfluore-scence,andREceramics.Theseproducts,alsohighprioritiesinthedevelopmentplanfortheREindustryinthecomingsixyears,areallhi-techandenergy-savingproducts.
简介:SinceAugust2005,IranononesideandtheUnitedStatesandtheEuropeancountriesontheotherhavebeenlockedinarangleovertheIrannuclearissueandtensionhassteadilyescalatedtoacriticalpointasbothsidessticktoanuncompromisingposition.InearlierJune2006,inordertobreakthedeadlock,theEU,withU.S.blessing,onceagainofferedIrananewpackageofincentivesinan
简介:Weisolatedandpurifiedmitochondriafrommouseliversandspinachleaves.WhenaddedintoeggextractsofXenopuslaevis,theycausednucleiofmouselivertoundergoapoptoticchanges.Chromatincondensation,marginationandDNAladderwereobserved.Afterincubatingisolatedmitochondriainsomehypotonicsolutions,andcentrifugingthesemixturesatmghspeed,wegotmitochondrialsupernatants.Itwasfoundthatintheabsenceofcytosolicfactor,thesupernatantalonewasabletoinduceapoptoticchangesinnuclei.Theeffectivecomponentswerepartlyofprotein.DNAfragmentationwaspartlyinhibitedbycaspaseinhibitorsAC-DEVD-CHOandAC-YVAD-CHO.Meanwhile,caspaseinhibitorsfullyblockedchromatincondensation.Primarycharacterizationofthenuclearendonuclease(s)inducedbymitochondrialsupernatantswasalsoconducted.ItwasfoundthatthisendonucleaseisdifferentfromendonucleaseG,cytochromec-inducednuclease,orCa^2+-activatedendonuclease.
简介:Thenuclearmatrixattachmentregions(MARs)andthebindingnuclearmatrixproteinsinthe5'-flankingcisactingelementsofthehumanε-globingenehavebeenexamined.UsinginvitroDNA-matrixbindingassay,ithasbeenshownthatthepositivestage-specificregulatoryelement(ε-PREII,-446bp--419bp)upstreamofthisgenecouldspecificallyassociatewiththenuclearmatrixfromK562cells,indicatingthatε-PREIImaybeanerythroidspecificfacultativeMAR.IngelmobilityshiftassayandSouthwesternblottingassay,anerythroid-specificnuclearmatrixprotein(ε-NMPk)inK562cellshasbeenrevealedtobindtothispositiveregulatoryelement(ε-PREII).Furthermore,wedemonstratedthatthesilencer(-392bp--177bp)upstreamofthehumanε-globingenecouldassociatewiththenuclearmatricesfromK562,HELandRajicells.Inaddition,thenuclearmatrixproteinspreparedfromthesethreecelllinescouldalsobindtothissilencer,suggestingthatthissilencerelementmightbeaconstitutivenuclearmatrixattachmentregion(constitutiveMAR).Ourresultsdemonstratedthatthenuclearmatrixandnuclearmatrixproteinsmightplayanimportantroleintheregulationofthehumanε-globingeneexpression.
简介:Coactivatorsandcorepressorsregulatetranscriptionbycontrollinginteractionsbetweensequence-specifictranscriptionfactors,thebasaltranscriptionalmachineryandthechromatinenvironment,Thisreviewconsidertheaccessofnuclearandsteroidreceptorstochromatin,theiruseofcorepressorsandcoactivatorstomodifychromatinstructureandtheimplicationsfortranscriptionalcontrol.Theassemblyofspecificnucleoproteinarchitecturesandtargetedhistonemodificationemergeascentralcontrollingelementsforgeneexpression.
简介:<正>TheceremonyforissuingtheinstrumentofratificationonfirstfuelloadingtoUnit.1ofTianwanNuclearPowerPlantwasholdonOctober23thisyear.TheStateEnvironmentalProtectionAdministrationissuedthisinstrumentattheceremony.Onthesameday,ninety-fivegroupsofPWRfuel
简介:ConstructionofLing’aoNuclearPowerPlantinGuangdongProvince,thecountry’ssecond-largestnuclearpowerprojectbetween1996-2000,chalkedupnotableprogressthisyear.OnJuly,15,allforeignsuppliersintheprojectweregivenofficialnoticethatbothsidesshouldstarttoimplementthecontracts."Themovemarkedasignificantstepforwardintheproject’sconstruction,"saidZengWenxing,GeneralManagerofLing’aoNuclearPowerCoLtd,setupinSeptember
简介:客观:在在人的创伤的奔流和正常透镜的上皮的房间之间的原子factor-KB(NF-κB)的表示学习差别。方法:全部的RNAof前面的囊标本从受不了创伤的奔流做半量的RT-PCR并且在在他们之间的NF-κB的表示进行差别的分析的正常cadaveric眼睛施主和那些在显微镜下面被拿。结果:作为与在正常控制组的0.8337的平均数相比,NF-κB的表示等价物为在创伤的奔流患者的透镜的上皮的房间是0.9074,并且差别具有显著意义(t=2.447,P<0.05)因此。结论:NF-κB是可能的对必要的一种抄写因素维持正常透镜的上皮的房间的新陈代谢。在创伤的奔流患者可得到的更高的NF-κB“透镜的上皮的房间工具NF-κB具有到创伤的奔流的出现和开发的可能的关联的s。
简介:Since2003,theIrannuclearissuehasattractedworldwideattention.Thisistheworld’smostprotractedandmostinfluentialconfrontationbetweenasuperpowerandabigIslamiccountrysincetheendoftheColdWar.AstheIrannuclearissuehasabearingonthemaintenanceoftheinternationalnuclearnon-proliferationsystem,theUnitedNations,theInternational
简介:IntheGCMwestudysomepropertiesofπmesonastheGoldstonebosonsinanuclearmatterwithfinitedensity.Usingtheeffectiveactioninanuclearmatter,wecalculatethedecayconstantandπmassasfunctionsofthechemicalpotential.Therelationbetweenthechemicalpotentialandthedensityofanuclearmatterisfirstlygivenhere.Wefindthatfπandmπmonotonouslydecreaseasnuclearmatterdensityincreases.Theresultisconsistentwiththeusualassumptionthatthechiralsymmetryisgraduallyrestoredasthedensityofanuclearmatterincreases.