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简介：调查的目的在里面vitro抗氧化剂活动和methanolic叶的全部的酉分的内容Nyctanthesarbor-tristisL提取。(NA)。样品在vitro抗氧化剂方法用五被测试的方法(1，1-diphenyl-2-picryl肼基清除活动(DPPH)，氢氧根激进清除的活动(-OH),氮的氧化物清除活动(没有)，superoxide激进清除的活动，并且总计抗氧化剂活动)评估在里面NA和全部的酉分的内容(Folin-Ciocalteu方法)的vitro抗氧化剂潜力。摘录看好免费激进的清除性质哪个作为IC50价值计算。methanolic摘录的结果IC50(半最大的禁止的集中)被发现是57.93?g牴慥整?慲獴愠?潢桴搠獯獥

简介：Objective:TheaimofthepresentstudywastoinvestigateantioxidantandtheanticancerigenactivityofamethanolextractfromArtemisiaprincepsvar.orientalis(APME),awell-knowntraditionalherbalmedicineinAsia,inhepatocellularcancercells.Methods:ToevaluatetheantioxidantactivityofAPME,reactiveoxygenspecies(ROS)andtheantioxidantenzymes,superoxidedismutase(SOD)andcatalasewereinvestigatedinHepG2cellsexposedtoAPME(5,100,and200μg/mL)for72h.Then,toevaluatetheanticanceractivityofAPME,weinvestigatedtheproliferationandapoptosisinductionofHepG2andHep3BcellsexposedtoAPME(1-200μg/mL)for24,48,and72h.Results:APMEdose-dependentlyreducedthegenerationofROSinthepresenceofH2O2comparedwithcontrolcells.Furthermore,itincreasedcatalaseandSODactivity.Moreover,APMEinhibitedcellproliferationinadose-andtime-dependentmanner,butatconcentrationslowerthan100μg/mL,theinhibitionwaslessdose-dependentthantime-dependent.HepG2andHep3Bcellsexposedto5,100,and200μg/mLAPMEfor72hunderwentcellcyclearrestandapoptosis.ExposuretoAPMEresultedinasignificantincreaseinthenumberofcellsinG1phaseandadecreaseintheG2/Mphasecellpopulation.Inaddition,APMEinducedP53expressionofHepG2cellsinadose-dependentmanner,andplayedaroleinthedownregulationofBcl-2andupregulationofBaxinbothHepG2andHep3Bcells.Conclusions:TheseresultsindicatethepotentialroleofAPMEasanantioxidantandanticancerigenagentinhepatocarcinomacelllines.