简介:利用江苏南京、安徽宣城两地的水稻田间试验数据和气象资料,对ORYZA2000模型基本作物参数进行调整,包括不同发育阶段的发育速率、干物质分配系数、比叶面积等。两试验点的作物营养生长参数(DVRJ)和生殖生长参数(DVRR)差异很大,反应了模型的区域差异性。模拟效果均能准确反应叶面积指数、生物量的动态变化过程,在地上部生物量的模拟准确度最高。两试验点的叶面积指数、地上部生物量、绿叶生物量、茎生物量和穗生物量的归一化均方根NSMSE值分别为9%、19%、18%、13%、25%和16%、25%、17%、19%、24%,因而南京试验点的模拟效果比宣城好,参数更具区域适应性。
简介:Genomicinsituhybridization(GISH)isapowerfultooltocharacterizeparentalchromosomesininterspecifichybrids,includingthebehaviourofautosynapsisandchromosomepairing.ItwasusedtodistinguishthechromosomesofOryzasativafromwildspeciesinaspontaneousinterspecifichybridandtoinvestigatethechromosomepairingatmetaphaseIinmeiosisofthehybridinthisstudy.Thehybridwasatriploidwith36chromosomesaccordingtothechromosomenumberinvestigatedinmitosisofroottips.DuringmetaphaseIofmeiosisinthehybrid,lesschromosomepairingwasobservedandmostofthechromosomesexistedasunivalent.BasedonGISHandFISH(Fluorescentinsituhybridization)analyses,thechromosomesofthehybridwerecomposedofgenomesA,BandC.Thus,itwasbelievedthatthehybridwastheresultofnaturalhybridizationbetweencultivatedriceandwildspeciesO.minutawhichwasplantedinexperimentalfields.
简介:以便揭示在二CCDD染色体种,Oryzaalta和Oryzalatifolia之间的起源和进化关系,在situ杂交(鱼)的荧光被采用从O与C0t-1DNA分析二种的染色体。alta作为一根探针。Karyotype比较地也在O之间被分析。alta和O。latifolia基于他们杂交的类似的乐队模式发信号。在O之间有高相同和靠近的关系。alta和O。然而,在杂交之间的区别表明的latifolia也是清楚的。C0t-1DNA被证明是种类--并且染色体类型特定。C0t-1DNA鱼能是更有效的分析在不同种类之间的genomic关系,这被建议。根据在二allotetraploidy种之间的高度并且中等重复的DNA序列的比较分析,O。alta和O。latifolia,可能的起源和Oryza的allotetraploidy的进化机制被讨论。
简介:WedescribedtheconstructionofBACcontigsofthegenomeofaindicavarietyofOryzasativa.GuangLuAi4.Anentirerepresentative(Sixfoldcoverageofricechromosomes)andgeneticallystableBAClibraryofricegenomeconstructedinthislabhasbeensystematicallyanalysedbyrestrictionenzymefragmentationandpolyacrylamidegelelectrophoresis.Andalltheimagesthusobtainedweresubjecttoimage-processing,whichconsistedofpreliminarylocationofbands,cooperativetrackingoflanesbycorrelationofadjacentbads.aprecisedensitometricpass,alignmentatthemarkerbandswiththestandard,optionalinteractiveediting,andnormalizationoftheacceptedbands.ThecontigsweregeneratedbasedontheComputerSoftwarespeciallydesignedforgenomemapping.Thenumberofcontigswith600kbinlengthonaveragewas464.ofcontigswith1000kbinlengthonaveragewas107;ofcontigswith1500kbinlengthonaveragewasConstructionofOryzaSativagenomecontigs.23.Therefor,allthecontigswehaveobtainedampuntedupto420megabasesinlength.Consideringthesizeofricegenome(430megabased),thecontigsgeneratedinthislabhavecoverednearly98%ofthericegenome.Wearenowintheprocessofmappingthecontigstochromosomes.
简介:在大多数谷物庄稼,phytic酸是磷的主要存储形式,它能减少磷酸盐的简历可获得性。phytase的转基因的表示被认为是在转基因的植物免除磷酸盐phytate的一个有效方法。在这研究,工厂表示向量,包含重组体phytase基因由玉米ubiquitin(Ubi)开车倡导者经由Agrobacterium-mediatedtransformation被构造并且介绍进一个精英米饭变化。在实验期间,15根独立转基因的米饭线的一个总数被改革。PCR和南部的污点的结果显示目标基因集成于转基因的米饭工厂的染色体。而且,提取fromtheimmature几根转基因的线播种的全部的RNA的RT-PCR分析证明重组体phytase基因能通常被表示。无机的磷内容,两个都比在untransformed野生型在转基因的工厂在成熟种子和叶是显著地更高的。
简介:生物信息的途径补充了试验性的努力到inventorize植物miRNA目标。我们执行了产生通常认为的miRNA的一张全面的表的transcriptome指向的米饭(Oryzasativa)的全球计算分析。我们的预言(684个唯一的抄本)证明米饭miRNAs调停包括抄写(41%)的多样的功能的规定,催化作用(28%),绑定(18%),和transporter活动(11%)。在预言的目标之中,61.7%点击在编码区域,将近72%目标让独居的miRNA撞击。学习预言了通常认为地调整象压力反应,催化作用,和绑定一样的函数的34miRNAs的超过70个新奇目标。非常,目标的一半(55%)在O之间被保存,这被观察。sativaindica和O。sativa装饰用的梨树。31个miRNA家庭的成员被发现拥有在米饭之间并且至少的保存目标另外的草家庭成员之一。大约44%唯一的目标在二个不一样的miRNA预言算法之间是普通的。跨种类的保存和算法的一致的如此的程度在在这研究预言的米饭miRNA目标的表授与信心。
简介:Toobtainasuitableconditionforelectroporationtransformationinindicarice,the10-day-oldimmatureembryoswereselectedforoptimizationexperiments.Theresultsshowedthatonepulseat850V/cm,950μFcapacitance,200μLelectroporationbufferwith70mmol/Lsodiumglutamate,100μg/mLplasmid,50μg/mLcarrierDNA,20embryospercuvette,0°CtreatmentandCCmediumwerethebestparameters,whichnotonlyimprovedthetransformationefficiencyto30.89%,butalsoamelioratedtheembryosurvivalratioto95.92%.Tofurtherverifythepracticabilityofthiscondition,theembryosfromanotherindicaricevarietyandaricetypeIImetallothionein-likegene(OsMT2bL)promoter::mgfp5::gusAconstructweretested,andspecificGUSexpressionontheembryoswasvisualizedbyhistochemicalstaining.TheresultsshowedthattheGUSexpressionontheembryosactivatedbytheOsMT2bLpromoterwasmainlyconcentratedontheapicalpointoftheplumulewhereastheexpressiondrivenbyCaMV35Spromoterwasdistributedonnearlyallareasoftheelectroporatedtissues.Theseresultsindicatedthattheoptimizedembryoelectroporationconditionscouldbeusednotonlyingenetictransformationofindicaricebutalsoinassayofgeneregulationonembryos.
简介:Asimplemethodtocreateachromosome-specificDNAlibrqaryofrice,includingmicrodissection,amplification,charterizationandcloning,isdescribed.Ricechromosome4fromametaphasecellhasbeenisolatedandamplifiedbytheLinkerAdapterPCR(LA-PCR).ThePCRproductswerelabeledasprobeswithDIG-11-dUTPusingtherandomprimingmethod.SouthernblotanalysiswithricegenomicDNAandspecificRFLPmarkersdemonstratedthatthePCRproductswerederivedfromricechromosome4.Alargelibrarycomprisingover100,000recombinantplasmidmicroclonesfromricechromosome4wasconstructed.Colonyhybridizationshowedthat58%oftheclonescontainedsingleorlow-copysequencesand42%containedrepetitivesequences.ThesizeofinsertsgeneratedbyPCRrangedfrom140bpto500bp.ThismethodwillfacilitatecloningofthespecificchromosomeDNAmarkersandimportantgenesofrice.
简介:ToinvestigategeneticdiversitiesamongtheAAgenomeOryzaspeciesintheSoutheastandSouthAsia,atotalof428accessionsoftheAAgenomeOryzaspeciesweregenotypedusing36simplesequencerepeats(SSR)markersdistributedthroughoutthericegenome.Allofthe36SSRmarkersgeneratedpolymorphicbands,revealing100%polymorphism.Thenumberofallelesperlocusrangedfrom3to17withthemeanof8.6.TheNei’sgeneticdiversityindex(He)rangedfrom0.337atRM455to0.865atRM169withanaveragevalueof0.650.ThegeneticdiversityoftheAAgenomeOryzaspeciesintheSoutheastAsiawasobviouslyhigherthanthatintheSouthAsia.AmongthedetectedOryzaspeciesintheSouthandSoutheastAsia,O.rufipogonshowedthehighestgeneticdiversity.Meanwhile,ahighergeneticdifferentiation(Fst)wasfoundamongthedetectedOryzaspeciesintheSoutheastAsiathanintheSouthAsia.TheFstvaluebetweenO.nivaraandO.sativawasthehighest.Theresultsfromthenumberofspecificalleles,specificloci,andallelefrequencyconfirmedthegreatergeneticvariationamongthedetectedspecies.Inaddition,thespecificalleleinRM161displayedhigherfrequency(0.193),suggestingitsimportantfunctioninidentifyingOryzaspeciesofAAgenome.
简介:Potexperimentswereconductedin2002and2003toinvestigatetheeffectsofsoilcopper(Cu)concentrationongrowth,developmentandyieldformationofricebyusingthejaponicacultivarWuxiangjing14andhybridricecombinationShanyou63.Theplantheight,leafnumber,elongatedinternodenumberandheadingdateofriceplantswerenotaffectedatsoilCulevelsbelow200mg/kg,butaffectedsignificantlyatabove400mg/kg.TheinhibitoryeffectsonricegrowthanddevelopmentwereincreasedwiththeincrementofsoilCulevels.ThegrainyieldsdecreasedsignificantlywithraisingsoilCulevels.ThemainreasonsforthegrainyieldreductionsunderlowersoilCulevels(100,200mg/kg)weremainlyduetothedecreaseofnumberofspikeletsperpanicle,however,underhighersoilCulevels(morethan400mg/kg),bothpaniclenumberandnumberofspikeletsperpaniclecontributedtotheyieldloss.ThedecreasesofpaniclenumberbyCustressweremainlyattributedtoslowrecoveryfromtransplanting,delayedtilleringandreducedmaximumtillernumbers.ThereductionofnumberofspikeletsperpanicleundersoilGustressresultedfromthedecreasesofbothshootdryweight(SDW)attheheadingdateandtheratioofspikeletstoSDW.TotalbiomassatmaturitydecreasedsignificantlywiththeincreaseofsoilCulevels,whileeconomiccoefficientshowednon-significantdecreaseexceptundersoilCulevelsabove800mg/kg.