简介：一个适当小动物模型的缺乏仍然是一个主要障碍因为学习immunotolerance和immunopathogenesis由肝炎B病毒(HBV)导致了感染。在这研究，我们与recombinant在感染以后与持续HBVviremia报导一个老鼠模型带一个可复制的HBV染色体(AAV/HBV)的联系adeno的病毒(AAV)。类似于临床的HBV搬运人，感染AAV/HBV的老鼠为对HBV表面抗原(HBsAg)的抗体是sero否定的。有面对铝助手疫苗的常规HBV的免疫没能在这些老鼠对HBV得到有免疫力的回答。为了识别一支疫苗，那能潜在地围绕这忍耐，TLR9收缩筋CpG作为一个助手被加到HBsAg。有HBsAg/CpG的老鼠的种痘导致了viremia，而且强壮的抗体生产和T房间回答的不仅清理。而且，DNA复制和HBV的蛋白质表示显著地在AAV/HBV-infected鼠标的肝被减少。因此，AAV/HBV-infected老鼠可以作为一个柔韧的模型被使用调查HBVimmunotolerance并且为开发新奇免疫疗法根除的内在的机制HBV感染。

简介：Objective:Toestablishananimalmodeloflike-auditoryneuropathyinneonatalrat.MethodsTheani-malswereinjectedwithphenylhydrazinehydrochlorideorsalineat7-dayofage.ABRandDPOAEwereperformedtoassesstheauditoryfunction.Thecochleabasilarmembranestretchedpreparationandcochlearfrozensectionswerepreparedforimmunohistochemicalstainingtoexaminethemorphologicalchangeofhaircellsandspiralganglioncells(SGNs).ResultsAt7-dayagetheABRwaveI,III,V,latenciesandI-III,I-VIWIsintheexperimentalgroupweresignificantlyprolongedcomparedwiththoseinthecontrolgroup.TheABRthresholdswerealsoelevatedintheexperimentalgroup.Wefoundthereisnosignificantdiffer-enceinDPOAEinphenylhydrazinehydrochlorideexposuregroupcomparetocontrolgroup.Thecochlearhaircellsshowednosignsoflossinbothgroup,butthetotalnumberofneurofilamentspositivecellsinSGNsweresignificantlyreducedinthephenylhydrazinetreatedanimals.ConclusionOurstudysuggeststhatphenylhydrazinehydrochloridecanchangetheauditoryfunctionandinduceperipheralnervepathologybytargetedmainlytheSGNsinneonatalrat.

简介：Drugscancauseobviousdamagetothebrain.Toverifytherelationshipbetweenacupuncture,neurotrophicfactorexpressionandbraincellstructuralchanges,thisstudyestablishedaratmodelofheroinrelapseusingintramuscularinjectionofincreasingamountsofheroin.Duringthedetoxificationperiod,ratmodelsreceivedacupunctureatBaihui(DU20)andDazhui(DU14).Electronmicroscopydemonstratedthatthestructureoftheventraltegmentalareainheroinrelapseratsgraduallybecamenormalizedafteracupuncturetreatment.Immunohistochemicalstainingexhibitedthattheexpressionofbrain-derivedneurotrophicfactorandglialcellline-derivedneurotrophicfactorincreasedintheventraltegmentalareafollowingacupuncture.Moreover,theeffectsweresimilartothatofmethadone,atypeofmedicinecalledanopioid.ResultssuggestedthatacupunctureatBaihuiandDazhuiprotectedbrainneuronsagainstinjuryinratswithheroinrelapsebypromotingbrain-derivedneurotrophicfactorandglialcellline-derivedneurotrophicfactorexpression.

简介：AIM:ToevaluatetheeffectofOcimumsanctumleafextractonthedietarysupplementationinthetransgenicDrosophilamodelofParkinson’sdisease.METHOD:TheeffectofOcimumsanctumleafextractwasstudiedonthetransgenicDrosophilamodeloffliesexpressingnormalhumanalphasynuclein(h-αs)intheneurons.O.sanctumextractatfinalconcentrationsof0.0428×10-4,0.87×10-4,and1.85×10-4g·mL-1ofdietwereestablishedandtheflieswereallowedtofeedfor21days.Theclimbingassayandlipidperoxidationweretakenasparametersforthestudy.RESULTS:ThesupplementationofO.sanctumextractshowedadose-dependentsignificantdelayinthelossofclimbingabilityandreductioninoxidativestressinthebrainofPDmodelflies.CONCLUSION:TheresultsofthepresentstudyshowedthattheO.sanctumextractispotentinreducingthePDsymptomsintransgenicDrosophilamodel.

简介：Despitetheadvancesincombinatorialorsyntheticchemistryandbioinformatics,recentliteraturehasdemonstratedtherelevanceofnatureandbiomassasasourceofnewmoleculestotreatdifferentpathologies,i.e.,bioactivecompoundsobtainedfromEcteinascidiaturbinatetotreatsometypesofcancerorrapamycinfromStreptomyceshygroscopicustopreventorganrejectionaftertransplant.Thistrend

简介：BackgroundAnimalmodelsofmyocardialinfarction(MI)havebeenwidelyusedtostudythepathologicalandphysiologicalchangesthatoccurinMI,andtoobjectivelyevaluatetheefficacyofnewtreatments.Theyareanimportanttoolinthisprocedure.However,themortalityrateofMIanimalmodelshassofarbeenhigherthaninreal-lifesituations.Theaimofthisstudywastoexploretheuseofamodifiedretrogradetractiontrachealintubation(MRTI)methodforincreasingthesuccessrateofMImodelsinrats.MethodsSixtymaleSprague-Dawleyratswereusedintheexperiment.UsingtheMRTImethodofartificialairwaygeneration,weestablishedtheMImodelbyligationoftheleftanteriordescendingbranchofthecoronaryartery.WeanalyzedtheeffectsofMRTI,theuseoflidocaine,operativedetails,nursingconsiderationsduringtheoperation,andpost-operativefactorsonthesuccessrateoftheMImodelinrats.ResultsThesuccessrateofgeneratinganMImodelinratscanbesignificantlyincreasedusingthefollowingmethods:1)SettinguptheartificialairwaythroughtheuseofMRTIbyusingasingle-lumencentralvenouscatheter;2)Selectingaligationsite2mmbelowthemidpointoftheconnectionbetweentheleftatrialappendageandthepulmonarycone;3)Addingadropoflidocainetothesurfaceofthehearttoslowdowntheheartrate,maketheoperationeasiertoperform,andpreventarrhythmiaspostoperatively;4)Clearingupairwaysecretionstimelybothintraandpostoperatively;5)Makingsurethatratsareinawarmstatebothintraandpostoperatively;6)Preventingwoundinfection.ConclusionsUseoftheMRTImethodcanquicklyestablishanartificialairwayinrats.Intraoperativeuseoflidocaine,selectingaprecisevascularligationsite,andappropriatecarebothintraandpostoperativelycanincreasethesuccessrateofMImodelgeneration.更多还原