简介：根据Ensembl、Genbank登录的鱼类cat、gapdh和gst基因的CDS序列设计普通PCR扩增引物,寻找食蚊鱼的cat、gapdh和gst基因的cDNA片段,并根据定量引物设计要求设计出相应的SYBRGreenI荧光定量RT-qPCR引物,建立了食蚊鱼cat、gapdh和gst基因的SYBRGreenI荧光定量RT-qPCR方法。该方法在104~108数量级范围内有良好线性关系（R=0.999~1.000）;熔解曲线显示扩增产物特异性良好,均为单一峰值;质粒标准品最高浓度与最低浓度的批内试验变异系数与批间试验变异系数均低于2%。利用该方法监测和评价环境污染物对水生生物的影响,选择了水体中常见典型药物污染物——双氯芬酸,研究其对食蚊鱼抗氧化基因表达的影响。结果表明,雌性食蚊鱼暴露在不同浓度双氯芬酸钠（0.005、0.05、0.5和5mg·L-1）24h后,其肝脏cat、gapdh和gst的mRNA呈现显著变化,相对于对照组,在低浓度0.005mg·L-1时,cat与gstmRNA的表达量均有极显著上升（p〈0.01）,而其它浓度均极显著下降（p〈0.01）。试验表明该方法具有快速、精确、灵敏度高的优点,可为利用该类小型鱼类的原位污染物的生物监测和生态毒理评价提供有力的技术支持。

简介：基于谷胱甘肽（GSH）解毒作用探讨了微囊藻毒素-RR（MCRR）在不同动物肝脏和肾脏合作下的代谢机制。通过人工合成MCRR的谷胱甘肽代谢物（MCRR-GSH）,腹腔注射至鲫鱼和大鼠体内,利用液相色谱串联质谱技术（LC-MS/MS）定量检测MCRR-GSH及其下游半胱氨酸代谢物（MCRR-Cys）在组织内的代谢动力学变化。在72h的暴露实验中,实验组鲫鱼和大鼠体内均定量检测到MCRR-GSH和MCRR-Cys。MCRR-GSH在肾脏中的浓度显著高于其他组织（P〈0.05）,鲫鱼和大鼠体内累积浓度分别是（0.161±0.001）和（0.116±0.005）μg·g^-1DW。同样的,MCRR-Cys主要分布于鲫鱼和大鼠的肾脏组织。鲫鱼肾脏中MCRR-Cys的浓度出现明显的波动,而肝脏和胆汁内的MCRR-Cys浓度却呈现出上升的趋势;大鼠肾脏内MCRR-Cys的浓度呈缓慢下降的趋势,浓度范围为（8.899±0.817）μg·g^-1DW至（3.336±0.263）μg·g^-1DW。基于以上结果推测,微囊藻毒素在肝脏和肾脏合作下的解毒过程为：MC在肝脏内经GSH结合作用生成的代谢物MC-GSH随血液循环转运至肾脏,在肾脏内MCGSH快速地转化为下游代谢物MC-Cys以促进排泄。

简介：Afterthe1992UNConferenceonEnvironmentandDevelopmentinRiodeJaneiro,theconceptofsustainabledevelopmenthasbeenwidelyrecognizedallovertheworld.Morethan100countries,includingChina,haveadoptedsustainabledevelopmentstrategiesaccordingwiththeirownsituations.For20years,

简介：Theproblemofclimatechangeisaglobalchallenge.Itiscloselyassociatedwithsocialdevelopmentandhumansurvival,andithasasignificantimpacttoallcountriesonenergydevelopment,economiccompetitiveness,technologicalinnovation,andwayoflife.Inrecentyears,withtherapideconomicdevelopmentinChina,thereisarumorthattherapidgrowthofChina'scarbondioxideemissionoffsettheeffortsoftheinternationalcommunityinreducingemissions,andChinashouldbeartheinternationalresponsibilitycorrespondingtoitssignificantroleingreenhousegasemission,whichobviouslyareunfairandnotobjective.Asthispaperreveals,'Chinaenvironmentresponsibility'thatisthesocalled'Chinaenvironmentthreat'ortheories,ChinahasmadeapositivecontributiontoaddressingtheclimatechangeinthepastandChinawillstillbethebackboneontheprotectionofglobalclimateinthefuture.

简介：7S管理方法起源于日本．在日本叫5S．内容包括整理（SEIRI），整顿（SEITON）、清扫（SEISO）清洁（SEIKETSU）素养（SOYOU）。此5项内容在日文的罗马发音中均以“S”为开头．故简称为5S。5S活动是具体而实在的．不仅让员工一听就懂而且很容易实行．推行5S的目的就是要为员工创造一个干净，整洁，舒适．合理，安全的工作场所和空间环境。

简介：Inrecentyears,carbonemissionshavegraduallyevolvedfromanenvironmentissueintoapoliticalandeconomicone.Carbontariffhasbroughtaboutnewtradebarriersofdevelopedcountries,andinordertoenhancetheindustrialcompetitivenessofdevelopedcountries,itwillproduceunfavorableimpactondevelopingcountries.Concentratedonthemanufacturingindustry,whichisthemostintensivehigh-carbonindustryinChina’sexportstructure,thisarticlestudiestherelationshipbetweencarbontariffpolicyandindustrystructureofexporttradeandbuildsuparelationbetweenclimatechangeandinternationaltrade.First,bymeansofestablishingapartialequilibriummodel,itappliesgeometricanalysisandmathematicalanalysistocomputetheimpactonChina’smanufacturingexporttradeandtheconsequencesoftheintroductionoftheUScarbontarifftoChina’smanufacturingindustrythathasalreadyimposedadomesticshippingcarbontax.Furthermore,withtheapplicationoftheGTAPmodel,itestimatestheoveralleconomicandwelfareeffectsonChina’smanufacturingindustryiftheUSandEuropeintroducecarbontariffbymeansoffourways,andthenanalyzestheinfluenceonChina’smanufacturingindustryexportstructureandsocialwelfareaswell.TheresultshowsthattheintroductionoftheUScarbonimporttarifflowersChina’sexportpriceandexportvolume,andtheimplementationofadomesticcarbontaxjustifiesahigherexportpriceandalowerexportvolumeforChina.However,thedegreeofexportreductionissmallerthanthatundertheeffectoftheUScarbontariff.InthecaseofdevelopedcountriesimposingcarbontariffonChina’senergy-intensiveindustries,suchaschemicalrubberproducts,oilandcoal-processingindustryandpaperindustry,whoseexportwouldbereduced,thenegativeimpactonthepaperindustryistheseverest,whichwilldecreasethepaperindustry’sexportrangingfrom1.79%to6.05%,whereastheotherindustries’exportwillincrease.Anyhow,itwillpromoteChina�

简介：AllsevenemissionstradingpilotsinChinaoperateindependently.Onechallengefacingmostofthemisthelowinclusionthresholdsforenterprisesandthefewtotalcoveredemissions,whichnegativelyinfluencestheeffectsoftheemissionstradingsystems(ETSs).Somepilotsites,suchasGuangdong,Hubei,TianjinandBeijing,haveindicatedtheirwillingnesstolinktheirschemeswithothers.ETSlinkingcouldexpandschemecoveragesandthereforehelptoreducetheoverallcostsofachievingthelinkedschemes’emissionscontroltargets.Linkingcouldalsohelptoaddresstheissuesofcarbonleakageandreducepricefluctuations.Thepotentialbenefitsandfeasibilityoflinkingdifferentpilotsystemsareanalyzedinthisarticle.Thesevenpilotregionsareatdifferentstagesofsocialandeconomicdevelopment,withsignificantdifferencesintotalemissionsandemissionsstructuresaswellascarbonabatementpotentialsandcosts.Throughlinking,more-developedregionssuchasBeijing,ShanghaiandShenzhen,whicharetypicallyconsideredtofacehighermitigationcosts,willhavetheopportunitytoachievetheiremissionscontroltargetsbypurchasingcarbonunitsfromless-developedregions,whichwillearnfinancialrevenuesfromsellingtheunits.Torealizethiswin-winresult,aseriesofpolicyandtechnicalbarriersatboththecentralgovernmentandpilotgovernmentlevelsneedstobeovercome.Establishingaunifiednationalemissionstradingmarketwouldappeartobetheidealsolutiontothesechallenges,butitwilltakeconsiderabletimeandwillnotbetheshort-termsolution.Intheabsenceofaunifiednationalscheme,itisrecommendedthatthecentralgovernmentencouragepilotschemestolink,thatitdevelopscorrespondingnationalpoliciestosupportthelinkingeffortsandthatthepilotschemesthatareintendedtobelinkedcoordinateoncertaindesignelements.Basedonthecoordinatingneed,themajorelementsofanETScanbedividedintofourcategories:elementsthatneedmutualrecognition(capset

简介：I'mverypleasedtobehereatthissidemeeting,China'sGreenTransformation:Efforts,PracticesandFuture,co-sponsoredbyACCA21andUNDP.SincetheUNConferenceonEnvironmentandDevelopmentin1992,peoplearoundtheworldhavemadenumerous

简介：ForChina,greenindustrialrevolutioninducedbyglobalclimatechangeposesnotonlythegreatestchallenge,butalsothegreatestopportunity.IntheperspectiveofChina'sbasicnationalconditions,andespeciallyitsnaturalconditions,China'sgreendevelopmentistheinevitablepathofchoicefortherealizationofsustainabledevelopmentandscientificdevelopment.TheessenceofChina'smodernization2050isgreenmodernization,takingthethree-stepstrategytowardsChina'sowngreendevelopmentandenergyconservationandemissionreduction.Incombinationwiththe12thFiveYearPlan,itsinnovativepositioningis'greendevelopmentplan'.

简介：Producinggoodsandservicesallneedswaterconsumption.Thewaterusedintheprocessofanagriculturalorindustrialproductiscalledthe"VirtualWater"containedinthisproduct.Throughinternationaltrade,water-scarcecountriesandregionscouldpurchasewater-intensiveproducts--especiallyfoods,fromwater-richcountriestobalancetheirwaterdeficitsandachievewatersafety.Chinaisoneofthe13mostwater-deficitcountrieswhosewatersafetyhavebeenseverelychallenged.Thispapergeneralizedtherecentglobalresearchdevelopmentandmadeabriefintroductionaboutthemethodscalculatingvirtualwatercontentinspecificproducts.Asacasestudy,wequalifiedChina'sannualvirtualwaterflowsfromyear2000to2002withtradeincrops,andendedwithsomepolicyadviceforapplicationandpracticeofvirtualwaterstrategy.

简介：TheEU,theUnitedStatesandothereconomies,withtheintentiontoimplementunilateraltrademeasuresBorderCarbonAdjustments,imposeemissionreductionpressureondevelopingcountries.Onceimplemented,themeasureswillhavegreatimpactonChina'sforeigntrade.Usingtheinput-outputtablein2007,thispaperhadanalyzedtheinfluencesonChina'sforeigntradeasawholeandsub-sectorsinthreetaxratesscenarios.TheresultsshowedthatthetarifflevelofChina'sexportswillincreaseby3.6%-6.3%ifthetaxwasleviedonexportsembodiedemissions,andby1.0%-1.7%ifleviedonexportdirectemissions.In2007,theformertotalamountofcarbontaxwasaboutUS$42.6-73.0billion,4timesthatofthelatter.Basedonexportembodiedemissions,sectorslargelyinfluencedwerenon-traditionalenergyintensiveones,suchastextile,etal.Thesesectorsshouldbeencour-agedtocarryoutindustrialupgrading,raisingthevalue-addedofexportgoods,andreducingtheirembodiedemissionsbyreductionofenergyintensity.Takingintoaccountofthecomplexityofdatacollection,thetaxleviedonproductsdirectemissionismoreoperational.Theresultsshowedthatthefivetopsectorsmostaffectedwereotherchemicalmaterials,processingofpetroleumandnuclearfuel,coking,smeltingandrollingofferrousmetalandtextile.Mostofthemwereenergyintensivesectors.Therefore,adjustingexportproductsstructure,andcontrollingtoofastdevelopmentofenergyintensiveindustriesarealsoimportantstrategiesinChina.

简介：Theindustrialsectorisusuallythelargesteconomysectorforcarbonemissionsinmanycountries,whichmadeitthesectorwithgreatestpotentialforcarbonreductionalthoughtheprocessdurationmightbeverylong.StudyingthepotentialofindustrialemissionreductionhasgreatsignificanceinestimatingthecarbonemissionpeakofChinaontheonehand,andadjustingitsstrategyininternationalclimatechangenegotiations.Byemployingtheeconomicaccountingmethod,thisarticleestimatestheemissionreductionpotentialofChina’sIndustrialsectorfortheperiodof2010-2050.Itrevealsthat,taking2030astheyearwhentheemissionreachesthepeak,thetotalreductioncanbe8.38billiontons(bts)fortheperiodof2010-2030,with3.12btsfromstructuralreductionwhile5.26btsfromintensityreduction.Afterwards,reductionwillcontinuewithatotalamountof6.59btsfortheperiodof2030-2050,wherethestructuralreductionaccountsfor2.47bts,andintensityreduction4.115bts.Ifbothindustrialandenergyconsumptionstructuresareimprovedduringtheaboveperiod,thereductionpotentialcanbeevengreater,e.g.theemissionpeakcanarrivefiveyearsearlier(intheyearof2025)andthepeakvaluecandeclinebyabout8%ascomparedtotheoriginalestimation.Reviewingthetrajectoryofemissionchangesindevelopedcountriesindicatesthattheindustrysectorcancontributetotheoverallreductiontargetsthroughthedualwheelsofstructuralreductionandintensityreduction,evenbeyondtheemissionpeak.Thisarticleconcludeswiththefollowingpolicysuggestions.(1)OurestimationontheemissionpeakoftheindustrialsectorsuggeststhatChinashouldavoidanycommitmentearlierthan2030onthetimelineoftheoverallemissionpeak;(2)thegreatpotentialofindustrialemissionreductioncanimprovethesituationofChinainclimatechangenegotiation,wheretheintensityreductioncanserveasanimportantpolicyoption.(3)Reductionpotentialcanbefurtherenhancedthroughtechnologyadv

简介：Aspartofacomprehensiveenvironmentalmanagementsystem,manycountriesestablishemissioncontroltargetsformassemissionsofapollutant.Suchtargetsareoftenthekeyobjectiveofanenvironmentalpolicy,suchasanemissiontradingprogram.InChina,however,itismorethanjustanobjectiveofoneparticularpolicy;ithasbecomeaconceptthathasinfluencedmanynationalenvironmentalpoliciesandactivities.Theobjectiveofthisarticleistoreviewtheimplementationofthetotalemissioncontrolpolicyinthepast10yearsandexploreemergingissuesinitsimplementation.Thearticlehasthreesections:asummaryoftheimplementationexperience,issueswiththedesignandimplementationofthepolicy,andpolicyrecommendations.

简介：

简介：Chinahaswitnessedrapideconomicdevelopmentsince1978,andduringthetime,energyproductionandconsumptiondevelopedatatremendousspeedaswell.EnergyefficiencywhichcanbemeasuredbyenergyconsumptionperunitofGDP,however,experiencedcontinuousdecrease.Theoretically,thechangeofenergyefficiencycanbeattributedtoindustrystructuralchangeandtechnologicalchange.InordertoexplainthetransformationofChineseenergyefficiency,weadoptlogarithmicmeanDivisiaindextechniquestodecomposechangesinenergyintensityintheperiodof1994-2005.Wefindthattechnologicalchangeisthedominantcontributorinthedeclineofenergyintensity,butthecontributionhasdeclinedsince2001.Thechangeinindustrystructurehasdecreasedtheenergyintensitybefore1998,butraisedtheintensityafter1998.Decomposedtechnologicaleffectsforallsectorsindicatethattechnologicalprogressesinhighenergyconsumingindustriessuchasrawchemicalmaterialsandchemicalproducts,smeltingandpressingofferrousmetals,manufactureofnon-metallicmineralproductsandhouseholdcontributearetheprincipaldriversofChina'sdecliningenergyintensity.

简介：Circularagricultureisanessentialwaytorealizethepositiverecyclingofecologyandharmoniousdevelopmentofthecountryconstruction.ThesustainabledevelopmentofBeijing'smountainareasdependsonthebackgroundandorientationofecologicaleconomy.Beijing'smountainareasbeartheresponsibilityofprotectingtheecologicalsafetyofthewholeBeijingdistrictandalsotheexplorationofcircularagricultureinmountainareasisveryimportanttopromotethesustainableuseofagricultureresources.Beijing'smountainareashavepossessedsomepracticalexperienceinrecyclingagriculturedevelopment.Throughsummarizingthedevelopmentmodeandanalyzingthedevelopmentpractice,thearticleputsforwardthreekeysegmentsofpromotingBeijingcircularagriculturedevelopment:strengthenpropagate,developsuperiorityandbuiltmanagementmechanism.

简介：研究了华北平原某铅冶炼厂附近农田33个土壤样品中重金属积累对土壤酶活性的影响。结果表明,样品中Pb和Cd全量的平均值分别为144和5.59mg·kg-1,DTPA态Pb和Cd含量平均值分别为54.1和0.964mg·kg-1,均超过了未污染农田潮土的正常范围,而Cu、Ni和Zn的有效性和全量与未污染土壤接近;土壤过氧化氢酶活性与DTPA态Pb和Cd含量、全Pb含量均呈显著的负线性关系（P〈0.01）;与磷酸酶和脲酶相比,土壤脱氢酶活性更易受到土壤中Pb和Cd积累的影响;随DTPA-Ni含量增加,土壤蛋白酶和碱性磷酸酶活性增加（P〈0.1）;土壤脲酶活性与重金属全量或有效态重金属含量无显著相关性（P〉0.1）。以上结果说明,利用土壤过氧化氢酶和脱氢酶活性能够表征基本性质较为一致的土壤中重金属污染程度;与重金属全量相比,有效态重金属对土壤酶活性影响更大。

简介：Basedonthetotal-factorenergyefficiencyframework,thispapercalculatesChina'sindustrialenergyefficiencyandCO2emissionsreductionpotentialfrom2000to2009byutilizingthedirectionaldistancefunctionanddataenvelopmentanalysis.Theempiricalresultsshowthat:China'sindustrialoverallenergyefficiencyisrelativelylowerwhiletheemis-sionsreductionpotentialisrelativelygreater,giventheoptimumproductionfrontier.Significantindus-trialdisparitiesofenergyefficiencyandemissionsreductionpotentialexist.Energyefficiencyandemis-sionsreductionpotentialsignificantlyshowdifferenttendenciesofindustrialdynamicvariation.ThispapersuggeststheChinesegovernmentimposedifferentialcarbontaxes,flexiblyutilizecarbonmarketmecha-nism,strengthenenergy-savingtechnologicalR&D,promotetheutilizationofrenewableenergy,andstrengthenenvironmentalsupervisionandregulation,soastoimproveChina'sindustrialenergyefficiencyandreduceCO2emissions.

简介：Chinaispreparingtoestablishanationwidecarbonmarketin2017,andinordertofacilitatethisgoal,sevenpilotcarbonmarketshavebeenunderstudyforthepastfewyears.ThispapersummarizestheoperationexperienceandchallengesofthesevenpilotcarbonmarketsinChina.Ithasbeenwidelyacceptedthattheessenceofacarbonmarketistosolveenvironmentalproblemsthroughmarketmechanisms,withenvironmentalbenefitbeingthefundamentalpurpose,marketmechanismbeingthekeymeasure,andpoliciesandregulationsbeinganimportantguaranteeforanorderlycarbonmarket.Therefore,thispaperconstructsanevaluationindexsystemcomposedof34detailedsub-indexesinthreedimensions,suchasenvironmentalconstraintforce,marketresourceallocationability,andsupportingpoliciesandfacilitycompleteness.Throughanalyzingtheoperationdatafrom2013to2016,theweightsofthesub-indexesareobtained.Inaddition,thestudyobtainsexperts’opinionsfromover10carbonpermitsexchanges,consultancyfirmsandresearchinstitutionsinChina,andconductsacomprehensiveevaluationonthedevelopmentdegreeofthesevenpilotcarbonmarkets.ResultsshowthatthepilotcarbonmarketsthatincludeprivateSMEsasthecoveredentitiesforemissionscontrolpresentrelativelyhigherenvironmentalconstraintforce.Buttoomanycoveredentitiescouldincreasethedifficultyofmarketperformancemanagement,whilethepilotsthatincludehighenergy-consumingstate-ownedenterprisesastheentitiesforemissionscontroldemonstrateaphenomenonof'highmarketcomplianceratewithlowtradingvolume'.TheresourceallocationcapabilityofChina’scarbonmarkethasnotbeeneffectivelybroughtintoplay,andlowdegreeofmarketparticipationhasbecomeanimportantconstraintfactorformarketdevelopment.Duetothelackoflawsandregulationsatthenationalmacro-level,thelegallybindingforceofthepilotmarketsconstructionisobviouslyinsufficient,andthesupportingpoliciesare

简介：Climatechangeandurbanizationissuesarethetwokeyfactorsthatmakehumansliabletobeaffectedbydisasters,whichareoverlappedinurbanagglomeration.ThefivebigurbanagglomerationsofChinawithstrongeconomicpoweraretheimportantenginesfornationaleconomicandsocialdevelopment.However,beinginthesea-landmutualinteractionbeltswithavasthazard-bearingbody,theyareaffectedbysea-landcompounddisasters,andareliabletosufferheavydisasterlosseswithclimatechange.Itissuggestedthatgovernmentdepartmentsconcernedshouldfullyrecognizetheimpactofclimatechangeoncoastalurbanagglomerations,proposestrategiesassoonaspossible,andintegratetheimpactofclimatechangeandadaptationcountermeasuresintothevariouskindsofsocial-economicdevelopmentplansforcoastalurbanregions.